Experiment: Bacitracin 0.25 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_R0011 and Shew_R0012 are separated by 33 nucleotides Shew_R0012 and Shew_0197 are separated by 101 nucleotides Shew_0197 and Shew_0198 are separated by 217 nucleotides Shew_0198 and Shew_0199 are separated by 57 nucleotides Shew_0199 and Shew_0200 overlap by 1 nucleotides
Shew_R0011: Shew_R0011 - 5S ribosomal RNA (RefSeq), at 208,189 to 208,309
_R0011
Shew_R0012: Shew_R0012 - tRNA-Asp (RefSeq), at 208,343 to 208,419
_R0012
Shew_0197: Shew_0197 - hypothetical protein (RefSeq), at 208,521 to 209,021
_0197
Shew_0198: Shew_0198 - competence/damage-inducible protein CinA (RefSeq), at 209,239 to 210,513
_0198
Shew_0199: Shew_0199 - hypothetical protein (RefSeq), at 210,571 to 211,110
_0199
Shew_0200: Shew_0200 - phosphoenolpyruvate carboxylase (RefSeq), at 211,110 to 213,746
_0200
Position (kb) 209
210
211 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 208.791 kb on + strand, within Shew_0197 at 209.188 kb on + strand at 209.205 kb on - strand at 209.551 kb on - strand, within Shew_0198 at 209.646 kb on - strand, within Shew_0198 at 209.735 kb on - strand, within Shew_0198 at 209.857 kb on + strand, within Shew_0198 at 209.962 kb on + strand, within Shew_0198 at 210.152 kb on + strand, within Shew_0198 at 210.152 kb on + strand, within Shew_0198 at 210.420 kb on + strand at 210.449 kb on + strand at 210.457 kb on - strand at 210.719 kb on + strand, within Shew_0199 at 210.749 kb on - strand, within Shew_0199 at 210.777 kb on + strand, within Shew_0199 at 211.249 kb on - strand at 211.426 kb on - strand, within Shew_0200
Per-strain Table
Position Strand Gene LocusTag Fraction Bacitracin 0.25 mg/ml remove 208,791 + Shew_0197 0.54 -1.3 209,188 + -0.5 209,205 - +0.1 209,551 - Shew_0198 0.24 +0.4 209,646 - Shew_0198 0.32 -0.1 209,735 - Shew_0198 0.39 -3.1 209,857 + Shew_0198 0.48 -0.1 209,962 + Shew_0198 0.57 +1.1 210,152 + Shew_0198 0.72 -0.8 210,152 + Shew_0198 0.72 -0.2 210,420 + -2.5 210,449 + -0.2 210,457 - +0.1 210,719 + Shew_0199 0.27 +0.1 210,749 - Shew_0199 0.33 +1.6 210,777 + Shew_0199 0.38 +0.1 211,249 - +0.1 211,426 - Shew_0200 0.12 +0.8
Or see this region's nucleotide sequence