Experiment: Bacitracin 0.25 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt argC and Shew_0203 are separated by 28 nucleotides Shew_0203 and Shew_0204 are separated by 42 nucleotides Shew_0204 and Shew_0205 are separated by 53 nucleotides
Shew_0202: argC - N-acetyl-gamma-glutamyl-phosphate reductase (RefSeq), at 215,298 to 216,278
argC
Shew_0203: Shew_0203 - acetylglutamate kinase (RefSeq), at 216,307 to 217,092
_0203
Shew_0204: Shew_0204 - ornithine carbamoyltransferase (RefSeq), at 217,135 to 218,040
_0204
Shew_0205: Shew_0205 - argininosuccinate synthase (RefSeq), at 218,094 to 219,317
_0205
Position (kb) 217
218
219 Strain fitness (log2 ratio) -2
-1
0
1 at 216.145 kb on + strand, within argC at 216.358 kb on - strand at 216.360 kb on + strand at 216.465 kb on + strand, within Shew_0203 at 216.473 kb on - strand, within Shew_0203 at 216.495 kb on + strand, within Shew_0203 at 216.511 kb on - strand, within Shew_0203 at 216.706 kb on + strand, within Shew_0203 at 216.786 kb on + strand, within Shew_0203 at 216.865 kb on + strand, within Shew_0203 at 216.908 kb on - strand, within Shew_0203 at 216.914 kb on + strand, within Shew_0203 at 216.932 kb on - strand, within Shew_0203 at 216.957 kb on + strand, within Shew_0203 at 216.957 kb on + strand, within Shew_0203 at 216.963 kb on + strand, within Shew_0203 at 216.973 kb on + strand, within Shew_0203 at 217.077 kb on + strand at 217.263 kb on - strand, within Shew_0204 at 217.373 kb on - strand, within Shew_0204 at 217.517 kb on - strand, within Shew_0204 at 217.826 kb on + strand, within Shew_0204 at 218.237 kb on + strand, within Shew_0205 at 218.822 kb on + strand, within Shew_0205 at 218.822 kb on + strand, within Shew_0205 at 218.822 kb on + strand, within Shew_0205 at 218.822 kb on + strand, within Shew_0205 at 218.830 kb on - strand, within Shew_0205 at 218.944 kb on + strand, within Shew_0205
Per-strain Table
Position Strand Gene LocusTag Fraction Bacitracin 0.25 mg/ml remove 216,145 + argC Shew_0202 0.86 -0.1 216,358 - +0.5 216,360 + +0.3 216,465 + Shew_0203 0.20 +0.0 216,473 - Shew_0203 0.21 +0.5 216,495 + Shew_0203 0.24 +0.2 216,511 - Shew_0203 0.26 +1.1 216,706 + Shew_0203 0.51 +0.8 216,786 + Shew_0203 0.61 +0.4 216,865 + Shew_0203 0.71 -0.1 216,908 - Shew_0203 0.76 -0.0 216,914 + Shew_0203 0.77 -0.4 216,932 - Shew_0203 0.80 -0.4 216,957 + Shew_0203 0.83 -0.8 216,957 + Shew_0203 0.83 +0.4 216,963 + Shew_0203 0.83 +0.2 216,973 + Shew_0203 0.85 +0.1 217,077 + -0.3 217,263 - Shew_0204 0.14 +0.1 217,373 - Shew_0204 0.26 -0.7 217,517 - Shew_0204 0.42 -1.8 217,826 + Shew_0204 0.76 -0.4 218,237 + Shew_0205 0.12 +1.0 218,822 + Shew_0205 0.59 +0.5 218,822 + Shew_0205 0.59 +0.2 218,822 + Shew_0205 0.59 +0.1 218,822 + Shew_0205 0.59 +0.1 218,830 - Shew_0205 0.60 +0.1 218,944 + Shew_0205 0.69 -0.3
Or see this region's nucleotide sequence