Experiment: Uridine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_1210 and Dshi_1211 are separated by 12 nucleotides Dshi_1211 and Dshi_1212 overlap by 4 nucleotides Dshi_1212 and Dshi_1213 are separated by 41 nucleotides Dshi_1213 and Dshi_1214 overlap by 4 nucleotides
Dshi_1210: Dshi_1210 - molybdopterin dehydrogenase FAD-binding (RefSeq), at 1,249,012 to 1,249,866
_1210
Dshi_1211: Dshi_1211 - (2Fe-2S)-binding domain protein (RefSeq), at 1,249,879 to 1,250,370
_1211
Dshi_1212: Dshi_1212 - carbon-monoxide dehydrogenase, large subunit (RefSeq), at 1,250,367 to 1,252,787
_1212
Dshi_1213: Dshi_1213 - ATPase associated with various cellular activities AAA_5 (RefSeq), at 1,252,829 to 1,253,737
_1213
Dshi_1214: Dshi_1214 - VWA containing CoxE family protein (RefSeq), at 1,253,734 to 1,254,912
_1214
Position (kb)
1250
1251
1252
1253 Strain fitness (log2 ratio)
-2
-1
0
1 at 1249.433 kb on - strand, within Dshi_1210 at 1249.543 kb on + strand, within Dshi_1210 at 1249.660 kb on - strand, within Dshi_1210 at 1249.660 kb on - strand, within Dshi_1210 at 1249.707 kb on - strand, within Dshi_1210 at 1249.833 kb on - strand at 1249.866 kb on + strand at 1249.893 kb on + strand at 1249.963 kb on - strand, within Dshi_1211 at 1250.001 kb on - strand, within Dshi_1211 at 1250.066 kb on - strand, within Dshi_1211 at 1250.082 kb on + strand, within Dshi_1211 at 1250.090 kb on - strand, within Dshi_1211 at 1250.280 kb on + strand, within Dshi_1211 at 1250.418 kb on + strand at 1250.418 kb on + strand at 1250.426 kb on - strand at 1250.532 kb on + strand at 1250.536 kb on + strand at 1250.626 kb on - strand, within Dshi_1212 at 1250.712 kb on - strand, within Dshi_1212 at 1250.755 kb on + strand, within Dshi_1212 at 1250.773 kb on - strand, within Dshi_1212 at 1251.041 kb on + strand, within Dshi_1212 at 1251.043 kb on - strand, within Dshi_1212 at 1251.268 kb on + strand, within Dshi_1212 at 1251.400 kb on + strand, within Dshi_1212 at 1251.518 kb on - strand, within Dshi_1212 at 1251.564 kb on + strand, within Dshi_1212 at 1251.642 kb on + strand, within Dshi_1212 at 1251.642 kb on + strand, within Dshi_1212 at 1251.699 kb on - strand, within Dshi_1212 at 1251.706 kb on - strand, within Dshi_1212 at 1251.732 kb on - strand, within Dshi_1212 at 1251.829 kb on + strand, within Dshi_1212 at 1251.945 kb on - strand, within Dshi_1212 at 1251.950 kb on - strand, within Dshi_1212 at 1252.004 kb on - strand, within Dshi_1212 at 1252.015 kb on - strand, within Dshi_1212 at 1252.069 kb on + strand, within Dshi_1212 at 1252.228 kb on - strand, within Dshi_1212 at 1252.251 kb on + strand, within Dshi_1212 at 1252.259 kb on - strand, within Dshi_1212 at 1252.705 kb on + strand at 1252.808 kb on + strand at 1252.815 kb on - strand at 1252.816 kb on - strand at 1252.863 kb on - strand at 1252.924 kb on + strand, within Dshi_1213 at 1253.021 kb on + strand, within Dshi_1213 at 1253.063 kb on - strand, within Dshi_1213 at 1253.322 kb on + strand, within Dshi_1213 at 1253.340 kb on - strand, within Dshi_1213 at 1253.621 kb on - strand, within Dshi_1213 at 1253.706 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Uridine (N) remove 1,249,433 - Dshi_1210 0.49 -0.1 1,249,543 + Dshi_1210 0.62 -0.9 1,249,660 - Dshi_1210 0.76 -1.6 1,249,660 - Dshi_1210 0.76 -0.9 1,249,707 - Dshi_1210 0.81 -0.0 1,249,833 - +0.9 1,249,866 + +0.4 1,249,893 + +0.3 1,249,963 - Dshi_1211 0.17 +1.6 1,250,001 - Dshi_1211 0.25 +1.2 1,250,066 - Dshi_1211 0.38 +0.0 1,250,082 + Dshi_1211 0.41 -0.4 1,250,090 - Dshi_1211 0.43 -0.6 1,250,280 + Dshi_1211 0.82 -0.1 1,250,418 + -0.4 1,250,418 + -1.1 1,250,426 - +0.3 1,250,532 + +0.2 1,250,536 + -1.3 1,250,626 - Dshi_1212 0.11 -0.3 1,250,712 - Dshi_1212 0.14 +0.0 1,250,755 + Dshi_1212 0.16 -0.5 1,250,773 - Dshi_1212 0.17 -0.1 1,251,041 + Dshi_1212 0.28 -1.2 1,251,043 - Dshi_1212 0.28 -0.1 1,251,268 + Dshi_1212 0.37 +0.8 1,251,400 + Dshi_1212 0.43 -0.8 1,251,518 - Dshi_1212 0.48 +0.6 1,251,564 + Dshi_1212 0.49 -1.1 1,251,642 + Dshi_1212 0.53 -0.3 1,251,642 + Dshi_1212 0.53 +0.2 1,251,699 - Dshi_1212 0.55 +0.4 1,251,706 - Dshi_1212 0.55 -1.2 1,251,732 - Dshi_1212 0.56 +0.5 1,251,829 + Dshi_1212 0.60 -0.6 1,251,945 - Dshi_1212 0.65 +0.4 1,251,950 - Dshi_1212 0.65 -0.2 1,252,004 - Dshi_1212 0.68 -2.6 1,252,015 - Dshi_1212 0.68 +0.1 1,252,069 + Dshi_1212 0.70 +0.4 1,252,228 - Dshi_1212 0.77 +0.9 1,252,251 + Dshi_1212 0.78 -0.4 1,252,259 - Dshi_1212 0.78 -0.1 1,252,705 + +0.4 1,252,808 + -0.1 1,252,815 - +0.4 1,252,816 - -0.3 1,252,863 - +0.1 1,252,924 + Dshi_1213 0.10 -1.1 1,253,021 + Dshi_1213 0.21 +0.1 1,253,063 - Dshi_1213 0.26 -0.3 1,253,322 + Dshi_1213 0.54 -0.1 1,253,340 - Dshi_1213 0.56 -0.6 1,253,621 - Dshi_1213 0.87 -0.9 1,253,706 - -0.4
Or see this region's nucleotide sequence