Experiment: Formate McCas no stress control 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS03635 and MMP_RS03640 are separated by 134 nucleotides MMP_RS03640 and MMP_RS03645 are separated by 95 nucleotides
MMP_RS03635: MMP_RS03635 - beta-CASP ribonuclease aCPSF1, at 682,145 to 684,052
_RS03635
MMP_RS03640: MMP_RS03640 - archaeal proteasome endopeptidase complex subunit beta, at 684,187 to 684,846
_RS03640
MMP_RS03645: MMP_RS03645 - proline--tRNA ligase, at 684,942 to 686,324
_RS03645
Position (kb)
684
685 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 684.066 kb on + strand at 684.072 kb on + strand at 684.147 kb on - strand at 684.148 kb on + strand at 684.172 kb on - strand at 684.272 kb on + strand, within MMP_RS03640 at 684.293 kb on - strand, within MMP_RS03640 at 684.293 kb on - strand, within MMP_RS03640 at 684.552 kb on - strand, within MMP_RS03640 at 684.631 kb on - strand, within MMP_RS03640 at 684.631 kb on - strand, within MMP_RS03640 at 684.682 kb on - strand, within MMP_RS03640 at 684.686 kb on - strand, within MMP_RS03640 at 684.860 kb on + strand at 684.860 kb on + strand at 684.862 kb on - strand at 684.875 kb on + strand at 684.893 kb on + strand at 684.943 kb on - strand at 684.944 kb on + strand at 684.944 kb on + strand at 684.966 kb on + strand at 684.983 kb on + strand at 685.193 kb on + strand at 685.699 kb on - strand at 685.701 kb on - strand at 685.772 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Formate McCas no stress control 2 remove 684,066 + -2.3 684,072 + -2.0 684,147 - -1.6 684,148 + -1.1 684,172 - -0.6 684,272 + MMP_RS03640 0.13 -2.2 684,293 - MMP_RS03640 0.16 -0.4 684,293 - MMP_RS03640 0.16 -0.6 684,552 - MMP_RS03640 0.55 -0.3 684,631 - MMP_RS03640 0.67 -1.8 684,631 - MMP_RS03640 0.67 -1.3 684,682 - MMP_RS03640 0.75 -1.4 684,686 - MMP_RS03640 0.76 -1.5 684,860 + -1.2 684,860 + -1.3 684,862 - -0.5 684,875 + -1.0 684,893 + -0.8 684,943 - +0.5 684,944 + +0.0 684,944 + +0.2 684,966 + -3.6 684,983 + -2.2 685,193 + -1.8 685,699 - -3.2 685,701 - -1.8 685,772 + -2.5
Or see this region's nucleotide sequence