Experiment: Bacitracin 0.25 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_0070 and Shew_0071 are separated by 204 nucleotides Shew_0071 and Shew_0072 are separated by 7 nucleotides Shew_0072 and Shew_0073 are separated by 373 nucleotides
Shew_0070: Shew_0070 - iron-containing alcohol dehydrogenase (RefSeq), at 83,682 to 84,845
_0070
Shew_0071: Shew_0071 - AraC family transcriptional regulator (RefSeq), at 85,050 to 85,916
_0071
Shew_0072: Shew_0072 - hypothetical protein (RefSeq), at 85,924 to 86,445
_0072
Shew_0073: Shew_0073 - coproporphyrinogen III oxidase (RefSeq), at 86,819 to 88,162
_0073
Position (kb)
85
86 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 84.106 kb on + strand, within Shew_0070 at 84.204 kb on + strand, within Shew_0070 at 84.270 kb on - strand, within Shew_0070 at 84.282 kb on - strand, within Shew_0070 at 84.519 kb on - strand, within Shew_0070 at 84.540 kb on - strand, within Shew_0070 at 84.624 kb on - strand, within Shew_0070 at 84.688 kb on + strand, within Shew_0070 at 84.742 kb on - strand at 84.795 kb on - strand at 84.933 kb on - strand at 85.084 kb on + strand at 85.118 kb on - strand at 85.120 kb on + strand at 85.259 kb on + strand, within Shew_0071 at 85.267 kb on - strand, within Shew_0071 at 85.286 kb on + strand, within Shew_0071 at 85.385 kb on + strand, within Shew_0071 at 85.398 kb on - strand, within Shew_0071 at 85.460 kb on + strand, within Shew_0071 at 85.562 kb on + strand, within Shew_0071 at 85.570 kb on - strand, within Shew_0071 at 85.570 kb on - strand, within Shew_0071 at 85.585 kb on - strand, within Shew_0071 at 85.660 kb on + strand, within Shew_0071 at 85.696 kb on - strand, within Shew_0071 at 85.720 kb on + strand, within Shew_0071 at 85.728 kb on - strand, within Shew_0071 at 85.801 kb on + strand, within Shew_0071 at 85.894 kb on + strand at 86.224 kb on + strand, within Shew_0072 at 86.231 kb on + strand, within Shew_0072 at 86.231 kb on + strand, within Shew_0072 at 86.231 kb on + strand, within Shew_0072 at 86.231 kb on + strand, within Shew_0072 at 86.231 kb on + strand, within Shew_0072 at 86.234 kb on - strand, within Shew_0072 at 86.239 kb on - strand, within Shew_0072 at 86.292 kb on + strand, within Shew_0072 at 86.389 kb on + strand, within Shew_0072 at 86.676 kb on - strand at 86.823 kb on - strand at 86.869 kb on + strand at 86.882 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Bacitracin 0.25 mg/ml remove 84,106 + Shew_0070 0.36 -0.9 84,204 + Shew_0070 0.45 +2.4 84,270 - Shew_0070 0.51 -0.0 84,282 - Shew_0070 0.52 -0.3 84,519 - Shew_0070 0.72 +0.1 84,540 - Shew_0070 0.74 -0.2 84,624 - Shew_0070 0.81 +0.3 84,688 + Shew_0070 0.86 -0.5 84,742 - -0.2 84,795 - -0.1 84,933 - +0.2 85,084 + -0.5 85,118 - -0.6 85,120 + -2.0 85,259 + Shew_0071 0.24 -0.4 85,267 - Shew_0071 0.25 -0.4 85,286 + Shew_0071 0.27 -0.2 85,385 + Shew_0071 0.39 +0.2 85,398 - Shew_0071 0.40 -0.0 85,460 + Shew_0071 0.47 -1.8 85,562 + Shew_0071 0.59 +0.3 85,570 - Shew_0071 0.60 -0.3 85,570 - Shew_0071 0.60 -0.8 85,585 - Shew_0071 0.62 -0.1 85,660 + Shew_0071 0.70 +0.1 85,696 - Shew_0071 0.75 -0.1 85,720 + Shew_0071 0.77 -4.7 85,728 - Shew_0071 0.78 -0.5 85,801 + Shew_0071 0.87 +0.1 85,894 + -0.5 86,224 + Shew_0072 0.57 +1.1 86,231 + Shew_0072 0.59 +0.4 86,231 + Shew_0072 0.59 +0.8 86,231 + Shew_0072 0.59 -0.5 86,231 + Shew_0072 0.59 +0.3 86,231 + Shew_0072 0.59 -0.1 86,234 - Shew_0072 0.59 +0.2 86,239 - Shew_0072 0.60 -0.0 86,292 + Shew_0072 0.70 -0.3 86,389 + Shew_0072 0.89 -0.1 86,676 - -0.4 86,823 - +0.1 86,869 + +0.1 86,882 - -0.1
Or see this region's nucleotide sequence