Experiment: L-Serine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4909 and Pf1N1B4_4910 are separated by 236 nucleotides Pf1N1B4_4910 and Pf1N1B4_4911 are separated by 95 nucleotides Pf1N1B4_4911 and Pf1N1B4_4912 are separated by 45 nucleotides
Pf1N1B4_4909 - D-3-phosphoglycerate dehydrogenase (EC 1.1.1.95), at 5,438,861 to 5,439,823
_4909
Pf1N1B4_4910 - Gamma-aminobutyrate:alpha-ketoglutarate aminotransferase (EC 2.6.1.19), at 5,440,060 to 5,441,352
_4910
Pf1N1B4_4911 - FIG00953249: hypothetical protein, at 5,441,448 to 5,442,104
_4911
Pf1N1B4_4912 - Leucine-responsive regulatory protein, regulator for leucine (or lrp) regulon and high-affinity branched-chain amino acid transport system, at 5,442,150 to 5,442,638
_4912
Position (kb)
5440
5441
5442 Strain fitness (log2 ratio)
-1
0
1 at 5439.495 kb on + strand, within Pf1N1B4_4909 at 5439.663 kb on + strand at 5439.772 kb on + strand at 5439.773 kb on - strand at 5439.775 kb on - strand at 5439.876 kb on + strand at 5439.982 kb on + strand at 5440.319 kb on - strand, within Pf1N1B4_4910 at 5440.327 kb on + strand, within Pf1N1B4_4910 at 5440.380 kb on + strand, within Pf1N1B4_4910 at 5440.534 kb on + strand, within Pf1N1B4_4910 at 5440.534 kb on + strand, within Pf1N1B4_4910 at 5440.536 kb on + strand, within Pf1N1B4_4910 at 5440.536 kb on + strand, within Pf1N1B4_4910 at 5440.537 kb on - strand at 5440.963 kb on + strand, within Pf1N1B4_4910 at 5440.963 kb on + strand, within Pf1N1B4_4910 at 5440.963 kb on + strand, within Pf1N1B4_4910 at 5440.964 kb on - strand, within Pf1N1B4_4910 at 5440.964 kb on - strand, within Pf1N1B4_4910 at 5441.402 kb on - strand at 5441.402 kb on - strand at 5441.441 kb on - strand at 5441.441 kb on - strand at 5441.441 kb on - strand at 5442.045 kb on + strand at 5442.125 kb on - strand at 5442.125 kb on - strand at 5442.161 kb on - strand at 5442.164 kb on - strand at 5442.303 kb on + strand, within Pf1N1B4_4912 at 5442.306 kb on - strand, within Pf1N1B4_4912 at 5442.316 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Serine (N) remove 5,439,495 + Pf1N1B4_4909 0.66 -0.1 5,439,663 + -0.0 5,439,772 + -0.9 5,439,773 - -0.3 5,439,775 - +0.6 5,439,876 + -1.0 5,439,982 + +1.2 5,440,319 - Pf1N1B4_4910 0.20 +0.3 5,440,327 + Pf1N1B4_4910 0.21 -0.0 5,440,380 + Pf1N1B4_4910 0.25 -0.4 5,440,534 + Pf1N1B4_4910 0.37 -0.4 5,440,534 + Pf1N1B4_4910 0.37 -0.3 5,440,536 + Pf1N1B4_4910 0.37 +0.0 5,440,536 + Pf1N1B4_4910 0.37 +0.1 5,440,537 - +1.2 5,440,963 + Pf1N1B4_4910 0.70 -0.1 5,440,963 + Pf1N1B4_4910 0.70 -0.3 5,440,963 + Pf1N1B4_4910 0.70 -0.4 5,440,964 - Pf1N1B4_4910 0.70 +0.6 5,440,964 - Pf1N1B4_4910 0.70 +0.3 5,441,402 - +0.7 5,441,402 - +0.6 5,441,441 - -0.2 5,441,441 - -0.2 5,441,441 - -0.2 5,442,045 + -0.9 5,442,125 - +0.9 5,442,125 - -0.4 5,442,161 - +0.2 5,442,164 - +1.2 5,442,303 + Pf1N1B4_4912 0.31 +0.0 5,442,306 - Pf1N1B4_4912 0.32 +0.2 5,442,316 - +1.0
Or see this region's nucleotide sequence