Experiment: LB no stress 48 hours
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1038 and Pf6N2E2_1039 are separated by 105 nucleotides Pf6N2E2_1039 and Pf6N2E2_1040 are separated by 328 nucleotides
Pf6N2E2_1038 - citrate transporter, at 1,211,420 to 1,212,706
_1038
Pf6N2E2_1039 - Gluconolactonase, at 1,212,812 to 1,213,738
_1039
Pf6N2E2_1040 - Hydroxymethylglutaryl-CoA lyase (EC 4.1.3.4), at 1,214,067 to 1,215,008
_1040
Position (kb)
1212
1213
1214 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1212.140 kb on + strand, within Pf6N2E2_1038 at 1212.140 kb on + strand, within Pf6N2E2_1038 at 1212.286 kb on + strand, within Pf6N2E2_1038 at 1212.286 kb on + strand, within Pf6N2E2_1038 at 1212.286 kb on + strand, within Pf6N2E2_1038 at 1212.287 kb on - strand, within Pf6N2E2_1038 at 1212.354 kb on + strand, within Pf6N2E2_1038 at 1212.354 kb on + strand, within Pf6N2E2_1038 at 1212.354 kb on + strand, within Pf6N2E2_1038 at 1212.354 kb on + strand, within Pf6N2E2_1038 at 1212.354 kb on + strand, within Pf6N2E2_1038 at 1212.354 kb on + strand, within Pf6N2E2_1038 at 1212.354 kb on + strand, within Pf6N2E2_1038 at 1212.354 kb on + strand, within Pf6N2E2_1038 at 1212.354 kb on + strand, within Pf6N2E2_1038 at 1212.355 kb on - strand, within Pf6N2E2_1038 at 1212.355 kb on - strand, within Pf6N2E2_1038 at 1212.355 kb on - strand, within Pf6N2E2_1038 at 1212.355 kb on - strand, within Pf6N2E2_1038 at 1212.355 kb on - strand, within Pf6N2E2_1038 at 1212.355 kb on - strand, within Pf6N2E2_1038 at 1212.355 kb on - strand, within Pf6N2E2_1038 at 1212.355 kb on - strand, within Pf6N2E2_1038 at 1212.355 kb on - strand, within Pf6N2E2_1038 at 1212.359 kb on - strand, within Pf6N2E2_1038 at 1212.629 kb on + strand at 1212.663 kb on + strand at 1212.663 kb on + strand at 1212.663 kb on + strand at 1212.664 kb on - strand at 1212.687 kb on + strand at 1212.814 kb on - strand at 1212.911 kb on - strand, within Pf6N2E2_1039 at 1213.027 kb on - strand, within Pf6N2E2_1039 at 1213.261 kb on - strand, within Pf6N2E2_1039 at 1213.266 kb on + strand, within Pf6N2E2_1039 at 1213.317 kb on + strand, within Pf6N2E2_1039 at 1213.318 kb on - strand, within Pf6N2E2_1039 at 1213.318 kb on - strand, within Pf6N2E2_1039 at 1213.500 kb on + strand, within Pf6N2E2_1039 at 1213.500 kb on + strand, within Pf6N2E2_1039 at 1213.572 kb on + strand, within Pf6N2E2_1039 at 1213.572 kb on + strand, within Pf6N2E2_1039 at 1213.729 kb on - strand at 1213.769 kb on + strand at 1213.770 kb on - strand at 1213.789 kb on + strand at 1213.789 kb on + strand at 1213.951 kb on + strand at 1213.951 kb on + strand at 1213.951 kb on + strand at 1213.951 kb on + strand at 1213.951 kb on + strand at 1213.951 kb on + strand at 1213.951 kb on + strand at 1213.951 kb on + strand at 1213.951 kb on + strand at 1213.951 kb on + strand at 1213.951 kb on + strand at 1213.951 kb on + strand at 1213.951 kb on + strand at 1213.951 kb on + strand at 1213.951 kb on + strand at 1213.951 kb on + strand at 1213.951 kb on + strand at 1213.951 kb on + strand at 1213.951 kb on + strand at 1213.951 kb on + strand at 1213.951 kb on + strand at 1213.951 kb on + strand at 1213.951 kb on + strand at 1213.951 kb on + strand at 1213.951 kb on + strand at 1213.951 kb on + strand at 1213.951 kb on + strand at 1213.951 kb on + strand at 1213.952 kb on - strand at 1213.952 kb on - strand at 1213.952 kb on - strand at 1213.952 kb on - strand at 1213.952 kb on - strand at 1213.952 kb on - strand at 1213.952 kb on - strand at 1213.952 kb on - strand at 1213.952 kb on - strand at 1213.952 kb on - strand at 1213.952 kb on - strand at 1213.952 kb on - strand at 1213.952 kb on - strand at 1213.952 kb on - strand at 1213.952 kb on - strand at 1213.952 kb on - strand at 1213.952 kb on - strand at 1213.952 kb on - strand at 1213.952 kb on - strand at 1213.952 kb on - strand at 1213.952 kb on - strand at 1213.952 kb on - strand at 1213.952 kb on - strand at 1213.952 kb on - strand at 1213.952 kb on - strand at 1214.075 kb on + strand at 1214.076 kb on - strand at 1214.076 kb on - strand at 1214.086 kb on + strand at 1214.086 kb on + strand at 1214.086 kb on + strand at 1214.086 kb on + strand at 1214.086 kb on + strand at 1214.086 kb on + strand at 1214.086 kb on + strand at 1214.087 kb on - strand at 1214.087 kb on - strand at 1214.087 kb on - strand at 1214.090 kb on + strand at 1214.090 kb on + strand at 1214.091 kb on - strand at 1214.091 kb on - strand at 1214.091 kb on - strand at 1214.366 kb on + strand, within Pf6N2E2_1040 at 1214.513 kb on - strand, within Pf6N2E2_1040
Per-strain Table
Position Strand Gene LocusTag Fraction LB no stress 48 hours remove 1,212,140 + Pf6N2E2_1038 0.56 +0.7 1,212,140 + Pf6N2E2_1038 0.56 -0.2 1,212,286 + Pf6N2E2_1038 0.67 -0.0 1,212,286 + Pf6N2E2_1038 0.67 +0.2 1,212,286 + Pf6N2E2_1038 0.67 +0.4 1,212,287 - Pf6N2E2_1038 0.67 -1.9 1,212,354 + Pf6N2E2_1038 0.73 -0.9 1,212,354 + Pf6N2E2_1038 0.73 +0.6 1,212,354 + Pf6N2E2_1038 0.73 +0.3 1,212,354 + Pf6N2E2_1038 0.73 -0.5 1,212,354 + Pf6N2E2_1038 0.73 +0.6 1,212,354 + Pf6N2E2_1038 0.73 +0.3 1,212,354 + Pf6N2E2_1038 0.73 +0.9 1,212,354 + Pf6N2E2_1038 0.73 +1.8 1,212,354 + Pf6N2E2_1038 0.73 +3.6 1,212,355 - Pf6N2E2_1038 0.73 +0.5 1,212,355 - Pf6N2E2_1038 0.73 -0.2 1,212,355 - Pf6N2E2_1038 0.73 +0.3 1,212,355 - Pf6N2E2_1038 0.73 -0.4 1,212,355 - Pf6N2E2_1038 0.73 +1.3 1,212,355 - Pf6N2E2_1038 0.73 +0.8 1,212,355 - Pf6N2E2_1038 0.73 +1.4 1,212,355 - Pf6N2E2_1038 0.73 +0.4 1,212,355 - Pf6N2E2_1038 0.73 +0.8 1,212,359 - Pf6N2E2_1038 0.73 +1.1 1,212,629 + -0.3 1,212,663 + +0.1 1,212,663 + +0.6 1,212,663 + +0.9 1,212,664 - +0.3 1,212,687 + -1.0 1,212,814 - +2.2 1,212,911 - Pf6N2E2_1039 0.11 -0.6 1,213,027 - Pf6N2E2_1039 0.23 -2.5 1,213,261 - Pf6N2E2_1039 0.48 -0.4 1,213,266 + Pf6N2E2_1039 0.49 +0.1 1,213,317 + Pf6N2E2_1039 0.54 -0.4 1,213,318 - Pf6N2E2_1039 0.55 -0.4 1,213,318 - Pf6N2E2_1039 0.55 +0.3 1,213,500 + Pf6N2E2_1039 0.74 -1.3 1,213,500 + Pf6N2E2_1039 0.74 -0.0 1,213,572 + Pf6N2E2_1039 0.82 -0.8 1,213,572 + Pf6N2E2_1039 0.82 -0.2 1,213,729 - +0.1 1,213,769 + -0.3 1,213,770 - +2.4 1,213,789 + +0.2 1,213,789 + -0.1 1,213,951 + +0.4 1,213,951 + -0.7 1,213,951 + -0.6 1,213,951 + -0.0 1,213,951 + +0.5 1,213,951 + -0.2 1,213,951 + -0.5 1,213,951 + +0.4 1,213,951 + -1.8 1,213,951 + -1.1 1,213,951 + -0.0 1,213,951 + -0.5 1,213,951 + -3.0 1,213,951 + -0.4 1,213,951 + +2.2 1,213,951 + +0.5 1,213,951 + +0.2 1,213,951 + -0.8 1,213,951 + -0.2 1,213,951 + -0.6 1,213,951 + +0.2 1,213,951 + -0.1 1,213,951 + -1.1 1,213,951 + -1.5 1,213,951 + -0.6 1,213,951 + -0.3 1,213,951 + -0.7 1,213,951 + +0.2 1,213,952 - -1.0 1,213,952 - -0.2 1,213,952 - +0.6 1,213,952 - -0.8 1,213,952 - +0.5 1,213,952 - +0.3 1,213,952 - -1.1 1,213,952 - -0.9 1,213,952 - -0.1 1,213,952 - +0.1 1,213,952 - +0.2 1,213,952 - -1.1 1,213,952 - +0.0 1,213,952 - -0.7 1,213,952 - +1.5 1,213,952 - -0.5 1,213,952 - -0.5 1,213,952 - +0.7 1,213,952 - +1.3 1,213,952 - -0.2 1,213,952 - -1.5 1,213,952 - +0.7 1,213,952 - -0.2 1,213,952 - -1.1 1,213,952 - +1.9 1,214,075 + -0.4 1,214,076 - -0.8 1,214,076 - +0.7 1,214,086 + -0.6 1,214,086 + +1.1 1,214,086 + -0.5 1,214,086 + +0.4 1,214,086 + +0.2 1,214,086 + +0.4 1,214,086 + +0.3 1,214,087 - +1.1 1,214,087 - +1.8 1,214,087 - -0.3 1,214,090 + -1.1 1,214,090 + +0.2 1,214,091 - +0.8 1,214,091 - +0.2 1,214,091 - +0.1 1,214,366 + Pf6N2E2_1040 0.32 -1.5 1,214,513 - Pf6N2E2_1040 0.47 -0.2
Or see this region's nucleotide sequence