Experiment: tungstate 0.05 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1127 and Pf6N2E2_1128 overlap by 10 nucleotides Pf6N2E2_1128 and Pf6N2E2_1129 overlap by 4 nucleotides Pf6N2E2_1129 and Pf6N2E2_1130 are separated by 173 nucleotides Pf6N2E2_1130 and Pf6N2E2_1131 are separated by 279 nucleotides
Pf6N2E2_1127 - Sensory box histidine kinase/response regulator, at 1,322,314 to 1,324,632
_1127
Pf6N2E2_1128 - Two-component response regulator, at 1,324,623 to 1,325,291
_1128
Pf6N2E2_1129 - Sensor histidine kinase, at 1,325,288 to 1,326,472
_1129
Pf6N2E2_1130 - hypothetical protein, at 1,326,646 to 1,327,167
_1130
Pf6N2E2_1131 - RNA polymerase sigma-70 factor, ECF subfamily, at 1,327,447 to 1,327,866
_1131
Position (kb)
1325
1326
1327 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1324.362 kb on - strand, within Pf6N2E2_1127 at 1324.362 kb on - strand, within Pf6N2E2_1127 at 1324.362 kb on - strand, within Pf6N2E2_1127 at 1324.397 kb on + strand, within Pf6N2E2_1127 at 1324.405 kb on + strand at 1324.405 kb on + strand at 1324.405 kb on + strand at 1324.406 kb on - strand at 1324.435 kb on - strand at 1324.516 kb on - strand at 1324.517 kb on + strand at 1324.517 kb on + strand at 1324.517 kb on + strand at 1324.518 kb on - strand at 1324.518 kb on - strand at 1324.518 kb on - strand at 1324.518 kb on - strand at 1324.518 kb on - strand at 1324.726 kb on - strand, within Pf6N2E2_1128 at 1324.982 kb on - strand, within Pf6N2E2_1128 at 1325.231 kb on + strand at 1325.688 kb on + strand, within Pf6N2E2_1129 at 1325.688 kb on + strand, within Pf6N2E2_1129 at 1325.708 kb on + strand, within Pf6N2E2_1129 at 1325.890 kb on - strand, within Pf6N2E2_1129 at 1326.101 kb on + strand, within Pf6N2E2_1129 at 1326.101 kb on + strand, within Pf6N2E2_1129 at 1326.102 kb on - strand, within Pf6N2E2_1129 at 1326.102 kb on - strand, within Pf6N2E2_1129 at 1326.102 kb on - strand, within Pf6N2E2_1129 at 1326.102 kb on - strand, within Pf6N2E2_1129 at 1326.522 kb on + strand at 1326.523 kb on - strand at 1326.548 kb on - strand at 1326.548 kb on - strand at 1326.678 kb on - strand at 1326.724 kb on + strand, within Pf6N2E2_1130 at 1326.724 kb on + strand, within Pf6N2E2_1130 at 1326.724 kb on + strand, within Pf6N2E2_1130 at 1326.725 kb on - strand, within Pf6N2E2_1130 at 1326.725 kb on - strand, within Pf6N2E2_1130 at 1326.725 kb on - strand, within Pf6N2E2_1130 at 1326.725 kb on - strand, within Pf6N2E2_1130 at 1326.730 kb on + strand, within Pf6N2E2_1130 at 1326.894 kb on + strand, within Pf6N2E2_1130 at 1326.894 kb on + strand, within Pf6N2E2_1130 at 1326.894 kb on + strand, within Pf6N2E2_1130 at 1326.894 kb on + strand, within Pf6N2E2_1130 at 1326.895 kb on - strand, within Pf6N2E2_1130 at 1326.917 kb on - strand, within Pf6N2E2_1130 at 1327.012 kb on + strand, within Pf6N2E2_1130 at 1327.013 kb on - strand, within Pf6N2E2_1130 at 1327.013 kb on - strand, within Pf6N2E2_1130 at 1327.013 kb on - strand, within Pf6N2E2_1130 at 1327.014 kb on + strand, within Pf6N2E2_1130 at 1327.014 kb on + strand, within Pf6N2E2_1130 at 1327.078 kb on + strand, within Pf6N2E2_1130 at 1327.078 kb on + strand, within Pf6N2E2_1130 at 1327.078 kb on + strand, within Pf6N2E2_1130 at 1327.079 kb on - strand, within Pf6N2E2_1130 at 1327.238 kb on + strand at 1327.255 kb on + strand at 1327.269 kb on + strand at 1327.269 kb on + strand at 1327.282 kb on + strand at 1327.282 kb on + strand at 1327.283 kb on - strand at 1327.283 kb on - strand at 1327.313 kb on - strand at 1327.402 kb on + strand at 1327.402 kb on + strand at 1327.416 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction tungstate 0.05 M remove 1,324,362 - Pf6N2E2_1127 0.88 -0.7 1,324,362 - Pf6N2E2_1127 0.88 +0.9 1,324,362 - Pf6N2E2_1127 0.88 +0.1 1,324,397 + Pf6N2E2_1127 0.90 +0.9 1,324,405 + -0.1 1,324,405 + -0.2 1,324,405 + -0.3 1,324,406 - +0.2 1,324,435 - -1.1 1,324,516 - +0.1 1,324,517 + -0.9 1,324,517 + -0.8 1,324,517 + -0.2 1,324,518 - +0.2 1,324,518 - -0.8 1,324,518 - +1.1 1,324,518 - +2.8 1,324,518 - +0.9 1,324,726 - Pf6N2E2_1128 0.15 -1.2 1,324,982 - Pf6N2E2_1128 0.54 +1.1 1,325,231 + +0.6 1,325,688 + Pf6N2E2_1129 0.34 -1.9 1,325,688 + Pf6N2E2_1129 0.34 -1.4 1,325,708 + Pf6N2E2_1129 0.35 +0.4 1,325,890 - Pf6N2E2_1129 0.51 -1.1 1,326,101 + Pf6N2E2_1129 0.69 -0.7 1,326,101 + Pf6N2E2_1129 0.69 -0.8 1,326,102 - Pf6N2E2_1129 0.69 -0.4 1,326,102 - Pf6N2E2_1129 0.69 -1.9 1,326,102 - Pf6N2E2_1129 0.69 -0.8 1,326,102 - Pf6N2E2_1129 0.69 -0.3 1,326,522 + -0.4 1,326,523 - +1.8 1,326,548 - -0.0 1,326,548 - -2.0 1,326,678 - -1.6 1,326,724 + Pf6N2E2_1130 0.15 +0.1 1,326,724 + Pf6N2E2_1130 0.15 +0.9 1,326,724 + Pf6N2E2_1130 0.15 +0.4 1,326,725 - Pf6N2E2_1130 0.15 -0.3 1,326,725 - Pf6N2E2_1130 0.15 -1.0 1,326,725 - Pf6N2E2_1130 0.15 +1.7 1,326,725 - Pf6N2E2_1130 0.15 +1.9 1,326,730 + Pf6N2E2_1130 0.16 +0.8 1,326,894 + Pf6N2E2_1130 0.48 +1.4 1,326,894 + Pf6N2E2_1130 0.48 +1.6 1,326,894 + Pf6N2E2_1130 0.48 -1.4 1,326,894 + Pf6N2E2_1130 0.48 -0.1 1,326,895 - Pf6N2E2_1130 0.48 +0.9 1,326,917 - Pf6N2E2_1130 0.52 -0.6 1,327,012 + Pf6N2E2_1130 0.70 -0.5 1,327,013 - Pf6N2E2_1130 0.70 +0.5 1,327,013 - Pf6N2E2_1130 0.70 -0.7 1,327,013 - Pf6N2E2_1130 0.70 -0.8 1,327,014 + Pf6N2E2_1130 0.70 -0.3 1,327,014 + Pf6N2E2_1130 0.70 +0.9 1,327,078 + Pf6N2E2_1130 0.83 +0.3 1,327,078 + Pf6N2E2_1130 0.83 +0.9 1,327,078 + Pf6N2E2_1130 0.83 -1.1 1,327,079 - Pf6N2E2_1130 0.83 +0.9 1,327,238 + +0.2 1,327,255 + -2.0 1,327,269 + +1.7 1,327,269 + +1.0 1,327,282 + -0.5 1,327,282 + -1.6 1,327,283 - +1.2 1,327,283 - -1.9 1,327,313 - +1.2 1,327,402 + -0.9 1,327,402 + +1.9 1,327,416 + +1.0
Or see this region's nucleotide sequence