Experiment: Formate McCas no stress control 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS02800 and MMP_RS02805 overlap by 14 nucleotides MMP_RS02805 and MMP_RS02810 are separated by 157 nucleotides MMP_RS02810 and MMP_RS02815 are separated by 114 nucleotides MMP_RS02815 and MMP_RS02820 are separated by 6 nucleotides MMP_RS02820 and MMP_RS02825 are separated by 4 nucleotides
MMP_RS02800: MMP_RS02800 - nucleotidyltransferase domain-containing protein, at 535,522 to 536,139
_RS02800
MMP_RS02805: MMP_RS02805 - HEPN domain-containing protein, at 536,126 to 536,635
_RS02805
MMP_RS02810: MMP_RS02810 - hypothetical protein, at 536,793 to 538,262
_RS02810
MMP_RS02815: MMP_RS02815 - hypothetical protein, at 538,377 to 538,952
_RS02815
MMP_RS02820: MMP_RS02820 - hypothetical protein, at 538,959 to 539,189
_RS02820
MMP_RS02825: MMP_RS02825 - mechanosensitive ion channel family protein, at 539,194 to 540,276
_RS02825
Position (kb)
536
537
538
539 Strain fitness (log2 ratio)
-1
0
1 at 535.998 kb on + strand, within MMP_RS02800 at 536.013 kb on + strand, within MMP_RS02800 at 536.060 kb on - strand, within MMP_RS02800 at 536.144 kb on - strand at 536.318 kb on + strand, within MMP_RS02805 at 536.714 kb on - strand at 536.742 kb on - strand at 536.761 kb on - strand at 536.761 kb on - strand at 536.761 kb on - strand at 536.773 kb on + strand at 536.848 kb on - strand at 536.850 kb on - strand at 536.851 kb on + strand at 537.199 kb on - strand, within MMP_RS02810 at 537.239 kb on + strand, within MMP_RS02810 at 537.242 kb on + strand, within MMP_RS02810 at 537.243 kb on - strand, within MMP_RS02810 at 537.272 kb on - strand, within MMP_RS02810 at 537.273 kb on + strand, within MMP_RS02810 at 537.275 kb on + strand, within MMP_RS02810 at 537.277 kb on + strand, within MMP_RS02810 at 537.277 kb on + strand, within MMP_RS02810 at 537.314 kb on + strand, within MMP_RS02810 at 537.406 kb on - strand, within MMP_RS02810 at 537.450 kb on - strand, within MMP_RS02810 at 537.450 kb on - strand, within MMP_RS02810 at 537.638 kb on + strand, within MMP_RS02810 at 537.705 kb on + strand, within MMP_RS02810 at 537.976 kb on - strand, within MMP_RS02810 at 538.472 kb on - strand, within MMP_RS02815 at 538.847 kb on + strand, within MMP_RS02815 at 538.976 kb on - strand at 538.977 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Formate McCas no stress control 2 remove 535,998 + MMP_RS02800 0.77 -1.6 536,013 + MMP_RS02800 0.79 -1.5 536,060 - MMP_RS02800 0.87 +0.4 536,144 - -0.1 536,318 + MMP_RS02805 0.38 -0.0 536,714 - +0.2 536,742 - +0.6 536,761 - -0.1 536,761 - -0.0 536,761 - -0.0 536,773 + -0.1 536,848 - -0.5 536,850 - -0.2 536,851 + +0.2 537,199 - MMP_RS02810 0.28 -0.9 537,239 + MMP_RS02810 0.30 +1.3 537,242 + MMP_RS02810 0.31 +0.1 537,243 - MMP_RS02810 0.31 +0.0 537,272 - MMP_RS02810 0.33 +0.0 537,273 + MMP_RS02810 0.33 -0.1 537,275 + MMP_RS02810 0.33 -0.3 537,277 + MMP_RS02810 0.33 +0.5 537,277 + MMP_RS02810 0.33 +0.0 537,314 + MMP_RS02810 0.35 +0.4 537,406 - MMP_RS02810 0.42 -0.2 537,450 - MMP_RS02810 0.45 +0.1 537,450 - MMP_RS02810 0.45 +0.1 537,638 + MMP_RS02810 0.57 -0.7 537,705 + MMP_RS02810 0.62 -0.1 537,976 - MMP_RS02810 0.80 +0.8 538,472 - MMP_RS02815 0.16 +0.2 538,847 + MMP_RS02815 0.82 +0.5 538,976 - +0.3 538,977 + -0.2
Or see this region's nucleotide sequence