Experiment: Formate with EDTA 3.9 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS01630 and MMP_RS01635 are separated by 46 nucleotides MMP_RS01635 and MMP_RS01640 are separated by 5 nucleotides MMP_RS01640 and MMP_RS01645 are separated by 72 nucleotides MMP_RS01645 and MMP_RS01650 are separated by 156 nucleotides
MMP_RS01630: MMP_RS01630 - N-glycosylase/DNA lyase, at 301,670 to 302,428
_RS01630
MMP_RS01635: MMP_RS01635 - thiamine pyrophosphate-dependent enzyme, at 302,475 to 303,335
_RS01635
MMP_RS01640: MMP_RS01640 - 2-oxoacid:acceptor oxidoreductase subunit alpha, at 303,341 to 305,053
_RS01640
MMP_RS01645: MMP_RS01645 - SWIM zinc finger family protein, at 305,126 to 305,815
_RS01645
MMP_RS01650: MMP_RS01650 - sulfurtransferase TusA family protein, at 305,972 to 306,184
_RS01650
Position (kb) 303
304
305
306 Strain fitness (log2 ratio) -1
0
1 at 302.408 kb on - strand at 302.447 kb on - strand at 302.455 kb on + strand at 302.459 kb on + strand at 302.558 kb on + strand at 302.702 kb on + strand, within MMP_RS01635 at 302.748 kb on - strand, within MMP_RS01635 at 302.755 kb on + strand, within MMP_RS01635 at 302.958 kb on + strand, within MMP_RS01635 at 302.966 kb on + strand, within MMP_RS01635 at 302.966 kb on + strand, within MMP_RS01635 at 302.985 kb on - strand, within MMP_RS01635 at 303.047 kb on + strand, within MMP_RS01635 at 303.301 kb on + strand at 303.449 kb on - strand at 303.612 kb on - strand, within MMP_RS01640 at 304.001 kb on + strand, within MMP_RS01640 at 304.051 kb on + strand, within MMP_RS01640 at 304.052 kb on - strand, within MMP_RS01640 at 304.092 kb on + strand, within MMP_RS01640 at 304.160 kb on - strand, within MMP_RS01640 at 304.163 kb on - strand, within MMP_RS01640 at 304.284 kb on + strand, within MMP_RS01640 at 304.284 kb on + strand, within MMP_RS01640 at 304.313 kb on - strand, within MMP_RS01640 at 304.493 kb on - strand, within MMP_RS01640 at 304.773 kb on + strand, within MMP_RS01640 at 304.880 kb on - strand, within MMP_RS01640 at 304.943 kb on - strand at 304.943 kb on - strand at 304.975 kb on - strand at 305.071 kb on - strand at 305.078 kb on + strand at 305.096 kb on + strand at 305.097 kb on - strand at 305.097 kb on - strand at 305.097 kb on - strand at 305.098 kb on + strand at 305.139 kb on - strand at 305.139 kb on - strand at 305.139 kb on - strand at 305.139 kb on - strand at 305.140 kb on + strand at 305.140 kb on + strand at 305.140 kb on + strand at 305.140 kb on + strand at 305.141 kb on - strand at 305.142 kb on + strand at 305.142 kb on + strand at 305.142 kb on + strand at 305.380 kb on + strand, within MMP_RS01645 at 305.542 kb on + strand, within MMP_RS01645 at 305.625 kb on - strand, within MMP_RS01645 at 305.630 kb on - strand, within MMP_RS01645 at 305.773 kb on - strand at 305.792 kb on - strand at 305.792 kb on - strand at 305.884 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Formate with EDTA 3.9 mM remove 302,408 - -0.3 302,447 - -0.4 302,455 + -1.3 302,459 + -0.4 302,558 + -0.6 302,702 + MMP_RS01635 0.26 -0.3 302,748 - MMP_RS01635 0.32 -0.2 302,755 + MMP_RS01635 0.33 -0.3 302,958 + MMP_RS01635 0.56 -0.7 302,966 + MMP_RS01635 0.57 -0.2 302,966 + MMP_RS01635 0.57 +0.6 302,985 - MMP_RS01635 0.59 +0.7 303,047 + MMP_RS01635 0.66 -0.3 303,301 + -0.2 303,449 - -0.5 303,612 - MMP_RS01640 0.16 -0.5 304,001 + MMP_RS01640 0.39 -0.3 304,051 + MMP_RS01640 0.41 -0.3 304,052 - MMP_RS01640 0.42 -0.6 304,092 + MMP_RS01640 0.44 -0.6 304,160 - MMP_RS01640 0.48 -0.4 304,163 - MMP_RS01640 0.48 -0.0 304,284 + MMP_RS01640 0.55 +0.3 304,284 + MMP_RS01640 0.55 +0.1 304,313 - MMP_RS01640 0.57 +0.3 304,493 - MMP_RS01640 0.67 +0.2 304,773 + MMP_RS01640 0.84 -0.3 304,880 - MMP_RS01640 0.90 -0.5 304,943 - +0.1 304,943 - +0.3 304,975 - -0.2 305,071 - -0.1 305,078 + +0.0 305,096 + +0.5 305,097 - -0.3 305,097 - -0.0 305,097 - +0.2 305,098 + -0.8 305,139 - -0.0 305,139 - +0.4 305,139 - +0.2 305,139 - -0.2 305,140 + -0.1 305,140 + +0.1 305,140 + +0.4 305,140 + +0.8 305,141 - +0.1 305,142 + +0.2 305,142 + +0.2 305,142 + -0.0 305,380 + MMP_RS01645 0.37 -0.2 305,542 + MMP_RS01645 0.60 +0.7 305,625 - MMP_RS01645 0.72 -0.0 305,630 - MMP_RS01645 0.73 -0.2 305,773 - +1.4 305,792 - -0.3 305,792 - -0.1 305,884 + -0.7
Or see this region's nucleotide sequence