Experiment: Formate McCas no stress control 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS00665 and MMP_RS00670 are separated by 33 nucleotides MMP_RS00670 and MMP_RS00675 are separated by 168 nucleotides MMP_RS00675 and MMP_RS00680 are separated by 132 nucleotides
MMP_RS00665: MMP_RS00665 - hypothetical protein, at 121,832 to 122,641
_RS00665
MMP_RS00670: MMP_RS00670 - TIM barrel protein, at 122,675 to 123,331
_RS00670
MMP_RS00675: MMP_RS00675 - nitroreductase family protein, at 123,500 to 124,021
_RS00675
MMP_RS00680: MMP_RS00680 - N-acetyl-gamma-glutamyl-phosphate reductase, at 124,154 to 125,185
_RS00680
Position (kb)
123
124
125 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 122.596 kb on - strand at 122.600 kb on - strand at 122.607 kb on + strand at 122.615 kb on + strand at 122.651 kb on - strand at 122.651 kb on - strand at 122.652 kb on + strand at 122.655 kb on - strand at 122.661 kb on + strand at 122.666 kb on - strand at 122.667 kb on + strand at 122.671 kb on - strand at 122.698 kb on - strand at 122.699 kb on + strand at 122.853 kb on - strand, within MMP_RS00670 at 122.857 kb on - strand, within MMP_RS00670 at 122.927 kb on - strand, within MMP_RS00670 at 122.972 kb on - strand, within MMP_RS00670 at 123.364 kb on - strand at 123.414 kb on - strand at 123.416 kb on - strand at 123.446 kb on - strand at 123.466 kb on - strand at 123.859 kb on + strand, within MMP_RS00675 at 124.055 kb on - strand at 124.094 kb on + strand at 124.109 kb on + strand at 124.339 kb on - strand at 124.480 kb on - strand at 124.484 kb on - strand at 124.485 kb on + strand at 124.873 kb on + strand at 124.907 kb on + strand at 124.907 kb on + strand at 124.908 kb on - strand at 124.908 kb on - strand at 124.908 kb on - strand at 124.909 kb on + strand at 124.946 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Formate McCas no stress control 2 remove 122,596 - -0.0 122,600 - -0.3 122,607 + -0.5 122,615 + +0.4 122,651 - +0.1 122,651 - -0.3 122,652 + +0.1 122,655 - -0.3 122,661 + +2.3 122,666 - -0.2 122,667 + -0.4 122,671 - +0.4 122,698 - +0.0 122,699 + -0.2 122,853 - MMP_RS00670 0.27 -0.1 122,857 - MMP_RS00670 0.28 -0.5 122,927 - MMP_RS00670 0.38 -0.1 122,972 - MMP_RS00670 0.45 +0.3 123,364 - +0.6 123,414 - +0.2 123,416 - -0.2 123,446 - -0.1 123,466 - -0.0 123,859 + MMP_RS00675 0.69 -0.6 124,055 - +0.3 124,094 + -0.6 124,109 + +0.6 124,339 - -0.6 124,480 - -1.2 124,484 - -0.6 124,485 + -0.7 124,873 + -0.2 124,907 + -1.5 124,907 + -0.8 124,908 - -2.8 124,908 - -1.5 124,908 - -1.4 124,909 + -1.5 124,946 - -0.3
Or see this region's nucleotide sequence