Experiment: Formate McCas no stress control 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS03985 and MMP_RS03990 are separated by 944 nucleotides MMP_RS03990 and MMP_RS03995 are separated by 163 nucleotides
MMP_RS03985: MMP_RS03985 - hypothetical protein, at 754,505 to 755,509
_RS03985
MMP_RS03990: MMP_RS03990 - recombinase family protein, at 756,454 to 757,008
_RS03990
MMP_RS03995: MMP_RS03995 - flippase-like domain-containing protein, at 757,172 to 758,122
_RS03995
Position (kb)
756
757
758 Strain fitness (log2 ratio)
-1
0
1 at 755.454 kb on - strand at 755.498 kb on + strand at 755.512 kb on - strand at 755.643 kb on - strand at 755.645 kb on - strand at 755.645 kb on - strand at 755.646 kb on + strand at 755.648 kb on + strand at 755.648 kb on + strand at 755.648 kb on + strand at 755.665 kb on - strand at 755.708 kb on - strand at 755.708 kb on - strand at 755.709 kb on + strand at 755.709 kb on + strand at 755.709 kb on + strand at 755.709 kb on + strand at 755.710 kb on - strand at 755.711 kb on + strand at 755.794 kb on - strand at 755.836 kb on - strand at 755.836 kb on - strand at 755.836 kb on - strand at 755.837 kb on + strand at 755.837 kb on + strand at 755.859 kb on - strand at 755.860 kb on + strand at 755.892 kb on + strand at 755.937 kb on + strand at 755.937 kb on + strand at 755.955 kb on - strand at 755.956 kb on + strand at 755.989 kb on - strand at 755.990 kb on + strand at 755.991 kb on - strand at 755.995 kb on - strand at 755.996 kb on + strand at 755.998 kb on - strand at 755.998 kb on - strand at 756.074 kb on - strand at 756.093 kb on - strand at 756.093 kb on - strand at 756.109 kb on - strand at 756.118 kb on + strand at 756.143 kb on - strand at 756.147 kb on - strand at 756.148 kb on + strand at 756.203 kb on + strand at 756.209 kb on + strand at 756.267 kb on - strand at 756.305 kb on + strand at 756.339 kb on + strand at 756.371 kb on + strand at 756.391 kb on - strand at 756.392 kb on + strand at 756.455 kb on - strand at 756.455 kb on - strand at 756.456 kb on + strand at 756.473 kb on - strand at 756.477 kb on - strand at 756.653 kb on + strand, within MMP_RS03990 at 756.768 kb on - strand, within MMP_RS03990 at 756.835 kb on + strand, within MMP_RS03990 at 756.837 kb on - strand, within MMP_RS03990 at 756.840 kb on - strand, within MMP_RS03990 at 756.853 kb on - strand, within MMP_RS03990 at 756.894 kb on - strand, within MMP_RS03990 at 756.894 kb on - strand, within MMP_RS03990 at 756.895 kb on + strand, within MMP_RS03990 at 756.895 kb on + strand, within MMP_RS03990 at 756.942 kb on - strand, within MMP_RS03990 at 756.981 kb on - strand at 756.982 kb on + strand at 756.982 kb on + strand at 757.008 kb on - strand at 757.008 kb on - strand at 757.009 kb on + strand at 757.057 kb on - strand at 757.081 kb on - strand at 757.081 kb on - strand at 757.082 kb on + strand at 757.166 kb on + strand at 757.166 kb on + strand at 757.226 kb on + strand at 757.374 kb on + strand, within MMP_RS03995 at 757.374 kb on + strand, within MMP_RS03995 at 757.399 kb on - strand, within MMP_RS03995 at 757.434 kb on - strand, within MMP_RS03995 at 757.434 kb on - strand, within MMP_RS03995 at 757.434 kb on - strand, within MMP_RS03995 at 757.435 kb on + strand, within MMP_RS03995 at 757.435 kb on + strand, within MMP_RS03995 at 757.435 kb on + strand, within MMP_RS03995 at 757.441 kb on - strand, within MMP_RS03995 at 757.555 kb on - strand, within MMP_RS03995 at 757.616 kb on - strand, within MMP_RS03995 at 757.671 kb on + strand, within MMP_RS03995 at 757.672 kb on - strand, within MMP_RS03995 at 757.673 kb on + strand, within MMP_RS03995 at 757.727 kb on - strand, within MMP_RS03995 at 757.797 kb on + strand, within MMP_RS03995 at 758.006 kb on + strand, within MMP_RS03995
Per-strain Table
Position Strand Gene LocusTag Fraction Formate McCas no stress control 2 remove 755,454 - +0.8 755,498 + -0.3 755,512 - -0.2 755,643 - +0.2 755,645 - +0.5 755,645 - -0.0 755,646 + +0.2 755,648 + -0.5 755,648 + -0.2 755,648 + +0.4 755,665 - -0.2 755,708 - +0.2 755,708 - +0.6 755,709 + +0.2 755,709 + -0.2 755,709 + +0.1 755,709 + +0.6 755,710 - -0.3 755,711 + +0.4 755,794 - +0.2 755,836 - -0.1 755,836 - +0.1 755,836 - -0.3 755,837 + +0.5 755,837 + -0.3 755,859 - +0.2 755,860 + -0.1 755,892 + -0.1 755,937 + +0.4 755,937 + -0.1 755,955 - +0.3 755,956 + +0.8 755,989 - +0.1 755,990 + -0.4 755,991 - -0.1 755,995 - -0.1 755,996 + +0.1 755,998 - +0.1 755,998 - -0.3 756,074 - +0.3 756,093 - -0.0 756,093 - +0.5 756,109 - +0.0 756,118 + +0.1 756,143 - -0.2 756,147 - -0.3 756,148 + +0.1 756,203 + +0.8 756,209 + -0.2 756,267 - +0.5 756,305 + -0.3 756,339 + +0.5 756,371 + -0.3 756,391 - +0.1 756,392 + -0.2 756,455 - +0.3 756,455 - -0.6 756,456 + -0.3 756,473 - +0.5 756,477 - -0.0 756,653 + MMP_RS03990 0.36 +0.1 756,768 - MMP_RS03990 0.57 +0.0 756,835 + MMP_RS03990 0.69 +0.4 756,837 - MMP_RS03990 0.69 +0.2 756,840 - MMP_RS03990 0.70 +0.1 756,853 - MMP_RS03990 0.72 +0.5 756,894 - MMP_RS03990 0.79 +0.3 756,894 - MMP_RS03990 0.79 +0.1 756,895 + MMP_RS03990 0.79 -0.5 756,895 + MMP_RS03990 0.79 -0.3 756,942 - MMP_RS03990 0.88 +0.8 756,981 - -0.3 756,982 + -0.0 756,982 + +0.3 757,008 - -0.2 757,008 - -0.1 757,009 + +0.0 757,057 - -0.4 757,081 - +0.2 757,081 - -0.2 757,082 + +0.2 757,166 + -0.5 757,166 + -0.1 757,226 + -1.0 757,374 + MMP_RS03995 0.21 -0.5 757,374 + MMP_RS03995 0.21 -0.1 757,399 - MMP_RS03995 0.24 -0.1 757,434 - MMP_RS03995 0.28 -0.0 757,434 - MMP_RS03995 0.28 -0.3 757,434 - MMP_RS03995 0.28 +0.0 757,435 + MMP_RS03995 0.28 -0.1 757,435 + MMP_RS03995 0.28 -0.0 757,435 + MMP_RS03995 0.28 -0.1 757,441 - MMP_RS03995 0.28 +0.3 757,555 - MMP_RS03995 0.40 +0.2 757,616 - MMP_RS03995 0.47 +0.5 757,671 + MMP_RS03995 0.52 -0.4 757,672 - MMP_RS03995 0.53 +0.3 757,673 + MMP_RS03995 0.53 +0.2 757,727 - MMP_RS03995 0.58 +0.3 757,797 + MMP_RS03995 0.66 -0.2 758,006 + MMP_RS03995 0.88 +0.1
Or see this region's nucleotide sequence