Experiment: m-Inositol (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS01180 and BWI76_RS01185 are separated by 346 nucleotides BWI76_RS01185 and BWI76_RS01190 are separated by 80 nucleotides BWI76_RS01190 and BWI76_RS01195 are separated by 138 nucleotides BWI76_RS01195 and BWI76_RS01200 are separated by 25 nucleotides
BWI76_RS01180: BWI76_RS01180 - porphobilinogen deaminase, at 240,754 to 241,695
_RS01180
BWI76_RS01185: BWI76_RS01185 - adenylate cyclase, at 242,042 to 244,591
_RS01185
BWI76_RS01190: BWI76_RS01190 - iron donor protein CyaY, at 244,672 to 244,992
_RS01190
BWI76_RS01195: BWI76_RS01195 - membrane protein, at 245,131 to 245,334
_RS01195
BWI76_RS01200: BWI76_RS01200 - diaminopimelate epimerase, at 245,360 to 246,184
_RS01200
Position (kb) 242
243
244
245 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 241.724 kb on - strand at 241.765 kb on + strand at 241.857 kb on - strand at 241.938 kb on + strand at 242.047 kb on + strand at 242.055 kb on - strand at 243.014 kb on - strand, within BWI76_RS01185 at 243.445 kb on - strand, within BWI76_RS01185 at 243.456 kb on + strand, within BWI76_RS01185 at 243.956 kb on - strand, within BWI76_RS01185 at 243.956 kb on - strand, within BWI76_RS01185 at 244.498 kb on + strand at 244.555 kb on - strand at 244.608 kb on + strand at 244.876 kb on - strand, within BWI76_RS01190 at 244.920 kb on - strand, within BWI76_RS01190 at 245.186 kb on + strand, within BWI76_RS01195 at 245.194 kb on - strand, within BWI76_RS01195 at 245.337 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction m-Inositol (C) remove 241,724 - -0.3 241,765 + +0.6 241,857 - -0.6 241,938 + +0.6 242,047 + -3.1 242,055 - -1.4 243,014 - BWI76_RS01185 0.38 -0.4 243,445 - BWI76_RS01185 0.55 -2.0 243,456 + BWI76_RS01185 0.55 -2.2 243,956 - BWI76_RS01185 0.75 -2.0 243,956 - BWI76_RS01185 0.75 +1.6 244,498 + -0.7 244,555 - +1.0 244,608 + +0.4 244,876 - BWI76_RS01190 0.64 +0.5 244,920 - BWI76_RS01190 0.77 +0.1 245,186 + BWI76_RS01195 0.27 +0.2 245,194 - BWI76_RS01195 0.31 -1.2 245,337 + +1.1
Or see this region's nucleotide sequence