Experiment: MoYLS4 with BPS 20uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt fliI and DVU0311 overlap by 4 nucleotides DVU0311 and fliG overlap by 14 nucleotides fliG and fliF overlap by 32 nucleotides
DVU0310: fliI - flagellum-specific ATP synthase FliI (TIGR), at 354,912 to 356,225
fliI
DVU0311: DVU0311 - flagellar assembly protein FliH, putative (TIGR), at 356,222 to 356,974
DVU0311
DVU0312: fliG - flagellar motor switch protein FliG (TIGR), at 356,961 to 357,977
fliG
DVU0313: fliF - flagellar M-ring protein FliF (TIGR), at 357,946 to 359,562
fliF
Position (kb) 356
357
358 Strain fitness (log2 ratio) -2
-1
0
1
2 at 356.000 kb on + strand, within fliI at 356.008 kb on - strand, within fliI at 356.008 kb on - strand, within fliI at 356.008 kb on - strand, within fliI at 356.012 kb on - strand, within fliI at 356.013 kb on - strand, within fliI at 356.084 kb on + strand, within fliI at 356.085 kb on + strand, within fliI at 356.145 kb on - strand at 356.147 kb on - strand at 356.154 kb on - strand at 356.204 kb on - strand at 356.249 kb on - strand at 356.263 kb on - strand at 356.277 kb on - strand at 356.278 kb on - strand at 356.426 kb on + strand, within DVU0311 at 356.495 kb on + strand, within DVU0311 at 356.516 kb on + strand, within DVU0311 at 356.646 kb on + strand, within DVU0311 at 356.741 kb on - strand, within DVU0311 at 356.797 kb on + strand, within DVU0311 at 356.883 kb on + strand, within DVU0311 at 356.898 kb on - strand, within DVU0311 at 356.926 kb on - strand at 356.927 kb on + strand at 356.936 kb on - strand at 357.003 kb on + strand at 357.003 kb on + strand at 357.045 kb on - strand at 357.047 kb on - strand at 357.060 kb on - strand at 357.069 kb on - strand, within fliG at 357.181 kb on - strand, within fliG at 357.202 kb on + strand, within fliG at 357.202 kb on + strand, within fliG at 357.210 kb on - strand, within fliG at 357.366 kb on + strand, within fliG at 357.417 kb on - strand, within fliG at 357.466 kb on + strand, within fliG at 357.466 kb on + strand, within fliG at 357.601 kb on + strand, within fliG at 357.652 kb on + strand, within fliG at 357.664 kb on + strand, within fliG at 357.682 kb on - strand, within fliG at 357.710 kb on - strand, within fliG at 357.763 kb on - strand, within fliG at 357.799 kb on + strand, within fliG at 357.802 kb on - strand, within fliG at 357.802 kb on - strand, within fliG at 357.802 kb on - strand, within fliG at 357.864 kb on - strand, within fliG at 357.885 kb on + strand at 357.943 kb on + strand at 357.943 kb on + strand at 357.943 kb on - strand at 357.951 kb on - strand at 358.015 kb on - strand at 358.033 kb on + strand at 358.046 kb on + strand at 358.054 kb on - strand at 358.090 kb on - strand at 358.148 kb on - strand, within fliF at 358.188 kb on - strand, within fliF at 358.202 kb on - strand, within fliF at 358.238 kb on + strand, within fliF at 358.238 kb on + strand, within fliF at 358.274 kb on - strand, within fliF at 358.334 kb on + strand, within fliF at 358.341 kb on + strand, within fliF at 358.374 kb on - strand, within fliF at 358.435 kb on + strand, within fliF at 358.443 kb on - strand, within fliF at 358.443 kb on - strand, within fliF at 358.458 kb on + strand, within fliF at 358.504 kb on - strand, within fliF at 358.533 kb on - strand, within fliF at 358.621 kb on - strand, within fliF at 358.640 kb on + strand, within fliF at 358.683 kb on + strand, within fliF at 358.691 kb on - strand, within fliF at 358.765 kb on - strand, within fliF at 358.772 kb on - strand, within fliF at 358.796 kb on + strand, within fliF at 358.796 kb on + strand, within fliF at 358.804 kb on - strand, within fliF at 358.804 kb on - strand, within fliF at 358.967 kb on - strand, within fliF
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with BPS 20uM remove 356,000 + fliI DVU0310 0.83 -0.5 356,008 - fliI DVU0310 0.83 -0.7 356,008 - fliI DVU0310 0.83 -0.1 356,008 - fliI DVU0310 0.83 -2.2 356,012 - fliI DVU0310 0.84 -0.5 356,013 - fliI DVU0310 0.84 -0.4 356,084 + fliI DVU0310 0.89 -0.3 356,085 + fliI DVU0310 0.89 -0.6 356,145 - +0.5 356,147 - -1.4 356,154 - -0.1 356,204 - -0.4 356,249 - -0.3 356,263 - +0.5 356,277 - -1.6 356,278 - -0.5 356,426 + DVU0311 0.27 -0.5 356,495 + DVU0311 0.36 -0.5 356,516 + DVU0311 0.39 -0.6 356,646 + DVU0311 0.56 +1.1 356,741 - DVU0311 0.69 -0.2 356,797 + DVU0311 0.76 -0.6 356,883 + DVU0311 0.88 +0.0 356,898 - DVU0311 0.90 -0.6 356,926 - -1.1 356,927 + +1.3 356,936 - -0.2 357,003 + -0.5 357,003 + -0.3 357,045 - -0.5 357,047 - -0.4 357,060 - -0.4 357,069 - fliG DVU0312 0.11 -0.3 357,181 - fliG DVU0312 0.22 -0.8 357,202 + fliG DVU0312 0.24 -0.9 357,202 + fliG DVU0312 0.24 -0.5 357,210 - fliG DVU0312 0.24 -1.4 357,366 + fliG DVU0312 0.40 -0.3 357,417 - fliG DVU0312 0.45 -0.4 357,466 + fliG DVU0312 0.50 -0.7 357,466 + fliG DVU0312 0.50 -0.5 357,601 + fliG DVU0312 0.63 -0.5 357,652 + fliG DVU0312 0.68 -0.7 357,664 + fliG DVU0312 0.69 -0.4 357,682 - fliG DVU0312 0.71 -0.4 357,710 - fliG DVU0312 0.74 +0.0 357,763 - fliG DVU0312 0.79 -0.3 357,799 + fliG DVU0312 0.82 -0.4 357,802 - fliG DVU0312 0.83 +0.0 357,802 - fliG DVU0312 0.83 -0.7 357,802 - fliG DVU0312 0.83 +0.5 357,864 - fliG DVU0312 0.89 -0.8 357,885 + +0.4 357,943 + +2.3 357,943 + -0.7 357,943 - -0.4 357,951 - -0.2 358,015 - -0.4 358,033 + -0.3 358,046 + -0.3 358,054 - -0.6 358,090 - -1.0 358,148 - fliF DVU0313 0.12 -0.2 358,188 - fliF DVU0313 0.15 -0.8 358,202 - fliF DVU0313 0.16 -0.2 358,238 + fliF DVU0313 0.18 -0.5 358,238 + fliF DVU0313 0.18 -0.5 358,274 - fliF DVU0313 0.20 +0.4 358,334 + fliF DVU0313 0.24 +0.5 358,341 + fliF DVU0313 0.24 -0.3 358,374 - fliF DVU0313 0.26 -0.9 358,435 + fliF DVU0313 0.30 -0.6 358,443 - fliF DVU0313 0.31 -0.4 358,443 - fliF DVU0313 0.31 -0.5 358,458 + fliF DVU0313 0.32 -0.9 358,504 - fliF DVU0313 0.35 -0.6 358,533 - fliF DVU0313 0.36 -0.6 358,621 - fliF DVU0313 0.42 -1.6 358,640 + fliF DVU0313 0.43 -0.5 358,683 + fliF DVU0313 0.46 -0.4 358,691 - fliF DVU0313 0.46 -0.6 358,765 - fliF DVU0313 0.51 -0.7 358,772 - fliF DVU0313 0.51 -0.2 358,796 + fliF DVU0313 0.53 -0.5 358,796 + fliF DVU0313 0.53 -0.5 358,804 - fliF DVU0313 0.53 -1.3 358,804 - fliF DVU0313 0.53 -0.5 358,967 - fliF DVU0313 0.63 -0.7
Or see this region's nucleotide sequence