Experiment: Formate McCas no stress control 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS05170 and MMP_RS05175 overlap by 4 nucleotides MMP_RS05175 and MMP_RS05180 are separated by 213 nucleotides MMP_RS05180 and MMP_RS05185 are separated by 26 nucleotides MMP_RS05185 and MMP_RS05190 are separated by 0 nucleotides
MMP_RS05170: MMP_RS05170 - lysine exporter LysO family protein, at 984,278 to 984,865
_RS05170
MMP_RS05175: MMP_RS05175 - hypothetical protein, at 984,862 to 985,110
_RS05175
MMP_RS05180: MMP_RS05180 - Nre family DNA repair protein, at 985,324 to 986,544
_RS05180
MMP_RS05185: MMP_RS05185 - tryptophan synthase subunit alpha, at 986,571 to 987,347
_RS05185
MMP_RS05190: MMP_RS05190 - tryptophan synthase subunit beta, at 987,348 to 988,532
_RS05190
Position (kb)
985
986
987 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 984.362 kb on - strand, within MMP_RS05170 at 984.478 kb on - strand, within MMP_RS05170 at 984.479 kb on + strand, within MMP_RS05170 at 984.479 kb on + strand, within MMP_RS05170 at 984.540 kb on - strand, within MMP_RS05170 at 984.667 kb on - strand, within MMP_RS05170 at 984.667 kb on - strand, within MMP_RS05170 at 984.766 kb on + strand, within MMP_RS05170 at 984.766 kb on + strand, within MMP_RS05170 at 984.805 kb on - strand, within MMP_RS05170 at 984.820 kb on - strand at 984.821 kb on + strand at 984.873 kb on - strand at 984.906 kb on - strand, within MMP_RS05175 at 984.914 kb on + strand, within MMP_RS05175 at 984.940 kb on - strand, within MMP_RS05175 at 984.941 kb on + strand, within MMP_RS05175 at 984.941 kb on + strand, within MMP_RS05175 at 985.079 kb on + strand, within MMP_RS05175 at 985.096 kb on + strand at 985.097 kb on - strand at 985.098 kb on + strand at 985.098 kb on + strand at 985.110 kb on - strand at 985.142 kb on - strand at 985.158 kb on + strand at 985.159 kb on - strand at 985.162 kb on - strand at 985.162 kb on - strand at 985.164 kb on - strand at 985.165 kb on + strand at 985.165 kb on + strand at 985.166 kb on - strand at 985.167 kb on + strand at 985.175 kb on + strand at 985.175 kb on + strand at 985.186 kb on - strand at 985.187 kb on + strand at 985.189 kb on + strand at 985.244 kb on + strand at 985.244 kb on + strand at 985.247 kb on - strand at 985.247 kb on - strand at 985.247 kb on - strand at 985.247 kb on - strand at 985.251 kb on - strand at 985.252 kb on + strand at 985.254 kb on - strand at 985.254 kb on - strand at 985.323 kb on - strand at 985.400 kb on - strand at 985.401 kb on + strand at 985.446 kb on - strand, within MMP_RS05180 at 985.801 kb on + strand, within MMP_RS05180 at 985.852 kb on - strand, within MMP_RS05180 at 985.852 kb on - strand, within MMP_RS05180 at 985.921 kb on - strand, within MMP_RS05180 at 985.924 kb on - strand, within MMP_RS05180 at 985.925 kb on + strand, within MMP_RS05180 at 985.925 kb on + strand, within MMP_RS05180 at 985.994 kb on - strand, within MMP_RS05180 at 986.075 kb on + strand, within MMP_RS05180 at 986.097 kb on - strand, within MMP_RS05180 at 986.101 kb on - strand, within MMP_RS05180 at 986.102 kb on + strand, within MMP_RS05180 at 986.128 kb on - strand, within MMP_RS05180 at 986.270 kb on - strand, within MMP_RS05180 at 986.321 kb on - strand, within MMP_RS05180 at 986.321 kb on - strand, within MMP_RS05180 at 986.533 kb on + strand at 986.572 kb on - strand at 986.632 kb on + strand at 986.679 kb on - strand at 987.015 kb on + strand at 987.183 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Formate McCas no stress control 2 remove 984,362 - MMP_RS05170 0.14 +0.3 984,478 - MMP_RS05170 0.34 -0.2 984,479 + MMP_RS05170 0.34 -0.4 984,479 + MMP_RS05170 0.34 +0.0 984,540 - MMP_RS05170 0.45 +1.1 984,667 - MMP_RS05170 0.66 +0.4 984,667 - MMP_RS05170 0.66 +0.4 984,766 + MMP_RS05170 0.83 +0.1 984,766 + MMP_RS05170 0.83 +0.3 984,805 - MMP_RS05170 0.90 +0.0 984,820 - -0.1 984,821 + +0.6 984,873 - -0.0 984,906 - MMP_RS05175 0.18 +0.2 984,914 + MMP_RS05175 0.21 +0.5 984,940 - MMP_RS05175 0.31 -0.1 984,941 + MMP_RS05175 0.32 +0.4 984,941 + MMP_RS05175 0.32 -0.2 985,079 + MMP_RS05175 0.87 -0.2 985,096 + +0.2 985,097 - -0.2 985,098 + +0.5 985,098 + -0.1 985,110 - -0.0 985,142 - +0.1 985,158 + +0.6 985,159 - +0.0 985,162 - +0.2 985,162 - +0.1 985,164 - +0.0 985,165 + +0.0 985,165 + -0.7 985,166 - -0.5 985,167 + -0.2 985,175 + +0.1 985,175 + +0.1 985,186 - -0.1 985,187 + +0.4 985,189 + +0.5 985,244 + -0.1 985,244 + +0.5 985,247 - -0.0 985,247 - -0.6 985,247 - +0.1 985,247 - +0.3 985,251 - +0.8 985,252 + +0.0 985,254 - -0.2 985,254 - +0.5 985,323 - -0.3 985,400 - +0.5 985,401 + +0.4 985,446 - MMP_RS05180 0.10 +0.3 985,801 + MMP_RS05180 0.39 +0.3 985,852 - MMP_RS05180 0.43 +0.9 985,852 - MMP_RS05180 0.43 +1.0 985,921 - MMP_RS05180 0.49 +0.1 985,924 - MMP_RS05180 0.49 -0.1 985,925 + MMP_RS05180 0.49 +0.0 985,925 + MMP_RS05180 0.49 +0.2 985,994 - MMP_RS05180 0.55 -0.2 986,075 + MMP_RS05180 0.62 -0.0 986,097 - MMP_RS05180 0.63 +0.4 986,101 - MMP_RS05180 0.64 -0.1 986,102 + MMP_RS05180 0.64 +0.7 986,128 - MMP_RS05180 0.66 +0.0 986,270 - MMP_RS05180 0.77 +0.4 986,321 - MMP_RS05180 0.82 -0.1 986,321 - MMP_RS05180 0.82 -0.1 986,533 + -0.1 986,572 - +0.0 986,632 + -2.1 986,679 - -2.3 987,015 + -3.8 987,183 - -1.7
Or see this region's nucleotide sequence