Experiment: Bacitracin 0.25 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_0007 and Shew_0008 overlap by 4 nucleotides Shew_0008 and glyS are separated by 98 nucleotides glyS and glyQ are separated by 12 nucleotides
Shew_0007: Shew_0007 - urea amidolyase related protein (RefSeq), at 7,793 to 8,725
_0007
Shew_0008: Shew_0008 - allophanate hydrolase subunit 1 (RefSeq), at 8,722 to 9,408
_0008
Shew_0009: glyS - glycyl-tRNA synthetase subunit beta (RefSeq), at 9,507 to 11,576
glyS
Shew_0010: glyQ - glycyl-tRNA synthetase subunit alpha (RefSeq), at 11,589 to 12,494
glyQ
Position (kb)
9
10
11
12 Strain fitness (log2 ratio)
-2
-1
0
1 at 8.530 kb on - strand, within Shew_0007 at 8.615 kb on - strand, within Shew_0007 at 8.741 kb on + strand at 8.773 kb on - strand at 8.783 kb on - strand at 8.950 kb on - strand, within Shew_0008 at 9.065 kb on - strand, within Shew_0008 at 9.067 kb on + strand, within Shew_0008 at 9.106 kb on + strand, within Shew_0008 at 9.179 kb on + strand, within Shew_0008 at 9.179 kb on + strand, within Shew_0008 at 9.179 kb on + strand, within Shew_0008 at 9.187 kb on - strand, within Shew_0008 at 9.285 kb on + strand, within Shew_0008 at 9.292 kb on - strand, within Shew_0008 at 9.299 kb on + strand, within Shew_0008 at 9.299 kb on + strand, within Shew_0008 at 9.299 kb on + strand, within Shew_0008 at 9.299 kb on + strand, within Shew_0008 at 9.299 kb on + strand, within Shew_0008 at 9.299 kb on + strand, within Shew_0008 at 9.299 kb on + strand, within Shew_0008 at 9.299 kb on + strand, within Shew_0008 at 9.299 kb on + strand, within Shew_0008 at 9.299 kb on + strand, within Shew_0008 at 9.299 kb on + strand, within Shew_0008 at 9.299 kb on + strand, within Shew_0008 at 9.299 kb on + strand, within Shew_0008 at 9.299 kb on + strand, within Shew_0008 at 9.299 kb on + strand, within Shew_0008 at 9.307 kb on - strand, within Shew_0008 at 9.307 kb on - strand, within Shew_0008 at 9.307 kb on - strand, within Shew_0008 at 9.307 kb on - strand, within Shew_0008 at 9.307 kb on - strand, within Shew_0008 at 9.307 kb on - strand, within Shew_0008 at 9.307 kb on - strand, within Shew_0008 at 9.307 kb on - strand, within Shew_0008 at 9.307 kb on - strand, within Shew_0008
Per-strain Table
Position Strand Gene LocusTag Fraction Bacitracin 0.25 mg/ml remove 8,530 - Shew_0007 0.79 +0.6 8,615 - Shew_0007 0.88 -0.1 8,741 + +0.8 8,773 - +0.0 8,783 - +0.7 8,950 - Shew_0008 0.33 +0.6 9,065 - Shew_0008 0.50 +1.1 9,067 + Shew_0008 0.50 -0.4 9,106 + Shew_0008 0.56 -0.2 9,179 + Shew_0008 0.67 -0.7 9,179 + Shew_0008 0.67 -0.5 9,179 + Shew_0008 0.67 -0.0 9,187 - Shew_0008 0.68 -0.3 9,285 + Shew_0008 0.82 -0.1 9,292 - Shew_0008 0.83 -0.6 9,299 + Shew_0008 0.84 -0.1 9,299 + Shew_0008 0.84 -1.8 9,299 + Shew_0008 0.84 -1.0 9,299 + Shew_0008 0.84 -0.5 9,299 + Shew_0008 0.84 +0.5 9,299 + Shew_0008 0.84 +0.0 9,299 + Shew_0008 0.84 -0.1 9,299 + Shew_0008 0.84 +0.3 9,299 + Shew_0008 0.84 -0.0 9,299 + Shew_0008 0.84 -0.5 9,299 + Shew_0008 0.84 -0.2 9,299 + Shew_0008 0.84 +0.1 9,299 + Shew_0008 0.84 -0.3 9,299 + Shew_0008 0.84 -0.2 9,299 + Shew_0008 0.84 +0.1 9,307 - Shew_0008 0.85 -0.1 9,307 - Shew_0008 0.85 -0.5 9,307 - Shew_0008 0.85 -0.0 9,307 - Shew_0008 0.85 -0.5 9,307 - Shew_0008 0.85 +0.2 9,307 - Shew_0008 0.85 -1.6 9,307 - Shew_0008 0.85 +0.0 9,307 - Shew_0008 0.85 +0.2 9,307 - Shew_0008 0.85 +0.3
Or see this region's nucleotide sequence