Experiment: Formate McCas no stress control 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS06765 and MMP_RS06770 are separated by 36 nucleotides MMP_RS06770 and MMP_RS06775 are separated by 320 nucleotides MMP_RS06775 and MMP_RS06780 are separated by 9 nucleotides
MMP_RS06765: MMP_RS06765 - flap endonuclease-1, at 1,297,500 to 1,298,474
_RS06765
MMP_RS06770: MMP_RS06770 - DHH family phosphoesterase, at 1,298,511 to 1,300,796
_RS06770
MMP_RS06775: MMP_RS06775 - 2-oxoacid:ferredoxin oxidoreductase subunit gamma, at 1,301,117 to 1,301,650
_RS06775
MMP_RS06780: MMP_RS06780 - 2-oxoacid:ferredoxin oxidoreductase subunit beta, at 1,301,660 to 1,302,457
_RS06780
Position (kb)
1298
1299
1300
1301 Strain fitness (log2 ratio)
-2
-1
0
1 at 1297.650 kb on - strand, within MMP_RS06765 at 1297.706 kb on + strand, within MMP_RS06765 at 1297.965 kb on - strand, within MMP_RS06765 at 1297.966 kb on + strand, within MMP_RS06765 at 1297.966 kb on + strand, within MMP_RS06765 at 1297.967 kb on - strand, within MMP_RS06765 at 1298.034 kb on - strand, within MMP_RS06765 at 1298.034 kb on - strand, within MMP_RS06765 at 1298.035 kb on + strand, within MMP_RS06765 at 1298.038 kb on - strand, within MMP_RS06765 at 1298.411 kb on - strand at 1298.661 kb on - strand at 1298.661 kb on - strand at 1298.661 kb on - strand at 1298.661 kb on - strand at 1298.662 kb on + strand at 1298.662 kb on + strand at 1298.662 kb on + strand at 1298.663 kb on - strand at 1299.163 kb on - strand, within MMP_RS06770 at 1299.249 kb on - strand, within MMP_RS06770 at 1299.546 kb on - strand, within MMP_RS06770 at 1299.581 kb on - strand, within MMP_RS06770 at 1299.581 kb on - strand, within MMP_RS06770 at 1299.775 kb on + strand, within MMP_RS06770 at 1299.841 kb on + strand, within MMP_RS06770 at 1299.901 kb on + strand, within MMP_RS06770 at 1299.980 kb on - strand, within MMP_RS06770 at 1299.981 kb on + strand, within MMP_RS06770 at 1300.091 kb on - strand, within MMP_RS06770 at 1300.100 kb on - strand, within MMP_RS06770 at 1300.110 kb on + strand, within MMP_RS06770 at 1300.150 kb on + strand, within MMP_RS06770 at 1300.461 kb on - strand, within MMP_RS06770 at 1300.623 kb on + strand at 1300.797 kb on + strand at 1300.799 kb on - strand at 1300.836 kb on + strand at 1300.878 kb on - strand at 1300.879 kb on + strand at 1300.881 kb on + strand at 1300.882 kb on - strand at 1300.884 kb on - strand at 1300.884 kb on - strand at 1300.958 kb on + strand at 1300.960 kb on + strand at 1301.108 kb on - strand at 1301.444 kb on + strand at 1301.588 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Formate McCas no stress control 2 remove 1,297,650 - MMP_RS06765 0.15 +0.2 1,297,706 + MMP_RS06765 0.21 -0.4 1,297,965 - MMP_RS06765 0.48 +1.3 1,297,966 + MMP_RS06765 0.48 +0.0 1,297,966 + MMP_RS06765 0.48 -0.5 1,297,967 - MMP_RS06765 0.48 +0.3 1,298,034 - MMP_RS06765 0.55 -0.4 1,298,034 - MMP_RS06765 0.55 -0.1 1,298,035 + MMP_RS06765 0.55 -0.3 1,298,038 - MMP_RS06765 0.55 -0.2 1,298,411 - -0.5 1,298,661 - +0.3 1,298,661 - +0.2 1,298,661 - +0.8 1,298,661 - +0.4 1,298,662 + +0.4 1,298,662 + +0.3 1,298,662 + -0.1 1,298,663 - +0.4 1,299,163 - MMP_RS06770 0.29 +0.9 1,299,249 - MMP_RS06770 0.32 +0.2 1,299,546 - MMP_RS06770 0.45 +0.1 1,299,581 - MMP_RS06770 0.47 +1.1 1,299,581 - MMP_RS06770 0.47 +0.4 1,299,775 + MMP_RS06770 0.55 +0.6 1,299,841 + MMP_RS06770 0.58 +0.1 1,299,901 + MMP_RS06770 0.61 -1.9 1,299,980 - MMP_RS06770 0.64 +0.1 1,299,981 + MMP_RS06770 0.64 +0.1 1,300,091 - MMP_RS06770 0.69 +0.0 1,300,100 - MMP_RS06770 0.70 +0.2 1,300,110 + MMP_RS06770 0.70 +0.0 1,300,150 + MMP_RS06770 0.72 +0.4 1,300,461 - MMP_RS06770 0.85 +0.5 1,300,623 + +0.9 1,300,797 + +0.1 1,300,799 - +0.1 1,300,836 + -0.1 1,300,878 - +0.7 1,300,879 + -0.3 1,300,881 + +0.7 1,300,882 - +0.1 1,300,884 - -0.1 1,300,884 - +0.2 1,300,958 + +0.4 1,300,960 + +0.0 1,301,108 - -0.0 1,301,444 + -2.0 1,301,588 - -1.2
Or see this region's nucleotide sequence