Experiment: tungstate 0.05 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_678 and Pf6N2E2_679 overlap by 4 nucleotides Pf6N2E2_679 and Pf6N2E2_680 are separated by 191 nucleotides Pf6N2E2_680 and Pf6N2E2_681 overlap by 4 nucleotides
Pf6N2E2_678 - Putative oxidoreductase in 4-hydroxyproline catabolic gene cluster, at 783,039 to 784,295
_678
Pf6N2E2_679 - Hypothetical, similar to sarcosine oxidase alpha subunit, 2Fe-2S domain, at 784,292 to 784,528
_679
Pf6N2E2_680 - D-amino-acid oxidase (EC 1.4.3.3), at 784,720 to 785,835
_680
Pf6N2E2_681 - 4-hydroxyproline epimerase (EC 5.1.1.8), at 785,832 to 786,764
_681
Position (kb)
784
785
786 Strain fitness (log2 ratio)
-2
-1
0
1 at 784.000 kb on + strand, within Pf6N2E2_678 at 784.001 kb on - strand, within Pf6N2E2_678 at 784.047 kb on - strand, within Pf6N2E2_678 at 784.089 kb on + strand, within Pf6N2E2_678 at 784.089 kb on + strand, within Pf6N2E2_678 at 784.090 kb on - strand, within Pf6N2E2_678 at 784.090 kb on - strand, within Pf6N2E2_678 at 784.090 kb on - strand, within Pf6N2E2_678 at 784.090 kb on - strand, within Pf6N2E2_678 at 784.387 kb on + strand, within Pf6N2E2_679 at 784.387 kb on + strand, within Pf6N2E2_679 at 784.517 kb on + strand at 784.518 kb on - strand at 784.760 kb on + strand at 784.760 kb on + strand at 785.092 kb on + strand, within Pf6N2E2_680 at 785.092 kb on + strand, within Pf6N2E2_680 at 785.092 kb on + strand, within Pf6N2E2_680 at 785.092 kb on + strand, within Pf6N2E2_680 at 785.092 kb on + strand, within Pf6N2E2_680 at 785.093 kb on - strand, within Pf6N2E2_680 at 785.093 kb on - strand, within Pf6N2E2_680 at 785.093 kb on - strand, within Pf6N2E2_680 at 785.093 kb on - strand, within Pf6N2E2_680 at 785.093 kb on - strand, within Pf6N2E2_680 at 785.093 kb on - strand, within Pf6N2E2_680 at 785.093 kb on - strand, within Pf6N2E2_680 at 785.094 kb on + strand, within Pf6N2E2_680 at 785.094 kb on + strand, within Pf6N2E2_680 at 785.095 kb on - strand, within Pf6N2E2_680 at 785.095 kb on - strand, within Pf6N2E2_680 at 785.142 kb on - strand, within Pf6N2E2_680 at 785.142 kb on - strand, within Pf6N2E2_680 at 785.397 kb on - strand, within Pf6N2E2_680 at 785.522 kb on + strand, within Pf6N2E2_680 at 785.522 kb on + strand, within Pf6N2E2_680 at 785.523 kb on - strand, within Pf6N2E2_680 at 785.947 kb on + strand, within Pf6N2E2_681 at 785.947 kb on + strand, within Pf6N2E2_681 at 786.073 kb on + strand, within Pf6N2E2_681 at 786.074 kb on - strand, within Pf6N2E2_681 at 786.093 kb on + strand, within Pf6N2E2_681 at 786.093 kb on + strand, within Pf6N2E2_681 at 786.198 kb on + strand, within Pf6N2E2_681 at 786.206 kb on - strand, within Pf6N2E2_681 at 786.206 kb on - strand, within Pf6N2E2_681 at 786.206 kb on - strand, within Pf6N2E2_681 at 786.211 kb on + strand, within Pf6N2E2_681 at 786.355 kb on + strand, within Pf6N2E2_681 at 786.355 kb on + strand, within Pf6N2E2_681 at 786.408 kb on + strand, within Pf6N2E2_681 at 786.832 kb on - strand at 786.832 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction tungstate 0.05 M remove 784,000 + Pf6N2E2_678 0.76 +0.0 784,001 - Pf6N2E2_678 0.77 -0.2 784,047 - Pf6N2E2_678 0.80 -0.6 784,089 + Pf6N2E2_678 0.84 +1.7 784,089 + Pf6N2E2_678 0.84 -0.4 784,090 - Pf6N2E2_678 0.84 +0.4 784,090 - Pf6N2E2_678 0.84 +0.0 784,090 - Pf6N2E2_678 0.84 -0.1 784,090 - Pf6N2E2_678 0.84 +0.1 784,387 + Pf6N2E2_679 0.40 +1.6 784,387 + Pf6N2E2_679 0.40 +0.3 784,517 + -0.2 784,518 - +0.8 784,760 + +0.4 784,760 + -1.2 785,092 + Pf6N2E2_680 0.33 -0.1 785,092 + Pf6N2E2_680 0.33 +0.8 785,092 + Pf6N2E2_680 0.33 -0.7 785,092 + Pf6N2E2_680 0.33 +0.3 785,092 + Pf6N2E2_680 0.33 +0.2 785,093 - Pf6N2E2_680 0.33 -0.6 785,093 - Pf6N2E2_680 0.33 -0.7 785,093 - Pf6N2E2_680 0.33 +0.1 785,093 - Pf6N2E2_680 0.33 -0.6 785,093 - Pf6N2E2_680 0.33 +0.0 785,093 - Pf6N2E2_680 0.33 -0.1 785,093 - Pf6N2E2_680 0.33 -0.5 785,094 + Pf6N2E2_680 0.34 -1.4 785,094 + Pf6N2E2_680 0.34 -0.1 785,095 - Pf6N2E2_680 0.34 -1.1 785,095 - Pf6N2E2_680 0.34 -1.9 785,142 - Pf6N2E2_680 0.38 -0.6 785,142 - Pf6N2E2_680 0.38 +0.0 785,397 - Pf6N2E2_680 0.61 -0.4 785,522 + Pf6N2E2_680 0.72 +1.0 785,522 + Pf6N2E2_680 0.72 -0.1 785,523 - Pf6N2E2_680 0.72 -0.1 785,947 + Pf6N2E2_681 0.12 +0.9 785,947 + Pf6N2E2_681 0.12 +0.6 786,073 + Pf6N2E2_681 0.26 -0.1 786,074 - Pf6N2E2_681 0.26 +1.0 786,093 + Pf6N2E2_681 0.28 +0.4 786,093 + Pf6N2E2_681 0.28 -0.0 786,198 + Pf6N2E2_681 0.39 -0.3 786,206 - Pf6N2E2_681 0.40 -1.1 786,206 - Pf6N2E2_681 0.40 -0.8 786,206 - Pf6N2E2_681 0.40 -0.1 786,211 + Pf6N2E2_681 0.41 +0.1 786,355 + Pf6N2E2_681 0.56 +0.3 786,355 + Pf6N2E2_681 0.56 +0.6 786,408 + Pf6N2E2_681 0.62 -1.1 786,832 - +0.5 786,832 - -0.3
Or see this region's nucleotide sequence