Experiment: LB no stress 48 hours
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2037 and Pf6N2E2_2038 are separated by 105 nucleotides Pf6N2E2_2038 and Pf6N2E2_2039 overlap by 1 nucleotides Pf6N2E2_2039 and Pf6N2E2_2040 are separated by 302 nucleotides
Pf6N2E2_2037 - Thioredoxin reductase (EC 1.8.1.9), at 2,425,811 to 2,426,704
_2037
Pf6N2E2_2038 - Transcriptional regulator, TetR family, at 2,426,810 to 2,427,469
_2038
Pf6N2E2_2039 - Sigma-54 dependent transcriptional regulator, at 2,427,469 to 2,428,884
_2039
Pf6N2E2_2040 - D-beta-hydroxybutyrate permease, at 2,429,187 to 2,430,578
_2040
Position (kb)
2427
2428
2429 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2426.677 kb on + strand at 2426.869 kb on + strand at 2426.870 kb on - strand at 2426.870 kb on - strand at 2426.870 kb on - strand at 2426.870 kb on - strand at 2426.870 kb on - strand at 2426.872 kb on - strand at 2426.872 kb on - strand at 2427.197 kb on + strand, within Pf6N2E2_2038 at 2427.197 kb on + strand, within Pf6N2E2_2038 at 2427.215 kb on + strand, within Pf6N2E2_2038 at 2427.215 kb on + strand, within Pf6N2E2_2038 at 2427.216 kb on - strand, within Pf6N2E2_2038 at 2427.393 kb on - strand, within Pf6N2E2_2038 at 2427.393 kb on - strand, within Pf6N2E2_2038 at 2427.393 kb on - strand, within Pf6N2E2_2038 at 2427.393 kb on - strand, within Pf6N2E2_2038 at 2427.496 kb on + strand at 2427.496 kb on + strand at 2427.610 kb on + strand at 2427.610 kb on + strand at 2427.862 kb on + strand, within Pf6N2E2_2039 at 2427.925 kb on + strand, within Pf6N2E2_2039 at 2427.926 kb on - strand, within Pf6N2E2_2039 at 2428.228 kb on - strand, within Pf6N2E2_2039 at 2428.306 kb on + strand, within Pf6N2E2_2039 at 2428.417 kb on + strand, within Pf6N2E2_2039 at 2428.553 kb on + strand, within Pf6N2E2_2039 at 2428.554 kb on - strand, within Pf6N2E2_2039 at 2428.554 kb on - strand, within Pf6N2E2_2039 at 2428.677 kb on - strand, within Pf6N2E2_2039 at 2428.843 kb on + strand at 2428.844 kb on - strand at 2428.844 kb on - strand at 2428.882 kb on + strand at 2428.904 kb on + strand at 2428.906 kb on + strand at 2428.983 kb on - strand at 2429.167 kb on - strand at 2429.371 kb on - strand, within Pf6N2E2_2040 at 2429.468 kb on + strand, within Pf6N2E2_2040 at 2429.517 kb on + strand, within Pf6N2E2_2040 at 2429.517 kb on + strand, within Pf6N2E2_2040 at 2429.517 kb on + strand, within Pf6N2E2_2040 at 2429.518 kb on - strand, within Pf6N2E2_2040 at 2429.518 kb on - strand, within Pf6N2E2_2040 at 2429.518 kb on - strand, within Pf6N2E2_2040 at 2429.588 kb on + strand, within Pf6N2E2_2040 at 2429.588 kb on + strand, within Pf6N2E2_2040 at 2429.589 kb on - strand, within Pf6N2E2_2040 at 2429.589 kb on - strand, within Pf6N2E2_2040 at 2429.591 kb on + strand, within Pf6N2E2_2040 at 2429.591 kb on + strand, within Pf6N2E2_2040 at 2429.591 kb on + strand, within Pf6N2E2_2040 at 2429.591 kb on + strand, within Pf6N2E2_2040 at 2429.592 kb on - strand, within Pf6N2E2_2040 at 2429.592 kb on - strand, within Pf6N2E2_2040
Per-strain Table
Position Strand Gene LocusTag Fraction LB no stress 48 hours remove 2,426,677 + -0.4 2,426,869 + -0.3 2,426,870 - +0.1 2,426,870 - +0.8 2,426,870 - +0.5 2,426,870 - +0.3 2,426,870 - -0.5 2,426,872 - -0.2 2,426,872 - -0.6 2,427,197 + Pf6N2E2_2038 0.59 -0.7 2,427,197 + Pf6N2E2_2038 0.59 +0.1 2,427,215 + Pf6N2E2_2038 0.61 -0.6 2,427,215 + Pf6N2E2_2038 0.61 -1.1 2,427,216 - Pf6N2E2_2038 0.62 +0.6 2,427,393 - Pf6N2E2_2038 0.88 -0.6 2,427,393 - Pf6N2E2_2038 0.88 -0.2 2,427,393 - Pf6N2E2_2038 0.88 -0.7 2,427,393 - Pf6N2E2_2038 0.88 -0.5 2,427,496 + +1.8 2,427,496 + -0.3 2,427,610 + +0.6 2,427,610 + -0.2 2,427,862 + Pf6N2E2_2039 0.28 -0.2 2,427,925 + Pf6N2E2_2039 0.32 -0.7 2,427,926 - Pf6N2E2_2039 0.32 -2.2 2,428,228 - Pf6N2E2_2039 0.54 -0.7 2,428,306 + Pf6N2E2_2039 0.59 +0.0 2,428,417 + Pf6N2E2_2039 0.67 +0.2 2,428,553 + Pf6N2E2_2039 0.77 -1.6 2,428,554 - Pf6N2E2_2039 0.77 -0.5 2,428,554 - Pf6N2E2_2039 0.77 -1.3 2,428,677 - Pf6N2E2_2039 0.85 +0.9 2,428,843 + -1.1 2,428,844 - +0.9 2,428,844 - -0.1 2,428,882 + +0.0 2,428,904 + -0.3 2,428,906 + -1.3 2,428,983 - -0.7 2,429,167 - -0.2 2,429,371 - Pf6N2E2_2040 0.13 -1.5 2,429,468 + Pf6N2E2_2040 0.20 -0.1 2,429,517 + Pf6N2E2_2040 0.24 +1.1 2,429,517 + Pf6N2E2_2040 0.24 +0.5 2,429,517 + Pf6N2E2_2040 0.24 +0.3 2,429,518 - Pf6N2E2_2040 0.24 +1.3 2,429,518 - Pf6N2E2_2040 0.24 +0.2 2,429,518 - Pf6N2E2_2040 0.24 -1.7 2,429,588 + Pf6N2E2_2040 0.29 -0.1 2,429,588 + Pf6N2E2_2040 0.29 -0.8 2,429,589 - Pf6N2E2_2040 0.29 +0.1 2,429,589 - Pf6N2E2_2040 0.29 -0.7 2,429,591 + Pf6N2E2_2040 0.29 +0.4 2,429,591 + Pf6N2E2_2040 0.29 +0.1 2,429,591 + Pf6N2E2_2040 0.29 +0.6 2,429,591 + Pf6N2E2_2040 0.29 -1.0 2,429,592 - Pf6N2E2_2040 0.29 -0.2 2,429,592 - Pf6N2E2_2040 0.29 -1.5
Or see this region's nucleotide sequence