Experiment: Survival; Treatment=UGA synthetic gw pH4.4; TreatmentDuration=0.5hrs; Outgrowth=LBkan50
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt IAI47_20515 and IAI47_20520 are separated by 43 nucleotides IAI47_20520 and IAI47_20525 are separated by 362 nucleotides
IAI47_20515: IAI47_20515 - maltoporin, at 351,904 to 353,205
_20515
IAI47_20520: IAI47_20520 - maltose/maltodextrin ABC transporter ATP-binding protein MalK, at 353,249 to 354,358
_20520
IAI47_20525: IAI47_20525 - maltose/maltodextrin ABC transporter substrate-binding protein MalE, at 354,721 to 355,911
_20525
Position (kb)
353
354
355 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 352.293 kb on - strand, within IAI47_20515 at 352.293 kb on - strand, within IAI47_20515 at 352.304 kb on + strand, within IAI47_20515 at 352.304 kb on + strand, within IAI47_20515 at 352.304 kb on + strand, within IAI47_20515 at 352.304 kb on + strand, within IAI47_20515 at 352.304 kb on + strand, within IAI47_20515 at 352.304 kb on + strand, within IAI47_20515 at 352.304 kb on + strand, within IAI47_20515 at 352.304 kb on + strand, within IAI47_20515 at 352.304 kb on + strand, within IAI47_20515 at 352.305 kb on - strand, within IAI47_20515 at 352.305 kb on - strand, within IAI47_20515 at 352.305 kb on - strand, within IAI47_20515 at 352.305 kb on - strand, within IAI47_20515 at 352.305 kb on - strand, within IAI47_20515 at 352.305 kb on - strand, within IAI47_20515 at 352.358 kb on + strand, within IAI47_20515 at 352.374 kb on + strand, within IAI47_20515 at 352.375 kb on - strand, within IAI47_20515 at 352.375 kb on - strand, within IAI47_20515 at 352.434 kb on - strand, within IAI47_20515 at 352.434 kb on - strand, within IAI47_20515 at 352.434 kb on - strand, within IAI47_20515 at 352.454 kb on + strand, within IAI47_20515 at 352.454 kb on + strand, within IAI47_20515 at 352.455 kb on - strand, within IAI47_20515 at 352.455 kb on - strand, within IAI47_20515 at 352.476 kb on + strand, within IAI47_20515 at 352.477 kb on - strand, within IAI47_20515 at 352.477 kb on - strand, within IAI47_20515 at 352.477 kb on - strand, within IAI47_20515 at 352.529 kb on + strand, within IAI47_20515 at 352.556 kb on + strand, within IAI47_20515 at 352.556 kb on + strand, within IAI47_20515 at 352.557 kb on - strand, within IAI47_20515 at 352.557 kb on - strand, within IAI47_20515 at 352.657 kb on - strand, within IAI47_20515 at 352.722 kb on + strand, within IAI47_20515 at 352.770 kb on + strand, within IAI47_20515 at 352.771 kb on - strand, within IAI47_20515 at 352.778 kb on + strand, within IAI47_20515 at 352.779 kb on - strand, within IAI47_20515 at 352.781 kb on + strand, within IAI47_20515 at 352.781 kb on + strand, within IAI47_20515 at 352.781 kb on + strand, within IAI47_20515 at 352.782 kb on - strand, within IAI47_20515 at 352.782 kb on - strand, within IAI47_20515 at 352.782 kb on - strand, within IAI47_20515 at 352.814 kb on + strand, within IAI47_20515 at 352.814 kb on + strand, within IAI47_20515 at 352.881 kb on + strand, within IAI47_20515 at 352.938 kb on - strand, within IAI47_20515 at 352.958 kb on + strand, within IAI47_20515 at 352.958 kb on + strand, within IAI47_20515 at 352.959 kb on - strand, within IAI47_20515 at 352.959 kb on - strand, within IAI47_20515 at 352.959 kb on - strand, within IAI47_20515 at 352.959 kb on - strand, within IAI47_20515 at 352.997 kb on - strand, within IAI47_20515 at 353.012 kb on + strand, within IAI47_20515 at 353.013 kb on - strand, within IAI47_20515 at 353.039 kb on + strand, within IAI47_20515 at 353.040 kb on - strand, within IAI47_20515 at 353.040 kb on - strand, within IAI47_20515 at 353.043 kb on + strand, within IAI47_20515 at 353.043 kb on + strand, within IAI47_20515 at 353.043 kb on + strand, within IAI47_20515 at 353.043 kb on + strand, within IAI47_20515 at 353.043 kb on + strand, within IAI47_20515 at 353.043 kb on + strand, within IAI47_20515 at 353.043 kb on + strand, within IAI47_20515 at 353.043 kb on + strand, within IAI47_20515 at 353.044 kb on - strand, within IAI47_20515 at 353.044 kb on - strand, within IAI47_20515 at 353.044 kb on - strand, within IAI47_20515 at 353.125 kb on - strand at 353.200 kb on + strand at 353.201 kb on - strand at 353.232 kb on + strand at 353.251 kb on - strand at 353.269 kb on - strand at 353.380 kb on - strand, within IAI47_20520 at 353.524 kb on + strand, within IAI47_20520 at 353.524 kb on + strand, within IAI47_20520 at 353.524 kb on + strand, within IAI47_20520 at 353.524 kb on + strand, within IAI47_20520 at 353.524 kb on + strand, within IAI47_20520 at 353.524 kb on + strand, within IAI47_20520 at 353.525 kb on - strand, within IAI47_20520 at 353.525 kb on - strand, within IAI47_20520 at 353.525 kb on - strand, within IAI47_20520 at 353.525 kb on - strand, within IAI47_20520 at 353.525 kb on - strand, within IAI47_20520 at 353.525 kb on - strand, within IAI47_20520 at 353.525 kb on - strand, within IAI47_20520 at 353.525 kb on - strand, within IAI47_20520 at 353.525 kb on - strand, within IAI47_20520 at 353.525 kb on - strand, within IAI47_20520 at 353.525 kb on - strand, within IAI47_20520 at 353.592 kb on - strand, within IAI47_20520 at 353.660 kb on - strand, within IAI47_20520 at 353.688 kb on + strand, within IAI47_20520 at 353.694 kb on + strand, within IAI47_20520 at 353.793 kb on + strand, within IAI47_20520 at 353.794 kb on - strand, within IAI47_20520 at 353.794 kb on - strand, within IAI47_20520 at 353.819 kb on - strand, within IAI47_20520 at 354.074 kb on - strand, within IAI47_20520 at 354.074 kb on - strand, within IAI47_20520 at 354.074 kb on - strand, within IAI47_20520 at 354.100 kb on - strand, within IAI47_20520 at 354.109 kb on - strand, within IAI47_20520 at 354.109 kb on - strand, within IAI47_20520 at 354.109 kb on - strand, within IAI47_20520 at 354.109 kb on - strand, within IAI47_20520 at 354.260 kb on + strand at 354.260 kb on + strand at 354.260 kb on + strand at 354.261 kb on - strand at 354.283 kb on + strand at 354.295 kb on + strand at 354.295 kb on + strand at 354.296 kb on - strand at 354.341 kb on + strand at 354.341 kb on + strand at 354.342 kb on - strand at 354.373 kb on + strand at 354.373 kb on + strand at 354.373 kb on + strand at 354.373 kb on + strand at 354.373 kb on + strand at 354.373 kb on + strand at 354.374 kb on - strand at 354.381 kb on + strand at 354.381 kb on + strand at 354.381 kb on + strand at 354.381 kb on + strand at 354.382 kb on - strand at 354.422 kb on + strand at 354.422 kb on + strand at 354.422 kb on + strand at 354.422 kb on + strand at 354.422 kb on + strand at 354.423 kb on - strand at 354.577 kb on + strand at 354.578 kb on - strand at 354.578 kb on - strand at 354.589 kb on + strand at 354.635 kb on + strand at 354.636 kb on - strand at 354.636 kb on - strand at 354.667 kb on + strand at 354.668 kb on - strand at 354.721 kb on - strand at 354.721 kb on - strand at 354.847 kb on + strand, within IAI47_20525 at 354.848 kb on - strand, within IAI47_20525 at 354.897 kb on + strand, within IAI47_20525 at 354.898 kb on - strand, within IAI47_20525 at 354.898 kb on - strand, within IAI47_20525 at 354.937 kb on + strand, within IAI47_20525 at 354.937 kb on + strand, within IAI47_20525 at 354.975 kb on + strand, within IAI47_20525 at 354.975 kb on + strand, within IAI47_20525 at 354.975 kb on + strand, within IAI47_20525 at 354.976 kb on - strand, within IAI47_20525 at 354.976 kb on - strand, within IAI47_20525 at 355.102 kb on - strand, within IAI47_20525 at 355.148 kb on - strand, within IAI47_20525 at 355.148 kb on - strand, within IAI47_20525 at 355.206 kb on + strand, within IAI47_20525 at 355.207 kb on - strand, within IAI47_20525 at 355.240 kb on - strand, within IAI47_20525 at 355.240 kb on - strand, within IAI47_20525 at 355.240 kb on - strand, within IAI47_20525 at 355.261 kb on + strand, within IAI47_20525 at 355.261 kb on + strand, within IAI47_20525 at 355.261 kb on + strand, within IAI47_20525 at 355.262 kb on - strand, within IAI47_20525 at 355.262 kb on - strand, within IAI47_20525 at 355.262 kb on - strand, within IAI47_20525 at 355.262 kb on - strand, within IAI47_20525 at 355.297 kb on + strand, within IAI47_20525 at 355.297 kb on + strand, within IAI47_20525 at 355.298 kb on - strand, within IAI47_20525 at 355.324 kb on + strand, within IAI47_20525
Per-strain Table
Position Strand Gene LocusTag Fraction Survival; Treatment=UGA synthetic gw pH4.4; TreatmentDuration=0.5hrs; Outgrowth=LBkan50 remove 352,293 - IAI47_20515 0.30 -0.7 352,293 - IAI47_20515 0.30 -0.6 352,304 + IAI47_20515 0.31 -0.3 352,304 + IAI47_20515 0.31 -0.0 352,304 + IAI47_20515 0.31 -2.5 352,304 + IAI47_20515 0.31 -0.2 352,304 + IAI47_20515 0.31 -2.9 352,304 + IAI47_20515 0.31 +0.5 352,304 + IAI47_20515 0.31 +1.7 352,304 + IAI47_20515 0.31 +0.4 352,304 + IAI47_20515 0.31 +1.6 352,305 - IAI47_20515 0.31 +0.9 352,305 - IAI47_20515 0.31 -0.1 352,305 - IAI47_20515 0.31 -1.1 352,305 - IAI47_20515 0.31 -2.2 352,305 - IAI47_20515 0.31 +1.8 352,305 - IAI47_20515 0.31 +0.6 352,358 + IAI47_20515 0.35 -0.8 352,374 + IAI47_20515 0.36 -1.2 352,375 - IAI47_20515 0.36 +0.5 352,375 - IAI47_20515 0.36 -1.5 352,434 - IAI47_20515 0.41 -0.0 352,434 - IAI47_20515 0.41 +0.6 352,434 - IAI47_20515 0.41 -1.6 352,454 + IAI47_20515 0.42 -0.9 352,454 + IAI47_20515 0.42 -1.0 352,455 - IAI47_20515 0.42 +0.7 352,455 - IAI47_20515 0.42 -2.2 352,476 + IAI47_20515 0.44 +1.7 352,477 - IAI47_20515 0.44 +1.1 352,477 - IAI47_20515 0.44 +0.0 352,477 - IAI47_20515 0.44 +0.4 352,529 + IAI47_20515 0.48 +0.8 352,556 + IAI47_20515 0.50 -1.2 352,556 + IAI47_20515 0.50 +0.4 352,557 - IAI47_20515 0.50 +0.6 352,557 - IAI47_20515 0.50 +0.0 352,657 - IAI47_20515 0.58 +0.3 352,722 + IAI47_20515 0.63 +0.6 352,770 + IAI47_20515 0.67 -1.0 352,771 - IAI47_20515 0.67 -0.7 352,778 + IAI47_20515 0.67 -1.0 352,779 - IAI47_20515 0.67 -1.6 352,781 + IAI47_20515 0.67 -0.6 352,781 + IAI47_20515 0.67 -1.0 352,781 + IAI47_20515 0.67 -0.6 352,782 - IAI47_20515 0.67 -0.5 352,782 - IAI47_20515 0.67 +0.1 352,782 - IAI47_20515 0.67 -1.4 352,814 + IAI47_20515 0.70 +1.1 352,814 + IAI47_20515 0.70 -0.6 352,881 + IAI47_20515 0.75 -1.4 352,938 - IAI47_20515 0.79 -1.1 352,958 + IAI47_20515 0.81 -0.8 352,958 + IAI47_20515 0.81 +1.3 352,959 - IAI47_20515 0.81 -1.6 352,959 - IAI47_20515 0.81 -1.6 352,959 - IAI47_20515 0.81 -0.3 352,959 - IAI47_20515 0.81 +0.2 352,997 - IAI47_20515 0.84 -0.4 353,012 + IAI47_20515 0.85 +1.3 353,013 - IAI47_20515 0.85 +0.4 353,039 + IAI47_20515 0.87 +2.0 353,040 - IAI47_20515 0.87 -1.2 353,040 - IAI47_20515 0.87 +0.4 353,043 + IAI47_20515 0.87 -2.1 353,043 + IAI47_20515 0.87 +1.4 353,043 + IAI47_20515 0.87 +0.5 353,043 + IAI47_20515 0.87 -0.2 353,043 + IAI47_20515 0.87 -0.5 353,043 + IAI47_20515 0.87 -2.3 353,043 + IAI47_20515 0.87 -0.1 353,043 + IAI47_20515 0.87 +1.5 353,044 - IAI47_20515 0.88 -2.2 353,044 - IAI47_20515 0.88 -0.5 353,044 - IAI47_20515 0.88 -2.0 353,125 - +2.0 353,200 + -0.3 353,201 - -3.2 353,232 + -1.0 353,251 - -1.6 353,269 - +2.0 353,380 - IAI47_20520 0.12 -1.1 353,524 + IAI47_20520 0.25 -0.4 353,524 + IAI47_20520 0.25 -1.4 353,524 + IAI47_20520 0.25 +0.0 353,524 + IAI47_20520 0.25 -2.6 353,524 + IAI47_20520 0.25 -2.3 353,524 + IAI47_20520 0.25 +0.7 353,525 - IAI47_20520 0.25 -1.0 353,525 - IAI47_20520 0.25 +0.4 353,525 - IAI47_20520 0.25 -2.7 353,525 - IAI47_20520 0.25 +0.0 353,525 - IAI47_20520 0.25 -1.8 353,525 - IAI47_20520 0.25 +0.5 353,525 - IAI47_20520 0.25 -1.2 353,525 - IAI47_20520 0.25 -1.4 353,525 - IAI47_20520 0.25 -2.2 353,525 - IAI47_20520 0.25 +2.2 353,525 - IAI47_20520 0.25 +0.7 353,592 - IAI47_20520 0.31 +0.9 353,660 - IAI47_20520 0.37 +1.5 353,688 + IAI47_20520 0.40 -1.8 353,694 + IAI47_20520 0.40 -1.8 353,793 + IAI47_20520 0.49 +1.5 353,794 - IAI47_20520 0.49 +0.4 353,794 - IAI47_20520 0.49 -2.1 353,819 - IAI47_20520 0.51 +1.4 354,074 - IAI47_20520 0.74 -0.4 354,074 - IAI47_20520 0.74 +0.3 354,074 - IAI47_20520 0.74 -2.2 354,100 - IAI47_20520 0.77 -0.0 354,109 - IAI47_20520 0.77 +1.0 354,109 - IAI47_20520 0.77 -0.4 354,109 - IAI47_20520 0.77 +1.1 354,109 - IAI47_20520 0.77 -1.1 354,260 + +0.8 354,260 + -0.9 354,260 + -1.2 354,261 - -0.8 354,283 + -0.5 354,295 + -2.2 354,295 + -0.8 354,296 - +0.6 354,341 + +0.2 354,341 + -1.0 354,342 - +0.5 354,373 + +0.1 354,373 + -1.6 354,373 + +0.6 354,373 + +1.4 354,373 + +0.6 354,373 + -0.7 354,374 - -1.2 354,381 + -2.4 354,381 + -0.9 354,381 + +0.7 354,381 + +0.8 354,382 - +0.8 354,422 + +2.4 354,422 + +0.5 354,422 + +0.9 354,422 + +1.7 354,422 + -0.7 354,423 - +0.4 354,577 + -0.7 354,578 - +0.4 354,578 - +1.4 354,589 + +0.4 354,635 + -0.8 354,636 - -2.3 354,636 - -0.4 354,667 + -2.7 354,668 - -1.4 354,721 - +1.9 354,721 - +0.7 354,847 + IAI47_20525 0.11 -2.4 354,848 - IAI47_20525 0.11 -1.8 354,897 + IAI47_20525 0.15 -1.2 354,898 - IAI47_20525 0.15 -0.1 354,898 - IAI47_20525 0.15 +2.0 354,937 + IAI47_20525 0.18 +1.7 354,937 + IAI47_20525 0.18 -0.2 354,975 + IAI47_20525 0.21 +0.4 354,975 + IAI47_20525 0.21 +2.0 354,975 + IAI47_20525 0.21 +3.0 354,976 - IAI47_20525 0.21 -1.8 354,976 - IAI47_20525 0.21 +1.4 355,102 - IAI47_20525 0.32 -2.4 355,148 - IAI47_20525 0.36 -1.0 355,148 - IAI47_20525 0.36 +0.6 355,206 + IAI47_20525 0.41 -0.3 355,207 - IAI47_20525 0.41 +3.1 355,240 - IAI47_20525 0.44 -1.1 355,240 - IAI47_20525 0.44 +1.4 355,240 - IAI47_20525 0.44 +1.4 355,261 + IAI47_20525 0.45 -1.7 355,261 + IAI47_20525 0.45 -2.0 355,261 + IAI47_20525 0.45 -1.4 355,262 - IAI47_20525 0.45 -0.6 355,262 - IAI47_20525 0.45 +1.3 355,262 - IAI47_20525 0.45 -0.5 355,262 - IAI47_20525 0.45 -0.5 355,297 + IAI47_20525 0.48 -0.8 355,297 + IAI47_20525 0.48 +0.2 355,298 - IAI47_20525 0.48 +0.4 355,324 + IAI47_20525 0.51 -1.2
Or see this region's nucleotide sequence