Experiment: Recovery broth with Cerulenin 0.9 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS13195 and BBR_RS13200 are separated by 26 nucleotides BBR_RS13200 and BBR_RS13205 are separated by 57 nucleotides
BBR_RS13195: BBR_RS13195 - pyridoxal 5'-phosphate synthase lyase subunit PdxS, at 736,662 to 737,537
_RS13195
BBR_RS13200: BBR_RS13200 - pyridoxal 5'-phosphate synthase glutaminase subunit PdxT, at 737,564 to 738,202
_RS13200
BBR_RS13205: BBR_RS13205 - PLP-dependent aminotransferase family protein, at 738,260 to 739,783
_RS13205
Position (kb)
737
738
739 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 736.587 kb on + strand at 736.587 kb on + strand at 736.587 kb on + strand at 736.595 kb on - strand at 736.598 kb on + strand at 736.600 kb on - strand at 736.632 kb on + strand at 736.638 kb on - strand at 736.640 kb on + strand at 736.647 kb on + strand at 736.788 kb on - strand, within BBR_RS13195 at 736.851 kb on + strand, within BBR_RS13195 at 736.856 kb on + strand, within BBR_RS13195 at 736.864 kb on - strand, within BBR_RS13195 at 737.001 kb on - strand, within BBR_RS13195 at 737.005 kb on - strand, within BBR_RS13195 at 737.049 kb on + strand, within BBR_RS13195 at 737.058 kb on - strand, within BBR_RS13195 at 737.062 kb on - strand, within BBR_RS13195 at 737.088 kb on + strand, within BBR_RS13195 at 737.137 kb on - strand, within BBR_RS13195 at 737.212 kb on + strand, within BBR_RS13195 at 737.233 kb on - strand, within BBR_RS13195 at 737.239 kb on - strand, within BBR_RS13195 at 737.277 kb on + strand, within BBR_RS13195 at 737.298 kb on + strand, within BBR_RS13195 at 737.335 kb on - strand, within BBR_RS13195 at 737.361 kb on + strand, within BBR_RS13195 at 737.370 kb on + strand, within BBR_RS13195 at 737.437 kb on + strand, within BBR_RS13195 at 737.437 kb on + strand, within BBR_RS13195 at 737.445 kb on - strand, within BBR_RS13195 at 737.513 kb on + strand at 737.547 kb on + strand at 737.557 kb on + strand at 737.560 kb on - strand at 737.584 kb on - strand at 737.616 kb on - strand at 737.642 kb on - strand, within BBR_RS13200 at 737.653 kb on - strand, within BBR_RS13200 at 737.830 kb on + strand, within BBR_RS13200 at 737.830 kb on + strand, within BBR_RS13200 at 737.830 kb on + strand, within BBR_RS13200 at 737.830 kb on + strand, within BBR_RS13200 at 737.830 kb on + strand, within BBR_RS13200 at 737.830 kb on + strand, within BBR_RS13200 at 737.838 kb on - strand, within BBR_RS13200 at 737.928 kb on + strand, within BBR_RS13200 at 737.931 kb on + strand, within BBR_RS13200 at 737.948 kb on + strand, within BBR_RS13200 at 737.948 kb on + strand, within BBR_RS13200 at 737.955 kb on + strand, within BBR_RS13200 at 737.955 kb on + strand, within BBR_RS13200 at 737.955 kb on - strand, within BBR_RS13200 at 737.963 kb on - strand, within BBR_RS13200 at 737.963 kb on - strand, within BBR_RS13200 at 738.006 kb on + strand, within BBR_RS13200 at 738.033 kb on - strand, within BBR_RS13200 at 738.035 kb on + strand, within BBR_RS13200 at 738.035 kb on + strand, within BBR_RS13200 at 738.077 kb on - strand, within BBR_RS13200 at 738.091 kb on - strand, within BBR_RS13200 at 738.091 kb on - strand, within BBR_RS13200 at 738.131 kb on + strand, within BBR_RS13200 at 738.223 kb on - strand at 738.223 kb on - strand at 738.226 kb on + strand at 738.255 kb on + strand at 738.278 kb on - strand at 738.288 kb on - strand at 738.288 kb on - strand at 738.293 kb on - strand at 738.296 kb on - strand at 738.312 kb on + strand at 738.312 kb on + strand at 738.381 kb on + strand at 738.401 kb on - strand at 738.409 kb on - strand at 738.422 kb on - strand, within BBR_RS13205 at 738.424 kb on - strand, within BBR_RS13205 at 738.431 kb on - strand, within BBR_RS13205 at 738.517 kb on + strand, within BBR_RS13205 at 738.520 kb on - strand, within BBR_RS13205 at 738.539 kb on - strand, within BBR_RS13205 at 738.587 kb on + strand, within BBR_RS13205 at 738.595 kb on - strand, within BBR_RS13205 at 738.649 kb on - strand, within BBR_RS13205 at 738.740 kb on - strand, within BBR_RS13205 at 738.740 kb on - strand, within BBR_RS13205 at 738.828 kb on - strand, within BBR_RS13205 at 738.836 kb on + strand, within BBR_RS13205 at 738.860 kb on - strand, within BBR_RS13205 at 738.879 kb on - strand, within BBR_RS13205 at 738.879 kb on - strand, within BBR_RS13205 at 738.937 kb on - strand, within BBR_RS13205 at 739.024 kb on - strand, within BBR_RS13205 at 739.075 kb on + strand, within BBR_RS13205 at 739.083 kb on - strand, within BBR_RS13205 at 739.083 kb on - strand, within BBR_RS13205 at 739.085 kb on + strand, within BBR_RS13205 at 739.093 kb on - strand, within BBR_RS13205 at 739.093 kb on - strand, within BBR_RS13205 at 739.176 kb on - strand, within BBR_RS13205
Per-strain Table
Position Strand Gene LocusTag Fraction Recovery broth with Cerulenin 0.9 mM remove 736,587 + +0.3 736,587 + +1.0 736,587 + -0.3 736,595 - -0.1 736,598 + +1.7 736,600 - -1.3 736,632 + +0.8 736,638 - +1.0 736,640 + -3.2 736,647 + +0.8 736,788 - BBR_RS13195 0.14 -1.2 736,851 + BBR_RS13195 0.22 -0.1 736,856 + BBR_RS13195 0.22 +0.3 736,864 - BBR_RS13195 0.23 +0.4 737,001 - BBR_RS13195 0.39 +1.3 737,005 - BBR_RS13195 0.39 +0.3 737,049 + BBR_RS13195 0.44 +0.7 737,058 - BBR_RS13195 0.45 -0.8 737,062 - BBR_RS13195 0.46 +0.7 737,088 + BBR_RS13195 0.49 +0.7 737,137 - BBR_RS13195 0.54 +0.8 737,212 + BBR_RS13195 0.63 +1.0 737,233 - BBR_RS13195 0.65 +0.2 737,239 - BBR_RS13195 0.66 +0.1 737,277 + BBR_RS13195 0.70 +0.1 737,298 + BBR_RS13195 0.73 +0.3 737,335 - BBR_RS13195 0.77 -0.1 737,361 + BBR_RS13195 0.80 +1.0 737,370 + BBR_RS13195 0.81 +0.5 737,437 + BBR_RS13195 0.88 +1.3 737,437 + BBR_RS13195 0.88 +1.6 737,445 - BBR_RS13195 0.89 -0.9 737,513 + +0.4 737,547 + +0.1 737,557 + -0.1 737,560 - -0.3 737,584 - +0.9 737,616 - +0.7 737,642 - BBR_RS13200 0.12 +0.3 737,653 - BBR_RS13200 0.14 +0.2 737,830 + BBR_RS13200 0.42 +0.2 737,830 + BBR_RS13200 0.42 +0.1 737,830 + BBR_RS13200 0.42 +1.0 737,830 + BBR_RS13200 0.42 +0.4 737,830 + BBR_RS13200 0.42 +0.5 737,830 + BBR_RS13200 0.42 +0.9 737,838 - BBR_RS13200 0.43 +0.9 737,928 + BBR_RS13200 0.57 +1.6 737,931 + BBR_RS13200 0.57 +0.5 737,948 + BBR_RS13200 0.60 +2.4 737,948 + BBR_RS13200 0.60 +0.6 737,955 + BBR_RS13200 0.61 +0.7 737,955 + BBR_RS13200 0.61 +0.8 737,955 - BBR_RS13200 0.61 +0.2 737,963 - BBR_RS13200 0.62 +0.6 737,963 - BBR_RS13200 0.62 -0.0 738,006 + BBR_RS13200 0.69 -0.1 738,033 - BBR_RS13200 0.73 +1.1 738,035 + BBR_RS13200 0.74 +0.1 738,035 + BBR_RS13200 0.74 +0.6 738,077 - BBR_RS13200 0.80 -0.2 738,091 - BBR_RS13200 0.82 +0.6 738,091 - BBR_RS13200 0.82 -0.0 738,131 + BBR_RS13200 0.89 +0.5 738,223 - +0.2 738,223 - +0.8 738,226 + +1.0 738,255 + -0.4 738,278 - +0.3 738,288 - +0.8 738,288 - +0.3 738,293 - -0.0 738,296 - +1.0 738,312 + -0.7 738,312 + +0.3 738,381 + -0.2 738,401 - +0.2 738,409 - -0.3 738,422 - BBR_RS13205 0.11 +0.7 738,424 - BBR_RS13205 0.11 +1.2 738,431 - BBR_RS13205 0.11 +1.3 738,517 + BBR_RS13205 0.17 +0.4 738,520 - BBR_RS13205 0.17 -1.0 738,539 - BBR_RS13205 0.18 +0.1 738,587 + BBR_RS13205 0.21 -0.1 738,595 - BBR_RS13205 0.22 -0.2 738,649 - BBR_RS13205 0.26 +0.2 738,740 - BBR_RS13205 0.31 +0.4 738,740 - BBR_RS13205 0.31 +0.3 738,828 - BBR_RS13205 0.37 +0.0 738,836 + BBR_RS13205 0.38 +0.2 738,860 - BBR_RS13205 0.39 +0.5 738,879 - BBR_RS13205 0.41 +0.5 738,879 - BBR_RS13205 0.41 +0.5 738,937 - BBR_RS13205 0.44 +0.2 739,024 - BBR_RS13205 0.50 +0.4 739,075 + BBR_RS13205 0.53 +0.1 739,083 - BBR_RS13205 0.54 +0.5 739,083 - BBR_RS13205 0.54 -0.3 739,085 + BBR_RS13205 0.54 +1.6 739,093 - BBR_RS13205 0.55 +0.4 739,093 - BBR_RS13205 0.55 -0.2 739,176 - BBR_RS13205 0.60 +0.0
Or see this region's nucleotide sequence