Experiment: Fluorophosphate 0.125 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1587 and Pf6N2E2_1588 are separated by 81 nucleotides Pf6N2E2_1588 and Pf6N2E2_1589 overlap by 11 nucleotides Pf6N2E2_1589 and Pf6N2E2_1590 are separated by 3 nucleotides
Pf6N2E2_1587 - Dihydroflavonol-4-reductase (EC 1.1.1.219), at 1,856,807 to 1,857,820
_1587
Pf6N2E2_1588 - Long-chain-fatty-acid--CoA ligase (EC 6.2.1.3), at 1,857,902 to 1,859,422
_1588
Pf6N2E2_1589 - Phosphoserine phosphatase (EC 3.1.3.3), at 1,859,412 to 1,860,023
_1589
Pf6N2E2_1590 - pentachlorophenol 4-monooxygenase, at 1,860,027 to 1,861,790
_1590
Position (kb)
1857
1858
1859
1860 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1857.047 kb on + strand, within Pf6N2E2_1587 at 1857.078 kb on - strand, within Pf6N2E2_1587 at 1857.252 kb on + strand, within Pf6N2E2_1587 at 1857.356 kb on - strand, within Pf6N2E2_1587 at 1857.408 kb on + strand, within Pf6N2E2_1587 at 1857.616 kb on + strand, within Pf6N2E2_1587 at 1857.617 kb on - strand, within Pf6N2E2_1587 at 1857.617 kb on - strand, within Pf6N2E2_1587 at 1857.621 kb on - strand, within Pf6N2E2_1587 at 1857.621 kb on - strand, within Pf6N2E2_1587 at 1857.621 kb on - strand, within Pf6N2E2_1587 at 1857.630 kb on - strand, within Pf6N2E2_1587 at 1857.800 kb on - strand at 1857.834 kb on + strand at 1857.834 kb on + strand at 1857.919 kb on + strand at 1857.920 kb on - strand at 1858.013 kb on - strand at 1858.060 kb on + strand, within Pf6N2E2_1588 at 1858.092 kb on + strand, within Pf6N2E2_1588 at 1858.092 kb on + strand, within Pf6N2E2_1588 at 1858.133 kb on + strand, within Pf6N2E2_1588 at 1858.133 kb on + strand, within Pf6N2E2_1588 at 1858.178 kb on + strand, within Pf6N2E2_1588 at 1858.179 kb on - strand at 1858.179 kb on - strand, within Pf6N2E2_1588 at 1858.479 kb on - strand, within Pf6N2E2_1588 at 1858.730 kb on - strand, within Pf6N2E2_1588 at 1858.788 kb on - strand, within Pf6N2E2_1588 at 1858.842 kb on + strand, within Pf6N2E2_1588 at 1858.916 kb on - strand, within Pf6N2E2_1588 at 1859.023 kb on - strand, within Pf6N2E2_1588 at 1859.023 kb on - strand, within Pf6N2E2_1588 at 1859.049 kb on - strand, within Pf6N2E2_1588 at 1859.252 kb on + strand, within Pf6N2E2_1588 at 1859.253 kb on - strand, within Pf6N2E2_1588 at 1859.351 kb on + strand at 1859.389 kb on + strand at 1859.390 kb on - strand at 1859.504 kb on + strand, within Pf6N2E2_1589 at 1859.619 kb on + strand, within Pf6N2E2_1589 at 1859.664 kb on + strand, within Pf6N2E2_1589 at 1859.665 kb on - strand, within Pf6N2E2_1589 at 1859.665 kb on - strand, within Pf6N2E2_1589 at 1859.665 kb on - strand, within Pf6N2E2_1589 at 1859.779 kb on - strand, within Pf6N2E2_1589 at 1859.779 kb on - strand, within Pf6N2E2_1589 at 1859.814 kb on + strand, within Pf6N2E2_1589 at 1859.815 kb on - strand, within Pf6N2E2_1589 at 1859.885 kb on - strand, within Pf6N2E2_1589 at 1859.946 kb on + strand, within Pf6N2E2_1589 at 1859.946 kb on + strand, within Pf6N2E2_1589 at 1859.946 kb on + strand, within Pf6N2E2_1589 at 1859.947 kb on - strand, within Pf6N2E2_1589 at 1859.947 kb on - strand, within Pf6N2E2_1589 at 1859.947 kb on - strand, within Pf6N2E2_1589 at 1860.006 kb on + strand at 1860.006 kb on + strand at 1860.007 kb on - strand at 1860.019 kb on - strand at 1860.327 kb on + strand, within Pf6N2E2_1590 at 1860.340 kb on - strand, within Pf6N2E2_1590
Per-strain Table
Position Strand Gene LocusTag Fraction Fluorophosphate 0.125 M remove 1,857,047 + Pf6N2E2_1587 0.24 +1.4 1,857,078 - Pf6N2E2_1587 0.27 +0.3 1,857,252 + Pf6N2E2_1587 0.44 -0.5 1,857,356 - Pf6N2E2_1587 0.54 -1.6 1,857,408 + Pf6N2E2_1587 0.59 -1.8 1,857,616 + Pf6N2E2_1587 0.80 +0.4 1,857,617 - Pf6N2E2_1587 0.80 -0.3 1,857,617 - Pf6N2E2_1587 0.80 -1.1 1,857,621 - Pf6N2E2_1587 0.80 +1.4 1,857,621 - Pf6N2E2_1587 0.80 -0.2 1,857,621 - Pf6N2E2_1587 0.80 -2.3 1,857,630 - Pf6N2E2_1587 0.81 -0.8 1,857,800 - +0.9 1,857,834 + -1.6 1,857,834 + +0.6 1,857,919 + -0.7 1,857,920 - +1.2 1,858,013 - +1.1 1,858,060 + Pf6N2E2_1588 0.10 +1.5 1,858,092 + Pf6N2E2_1588 0.12 +0.1 1,858,092 + Pf6N2E2_1588 0.12 -0.3 1,858,133 + Pf6N2E2_1588 0.15 -1.5 1,858,133 + Pf6N2E2_1588 0.15 -1.3 1,858,178 + Pf6N2E2_1588 0.18 -2.9 1,858,179 - +1.1 1,858,179 - Pf6N2E2_1588 0.18 -1.9 1,858,479 - Pf6N2E2_1588 0.38 -0.9 1,858,730 - Pf6N2E2_1588 0.54 -0.5 1,858,788 - Pf6N2E2_1588 0.58 +2.4 1,858,842 + Pf6N2E2_1588 0.62 -0.4 1,858,916 - Pf6N2E2_1588 0.67 -1.2 1,859,023 - Pf6N2E2_1588 0.74 +2.8 1,859,023 - Pf6N2E2_1588 0.74 -0.7 1,859,049 - Pf6N2E2_1588 0.75 -2.3 1,859,252 + Pf6N2E2_1588 0.89 -0.8 1,859,253 - Pf6N2E2_1588 0.89 +0.6 1,859,351 + +0.3 1,859,389 + +0.8 1,859,390 - +1.2 1,859,504 + Pf6N2E2_1589 0.15 -1.4 1,859,619 + Pf6N2E2_1589 0.34 -0.3 1,859,664 + Pf6N2E2_1589 0.41 +0.3 1,859,665 - Pf6N2E2_1589 0.41 +1.8 1,859,665 - Pf6N2E2_1589 0.41 +0.4 1,859,665 - Pf6N2E2_1589 0.41 -0.4 1,859,779 - Pf6N2E2_1589 0.60 +0.4 1,859,779 - Pf6N2E2_1589 0.60 -0.4 1,859,814 + Pf6N2E2_1589 0.66 +0.2 1,859,815 - Pf6N2E2_1589 0.66 +0.3 1,859,885 - Pf6N2E2_1589 0.77 -1.0 1,859,946 + Pf6N2E2_1589 0.87 -1.3 1,859,946 + Pf6N2E2_1589 0.87 +0.5 1,859,946 + Pf6N2E2_1589 0.87 +1.8 1,859,947 - Pf6N2E2_1589 0.87 +1.7 1,859,947 - Pf6N2E2_1589 0.87 +2.0 1,859,947 - Pf6N2E2_1589 0.87 -0.2 1,860,006 + -0.5 1,860,006 + +0.7 1,860,007 - +1.4 1,860,019 - +1.2 1,860,327 + Pf6N2E2_1590 0.17 +0.4 1,860,340 - Pf6N2E2_1590 0.18 -1.0
Or see this region's nucleotide sequence