Experiment: L-Serine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4773 and Pf1N1B4_4774 are separated by 292 nucleotides Pf1N1B4_4774 and Pf1N1B4_4775 are separated by 4 nucleotides Pf1N1B4_4775 and Pf1N1B4_4776 are separated by 194 nucleotides
Pf1N1B4_4773 - RND efflux system, membrane fusion protein CmeA, at 5,274,456 to 5,275,613
_4773
Pf1N1B4_4774 - Transcriptional regulatory protein RstA, at 5,275,906 to 5,276,607
_4774
Pf1N1B4_4775 - Sensory histidine kinase QseC, at 5,276,612 to 5,277,913
_4775
Pf1N1B4_4776 - Nicotinamidase (EC 3.5.1.19), at 5,278,108 to 5,278,746
_4776
Position (kb)
5276
5277
5278 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 5275.615 kb on + strand at 5275.616 kb on - strand at 5275.616 kb on - strand at 5275.616 kb on - strand at 5275.731 kb on + strand at 5275.731 kb on + strand at 5275.732 kb on - strand at 5275.732 kb on - strand at 5275.733 kb on + strand at 5275.768 kb on + strand at 5275.768 kb on + strand at 5275.812 kb on + strand at 5276.383 kb on + strand, within Pf1N1B4_4774 at 5276.383 kb on + strand, within Pf1N1B4_4774 at 5276.384 kb on - strand, within Pf1N1B4_4774 at 5276.424 kb on - strand, within Pf1N1B4_4774 at 5276.424 kb on - strand, within Pf1N1B4_4774 at 5276.637 kb on - strand at 5276.672 kb on + strand at 5276.672 kb on + strand at 5276.673 kb on - strand at 5276.673 kb on - strand at 5276.723 kb on + strand at 5276.723 kb on + strand at 5276.723 kb on + strand at 5276.724 kb on - strand at 5276.724 kb on - strand at 5276.735 kb on + strand at 5277.026 kb on + strand, within Pf1N1B4_4775 at 5277.422 kb on + strand, within Pf1N1B4_4775 at 5277.546 kb on + strand, within Pf1N1B4_4775 at 5277.546 kb on + strand, within Pf1N1B4_4775 at 5277.943 kb on + strand at 5277.991 kb on - strand at 5277.991 kb on - strand at 5278.127 kb on - strand at 5278.307 kb on + strand, within Pf1N1B4_4776 at 5278.307 kb on + strand, within Pf1N1B4_4776 at 5278.308 kb on - strand, within Pf1N1B4_4776 at 5278.308 kb on - strand, within Pf1N1B4_4776 at 5278.308 kb on - strand, within Pf1N1B4_4776 at 5278.308 kb on - strand, within Pf1N1B4_4776 at 5278.388 kb on + strand, within Pf1N1B4_4776 at 5278.477 kb on - strand, within Pf1N1B4_4776 at 5278.539 kb on - strand at 5278.705 kb on + strand at 5278.705 kb on + strand at 5278.706 kb on - strand at 5278.706 kb on - strand at 5278.783 kb on - strand at 5278.783 kb on - strand at 5278.788 kb on + strand at 5278.788 kb on + strand at 5278.789 kb on - strand at 5278.899 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Serine (N) remove 5,275,615 + -1.1 5,275,616 - +0.5 5,275,616 - +0.4 5,275,616 - +0.6 5,275,731 + +0.2 5,275,731 + -0.7 5,275,732 - +0.1 5,275,732 - +0.6 5,275,733 + -1.0 5,275,768 + +1.3 5,275,768 + -0.4 5,275,812 + +0.4 5,276,383 + Pf1N1B4_4774 0.68 +0.1 5,276,383 + Pf1N1B4_4774 0.68 +0.3 5,276,384 - Pf1N1B4_4774 0.68 +0.4 5,276,424 - Pf1N1B4_4774 0.74 +0.2 5,276,424 - Pf1N1B4_4774 0.74 +0.3 5,276,637 - +1.1 5,276,672 + +0.6 5,276,672 + -1.8 5,276,673 - +1.9 5,276,673 - -0.2 5,276,723 + +0.2 5,276,723 + +0.1 5,276,723 + -1.6 5,276,724 - -0.4 5,276,724 - -0.2 5,276,735 + -0.2 5,277,026 + Pf1N1B4_4775 0.32 -1.3 5,277,422 + Pf1N1B4_4775 0.62 -0.4 5,277,546 + Pf1N1B4_4775 0.72 -0.9 5,277,546 + Pf1N1B4_4775 0.72 +0.7 5,277,943 + -0.0 5,277,991 - -0.8 5,277,991 - +0.1 5,278,127 - -0.2 5,278,307 + Pf1N1B4_4776 0.31 -0.4 5,278,307 + Pf1N1B4_4776 0.31 -0.2 5,278,308 - Pf1N1B4_4776 0.31 +0.1 5,278,308 - Pf1N1B4_4776 0.31 -0.7 5,278,308 - Pf1N1B4_4776 0.31 +0.1 5,278,308 - Pf1N1B4_4776 0.31 -0.4 5,278,388 + Pf1N1B4_4776 0.44 -0.8 5,278,477 - Pf1N1B4_4776 0.58 -0.7 5,278,539 - -0.6 5,278,705 + +0.1 5,278,705 + -0.6 5,278,706 - -1.4 5,278,706 - +1.0 5,278,783 - -1.2 5,278,783 - -0.9 5,278,788 + -0.4 5,278,788 + +0.1 5,278,789 - -1.7 5,278,899 + +0.7
Or see this region's nucleotide sequence