Experiment: Fluorophosphate 0.125 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_949 and Pf6N2E2_950 are separated by 516 nucleotides Pf6N2E2_950 and Pf6N2E2_951 overlap by 1 nucleotides Pf6N2E2_951 and Pf6N2E2_952 are separated by 70 nucleotides
Pf6N2E2_949 - Outer membrane protein OprN, at 1,118,637 to 1,120,055
_949
Pf6N2E2_950 - Type IV secretory pathway, VirB4 components, at 1,120,572 to 1,123,322
_950
Pf6N2E2_951 - putative lipoprotein, at 1,123,322 to 1,123,630
_951
Pf6N2E2_952 - putative exported protein, at 1,123,701 to 1,125,215
_952
Position (kb)
1120
1121
1122
1123
1124 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1119.659 kb on + strand, within Pf6N2E2_949 at 1119.659 kb on + strand, within Pf6N2E2_949 at 1119.660 kb on - strand, within Pf6N2E2_949 at 1119.741 kb on + strand, within Pf6N2E2_949 at 1119.742 kb on - strand, within Pf6N2E2_949 at 1119.742 kb on - strand, within Pf6N2E2_949 at 1119.742 kb on - strand, within Pf6N2E2_949 at 1119.742 kb on - strand, within Pf6N2E2_949 at 1119.879 kb on + strand, within Pf6N2E2_949 at 1119.880 kb on - strand, within Pf6N2E2_949 at 1119.900 kb on + strand, within Pf6N2E2_949 at 1119.901 kb on - strand, within Pf6N2E2_949 at 1119.996 kb on + strand at 1119.997 kb on - strand at 1119.997 kb on - strand at 1119.998 kb on + strand at 1119.999 kb on - strand at 1119.999 kb on - strand at 1120.091 kb on + strand at 1120.112 kb on + strand at 1120.210 kb on - strand at 1120.215 kb on + strand at 1120.216 kb on - strand at 1120.303 kb on + strand at 1120.352 kb on + strand at 1120.353 kb on - strand at 1120.449 kb on + strand at 1120.449 kb on + strand at 1120.450 kb on - strand at 1120.550 kb on + strand at 1120.573 kb on + strand at 1120.573 kb on + strand at 1120.708 kb on + strand at 1120.774 kb on + strand at 1120.774 kb on + strand at 1120.774 kb on + strand at 1121.123 kb on - strand, within Pf6N2E2_950 at 1121.161 kb on + strand, within Pf6N2E2_950 at 1121.161 kb on + strand, within Pf6N2E2_950 at 1121.161 kb on + strand, within Pf6N2E2_950 at 1121.161 kb on + strand, within Pf6N2E2_950 at 1121.161 kb on + strand, within Pf6N2E2_950 at 1121.162 kb on - strand, within Pf6N2E2_950 at 1121.162 kb on - strand, within Pf6N2E2_950 at 1121.162 kb on - strand, within Pf6N2E2_950 at 1121.652 kb on - strand, within Pf6N2E2_950 at 1121.681 kb on - strand, within Pf6N2E2_950 at 1121.724 kb on + strand, within Pf6N2E2_950 at 1121.724 kb on + strand, within Pf6N2E2_950 at 1121.724 kb on + strand, within Pf6N2E2_950 at 1121.724 kb on + strand, within Pf6N2E2_950 at 1121.724 kb on + strand, within Pf6N2E2_950 at 1121.724 kb on + strand, within Pf6N2E2_950 at 1121.725 kb on - strand, within Pf6N2E2_950 at 1121.725 kb on - strand, within Pf6N2E2_950 at 1121.725 kb on - strand, within Pf6N2E2_950 at 1121.725 kb on - strand, within Pf6N2E2_950 at 1122.001 kb on + strand, within Pf6N2E2_950 at 1122.002 kb on - strand, within Pf6N2E2_950 at 1122.145 kb on + strand, within Pf6N2E2_950 at 1122.145 kb on + strand, within Pf6N2E2_950 at 1122.167 kb on - strand, within Pf6N2E2_950 at 1122.285 kb on + strand, within Pf6N2E2_950 at 1122.285 kb on + strand, within Pf6N2E2_950 at 1122.286 kb on - strand, within Pf6N2E2_950 at 1122.286 kb on - strand, within Pf6N2E2_950 at 1122.326 kb on + strand, within Pf6N2E2_950 at 1122.327 kb on - strand, within Pf6N2E2_950 at 1122.580 kb on + strand, within Pf6N2E2_950 at 1122.581 kb on - strand, within Pf6N2E2_950 at 1122.646 kb on + strand, within Pf6N2E2_950 at 1122.646 kb on + strand, within Pf6N2E2_950 at 1122.782 kb on - strand, within Pf6N2E2_950 at 1122.901 kb on + strand, within Pf6N2E2_950 at 1122.901 kb on + strand, within Pf6N2E2_950 at 1122.901 kb on + strand, within Pf6N2E2_950 at 1122.902 kb on - strand, within Pf6N2E2_950 at 1122.902 kb on - strand, within Pf6N2E2_950 at 1122.902 kb on - strand, within Pf6N2E2_950 at 1122.902 kb on - strand, within Pf6N2E2_950 at 1122.902 kb on - strand, within Pf6N2E2_950 at 1122.943 kb on + strand, within Pf6N2E2_950 at 1122.943 kb on + strand, within Pf6N2E2_950 at 1122.943 kb on + strand, within Pf6N2E2_950 at 1122.943 kb on + strand, within Pf6N2E2_950 at 1122.944 kb on - strand, within Pf6N2E2_950 at 1122.964 kb on + strand, within Pf6N2E2_950 at 1123.181 kb on - strand at 1123.225 kb on + strand at 1123.225 kb on + strand at 1123.226 kb on - strand at 1123.226 kb on - strand at 1123.226 kb on - strand at 1123.226 kb on - strand at 1123.226 kb on - strand at 1123.323 kb on + strand at 1123.323 kb on + strand at 1123.323 kb on + strand at 1123.324 kb on - strand at 1123.357 kb on - strand, within Pf6N2E2_951 at 1123.371 kb on + strand, within Pf6N2E2_951 at 1123.372 kb on - strand, within Pf6N2E2_951 at 1123.434 kb on + strand, within Pf6N2E2_951 at 1123.436 kb on + strand, within Pf6N2E2_951 at 1123.436 kb on + strand, within Pf6N2E2_951 at 1123.436 kb on + strand, within Pf6N2E2_951 at 1123.436 kb on + strand, within Pf6N2E2_951 at 1123.436 kb on + strand, within Pf6N2E2_951 at 1123.436 kb on + strand, within Pf6N2E2_951 at 1123.436 kb on + strand, within Pf6N2E2_951 at 1123.436 kb on + strand, within Pf6N2E2_951 at 1123.436 kb on + strand, within Pf6N2E2_951 at 1123.436 kb on + strand, within Pf6N2E2_951 at 1123.436 kb on + strand, within Pf6N2E2_951 at 1123.437 kb on - strand, within Pf6N2E2_951 at 1123.437 kb on - strand, within Pf6N2E2_951 at 1123.437 kb on - strand, within Pf6N2E2_951 at 1123.440 kb on + strand, within Pf6N2E2_951 at 1123.440 kb on + strand, within Pf6N2E2_951 at 1123.440 kb on + strand, within Pf6N2E2_951 at 1123.440 kb on + strand, within Pf6N2E2_951 at 1123.440 kb on + strand, within Pf6N2E2_951 at 1123.441 kb on - strand, within Pf6N2E2_951 at 1123.441 kb on - strand, within Pf6N2E2_951 at 1123.441 kb on - strand, within Pf6N2E2_951 at 1123.538 kb on + strand, within Pf6N2E2_951 at 1123.538 kb on + strand, within Pf6N2E2_951 at 1123.539 kb on - strand, within Pf6N2E2_951 at 1123.559 kb on + strand, within Pf6N2E2_951 at 1123.559 kb on + strand, within Pf6N2E2_951 at 1123.822 kb on + strand at 1123.822 kb on + strand at 1123.823 kb on - strand at 1123.823 kb on - strand at 1123.846 kb on + strand at 1123.847 kb on - strand at 1124.041 kb on + strand, within Pf6N2E2_952 at 1124.041 kb on + strand, within Pf6N2E2_952 at 1124.192 kb on + strand, within Pf6N2E2_952 at 1124.192 kb on + strand, within Pf6N2E2_952
Per-strain Table
Position Strand Gene LocusTag Fraction Fluorophosphate 0.125 M remove 1,119,659 + Pf6N2E2_949 0.72 +0.1 1,119,659 + Pf6N2E2_949 0.72 -0.8 1,119,660 - Pf6N2E2_949 0.72 -0.2 1,119,741 + Pf6N2E2_949 0.78 +0.3 1,119,742 - Pf6N2E2_949 0.78 +0.1 1,119,742 - Pf6N2E2_949 0.78 -0.0 1,119,742 - Pf6N2E2_949 0.78 +0.7 1,119,742 - Pf6N2E2_949 0.78 +0.6 1,119,879 + Pf6N2E2_949 0.88 +0.0 1,119,880 - Pf6N2E2_949 0.88 -0.3 1,119,900 + Pf6N2E2_949 0.89 -1.6 1,119,901 - Pf6N2E2_949 0.89 +0.6 1,119,996 + +0.6 1,119,997 - +0.8 1,119,997 - -0.2 1,119,998 + +0.8 1,119,999 - -0.1 1,119,999 - -0.2 1,120,091 + +0.2 1,120,112 + -0.4 1,120,210 - -0.6 1,120,215 + +1.8 1,120,216 - -0.4 1,120,303 + +1.4 1,120,352 + +0.7 1,120,353 - +0.3 1,120,449 + -1.4 1,120,449 + +2.3 1,120,450 - +0.1 1,120,550 + -2.7 1,120,573 + -0.2 1,120,573 + -0.2 1,120,708 + -1.9 1,120,774 + +0.9 1,120,774 + +0.7 1,120,774 + +0.2 1,121,123 - Pf6N2E2_950 0.20 +0.7 1,121,161 + Pf6N2E2_950 0.21 +0.8 1,121,161 + Pf6N2E2_950 0.21 -0.2 1,121,161 + Pf6N2E2_950 0.21 +0.6 1,121,161 + Pf6N2E2_950 0.21 +0.5 1,121,161 + Pf6N2E2_950 0.21 +1.0 1,121,162 - Pf6N2E2_950 0.21 +1.7 1,121,162 - Pf6N2E2_950 0.21 +0.0 1,121,162 - Pf6N2E2_950 0.21 +1.8 1,121,652 - Pf6N2E2_950 0.39 +1.4 1,121,681 - Pf6N2E2_950 0.40 +2.7 1,121,724 + Pf6N2E2_950 0.42 +0.1 1,121,724 + Pf6N2E2_950 0.42 -1.6 1,121,724 + Pf6N2E2_950 0.42 +0.2 1,121,724 + Pf6N2E2_950 0.42 +0.3 1,121,724 + Pf6N2E2_950 0.42 -1.9 1,121,724 + Pf6N2E2_950 0.42 +1.2 1,121,725 - Pf6N2E2_950 0.42 +1.8 1,121,725 - Pf6N2E2_950 0.42 +0.5 1,121,725 - Pf6N2E2_950 0.42 -1.4 1,121,725 - Pf6N2E2_950 0.42 +1.4 1,122,001 + Pf6N2E2_950 0.52 +0.3 1,122,002 - Pf6N2E2_950 0.52 +0.6 1,122,145 + Pf6N2E2_950 0.57 +1.3 1,122,145 + Pf6N2E2_950 0.57 +1.4 1,122,167 - Pf6N2E2_950 0.58 +0.4 1,122,285 + Pf6N2E2_950 0.62 +0.6 1,122,285 + Pf6N2E2_950 0.62 -0.6 1,122,286 - Pf6N2E2_950 0.62 -1.0 1,122,286 - Pf6N2E2_950 0.62 +0.6 1,122,326 + Pf6N2E2_950 0.64 +1.4 1,122,327 - Pf6N2E2_950 0.64 -0.5 1,122,580 + Pf6N2E2_950 0.73 +0.1 1,122,581 - Pf6N2E2_950 0.73 -0.3 1,122,646 + Pf6N2E2_950 0.75 +0.4 1,122,646 + Pf6N2E2_950 0.75 +0.2 1,122,782 - Pf6N2E2_950 0.80 +0.8 1,122,901 + Pf6N2E2_950 0.85 +1.4 1,122,901 + Pf6N2E2_950 0.85 +0.5 1,122,901 + Pf6N2E2_950 0.85 -1.5 1,122,902 - Pf6N2E2_950 0.85 +1.8 1,122,902 - Pf6N2E2_950 0.85 -0.1 1,122,902 - Pf6N2E2_950 0.85 -0.2 1,122,902 - Pf6N2E2_950 0.85 +0.7 1,122,902 - Pf6N2E2_950 0.85 -0.3 1,122,943 + Pf6N2E2_950 0.86 +0.3 1,122,943 + Pf6N2E2_950 0.86 -0.3 1,122,943 + Pf6N2E2_950 0.86 -0.7 1,122,943 + Pf6N2E2_950 0.86 +0.9 1,122,944 - Pf6N2E2_950 0.86 -0.6 1,122,964 + Pf6N2E2_950 0.87 +1.0 1,123,181 - +0.2 1,123,225 + +0.5 1,123,225 + -1.2 1,123,226 - +0.1 1,123,226 - -1.0 1,123,226 - +0.1 1,123,226 - +0.4 1,123,226 - +2.1 1,123,323 + +0.2 1,123,323 + +0.8 1,123,323 + -2.0 1,123,324 - +0.7 1,123,357 - Pf6N2E2_951 0.11 +1.2 1,123,371 + Pf6N2E2_951 0.16 -0.8 1,123,372 - Pf6N2E2_951 0.16 -0.3 1,123,434 + Pf6N2E2_951 0.36 -1.1 1,123,436 + Pf6N2E2_951 0.37 +0.7 1,123,436 + Pf6N2E2_951 0.37 +0.2 1,123,436 + Pf6N2E2_951 0.37 +0.8 1,123,436 + Pf6N2E2_951 0.37 +1.1 1,123,436 + Pf6N2E2_951 0.37 -0.9 1,123,436 + Pf6N2E2_951 0.37 +0.6 1,123,436 + Pf6N2E2_951 0.37 +1.4 1,123,436 + Pf6N2E2_951 0.37 -1.4 1,123,436 + Pf6N2E2_951 0.37 -0.1 1,123,436 + Pf6N2E2_951 0.37 +1.1 1,123,436 + Pf6N2E2_951 0.37 -1.4 1,123,437 - Pf6N2E2_951 0.37 -0.4 1,123,437 - Pf6N2E2_951 0.37 +2.6 1,123,437 - Pf6N2E2_951 0.37 +0.3 1,123,440 + Pf6N2E2_951 0.38 +0.5 1,123,440 + Pf6N2E2_951 0.38 +0.3 1,123,440 + Pf6N2E2_951 0.38 +1.4 1,123,440 + Pf6N2E2_951 0.38 -0.6 1,123,440 + Pf6N2E2_951 0.38 -1.9 1,123,441 - Pf6N2E2_951 0.39 +1.3 1,123,441 - Pf6N2E2_951 0.39 -1.3 1,123,441 - Pf6N2E2_951 0.39 -0.0 1,123,538 + Pf6N2E2_951 0.70 +1.2 1,123,538 + Pf6N2E2_951 0.70 -0.7 1,123,539 - Pf6N2E2_951 0.70 +1.8 1,123,559 + Pf6N2E2_951 0.77 +3.0 1,123,559 + Pf6N2E2_951 0.77 -0.1 1,123,822 + +0.2 1,123,822 + +1.0 1,123,823 - +1.4 1,123,823 - +1.1 1,123,846 + -0.2 1,123,847 - +0.4 1,124,041 + Pf6N2E2_952 0.22 -0.7 1,124,041 + Pf6N2E2_952 0.22 +0.4 1,124,192 + Pf6N2E2_952 0.32 -0.0 1,124,192 + Pf6N2E2_952 0.32 -0.5
Or see this region's nucleotide sequence