Experiment: Survival; Treatment=UGA synthetic gw pH4.4; TreatmentDuration=0.5hrs; Outgrowth=LBkan50
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt IAI47_20695 and IAI47_20700 are separated by 9 nucleotides IAI47_20700 and IAI47_20705 are separated by 2 nucleotides IAI47_20705 and IAI47_20710 overlap by 17 nucleotides
IAI47_20695: IAI47_20695 - HesA/MoeB/ThiF family protein, at 387,875 to 388,843
_20695
IAI47_20700: IAI47_20700 - thiazole synthase, at 388,853 to 389,608
_20700
IAI47_20705: IAI47_20705 - sulfur carrier protein ThiS, at 389,611 to 389,808
_20705
IAI47_20710: IAI47_20710 - FAD-dependent oxidoreductase, at 389,792 to 390,736
_20710
Position (kb)
389
390 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 388.612 kb on + strand, within IAI47_20695 at 388.687 kb on - strand, within IAI47_20695 at 388.697 kb on - strand, within IAI47_20695 at 388.898 kb on - strand at 388.898 kb on - strand at 388.898 kb on - strand at 388.898 kb on - strand at 389.081 kb on - strand, within IAI47_20700 at 389.098 kb on + strand, within IAI47_20700 at 389.099 kb on - strand, within IAI47_20700 at 389.131 kb on + strand, within IAI47_20700 at 389.233 kb on + strand, within IAI47_20700 at 389.233 kb on + strand, within IAI47_20700 at 389.235 kb on + strand, within IAI47_20700 at 389.236 kb on - strand, within IAI47_20700 at 389.415 kb on + strand, within IAI47_20700 at 389.416 kb on + strand, within IAI47_20700 at 389.416 kb on + strand, within IAI47_20700 at 389.416 kb on + strand, within IAI47_20700 at 389.450 kb on + strand, within IAI47_20700 at 389.450 kb on + strand, within IAI47_20700 at 389.450 kb on + strand, within IAI47_20700 at 389.500 kb on + strand, within IAI47_20700 at 389.500 kb on + strand, within IAI47_20700 at 389.556 kb on + strand at 389.567 kb on - strand at 389.601 kb on + strand at 389.612 kb on + strand at 389.613 kb on - strand at 389.676 kb on - strand, within IAI47_20705 at 389.793 kb on + strand at 389.794 kb on - strand at 389.881 kb on - strand at 389.940 kb on + strand, within IAI47_20710 at 389.940 kb on + strand, within IAI47_20710 at 389.940 kb on + strand, within IAI47_20710 at 389.940 kb on + strand, within IAI47_20710 at 389.940 kb on + strand, within IAI47_20710 at 390.006 kb on + strand, within IAI47_20710 at 390.006 kb on + strand, within IAI47_20710 at 390.006 kb on + strand, within IAI47_20710 at 390.007 kb on - strand, within IAI47_20710 at 390.007 kb on - strand, within IAI47_20710 at 390.007 kb on - strand, within IAI47_20710 at 390.018 kb on - strand, within IAI47_20710 at 390.018 kb on - strand, within IAI47_20710 at 390.018 kb on - strand, within IAI47_20710 at 390.117 kb on + strand, within IAI47_20710 at 390.118 kb on - strand, within IAI47_20710 at 390.118 kb on - strand, within IAI47_20710 at 390.201 kb on - strand, within IAI47_20710 at 390.280 kb on + strand, within IAI47_20710 at 390.281 kb on + strand, within IAI47_20710 at 390.281 kb on + strand, within IAI47_20710 at 390.282 kb on - strand, within IAI47_20710 at 390.282 kb on - strand, within IAI47_20710 at 390.302 kb on + strand, within IAI47_20710 at 390.302 kb on + strand, within IAI47_20710 at 390.303 kb on - strand, within IAI47_20710 at 390.303 kb on - strand, within IAI47_20710 at 390.303 kb on - strand, within IAI47_20710 at 390.303 kb on - strand, within IAI47_20710 at 390.303 kb on - strand, within IAI47_20710 at 390.363 kb on + strand, within IAI47_20710 at 390.363 kb on + strand, within IAI47_20710 at 390.364 kb on - strand, within IAI47_20710 at 390.467 kb on - strand, within IAI47_20710 at 390.494 kb on - strand, within IAI47_20710 at 390.776 kb on + strand at 390.777 kb on - strand at 390.777 kb on - strand at 390.777 kb on - strand at 390.777 kb on - strand at 390.777 kb on - strand at 390.795 kb on + strand at 390.795 kb on + strand at 390.797 kb on + strand at 390.797 kb on + strand at 390.797 kb on + strand at 390.797 kb on + strand at 390.797 kb on + strand at 390.797 kb on + strand at 390.797 kb on + strand at 390.798 kb on - strand at 390.798 kb on - strand at 390.798 kb on - strand at 390.798 kb on - strand at 390.798 kb on - strand at 390.798 kb on - strand at 390.798 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Survival; Treatment=UGA synthetic gw pH4.4; TreatmentDuration=0.5hrs; Outgrowth=LBkan50 remove 388,612 + IAI47_20695 0.76 -2.1 388,687 - IAI47_20695 0.84 +1.7 388,697 - IAI47_20695 0.85 -3.0 388,898 - -0.2 388,898 - +2.5 388,898 - -0.3 388,898 - +1.6 389,081 - IAI47_20700 0.30 +0.8 389,098 + IAI47_20700 0.32 -0.0 389,099 - IAI47_20700 0.33 -0.1 389,131 + IAI47_20700 0.37 -0.9 389,233 + IAI47_20700 0.50 -0.2 389,233 + IAI47_20700 0.50 -3.0 389,235 + IAI47_20700 0.51 +2.6 389,236 - IAI47_20700 0.51 -0.1 389,415 + IAI47_20700 0.74 +0.1 389,416 + IAI47_20700 0.74 +1.6 389,416 + IAI47_20700 0.74 -0.6 389,416 + IAI47_20700 0.74 +0.5 389,450 + IAI47_20700 0.79 -3.3 389,450 + IAI47_20700 0.79 +0.4 389,450 + IAI47_20700 0.79 +1.2 389,500 + IAI47_20700 0.86 -2.2 389,500 + IAI47_20700 0.86 -1.2 389,556 + -0.1 389,567 - -3.1 389,601 + -2.0 389,612 + -0.2 389,613 - +1.0 389,676 - IAI47_20705 0.33 -1.5 389,793 + +1.4 389,794 - +1.3 389,881 - +0.3 389,940 + IAI47_20710 0.16 +0.9 389,940 + IAI47_20710 0.16 -0.6 389,940 + IAI47_20710 0.16 +2.6 389,940 + IAI47_20710 0.16 +0.1 389,940 + IAI47_20710 0.16 +1.1 390,006 + IAI47_20710 0.23 -1.6 390,006 + IAI47_20710 0.23 -1.2 390,006 + IAI47_20710 0.23 -2.0 390,007 - IAI47_20710 0.23 -0.2 390,007 - IAI47_20710 0.23 -0.7 390,007 - IAI47_20710 0.23 +0.1 390,018 - IAI47_20710 0.24 +1.8 390,018 - IAI47_20710 0.24 +0.9 390,018 - IAI47_20710 0.24 -1.0 390,117 + IAI47_20710 0.34 -0.6 390,118 - IAI47_20710 0.34 -1.4 390,118 - IAI47_20710 0.34 -1.4 390,201 - IAI47_20710 0.43 -1.6 390,280 + IAI47_20710 0.52 -0.0 390,281 + IAI47_20710 0.52 -0.4 390,281 + IAI47_20710 0.52 +0.1 390,282 - IAI47_20710 0.52 -2.2 390,282 - IAI47_20710 0.52 -1.8 390,302 + IAI47_20710 0.54 -0.2 390,302 + IAI47_20710 0.54 +2.3 390,303 - IAI47_20710 0.54 -0.5 390,303 - IAI47_20710 0.54 +2.6 390,303 - IAI47_20710 0.54 -1.8 390,303 - IAI47_20710 0.54 +0.4 390,303 - IAI47_20710 0.54 +1.8 390,363 + IAI47_20710 0.60 +0.0 390,363 + IAI47_20710 0.60 -1.2 390,364 - IAI47_20710 0.61 -1.2 390,467 - IAI47_20710 0.71 -1.0 390,494 - IAI47_20710 0.74 +1.0 390,776 + -1.0 390,777 - -1.1 390,777 - +1.0 390,777 - -1.3 390,777 - -0.9 390,777 - +0.4 390,795 + +0.7 390,795 + -2.1 390,797 + +0.0 390,797 + +1.1 390,797 + -1.4 390,797 + +0.1 390,797 + -2.1 390,797 + -0.3 390,797 + +2.2 390,798 - -0.6 390,798 - -2.0 390,798 - +0.7 390,798 - +0.1 390,798 - +2.7 390,798 - +1.2 390,798 - -0.2
Or see this region's nucleotide sequence