Experiment: Survival; Treatment=UGA synthetic gw pH4.4; TreatmentDuration=0.5hrs; Outgrowth=LBkan50
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt IAI47_09960 and IAI47_09965 are separated by 42 nucleotides IAI47_09965 and IAI47_09970 are separated by 83 nucleotides IAI47_09970 and IAI47_09975 overlap by 8 nucleotides
IAI47_09960: IAI47_09960 - NADH:flavin oxidoreductase/NADH oxidase, at 2,108,100 to 2,109,200
_09960
IAI47_09965: IAI47_09965 - NUDIX domain-containing protein, at 2,109,243 to 2,109,827
_09965
IAI47_09970: IAI47_09970 - DeoR/GlpR transcriptional regulator, at 2,109,911 to 2,110,660
_09970
IAI47_09975: IAI47_09975 - glutamate racemase, at 2,110,653 to 2,111,171
_09975
Position (kb)
2109
2110 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4
5 at 2108.261 kb on + strand, within IAI47_09960 at 2108.261 kb on + strand, within IAI47_09960 at 2108.294 kb on + strand, within IAI47_09960 at 2108.294 kb on + strand, within IAI47_09960 at 2108.295 kb on - strand, within IAI47_09960 at 2108.330 kb on + strand, within IAI47_09960 at 2108.330 kb on + strand, within IAI47_09960 at 2108.331 kb on - strand, within IAI47_09960 at 2108.331 kb on - strand, within IAI47_09960 at 2108.344 kb on + strand, within IAI47_09960 at 2108.344 kb on + strand, within IAI47_09960 at 2108.344 kb on + strand, within IAI47_09960 at 2108.393 kb on + strand, within IAI47_09960 at 2108.394 kb on - strand, within IAI47_09960 at 2108.417 kb on + strand, within IAI47_09960 at 2108.418 kb on - strand, within IAI47_09960 at 2108.488 kb on + strand, within IAI47_09960 at 2108.489 kb on - strand, within IAI47_09960 at 2108.489 kb on - strand, within IAI47_09960 at 2108.508 kb on + strand, within IAI47_09960 at 2108.508 kb on + strand, within IAI47_09960 at 2108.508 kb on + strand, within IAI47_09960 at 2108.509 kb on - strand, within IAI47_09960 at 2108.509 kb on - strand, within IAI47_09960 at 2108.509 kb on - strand, within IAI47_09960 at 2108.509 kb on - strand, within IAI47_09960 at 2108.526 kb on - strand, within IAI47_09960 at 2108.526 kb on - strand, within IAI47_09960 at 2108.526 kb on - strand, within IAI47_09960 at 2108.563 kb on + strand, within IAI47_09960 at 2108.609 kb on + strand, within IAI47_09960 at 2108.609 kb on + strand, within IAI47_09960 at 2108.694 kb on + strand, within IAI47_09960 at 2108.695 kb on - strand, within IAI47_09960 at 2108.695 kb on - strand, within IAI47_09960 at 2108.695 kb on - strand, within IAI47_09960 at 2108.877 kb on + strand, within IAI47_09960 at 2108.878 kb on - strand, within IAI47_09960 at 2108.878 kb on - strand, within IAI47_09960 at 2108.878 kb on - strand, within IAI47_09960 at 2108.879 kb on + strand, within IAI47_09960 at 2108.879 kb on + strand, within IAI47_09960 at 2108.879 kb on + strand, within IAI47_09960 at 2108.879 kb on + strand, within IAI47_09960 at 2108.937 kb on + strand, within IAI47_09960 at 2108.938 kb on - strand, within IAI47_09960 at 2108.961 kb on - strand, within IAI47_09960 at 2109.001 kb on + strand, within IAI47_09960 at 2109.002 kb on - strand, within IAI47_09960 at 2109.002 kb on - strand, within IAI47_09960 at 2109.002 kb on - strand, within IAI47_09960 at 2109.081 kb on + strand, within IAI47_09960 at 2109.081 kb on + strand, within IAI47_09960 at 2109.082 kb on - strand, within IAI47_09960 at 2109.147 kb on + strand at 2109.148 kb on - strand at 2109.148 kb on - strand at 2109.172 kb on + strand at 2109.172 kb on + strand at 2109.173 kb on - strand at 2109.173 kb on - strand at 2109.281 kb on + strand at 2109.281 kb on + strand at 2109.281 kb on + strand at 2109.281 kb on + strand at 2109.281 kb on + strand at 2109.281 kb on + strand at 2109.281 kb on + strand at 2109.281 kb on + strand at 2109.282 kb on - strand at 2109.282 kb on - strand at 2109.282 kb on - strand at 2109.282 kb on - strand at 2109.282 kb on - strand at 2109.282 kb on - strand at 2109.325 kb on - strand, within IAI47_09965 at 2109.325 kb on - strand, within IAI47_09965 at 2109.325 kb on - strand, within IAI47_09965 at 2109.352 kb on - strand, within IAI47_09965 at 2109.352 kb on - strand, within IAI47_09965 at 2109.360 kb on - strand, within IAI47_09965 at 2109.415 kb on + strand, within IAI47_09965 at 2109.426 kb on - strand, within IAI47_09965 at 2109.426 kb on - strand, within IAI47_09965 at 2109.426 kb on - strand, within IAI47_09965 at 2109.533 kb on + strand, within IAI47_09965 at 2109.533 kb on + strand, within IAI47_09965 at 2109.533 kb on + strand, within IAI47_09965 at 2109.533 kb on + strand, within IAI47_09965 at 2109.651 kb on - strand, within IAI47_09965 at 2109.660 kb on - strand, within IAI47_09965 at 2109.661 kb on + strand, within IAI47_09965 at 2109.661 kb on + strand, within IAI47_09965 at 2109.661 kb on + strand, within IAI47_09965 at 2109.661 kb on + strand, within IAI47_09965 at 2109.661 kb on + strand, within IAI47_09965 at 2109.661 kb on + strand, within IAI47_09965 at 2109.661 kb on + strand, within IAI47_09965 at 2109.662 kb on - strand, within IAI47_09965 at 2109.662 kb on - strand, within IAI47_09965 at 2109.662 kb on - strand, within IAI47_09965 at 2109.662 kb on - strand, within IAI47_09965 at 2109.662 kb on - strand, within IAI47_09965 at 2109.694 kb on + strand, within IAI47_09965 at 2109.694 kb on + strand, within IAI47_09965 at 2109.694 kb on + strand, within IAI47_09965 at 2109.749 kb on + strand, within IAI47_09965 at 2109.749 kb on + strand, within IAI47_09965 at 2109.749 kb on + strand, within IAI47_09965 at 2109.750 kb on - strand, within IAI47_09965 at 2109.750 kb on - strand, within IAI47_09965 at 2109.750 kb on - strand, within IAI47_09965 at 2109.751 kb on + strand, within IAI47_09965 at 2109.751 kb on + strand, within IAI47_09965 at 2109.752 kb on - strand, within IAI47_09965 at 2109.752 kb on - strand, within IAI47_09965 at 2109.752 kb on - strand, within IAI47_09965 at 2109.752 kb on - strand, within IAI47_09965 at 2109.752 kb on - strand, within IAI47_09965 at 2109.765 kb on - strand, within IAI47_09965 at 2109.766 kb on + strand, within IAI47_09965 at 2109.766 kb on + strand, within IAI47_09965 at 2109.767 kb on - strand, within IAI47_09965 at 2109.767 kb on - strand, within IAI47_09965 at 2109.773 kb on + strand at 2109.774 kb on - strand at 2109.774 kb on - strand at 2109.774 kb on - strand at 2109.863 kb on - strand at 2109.916 kb on - strand at 2109.969 kb on + strand at 2109.969 kb on + strand at 2109.970 kb on - strand at 2109.970 kb on - strand at 2110.053 kb on + strand, within IAI47_09970 at 2110.053 kb on + strand, within IAI47_09970 at 2110.054 kb on - strand, within IAI47_09970 at 2110.147 kb on + strand, within IAI47_09970 at 2110.195 kb on - strand, within IAI47_09970 at 2110.195 kb on - strand, within IAI47_09970 at 2110.227 kb on + strand, within IAI47_09970 at 2110.255 kb on + strand, within IAI47_09970 at 2110.320 kb on + strand, within IAI47_09970 at 2110.320 kb on + strand, within IAI47_09970 at 2110.320 kb on + strand, within IAI47_09970 at 2110.320 kb on + strand, within IAI47_09970 at 2110.321 kb on - strand, within IAI47_09970 at 2110.321 kb on - strand, within IAI47_09970 at 2110.321 kb on - strand, within IAI47_09970 at 2110.340 kb on + strand, within IAI47_09970 at 2110.340 kb on + strand, within IAI47_09970 at 2110.340 kb on + strand, within IAI47_09970 at 2110.340 kb on + strand, within IAI47_09970 at 2110.341 kb on - strand, within IAI47_09970 at 2110.341 kb on - strand, within IAI47_09970 at 2110.342 kb on + strand, within IAI47_09970 at 2110.342 kb on + strand, within IAI47_09970 at 2110.342 kb on + strand, within IAI47_09970 at 2110.372 kb on + strand, within IAI47_09970 at 2110.372 kb on + strand, within IAI47_09970 at 2110.372 kb on + strand, within IAI47_09970 at 2110.372 kb on + strand, within IAI47_09970 at 2110.372 kb on + strand, within IAI47_09970 at 2110.373 kb on - strand, within IAI47_09970 at 2110.373 kb on - strand, within IAI47_09970 at 2110.373 kb on - strand, within IAI47_09970 at 2110.396 kb on + strand, within IAI47_09970 at 2110.415 kb on - strand, within IAI47_09970 at 2110.585 kb on + strand, within IAI47_09970 at 2110.585 kb on + strand, within IAI47_09970 at 2110.687 kb on + strand at 2110.783 kb on + strand, within IAI47_09975 at 2110.784 kb on - strand, within IAI47_09975 at 2110.784 kb on - strand, within IAI47_09975 at 2110.819 kb on + strand, within IAI47_09975 at 2110.819 kb on + strand, within IAI47_09975 at 2110.819 kb on + strand, within IAI47_09975 at 2110.819 kb on + strand, within IAI47_09975
Per-strain Table
Position Strand Gene LocusTag Fraction Survival; Treatment=UGA synthetic gw pH4.4; TreatmentDuration=0.5hrs; Outgrowth=LBkan50 remove 2,108,261 + IAI47_09960 0.15 -1.8 2,108,261 + IAI47_09960 0.15 +1.6 2,108,294 + IAI47_09960 0.18 -1.2 2,108,294 + IAI47_09960 0.18 -0.9 2,108,295 - IAI47_09960 0.18 +1.4 2,108,330 + IAI47_09960 0.21 -0.2 2,108,330 + IAI47_09960 0.21 -0.9 2,108,331 - IAI47_09960 0.21 +0.8 2,108,331 - IAI47_09960 0.21 +1.6 2,108,344 + IAI47_09960 0.22 -1.9 2,108,344 + IAI47_09960 0.22 -0.2 2,108,344 + IAI47_09960 0.22 +0.2 2,108,393 + IAI47_09960 0.27 -0.6 2,108,394 - IAI47_09960 0.27 +0.4 2,108,417 + IAI47_09960 0.29 +0.8 2,108,418 - IAI47_09960 0.29 +1.1 2,108,488 + IAI47_09960 0.35 -0.7 2,108,489 - IAI47_09960 0.35 -2.1 2,108,489 - IAI47_09960 0.35 -1.4 2,108,508 + IAI47_09960 0.37 -0.4 2,108,508 + IAI47_09960 0.37 -0.7 2,108,508 + IAI47_09960 0.37 +0.1 2,108,509 - IAI47_09960 0.37 -2.3 2,108,509 - IAI47_09960 0.37 +0.5 2,108,509 - IAI47_09960 0.37 +0.1 2,108,509 - IAI47_09960 0.37 -2.1 2,108,526 - IAI47_09960 0.39 +1.4 2,108,526 - IAI47_09960 0.39 +3.4 2,108,526 - IAI47_09960 0.39 +1.5 2,108,563 + IAI47_09960 0.42 +2.7 2,108,609 + IAI47_09960 0.46 -1.4 2,108,609 + IAI47_09960 0.46 +2.0 2,108,694 + IAI47_09960 0.54 +0.1 2,108,695 - IAI47_09960 0.54 +4.8 2,108,695 - IAI47_09960 0.54 +1.5 2,108,695 - IAI47_09960 0.54 +0.7 2,108,877 + IAI47_09960 0.71 +0.9 2,108,878 - IAI47_09960 0.71 +1.1 2,108,878 - IAI47_09960 0.71 -1.8 2,108,878 - IAI47_09960 0.71 -3.1 2,108,879 + IAI47_09960 0.71 +0.4 2,108,879 + IAI47_09960 0.71 +2.4 2,108,879 + IAI47_09960 0.71 +0.3 2,108,879 + IAI47_09960 0.71 +0.9 2,108,937 + IAI47_09960 0.76 -2.1 2,108,938 - IAI47_09960 0.76 -0.9 2,108,961 - IAI47_09960 0.78 -0.1 2,109,001 + IAI47_09960 0.82 -0.4 2,109,002 - IAI47_09960 0.82 -0.6 2,109,002 - IAI47_09960 0.82 +1.4 2,109,002 - IAI47_09960 0.82 -1.4 2,109,081 + IAI47_09960 0.89 +0.8 2,109,081 + IAI47_09960 0.89 -0.2 2,109,082 - IAI47_09960 0.89 +0.4 2,109,147 + +2.4 2,109,148 - -2.4 2,109,148 - +1.6 2,109,172 + +0.5 2,109,172 + +0.4 2,109,173 - -0.8 2,109,173 - +0.2 2,109,281 + -2.5 2,109,281 + +1.1 2,109,281 + -0.8 2,109,281 + +0.7 2,109,281 + -0.9 2,109,281 + -1.3 2,109,281 + +1.2 2,109,281 + -1.4 2,109,282 - +0.2 2,109,282 - -0.2 2,109,282 - -0.9 2,109,282 - +0.2 2,109,282 - -1.4 2,109,282 - -1.6 2,109,325 - IAI47_09965 0.14 -0.9 2,109,325 - IAI47_09965 0.14 -0.2 2,109,325 - IAI47_09965 0.14 -1.2 2,109,352 - IAI47_09965 0.19 -2.2 2,109,352 - IAI47_09965 0.19 +0.8 2,109,360 - IAI47_09965 0.20 +1.4 2,109,415 + IAI47_09965 0.29 -1.4 2,109,426 - IAI47_09965 0.31 +0.2 2,109,426 - IAI47_09965 0.31 +1.4 2,109,426 - IAI47_09965 0.31 -1.9 2,109,533 + IAI47_09965 0.50 +0.4 2,109,533 + IAI47_09965 0.50 +1.4 2,109,533 + IAI47_09965 0.50 +1.8 2,109,533 + IAI47_09965 0.50 +0.6 2,109,651 - IAI47_09965 0.70 +0.5 2,109,660 - IAI47_09965 0.71 -0.8 2,109,661 + IAI47_09965 0.71 +2.4 2,109,661 + IAI47_09965 0.71 +0.3 2,109,661 + IAI47_09965 0.71 -0.7 2,109,661 + IAI47_09965 0.71 -2.0 2,109,661 + IAI47_09965 0.71 -0.8 2,109,661 + IAI47_09965 0.71 +5.2 2,109,661 + IAI47_09965 0.71 -0.2 2,109,662 - IAI47_09965 0.72 -0.2 2,109,662 - IAI47_09965 0.72 +3.7 2,109,662 - IAI47_09965 0.72 +1.4 2,109,662 - IAI47_09965 0.72 +1.4 2,109,662 - IAI47_09965 0.72 +1.0 2,109,694 + IAI47_09965 0.77 +0.2 2,109,694 + IAI47_09965 0.77 +1.4 2,109,694 + IAI47_09965 0.77 -0.2 2,109,749 + IAI47_09965 0.86 -1.5 2,109,749 + IAI47_09965 0.86 -1.1 2,109,749 + IAI47_09965 0.86 +0.7 2,109,750 - IAI47_09965 0.87 +3.7 2,109,750 - IAI47_09965 0.87 -0.6 2,109,750 - IAI47_09965 0.87 +2.4 2,109,751 + IAI47_09965 0.87 -1.2 2,109,751 + IAI47_09965 0.87 -2.2 2,109,752 - IAI47_09965 0.87 +1.8 2,109,752 - IAI47_09965 0.87 +0.6 2,109,752 - IAI47_09965 0.87 +1.0 2,109,752 - IAI47_09965 0.87 -0.1 2,109,752 - IAI47_09965 0.87 -1.1 2,109,765 - IAI47_09965 0.89 -0.4 2,109,766 + IAI47_09965 0.89 +1.5 2,109,766 + IAI47_09965 0.89 +2.0 2,109,767 - IAI47_09965 0.90 -1.8 2,109,767 - IAI47_09965 0.90 +0.4 2,109,773 + +0.5 2,109,774 - -1.6 2,109,774 - +0.4 2,109,774 - -2.4 2,109,863 - -0.2 2,109,916 - +2.5 2,109,969 + +0.2 2,109,969 + -0.9 2,109,970 - -0.9 2,109,970 - -2.9 2,110,053 + IAI47_09970 0.19 -0.1 2,110,053 + IAI47_09970 0.19 -1.6 2,110,054 - IAI47_09970 0.19 +1.0 2,110,147 + IAI47_09970 0.31 -0.9 2,110,195 - IAI47_09970 0.38 +3.2 2,110,195 - IAI47_09970 0.38 +2.0 2,110,227 + IAI47_09970 0.42 -0.6 2,110,255 + IAI47_09970 0.46 +1.1 2,110,320 + IAI47_09970 0.55 +2.4 2,110,320 + IAI47_09970 0.55 -0.3 2,110,320 + IAI47_09970 0.55 -2.6 2,110,320 + IAI47_09970 0.55 -1.9 2,110,321 - IAI47_09970 0.55 -2.1 2,110,321 - IAI47_09970 0.55 +1.4 2,110,321 - IAI47_09970 0.55 +1.9 2,110,340 + IAI47_09970 0.57 -0.9 2,110,340 + IAI47_09970 0.57 +0.5 2,110,340 + IAI47_09970 0.57 +1.1 2,110,340 + IAI47_09970 0.57 -2.2 2,110,341 - IAI47_09970 0.57 +1.0 2,110,341 - IAI47_09970 0.57 +0.7 2,110,342 + IAI47_09970 0.57 -1.6 2,110,342 + IAI47_09970 0.57 +1.1 2,110,342 + IAI47_09970 0.57 -1.2 2,110,372 + IAI47_09970 0.61 +2.8 2,110,372 + IAI47_09970 0.61 +1.1 2,110,372 + IAI47_09970 0.61 +0.7 2,110,372 + IAI47_09970 0.61 -1.1 2,110,372 + IAI47_09970 0.61 -0.2 2,110,373 - IAI47_09970 0.62 -0.2 2,110,373 - IAI47_09970 0.62 +0.2 2,110,373 - IAI47_09970 0.62 +1.9 2,110,396 + IAI47_09970 0.65 -0.4 2,110,415 - IAI47_09970 0.67 +0.1 2,110,585 + IAI47_09970 0.90 -1.9 2,110,585 + IAI47_09970 0.90 -1.3 2,110,687 + -1.2 2,110,783 + IAI47_09975 0.25 +0.2 2,110,784 - IAI47_09975 0.25 +1.1 2,110,784 - IAI47_09975 0.25 +0.3 2,110,819 + IAI47_09975 0.32 +0.5 2,110,819 + IAI47_09975 0.32 -1.8 2,110,819 + IAI47_09975 0.32 +0.1 2,110,819 + IAI47_09975 0.32 -1.2
Or see this region's nucleotide sequence