Experiment: Formate with EDTA 3.9 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS05955 and MMP_RS05960 are separated by 12 nucleotides MMP_RS05960 and MMP_RS05965 are separated by 30 nucleotides MMP_RS05965 and MMP_RS05970 are separated by 13 nucleotides MMP_RS05970 and MMP_RS05975 are separated by 20 nucleotides MMP_RS05975 and MMP_RS05980 are separated by 22 nucleotides
MMP_RS05955: MMP_RS05955 - CoB--CoM heterodisulfide reductase subunit C, at 1,142,799 to 1,143,377
_RS05955
MMP_RS05960: MMP_RS05960 - CoB--CoM heterodisulfide reductase subunit B, at 1,143,390 to 1,144,295
_RS05960
MMP_RS05965: MMP_RS05965 - carboxymuconolactone decarboxylase family protein, at 1,144,326 to 1,144,640
_RS05965
MMP_RS05970: MMP_RS05970 - class II SORL domain-containing protein, at 1,144,654 to 1,145,010
_RS05970
MMP_RS05975: MMP_RS05975 - hypothetical protein, at 1,145,031 to 1,145,297
_RS05975
MMP_RS05980: MMP_RS05980 - ferritin, at 1,145,320 to 1,145,835
_RS05980
Position (kb)
1144
1145 Strain fitness (log2 ratio)
-1
0
1
2 at 1143.486 kb on + strand, within MMP_RS05960 at 1143.675 kb on + strand, within MMP_RS05960 at 1143.709 kb on - strand, within MMP_RS05960 at 1143.742 kb on + strand, within MMP_RS05960 at 1143.744 kb on + strand, within MMP_RS05960 at 1143.769 kb on - strand, within MMP_RS05960 at 1143.778 kb on - strand, within MMP_RS05960 at 1143.779 kb on + strand, within MMP_RS05960 at 1143.783 kb on - strand, within MMP_RS05960 at 1143.856 kb on + strand, within MMP_RS05960 at 1143.871 kb on - strand, within MMP_RS05960 at 1143.871 kb on - strand, within MMP_RS05960 at 1143.886 kb on - strand, within MMP_RS05960 at 1143.886 kb on - strand, within MMP_RS05960 at 1143.887 kb on + strand, within MMP_RS05960 at 1143.887 kb on + strand, within MMP_RS05960 at 1143.958 kb on - strand, within MMP_RS05960 at 1143.960 kb on - strand, within MMP_RS05960 at 1143.960 kb on - strand, within MMP_RS05960 at 1143.961 kb on + strand, within MMP_RS05960 at 1143.961 kb on + strand, within MMP_RS05960 at 1143.963 kb on - strand, within MMP_RS05960 at 1143.964 kb on + strand, within MMP_RS05960 at 1143.977 kb on - strand, within MMP_RS05960 at 1144.193 kb on + strand, within MMP_RS05960 at 1144.293 kb on - strand at 1144.544 kb on + strand, within MMP_RS05965 at 1144.580 kb on + strand, within MMP_RS05965 at 1144.580 kb on + strand, within MMP_RS05965 at 1144.653 kb on + strand at 1144.785 kb on + strand, within MMP_RS05970 at 1144.829 kb on - strand, within MMP_RS05970 at 1144.830 kb on + strand, within MMP_RS05970 at 1144.853 kb on - strand, within MMP_RS05970 at 1145.108 kb on - strand, within MMP_RS05975 at 1145.288 kb on - strand at 1145.292 kb on + strand at 1145.303 kb on + strand at 1145.319 kb on - strand at 1145.319 kb on - strand at 1145.320 kb on + strand at 1145.357 kb on - strand at 1145.357 kb on - strand at 1145.357 kb on - strand at 1145.358 kb on + strand at 1145.358 kb on + strand at 1145.362 kb on + strand at 1145.380 kb on - strand, within MMP_RS05980 at 1145.380 kb on - strand, within MMP_RS05980 at 1145.447 kb on - strand, within MMP_RS05980 at 1145.539 kb on + strand, within MMP_RS05980 at 1145.567 kb on - strand, within MMP_RS05980 at 1145.568 kb on + strand, within MMP_RS05980 at 1145.568 kb on + strand, within MMP_RS05980 at 1145.635 kb on + strand, within MMP_RS05980
Per-strain Table
Position Strand Gene LocusTag Fraction Formate with EDTA 3.9 mM remove 1,143,486 + MMP_RS05960 0.11 -0.2 1,143,675 + MMP_RS05960 0.31 -0.3 1,143,709 - MMP_RS05960 0.35 -0.4 1,143,742 + MMP_RS05960 0.39 +0.4 1,143,744 + MMP_RS05960 0.39 +0.5 1,143,769 - MMP_RS05960 0.42 +0.4 1,143,778 - MMP_RS05960 0.43 -0.2 1,143,779 + MMP_RS05960 0.43 +0.3 1,143,783 - MMP_RS05960 0.43 +0.3 1,143,856 + MMP_RS05960 0.51 +1.0 1,143,871 - MMP_RS05960 0.53 +0.9 1,143,871 - MMP_RS05960 0.53 +1.4 1,143,886 - MMP_RS05960 0.55 +0.1 1,143,886 - MMP_RS05960 0.55 -0.2 1,143,887 + MMP_RS05960 0.55 -0.4 1,143,887 + MMP_RS05960 0.55 -0.4 1,143,958 - MMP_RS05960 0.63 +0.4 1,143,960 - MMP_RS05960 0.63 -0.3 1,143,960 - MMP_RS05960 0.63 -0.2 1,143,961 + MMP_RS05960 0.63 -0.4 1,143,961 + MMP_RS05960 0.63 +0.4 1,143,963 - MMP_RS05960 0.63 -0.0 1,143,964 + MMP_RS05960 0.63 -0.3 1,143,977 - MMP_RS05960 0.65 -0.2 1,144,193 + MMP_RS05960 0.89 +0.3 1,144,293 - -0.0 1,144,544 + MMP_RS05965 0.69 -0.1 1,144,580 + MMP_RS05965 0.81 -0.4 1,144,580 + MMP_RS05965 0.81 -0.1 1,144,653 + +0.5 1,144,785 + MMP_RS05970 0.37 +0.2 1,144,829 - MMP_RS05970 0.49 +0.4 1,144,830 + MMP_RS05970 0.49 -0.3 1,144,853 - MMP_RS05970 0.56 -0.7 1,145,108 - MMP_RS05975 0.29 +0.3 1,145,288 - -0.0 1,145,292 + +0.0 1,145,303 + +0.3 1,145,319 - +0.2 1,145,319 - +0.8 1,145,320 + -0.1 1,145,357 - +0.2 1,145,357 - -1.0 1,145,357 - +0.5 1,145,358 + +0.2 1,145,358 + +1.8 1,145,362 + +0.2 1,145,380 - MMP_RS05980 0.12 -0.6 1,145,380 - MMP_RS05980 0.12 -0.3 1,145,447 - MMP_RS05980 0.25 +0.4 1,145,539 + MMP_RS05980 0.42 -0.0 1,145,567 - MMP_RS05980 0.48 +0.2 1,145,568 + MMP_RS05980 0.48 +0.0 1,145,568 + MMP_RS05980 0.48 -0.3 1,145,635 + MMP_RS05980 0.61 +0.0
Or see this region's nucleotide sequence