Experiment: Inosine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_0259 and Dshi_0260 are separated by 103 nucleotides Dshi_0260 and Dshi_0261 are separated by 1 nucleotides Dshi_0261 and Dshi_0262 are separated by 352 nucleotides
Dshi_0259: Dshi_0259 - NusG antitermination factor (RefSeq), at 256,673 to 257,206
_0259
Dshi_0260: Dshi_0260 - ribosomal protein L11 (RefSeq), at 257,310 to 257,735
_0260
Dshi_0261: Dshi_0261 - ribosomal protein L1 (RefSeq), at 257,737 to 258,435
_0261
Dshi_0262: Dshi_0262 - hypothetical protein (RefSeq), at 258,788 to 259,516
_0262
Position (kb)
257
258
259 Strain fitness (log2 ratio)
-2
-1
0
1 at 258.573 kb on + strand at 258.578 kb on + strand at 258.581 kb on - strand at 258.584 kb on - strand at 258.592 kb on - strand at 258.605 kb on + strand at 258.658 kb on - strand at 258.663 kb on + strand at 258.715 kb on - strand at 258.811 kb on + strand at 258.822 kb on + strand at 258.827 kb on + strand at 258.827 kb on + strand at 258.828 kb on + strand at 258.835 kb on - strand at 258.869 kb on + strand, within Dshi_0262 at 259.039 kb on + strand, within Dshi_0262 at 259.173 kb on + strand, within Dshi_0262 at 259.217 kb on + strand, within Dshi_0262 at 259.276 kb on + strand, within Dshi_0262 at 259.318 kb on - strand, within Dshi_0262
Per-strain Table
Position Strand Gene LocusTag Fraction Inosine (N) remove 258,573 + +1.5 258,578 + +0.4 258,581 - -0.1 258,584 - +1.1 258,592 - +1.1 258,605 + -0.5 258,658 - -1.4 258,663 + +0.3 258,715 - -2.0 258,811 + -0.3 258,822 + -0.1 258,827 + -0.1 258,827 + +0.3 258,828 + -0.9 258,835 - +0.6 258,869 + Dshi_0262 0.11 +0.4 259,039 + Dshi_0262 0.34 -0.5 259,173 + Dshi_0262 0.53 -0.3 259,217 + Dshi_0262 0.59 -0.4 259,276 + Dshi_0262 0.67 +0.3 259,318 - Dshi_0262 0.73 -1.3
Or see this region's nucleotide sequence