Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt recA and Shew_1216 are separated by 79 nucleotides Shew_1216 and Shew_1217 are separated by 244 nucleotides Shew_1217 and Shew_1218 are separated by 306 nucleotides Shew_1218 and Shew_R0026 are separated by 213 nucleotides Shew_R0026 and Shew_R0027 are separated by 21 nucleotides Shew_R0027 and Shew_R0028 are separated by 37 nucleotides
Shew_1215: recA - recombinase A (RefSeq), at 1,410,941 to 1,412,002
recA
Shew_1216: Shew_1216 - regulatory protein RecX (RefSeq), at 1,412,082 to 1,412,534
_1216
Shew_1217: Shew_1217 - alanyl-tRNA synthetase (RefSeq), at 1,412,779 to 1,415,403
_1217
Shew_1218: Shew_1218 - carbon storage regulator, CsrA (RefSeq), at 1,415,710 to 1,415,907
_1218
Shew_R0026: Shew_R0026 - tRNA-Ser (RefSeq), at 1,416,121 to 1,416,212
_R0026
Shew_R0027: Shew_R0027 - tRNA-Arg (RefSeq), at 1,416,234 to 1,416,310
_R0027
Shew_R0028: Shew_R0028 - tRNA-Arg (RefSeq), at 1,416,348 to 1,416,424
_R0028
Position (kb) 1412
1413
1414
1415
1416 Strain fitness (log2 ratio) -1
0
1 at 1412.153 kb on + strand, within Shew_1216 at 1412.291 kb on + strand, within Shew_1216 at 1412.294 kb on - strand, within Shew_1216 at 1412.304 kb on - strand, within Shew_1216 at 1412.455 kb on - strand, within Shew_1216 at 1415.469 kb on - strand at 1415.924 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 1,412,153 + Shew_1216 0.16 +0.3 1,412,291 + Shew_1216 0.46 -0.1 1,412,294 - Shew_1216 0.47 -0.2 1,412,304 - Shew_1216 0.49 -0.5 1,412,455 - Shew_1216 0.82 -0.3 1,415,469 - +0.6 1,415,924 + +0.2
Or see this region's nucleotide sequence