Experiment: Bacitracin 0.25 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_0215 and Shew_0216 are separated by 52 nucleotides Shew_0216 and Shew_0217 are separated by 74 nucleotides Shew_0217 and Shew_0218 are separated by 8 nucleotides Shew_0218 and Shew_0219 are separated by 211 nucleotides
Shew_0215: Shew_0215 - sporulation domain-containing protein (RefSeq), at 230,408 to 231,943
_0215
Shew_0216: Shew_0216 - DNA adenine methylase (RefSeq), at 231,996 to 232,835
_0216
Shew_0217: Shew_0217 - hypothetical protein (RefSeq), at 232,910 to 233,083
_0217
Shew_0218: Shew_0218 - hypothetical protein (RefSeq), at 233,092 to 233,436
_0218
Shew_0219: Shew_0219 - ribulose-phosphate 3-epimerase (RefSeq), at 233,648 to 234,322
_0219
Position (kb) 231
232
233 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 231.046 kb on - strand, within Shew_0215 at 231.287 kb on - strand, within Shew_0215 at 231.450 kb on + strand, within Shew_0215 at 231.463 kb on + strand, within Shew_0215 at 231.577 kb on + strand, within Shew_0215 at 231.580 kb on - strand, within Shew_0215 at 231.945 kb on + strand at 232.439 kb on + strand, within Shew_0216 at 232.497 kb on - strand, within Shew_0216 at 232.877 kb on + strand at 232.981 kb on + strand, within Shew_0217 at 233.086 kb on - strand at 233.147 kb on + strand, within Shew_0218 at 233.194 kb on + strand, within Shew_0218 at 233.370 kb on - strand, within Shew_0218 at 233.425 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Bacitracin 0.25 mg/ml remove 231,046 - Shew_0215 0.42 +0.2 231,287 - Shew_0215 0.57 -0.1 231,450 + Shew_0215 0.68 +0.1 231,463 + Shew_0215 0.69 -0.1 231,577 + Shew_0215 0.76 +0.1 231,580 - Shew_0215 0.76 -0.2 231,945 + -1.1 232,439 + Shew_0216 0.53 -1.2 232,497 - Shew_0216 0.60 -4.3 232,877 + +0.3 232,981 + Shew_0217 0.41 -0.4 233,086 - +0.1 233,147 + Shew_0218 0.16 +0.1 233,194 + Shew_0218 0.30 -0.9 233,370 - Shew_0218 0.81 -0.7 233,425 - -0.7
Or see this region's nucleotide sequence