Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_108

Experiment: Fluorophosphate 0.125 M

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_106 and Pf6N2E2_107 are separated by 10 nucleotidesPf6N2E2_107 and Pf6N2E2_108 are separated by 48 nucleotidesPf6N2E2_108 and Pf6N2E2_109 are separated by 119 nucleotides Pf6N2E2_106 - Glycerol-3-phosphate ABC transporter, permease protein UgpE (TC 3.A.1.1.3), at 151,621 to 152,421 _106 Pf6N2E2_107 - Probable transmembrane protein, at 152,432 to 152,704 _107 Pf6N2E2_108 - Glycerol-3-phosphate ABC transporter, permease protein UgpA (TC 3.A.1.1.3), at 152,753 to 154,495 _108 Pf6N2E2_109 - Hydrolases of the alpha/beta superfamily, at 154,615 to 155,490 _109 Position (kb) 152 153 154 155Strain fitness (log2 ratio) -2 -1 0 1 2at 151.768 kb on + strand, within Pf6N2E2_106at 151.768 kb on + strand, within Pf6N2E2_106at 151.768 kb on + strand, within Pf6N2E2_106at 151.769 kb on - strand, within Pf6N2E2_106at 151.798 kb on + strand, within Pf6N2E2_106at 151.823 kb on - strand, within Pf6N2E2_106at 151.825 kb on + strand, within Pf6N2E2_106at 151.826 kb on - strand, within Pf6N2E2_106at 151.826 kb on - strand, within Pf6N2E2_106at 151.984 kb on + strand, within Pf6N2E2_106at 151.984 kb on + strand, within Pf6N2E2_106at 151.985 kb on - strand, within Pf6N2E2_106at 151.985 kb on - strand, within Pf6N2E2_106at 152.117 kb on - strand, within Pf6N2E2_106at 152.703 kb on - strandat 152.731 kb on + strandat 152.752 kb on + strandat 152.753 kb on - strandat 152.753 kb on - strandat 152.753 kb on - strandat 152.753 kb on - strandat 152.782 kb on + strandat 152.783 kb on - strandat 152.783 kb on - strandat 152.893 kb on + strandat 152.893 kb on + strandat 153.127 kb on + strand, within Pf6N2E2_108at 153.127 kb on + strand, within Pf6N2E2_108at 153.127 kb on + strand, within Pf6N2E2_108at 153.127 kb on + strand, within Pf6N2E2_108at 153.127 kb on + strand, within Pf6N2E2_108at 153.127 kb on + strand, within Pf6N2E2_108at 153.127 kb on + strand, within Pf6N2E2_108at 153.128 kb on - strand, within Pf6N2E2_108at 153.128 kb on - strand, within Pf6N2E2_108at 153.128 kb on - strand, within Pf6N2E2_108at 153.128 kb on - strand, within Pf6N2E2_108at 153.128 kb on - strand, within Pf6N2E2_108at 153.128 kb on - strand, within Pf6N2E2_108at 153.128 kb on - strand, within Pf6N2E2_108at 153.131 kb on + strand, within Pf6N2E2_108at 153.131 kb on + strand, within Pf6N2E2_108at 153.131 kb on + strand, within Pf6N2E2_108at 153.131 kb on + strand, within Pf6N2E2_108at 153.132 kb on - strand, within Pf6N2E2_108at 153.132 kb on - strand, within Pf6N2E2_108at 153.132 kb on - strand, within Pf6N2E2_108at 153.132 kb on - strand, within Pf6N2E2_108at 153.132 kb on - strand, within Pf6N2E2_108at 153.132 kb on - strand, within Pf6N2E2_108at 153.132 kb on - strand, within Pf6N2E2_108at 153.132 kb on - strand, within Pf6N2E2_108at 153.132 kb on - strand, within Pf6N2E2_108at 153.132 kb on - strand, within Pf6N2E2_108at 153.268 kb on + strand, within Pf6N2E2_108at 153.278 kb on - strand, within Pf6N2E2_108at 153.336 kb on - strand, within Pf6N2E2_108at 153.395 kb on + strand, within Pf6N2E2_108at 153.395 kb on + strand, within Pf6N2E2_108at 153.396 kb on - strand, within Pf6N2E2_108at 153.396 kb on - strand, within Pf6N2E2_108at 153.396 kb on - strand, within Pf6N2E2_108at 153.435 kb on - strand, within Pf6N2E2_108at 153.435 kb on - strand, within Pf6N2E2_108at 153.435 kb on - strand, within Pf6N2E2_108at 153.435 kb on - strand, within Pf6N2E2_108at 153.435 kb on - strand, within Pf6N2E2_108at 153.495 kb on - strand, within Pf6N2E2_108at 153.710 kb on + strand, within Pf6N2E2_108at 153.711 kb on - strand, within Pf6N2E2_108at 153.711 kb on - strand, within Pf6N2E2_108at 153.711 kb on - strand, within Pf6N2E2_108at 153.711 kb on - strand, within Pf6N2E2_108at 153.731 kb on + strand, within Pf6N2E2_108at 153.731 kb on + strand, within Pf6N2E2_108at 153.731 kb on + strand, within Pf6N2E2_108at 153.732 kb on - strand, within Pf6N2E2_108at 153.732 kb on - strand, within Pf6N2E2_108at 153.732 kb on - strand, within Pf6N2E2_108at 153.818 kb on + strand, within Pf6N2E2_108at 153.818 kb on + strand, within Pf6N2E2_108at 153.917 kb on + strand, within Pf6N2E2_108at 153.918 kb on - strand, within Pf6N2E2_108at 153.945 kb on - strand, within Pf6N2E2_108at 154.014 kb on - strand, within Pf6N2E2_108at 154.116 kb on + strand, within Pf6N2E2_108at 154.383 kb on - strandat 154.436 kb on - strandat 154.493 kb on + strandat 154.493 kb on + strandat 154.493 kb on + strandat 154.493 kb on + strandat 154.493 kb on + strandat 154.494 kb on - strandat 154.494 kb on - strandat 154.731 kb on + strand, within Pf6N2E2_109at 154.732 kb on - strand, within Pf6N2E2_109at 154.920 kb on + strand, within Pf6N2E2_109at 154.920 kb on + strand, within Pf6N2E2_109at 154.920 kb on + strand, within Pf6N2E2_109at 154.921 kb on - strand, within Pf6N2E2_109at 154.921 kb on - strand, within Pf6N2E2_109at 154.921 kb on - strand, within Pf6N2E2_109at 154.921 kb on - strand, within Pf6N2E2_109at 154.921 kb on - strand, within Pf6N2E2_109at 154.921 kb on - strand, within Pf6N2E2_109at 155.069 kb on + strand, within Pf6N2E2_109at 155.070 kb on - strand, within Pf6N2E2_109at 155.070 kb on - strand, within Pf6N2E2_109at 155.070 kb on - strand, within Pf6N2E2_109at 155.102 kb on - strand, within Pf6N2E2_109at 155.138 kb on + strand, within Pf6N2E2_109at 155.138 kb on + strand, within Pf6N2E2_109at 155.138 kb on + strand, within Pf6N2E2_109at 155.235 kb on + strand, within Pf6N2E2_109at 155.279 kb on + strand, within Pf6N2E2_109at 155.280 kb on - strand, within Pf6N2E2_109at 155.448 kb on + strandat 155.448 kb on + strandat 155.448 kb on + strandat 155.448 kb on + strandat 155.448 kb on + strandat 155.448 kb on + strandat 155.449 kb on - strandat 155.449 kb on - strandat 155.449 kb on - strandat 155.449 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Fluorophosphate 0.125 M
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151,768 + Pf6N2E2_106 0.18 -0.4
151,768 + Pf6N2E2_106 0.18 +0.3
151,768 + Pf6N2E2_106 0.18 +0.4
151,769 - Pf6N2E2_106 0.18 +0.1
151,798 + Pf6N2E2_106 0.22 -2.2
151,823 - Pf6N2E2_106 0.25 -2.5
151,825 + Pf6N2E2_106 0.25 +0.8
151,826 - Pf6N2E2_106 0.26 -0.0
151,826 - Pf6N2E2_106 0.26 +0.9
151,984 + Pf6N2E2_106 0.45 +0.5
151,984 + Pf6N2E2_106 0.45 +0.5
151,985 - Pf6N2E2_106 0.45 -0.6
151,985 - Pf6N2E2_106 0.45 +1.5
152,117 - Pf6N2E2_106 0.62 +0.6
152,703 - +0.9
152,731 + +0.5
152,752 + +0.3
152,753 - +0.7
152,753 - +0.7
152,753 - -1.4
152,753 - +1.8
152,782 + +0.5
152,783 - +1.4
152,783 - -1.8
152,893 + -1.4
152,893 + +0.3
153,127 + Pf6N2E2_108 0.21 +0.3
153,127 + Pf6N2E2_108 0.21 +0.5
153,127 + Pf6N2E2_108 0.21 -0.5
153,127 + Pf6N2E2_108 0.21 -0.5
153,127 + Pf6N2E2_108 0.21 +0.7
153,127 + Pf6N2E2_108 0.21 -0.3
153,127 + Pf6N2E2_108 0.21 +0.7
153,128 - Pf6N2E2_108 0.22 +1.9
153,128 - Pf6N2E2_108 0.22 -0.5
153,128 - Pf6N2E2_108 0.22 -0.6
153,128 - Pf6N2E2_108 0.22 -1.3
153,128 - Pf6N2E2_108 0.22 -0.0
153,128 - Pf6N2E2_108 0.22 -0.6
153,128 - Pf6N2E2_108 0.22 -0.8
153,131 + Pf6N2E2_108 0.22 -0.3
153,131 + Pf6N2E2_108 0.22 -0.4
153,131 + Pf6N2E2_108 0.22 -0.2
153,131 + Pf6N2E2_108 0.22 -0.3
153,132 - Pf6N2E2_108 0.22 -0.2
153,132 - Pf6N2E2_108 0.22 -0.3
153,132 - Pf6N2E2_108 0.22 +0.2
153,132 - Pf6N2E2_108 0.22 -1.4
153,132 - Pf6N2E2_108 0.22 -0.3
153,132 - Pf6N2E2_108 0.22 -1.3
153,132 - Pf6N2E2_108 0.22 +0.2
153,132 - Pf6N2E2_108 0.22 +0.1
153,132 - Pf6N2E2_108 0.22 +1.0
153,132 - Pf6N2E2_108 0.22 -0.2
153,268 + Pf6N2E2_108 0.30 -0.0
153,278 - Pf6N2E2_108 0.30 +0.7
153,336 - Pf6N2E2_108 0.33 +0.1
153,395 + Pf6N2E2_108 0.37 +0.2
153,395 + Pf6N2E2_108 0.37 -1.2
153,396 - Pf6N2E2_108 0.37 -0.9
153,396 - Pf6N2E2_108 0.37 -0.9
153,396 - Pf6N2E2_108 0.37 -1.0
153,435 - Pf6N2E2_108 0.39 +0.8
153,435 - Pf6N2E2_108 0.39 -0.7
153,435 - Pf6N2E2_108 0.39 +0.4
153,435 - Pf6N2E2_108 0.39 -0.3
153,435 - Pf6N2E2_108 0.39 -0.1
153,495 - Pf6N2E2_108 0.43 -0.3
153,710 + Pf6N2E2_108 0.55 +0.5
153,711 - Pf6N2E2_108 0.55 -0.3
153,711 - Pf6N2E2_108 0.55 +0.1
153,711 - Pf6N2E2_108 0.55 +0.5
153,711 - Pf6N2E2_108 0.55 +0.0
153,731 + Pf6N2E2_108 0.56 +0.2
153,731 + Pf6N2E2_108 0.56 -0.4
153,731 + Pf6N2E2_108 0.56 -1.4
153,732 - Pf6N2E2_108 0.56 -0.3
153,732 - Pf6N2E2_108 0.56 +1.1
153,732 - Pf6N2E2_108 0.56 -2.4
153,818 + Pf6N2E2_108 0.61 +1.1
153,818 + Pf6N2E2_108 0.61 +1.5
153,917 + Pf6N2E2_108 0.67 -1.7
153,918 - Pf6N2E2_108 0.67 -2.0
153,945 - Pf6N2E2_108 0.68 -0.7
154,014 - Pf6N2E2_108 0.72 +0.1
154,116 + Pf6N2E2_108 0.78 +0.1
154,383 - -1.3
154,436 - +0.0
154,493 + +0.1
154,493 + -0.9
154,493 + +0.0
154,493 + +0.3
154,493 + -0.7
154,494 - +0.7
154,494 - -1.4
154,731 + Pf6N2E2_109 0.13 +0.3
154,732 - Pf6N2E2_109 0.13 -0.7
154,920 + Pf6N2E2_109 0.35 +0.3
154,920 + Pf6N2E2_109 0.35 -0.4
154,920 + Pf6N2E2_109 0.35 -1.5
154,921 - Pf6N2E2_109 0.35 -0.3
154,921 - Pf6N2E2_109 0.35 -0.8
154,921 - Pf6N2E2_109 0.35 +0.2
154,921 - Pf6N2E2_109 0.35 +0.8
154,921 - Pf6N2E2_109 0.35 +0.2
154,921 - Pf6N2E2_109 0.35 +0.1
155,069 + Pf6N2E2_109 0.52 -0.3
155,070 - Pf6N2E2_109 0.52 -0.7
155,070 - Pf6N2E2_109 0.52 -0.0
155,070 - Pf6N2E2_109 0.52 -0.7
155,102 - Pf6N2E2_109 0.56 +1.0
155,138 + Pf6N2E2_109 0.60 -0.1
155,138 + Pf6N2E2_109 0.60 -1.6
155,138 + Pf6N2E2_109 0.60 +0.2
155,235 + Pf6N2E2_109 0.71 +0.1
155,279 + Pf6N2E2_109 0.76 -0.8
155,280 - Pf6N2E2_109 0.76 +0.2
155,448 + +0.5
155,448 + +1.3
155,448 + -1.8
155,448 + +0.0
155,448 + -0.3
155,448 + -1.4
155,449 - -1.4
155,449 - -0.4
155,449 - -1.4
155,449 - -1.1

Or see this region's nucleotide sequence