Experiment: tungstate 0.05 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_58 and Pf6N2E2_59 are separated by 120 nucleotides Pf6N2E2_59 and Pf6N2E2_60 are separated by 193 nucleotides Pf6N2E2_60 and Pf6N2E2_61 are separated by 69 nucleotides Pf6N2E2_61 and Pf6N2E2_62 overlap by 31 nucleotides
Pf6N2E2_58 - tRNA-dihydrouridine synthase C (EC 1.-.-.-), at 96,886 to 97,845
_58
Pf6N2E2_59 - 16 kDa heat shock protein A, at 97,966 to 98,418
_59
Pf6N2E2_60 - Sensory box/GGDEF family protein, at 98,612 to 101,890
_60
Pf6N2E2_61 - Cys regulon transcriptional activator CysB, at 101,960 to 102,238
_61
Pf6N2E2_62 - Cys regulon transcriptional activator CysB, at 102,208 to 102,852
_62
Position (kb) 98
99
100
101
102 Strain fitness (log2 ratio) -2
-1
0
1 at 97.889 kb on + strand at 97.889 kb on + strand at 97.889 kb on + strand at 97.889 kb on + strand at 97.889 kb on + strand at 97.889 kb on + strand at 97.889 kb on + strand at 97.894 kb on - strand at 97.894 kb on - strand at 97.971 kb on + strand at 98.072 kb on - strand, within Pf6N2E2_59 at 98.072 kb on - strand, within Pf6N2E2_59 at 98.080 kb on + strand, within Pf6N2E2_59 at 98.080 kb on + strand, within Pf6N2E2_59 at 98.080 kb on + strand, within Pf6N2E2_59 at 98.080 kb on + strand, within Pf6N2E2_59 at 98.081 kb on - strand, within Pf6N2E2_59 at 98.227 kb on + strand, within Pf6N2E2_59 at 98.227 kb on + strand, within Pf6N2E2_59 at 98.228 kb on - strand, within Pf6N2E2_59 at 98.462 kb on + strand at 98.468 kb on + strand at 98.468 kb on + strand at 98.469 kb on - strand at 98.469 kb on - strand at 98.531 kb on - strand at 98.531 kb on - strand at 98.531 kb on - strand at 98.531 kb on - strand at 98.546 kb on + strand at 98.547 kb on - strand at 98.547 kb on - strand at 98.547 kb on - strand at 98.614 kb on - strand at 98.664 kb on + strand at 98.664 kb on + strand at 98.665 kb on - strand at 98.673 kb on + strand at 98.674 kb on - strand at 98.674 kb on - strand at 98.674 kb on - strand at 98.675 kb on + strand at 98.675 kb on + strand at 98.675 kb on + strand at 98.675 kb on + strand at 98.675 kb on + strand at 98.675 kb on + strand at 98.675 kb on + strand at 98.675 kb on + strand at 98.675 kb on + strand at 98.675 kb on + strand at 98.675 kb on + strand at 98.676 kb on - strand at 98.676 kb on - strand at 98.676 kb on - strand at 98.676 kb on - strand at 98.676 kb on - strand at 98.676 kb on - strand at 98.676 kb on - strand at 98.676 kb on - strand at 98.676 kb on - strand at 98.676 kb on - strand at 98.676 kb on - strand at 98.676 kb on - strand at 98.676 kb on - strand at 98.676 kb on - strand at 99.034 kb on + strand, within Pf6N2E2_60 at 99.035 kb on - strand, within Pf6N2E2_60 at 99.035 kb on - strand, within Pf6N2E2_60 at 99.035 kb on - strand, within Pf6N2E2_60 at 99.450 kb on + strand, within Pf6N2E2_60 at 99.516 kb on - strand, within Pf6N2E2_60 at 100.141 kb on - strand, within Pf6N2E2_60 at 100.414 kb on + strand, within Pf6N2E2_60 at 100.414 kb on + strand, within Pf6N2E2_60 at 100.414 kb on + strand, within Pf6N2E2_60 at 100.503 kb on - strand, within Pf6N2E2_60 at 100.776 kb on + strand, within Pf6N2E2_60 at 100.809 kb on + strand, within Pf6N2E2_60 at 100.809 kb on + strand, within Pf6N2E2_60 at 100.821 kb on + strand, within Pf6N2E2_60 at 100.821 kb on + strand, within Pf6N2E2_60 at 100.822 kb on - strand, within Pf6N2E2_60 at 100.822 kb on - strand, within Pf6N2E2_60 at 101.101 kb on + strand, within Pf6N2E2_60 at 101.101 kb on + strand, within Pf6N2E2_60 at 101.241 kb on - strand, within Pf6N2E2_60 at 101.331 kb on + strand, within Pf6N2E2_60 at 101.649 kb on - strand at 101.661 kb on + strand at 101.662 kb on - strand at 101.662 kb on - strand at 101.891 kb on + strand at 101.921 kb on + strand at 101.922 kb on - strand at 101.961 kb on + strand at 101.961 kb on + strand at 101.961 kb on + strand at 101.961 kb on + strand at 101.961 kb on + strand at 101.962 kb on - strand at 102.052 kb on + strand, within Pf6N2E2_61 at 102.052 kb on + strand, within Pf6N2E2_61 at 102.052 kb on + strand, within Pf6N2E2_61 at 102.052 kb on + strand, within Pf6N2E2_61 at 102.052 kb on + strand, within Pf6N2E2_61 at 102.052 kb on + strand, within Pf6N2E2_61 at 102.052 kb on + strand, within Pf6N2E2_61 at 102.052 kb on + strand, within Pf6N2E2_61 at 102.053 kb on - strand, within Pf6N2E2_61 at 102.053 kb on - strand, within Pf6N2E2_61 at 102.053 kb on - strand, within Pf6N2E2_61 at 102.053 kb on - strand, within Pf6N2E2_61 at 102.053 kb on - strand, within Pf6N2E2_61 at 102.054 kb on + strand, within Pf6N2E2_61 at 102.054 kb on + strand, within Pf6N2E2_61 at 102.054 kb on + strand, within Pf6N2E2_61 at 102.055 kb on - strand, within Pf6N2E2_61 at 102.055 kb on - strand, within Pf6N2E2_61 at 102.055 kb on - strand, within Pf6N2E2_61 at 102.055 kb on - strand, within Pf6N2E2_61 at 102.059 kb on + strand, within Pf6N2E2_61 at 102.059 kb on + strand, within Pf6N2E2_61 at 102.060 kb on - strand, within Pf6N2E2_61 at 102.060 kb on - strand, within Pf6N2E2_61 at 102.077 kb on + strand, within Pf6N2E2_61 at 102.077 kb on + strand, within Pf6N2E2_61 at 102.077 kb on + strand, within Pf6N2E2_61 at 102.077 kb on + strand, within Pf6N2E2_61 at 102.078 kb on - strand, within Pf6N2E2_61 at 102.168 kb on - strand, within Pf6N2E2_61 at 102.180 kb on + strand, within Pf6N2E2_61 at 102.250 kb on + strand at 102.393 kb on - strand at 102.455 kb on + strand, within Pf6N2E2_62 at 102.550 kb on + strand, within Pf6N2E2_62 at 102.550 kb on + strand, within Pf6N2E2_62 at 102.551 kb on - strand, within Pf6N2E2_62 at 102.551 kb on - strand, within Pf6N2E2_62 at 102.551 kb on - strand, within Pf6N2E2_62 at 102.551 kb on - strand, within Pf6N2E2_62 at 102.823 kb on + strand at 102.824 kb on - strand at 102.824 kb on - strand at 102.824 kb on - strand at 102.824 kb on - strand at 102.825 kb on + strand at 102.825 kb on + strand at 102.825 kb on + strand at 102.825 kb on + strand at 102.826 kb on - strand at 102.826 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction tungstate 0.05 M remove 97,889 + +0.8 97,889 + -0.6 97,889 + -0.9 97,889 + +0.3 97,889 + -0.9 97,889 + +0.1 97,889 + +0.3 97,894 - +1.4 97,894 - +0.6 97,971 + -1.1 98,072 - Pf6N2E2_59 0.23 +0.7 98,072 - Pf6N2E2_59 0.23 -0.5 98,080 + Pf6N2E2_59 0.25 -0.1 98,080 + Pf6N2E2_59 0.25 +0.1 98,080 + Pf6N2E2_59 0.25 +0.8 98,080 + Pf6N2E2_59 0.25 +0.2 98,081 - Pf6N2E2_59 0.25 -0.6 98,227 + Pf6N2E2_59 0.58 -0.5 98,227 + Pf6N2E2_59 0.58 -1.2 98,228 - Pf6N2E2_59 0.58 +0.2 98,462 + +0.7 98,468 + -1.1 98,468 + +0.6 98,469 - -0.7 98,469 - +0.9 98,531 - +0.4 98,531 - -0.3 98,531 - -1.5 98,531 - -1.7 98,546 + -0.1 98,547 - +0.6 98,547 - -1.2 98,547 - +0.0 98,614 - -1.1 98,664 + +0.1 98,664 + -2.0 98,665 - -1.3 98,673 + -0.9 98,674 - +1.4 98,674 - -0.7 98,674 - +0.0 98,675 + +1.3 98,675 + -0.1 98,675 + +0.4 98,675 + -0.0 98,675 + +0.0 98,675 + -0.5 98,675 + -1.8 98,675 + -1.4 98,675 + -0.3 98,675 + -0.1 98,675 + +0.5 98,676 - +0.2 98,676 - -1.7 98,676 - +0.5 98,676 - -0.7 98,676 - +0.2 98,676 - -0.3 98,676 - +0.1 98,676 - +0.4 98,676 - -1.1 98,676 - +1.2 98,676 - +0.2 98,676 - -0.0 98,676 - -1.0 98,676 - +0.3 99,034 + Pf6N2E2_60 0.13 -1.3 99,035 - Pf6N2E2_60 0.13 -0.6 99,035 - Pf6N2E2_60 0.13 -0.4 99,035 - Pf6N2E2_60 0.13 -0.9 99,450 + Pf6N2E2_60 0.26 +0.2 99,516 - Pf6N2E2_60 0.28 -2.1 100,141 - Pf6N2E2_60 0.47 +0.2 100,414 + Pf6N2E2_60 0.55 -1.6 100,414 + Pf6N2E2_60 0.55 -1.4 100,414 + Pf6N2E2_60 0.55 +0.0 100,503 - Pf6N2E2_60 0.58 +0.0 100,776 + Pf6N2E2_60 0.66 +0.1 100,809 + Pf6N2E2_60 0.67 -0.6 100,809 + Pf6N2E2_60 0.67 +0.7 100,821 + Pf6N2E2_60 0.67 -0.4 100,821 + Pf6N2E2_60 0.67 -0.1 100,822 - Pf6N2E2_60 0.67 -0.1 100,822 - Pf6N2E2_60 0.67 -0.9 101,101 + Pf6N2E2_60 0.76 -0.4 101,101 + Pf6N2E2_60 0.76 -0.8 101,241 - Pf6N2E2_60 0.80 +0.4 101,331 + Pf6N2E2_60 0.83 -0.4 101,649 - -1.0 101,661 + +1.1 101,662 - +0.5 101,662 - -2.1 101,891 + -0.1 101,921 + -1.3 101,922 - +0.1 101,961 + -0.1 101,961 + -0.7 101,961 + +0.1 101,961 + -2.2 101,961 + -0.3 101,962 - +0.5 102,052 + Pf6N2E2_61 0.33 -0.2 102,052 + Pf6N2E2_61 0.33 +0.1 102,052 + Pf6N2E2_61 0.33 -0.6 102,052 + Pf6N2E2_61 0.33 -0.4 102,052 + Pf6N2E2_61 0.33 -1.2 102,052 + Pf6N2E2_61 0.33 -1.2 102,052 + Pf6N2E2_61 0.33 +1.2 102,052 + Pf6N2E2_61 0.33 +0.6 102,053 - Pf6N2E2_61 0.33 -1.1 102,053 - Pf6N2E2_61 0.33 +0.1 102,053 - Pf6N2E2_61 0.33 -0.3 102,053 - Pf6N2E2_61 0.33 -0.0 102,053 - Pf6N2E2_61 0.33 -1.1 102,054 + Pf6N2E2_61 0.34 -0.1 102,054 + Pf6N2E2_61 0.34 -1.6 102,054 + Pf6N2E2_61 0.34 -2.1 102,055 - Pf6N2E2_61 0.34 +1.4 102,055 - Pf6N2E2_61 0.34 +1.7 102,055 - Pf6N2E2_61 0.34 -1.6 102,055 - Pf6N2E2_61 0.34 -1.1 102,059 + Pf6N2E2_61 0.35 -1.1 102,059 + Pf6N2E2_61 0.35 -2.0 102,060 - Pf6N2E2_61 0.36 -0.1 102,060 - Pf6N2E2_61 0.36 -0.4 102,077 + Pf6N2E2_61 0.42 -1.7 102,077 + Pf6N2E2_61 0.42 -1.8 102,077 + Pf6N2E2_61 0.42 +1.4 102,077 + Pf6N2E2_61 0.42 -1.1 102,078 - Pf6N2E2_61 0.42 -0.0 102,168 - Pf6N2E2_61 0.75 +0.6 102,180 + Pf6N2E2_61 0.79 +0.5 102,250 + +0.4 102,393 - +0.4 102,455 + Pf6N2E2_62 0.38 +0.0 102,550 + Pf6N2E2_62 0.53 -1.8 102,550 + Pf6N2E2_62 0.53 -1.0 102,551 - Pf6N2E2_62 0.53 -1.1 102,551 - Pf6N2E2_62 0.53 -0.8 102,551 - Pf6N2E2_62 0.53 +0.4 102,551 - Pf6N2E2_62 0.53 +1.3 102,823 + -2.5 102,824 - -0.4 102,824 - -0.1 102,824 - +0.0 102,824 - +0.6 102,825 + +0.2 102,825 + -0.6 102,825 + -0.9 102,825 + -0.7 102,826 - +0.3 102,826 - +0.1
Or see this region's nucleotide sequence