Experiment: Formate McCas no stress control 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS02300 and MMP_RS02305 are separated by 38 nucleotides MMP_RS02305 and MMP_RS02310 are separated by 266 nucleotides MMP_RS02310 and MMP_RS02315 are separated by 0 nucleotides MMP_RS02315 and MMP_RS02320 overlap by 14 nucleotides
MMP_RS02300: MMP_RS02300 - NAD(P)H-dependent oxidoreductase, at 433,982 to 434,476
_RS02300
MMP_RS02305: MMP_RS02305 - DUF378 domain-containing protein, at 434,515 to 434,763
_RS02305
MMP_RS02310: MMP_RS02310 - RimK-like ATPgrasp N-terminal domain-containing protein, at 435,030 to 435,635
_RS02310
MMP_RS02315: MMP_RS02315 - RimK family alpha-L-glutamate ligase, at 435,636 to 436,586
_RS02315
MMP_RS02320: MMP_RS02320 - glutamate-cysteine ligase family protein, at 436,573 to 437,676
_RS02320
Position (kb)
435
436 Strain fitness (log2 ratio)
-1
0
1 at 434.033 kb on - strand, within MMP_RS02300 at 434.199 kb on + strand, within MMP_RS02300 at 434.207 kb on + strand, within MMP_RS02300 at 434.208 kb on - strand, within MMP_RS02300 at 434.332 kb on - strand, within MMP_RS02300 at 434.449 kb on - strand at 434.449 kb on - strand at 434.456 kb on - strand at 434.457 kb on + strand at 434.457 kb on + strand at 434.474 kb on - strand at 434.532 kb on + strand at 434.535 kb on + strand at 434.543 kb on - strand, within MMP_RS02305 at 434.543 kb on - strand, within MMP_RS02305 at 434.543 kb on - strand, within MMP_RS02305 at 434.543 kb on - strand, within MMP_RS02305 at 434.543 kb on - strand, within MMP_RS02305 at 434.543 kb on - strand, within MMP_RS02305 at 434.543 kb on - strand, within MMP_RS02305 at 434.543 kb on - strand, within MMP_RS02305 at 434.543 kb on - strand, within MMP_RS02305 at 434.544 kb on + strand, within MMP_RS02305 at 434.544 kb on + strand, within MMP_RS02305 at 434.544 kb on + strand, within MMP_RS02305 at 434.572 kb on - strand, within MMP_RS02305 at 434.653 kb on + strand, within MMP_RS02305 at 434.665 kb on - strand, within MMP_RS02305 at 434.679 kb on - strand, within MMP_RS02305 at 434.680 kb on + strand, within MMP_RS02305 at 434.680 kb on + strand, within MMP_RS02305 at 434.728 kb on + strand, within MMP_RS02305 at 434.803 kb on - strand at 434.803 kb on - strand at 435.312 kb on - strand, within MMP_RS02310 at 435.922 kb on + strand, within MMP_RS02315 at 436.008 kb on + strand, within MMP_RS02315 at 436.109 kb on + strand, within MMP_RS02315 at 436.278 kb on - strand, within MMP_RS02315 at 436.326 kb on + strand, within MMP_RS02315 at 436.408 kb on + strand, within MMP_RS02315 at 436.473 kb on - strand, within MMP_RS02315 at 436.491 kb on - strand, within MMP_RS02315 at 436.492 kb on + strand at 436.494 kb on + strand at 436.518 kb on - strand at 436.518 kb on - strand at 436.594 kb on - strand at 436.595 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Formate McCas no stress control 2 remove 434,033 - MMP_RS02300 0.10 +0.8 434,199 + MMP_RS02300 0.44 -0.3 434,207 + MMP_RS02300 0.45 -0.3 434,208 - MMP_RS02300 0.46 +0.2 434,332 - MMP_RS02300 0.71 -0.0 434,449 - -0.2 434,449 - -0.1 434,456 - +0.2 434,457 + -0.1 434,457 + -0.3 434,474 - -0.3 434,532 + -0.1 434,535 + -0.6 434,543 - MMP_RS02305 0.11 +0.1 434,543 - MMP_RS02305 0.11 -0.2 434,543 - MMP_RS02305 0.11 -0.2 434,543 - MMP_RS02305 0.11 +0.1 434,543 - MMP_RS02305 0.11 -0.0 434,543 - MMP_RS02305 0.11 +0.0 434,543 - MMP_RS02305 0.11 -0.2 434,543 - MMP_RS02305 0.11 -0.3 434,543 - MMP_RS02305 0.11 -0.2 434,544 + MMP_RS02305 0.12 -0.0 434,544 + MMP_RS02305 0.12 +0.1 434,544 + MMP_RS02305 0.12 +0.9 434,572 - MMP_RS02305 0.23 -0.0 434,653 + MMP_RS02305 0.55 -0.0 434,665 - MMP_RS02305 0.60 +0.1 434,679 - MMP_RS02305 0.66 -0.6 434,680 + MMP_RS02305 0.66 +0.1 434,680 + MMP_RS02305 0.66 -0.2 434,728 + MMP_RS02305 0.86 -0.4 434,803 - +0.3 434,803 - -0.5 435,312 - MMP_RS02310 0.47 +0.2 435,922 + MMP_RS02315 0.30 -0.0 436,008 + MMP_RS02315 0.39 -0.2 436,109 + MMP_RS02315 0.50 -0.3 436,278 - MMP_RS02315 0.68 -0.1 436,326 + MMP_RS02315 0.73 +0.3 436,408 + MMP_RS02315 0.81 +0.2 436,473 - MMP_RS02315 0.88 +0.6 436,491 - MMP_RS02315 0.90 +0.6 436,492 + +0.4 436,494 + +0.1 436,518 - -0.6 436,518 - +0.5 436,594 - -0.4 436,595 + -0.1
Or see this region's nucleotide sequence