Experiment: L-Serine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5614 and Pf1N1B4_5615 overlap by 4 nucleotides Pf1N1B4_5615 and Pf1N1B4_5616 are separated by 61 nucleotides Pf1N1B4_5616 and Pf1N1B4_6052 are separated by 168 nucleotides Pf1N1B4_6052 and Pf1N1B4_5617 are separated by 63 nucleotides Pf1N1B4_5617 and Pf1N1B4_5618 are separated by 33 nucleotides
Pf1N1B4_5614 - COGs COG3777, at 40,025 to 40,867
_5614
Pf1N1B4_5615 - Citrate lyase beta chain (EC 4.1.3.6), at 40,864 to 41,679
_5615
Pf1N1B4_5616 - Succinate-semialdehyde dehydrogenase [NAD] (EC 1.2.1.24); Succinate-semialdehyde dehydrogenase [NAD(P)+] (EC 1.2.1.16), at 41,741 to 43,132
_5616
Pf1N1B4_6052 - tRNA-Gly-GCC, at 43,301 to 43,373
_6052
Pf1N1B4_5617 - CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase (EC 2.7.8.5), at 43,437 to 43,997
_5617
Pf1N1B4_5618 - Excinuclease ABC subunit C, at 44,031 to 45,854
_5618
Position (kb) 41
42
43
44 Strain fitness (log2 ratio) -2
-1
0
1
2 at 41.433 kb on + strand, within Pf1N1B4_5615 at 41.682 kb on - strand at 41.807 kb on + strand at 41.931 kb on + strand, within Pf1N1B4_5616 at 42.212 kb on + strand, within Pf1N1B4_5616 at 42.212 kb on + strand, within Pf1N1B4_5616 at 42.212 kb on + strand, within Pf1N1B4_5616 at 42.213 kb on - strand, within Pf1N1B4_5616 at 42.213 kb on - strand, within Pf1N1B4_5616 at 42.251 kb on + strand, within Pf1N1B4_5616 at 42.659 kb on + strand, within Pf1N1B4_5616 at 42.659 kb on + strand at 42.659 kb on + strand, within Pf1N1B4_5616 at 42.660 kb on - strand, within Pf1N1B4_5616 at 42.660 kb on - strand, within Pf1N1B4_5616 at 42.687 kb on + strand, within Pf1N1B4_5616 at 42.936 kb on - strand, within Pf1N1B4_5616 at 43.439 kb on - strand at 44.033 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Serine (N) remove 41,433 + Pf1N1B4_5615 0.70 +0.7 41,682 - +0.2 41,807 + -0.1 41,931 + Pf1N1B4_5616 0.14 -0.9 42,212 + Pf1N1B4_5616 0.34 +0.4 42,212 + Pf1N1B4_5616 0.34 -0.2 42,212 + Pf1N1B4_5616 0.34 +0.6 42,213 - Pf1N1B4_5616 0.34 +0.3 42,213 - Pf1N1B4_5616 0.34 +0.1 42,251 + Pf1N1B4_5616 0.37 +0.2 42,659 + Pf1N1B4_5616 0.66 +0.1 42,659 + +2.7 42,659 + Pf1N1B4_5616 0.66 +0.3 42,660 - Pf1N1B4_5616 0.66 +1.1 42,660 - Pf1N1B4_5616 0.66 -0.6 42,687 + Pf1N1B4_5616 0.68 -1.9 42,936 - Pf1N1B4_5616 0.86 +0.5 43,439 - -1.1 44,033 - +0.2
Or see this region's nucleotide sequence