Experiment: L-Serine (N) 
Only strains with sufficient reads to estimate fitness are shown,
                   but the strain fitness values are still rather noisy and may be biased towards zero.
                   Strains near the edge of a gene are not shown as being associated with that
                   gene (they are in grey).
                   Strains in the central 10-90% of a gene are color coded by the insertion's strand.
                   Usually, "+" means that the selectable marker is encoded on the forward strand,
                   i.e., transcribed rightward. 
    
        
         
     
    
        
         
     
    
        
         
     
    
        
         
     
    
         
     
     
 
500 nt Pf1N1B4_3589 and Pf1N1B4_3590 are separated by 155 nucleotides Pf1N1B4_3590 and Pf1N1B4_3591 overlap by 18 nucleotides Pf1N1B4_3591 and Pf1N1B4_3592 are separated by 83 nucleotides  
        
        Pf1N1B4_3589 - N-acetylneuraminate synthase (EC 2.5.1.56), at 4,001,835 to 4,002,887 
         
        _3589 
         
        
        Pf1N1B4_3590 - hypothetical protein, at 4,003,043 to 4,003,180 
         
        _3590 
         
        
        Pf1N1B4_3591 - 3-oxoacyl-[acyl-carrier-protein] synthase, KASIII (EC 2.3.1.41), at 4,003,163 to 4,004,044 
         
        _3591 
         
        
        Pf1N1B4_3592 - Glutamate synthase [NADPH] large chain (EC 1.4.1.13), at 4,004,128 to 4,005,420 
         
        _3592 
         Position (kb)  
4003 
 
4004 
 
4005 Strain fitness (log2 ratio)  
-1 
 
0 
 
1 
 
2 at 4002.225 kb on + strand, within Pf1N1B4_3589 at 4002.226 kb on - strand, within Pf1N1B4_3589 at 4002.226 kb on - strand, within Pf1N1B4_3589 at 4002.226 kb on - strand, within Pf1N1B4_3589 at 4002.256 kb on + strand, within Pf1N1B4_3589 at 4002.257 kb on - strand, within Pf1N1B4_3589 at 4002.402 kb on + strand, within Pf1N1B4_3589 at 4002.704 kb on - strand, within Pf1N1B4_3589 at 4002.721 kb on - strand, within Pf1N1B4_3589 at 4002.721 kb on - strand, within Pf1N1B4_3589 at 4002.721 kb on - strand, within Pf1N1B4_3589 at 4002.721 kb on - strand, within Pf1N1B4_3589 at 4002.721 kb on - strand, within Pf1N1B4_3589 at 4002.721 kb on - strand, within Pf1N1B4_3589 at 4002.721 kb on - strand, within Pf1N1B4_3589 at 4002.721 kb on - strand, within Pf1N1B4_3589 at 4002.721 kb on - strand, within Pf1N1B4_3589 at 4002.721 kb on - strand, within Pf1N1B4_3589 at 4002.721 kb on - strand, within Pf1N1B4_3589 at 4002.721 kb on - strand, within Pf1N1B4_3589 at 4002.915 kb on - strand at 4002.934 kb on - strand at 4002.934 kb on - strand at 4002.951 kb on + strand at 4002.952 kb on - strand at 4002.952 kb on - strand at 4002.952 kb on - strand at 4002.952 kb on - strand at 4002.952 kb on - strand at 4002.952 kb on - strand at 4002.957 kb on - strand at 4002.957 kb on - strand at 4002.957 kb on - strand at 4002.957 kb on - strand at 4002.957 kb on - strand at 4002.957 kb on - strand at 4002.957 kb on - strand at 4002.957 kb on - strand at 4002.959 kb on - strand at 4003.009 kb on - strand at 4003.009 kb on - strand at 4003.009 kb on - strand at 4003.045 kb on - strand at 4003.089 kb on - strand, within Pf1N1B4_3590 at 4003.089 kb on - strand, within Pf1N1B4_3590 at 4003.101 kb on - strand, within Pf1N1B4_3590 at 4003.101 kb on - strand, within Pf1N1B4_3590 at 4003.128 kb on + strand, within Pf1N1B4_3590 at 4003.129 kb on - strand, within Pf1N1B4_3590 at 4003.145 kb on + strand, within Pf1N1B4_3590 at 4003.145 kb on + strand, within Pf1N1B4_3590 at 4003.145 kb on + strand, within Pf1N1B4_3590 at 4003.145 kb on + strand, within Pf1N1B4_3590 at 4003.145 kb on + strand, within Pf1N1B4_3590 at 4003.146 kb on - strand, within Pf1N1B4_3590 at 4003.146 kb on - strand, within Pf1N1B4_3590 at 4003.146 kb on - strand, within Pf1N1B4_3590 at 4003.146 kb on - strand, within Pf1N1B4_3590 at 4003.172 kb on + strand at 4003.172 kb on + strand at 4003.172 kb on + strand at 4003.172 kb on + strand at 4003.173 kb on - strand at 4003.173 kb on - strand at 4003.173 kb on - strand at 4003.173 kb on - strand at 4003.173 kb on - strand at 4003.173 kb on - strand at 4003.173 kb on - strand at 4003.173 kb on - strand at 4003.173 kb on - strand at 4003.444 kb on + strand, within Pf1N1B4_3591 at 4003.445 kb on - strand, within Pf1N1B4_3591 at 4003.466 kb on + strand, within Pf1N1B4_3591 at 4003.466 kb on + strand, within Pf1N1B4_3591 at 4003.467 kb on - strand, within Pf1N1B4_3591 at 4003.467 kb on - strand, within Pf1N1B4_3591 at 4003.481 kb on + strand, within Pf1N1B4_3591 at 4003.481 kb on + strand, within Pf1N1B4_3591 at 4003.481 kb on + strand, within Pf1N1B4_3591 at 4003.481 kb on + strand, within Pf1N1B4_3591 at 4003.481 kb on + strand, within Pf1N1B4_3591 at 4003.482 kb on - strand, within Pf1N1B4_3591 at 4003.482 kb on - strand, within Pf1N1B4_3591 at 4003.482 kb on - strand, within Pf1N1B4_3591 at 4003.482 kb on - strand, within Pf1N1B4_3591 at 4003.547 kb on + strand, within Pf1N1B4_3591 at 4003.918 kb on + strand, within Pf1N1B4_3591 at 4003.918 kb on + strand, within Pf1N1B4_3591 at 4004.033 kb on + strand at 4004.033 kb on + strand at 4004.033 kb on + strand at 4004.033 kb on + strand at 4004.033 kb on + strand at 4004.033 kb on + strand at 4004.034 kb on - strand at 4004.034 kb on - strand at 4004.034 kb on - strand at 4004.034 kb on - strand at 4004.034 kb on - strand at 4004.034 kb on - strand at 4004.034 kb on - strand at 4004.034 kb on - strand at 4004.034 kb on - strand at 4004.034 kb on - strand at 4004.034 kb on - strand at 4004.050 kb on + strand at 4004.050 kb on + strand at 4004.050 kb on + strand at 4004.051 kb on - strand at 4004.051 kb on - strand at 4004.083 kb on + strand at 4004.097 kb on - strand at 4004.163 kb on - strand at 4004.163 kb on - strand at 4004.417 kb on + strand, within Pf1N1B4_3592 at 4004.417 kb on + strand, within Pf1N1B4_3592 at 4004.418 kb on - strand, within Pf1N1B4_3592 at 4004.418 kb on - strand, within Pf1N1B4_3592 at 4004.418 kb on - strand, within Pf1N1B4_3592 at 4004.418 kb on - strand at 4004.423 kb on + strand, within Pf1N1B4_3592 at 4004.423 kb on + strand, within Pf1N1B4_3592 at 4004.423 kb on + strand, within Pf1N1B4_3592 at 4004.423 kb on + strand, within Pf1N1B4_3592 at 4004.423 kb on + strand, within Pf1N1B4_3592 at 4004.423 kb on + strand, within Pf1N1B4_3592 at 4004.423 kb on + strand, within Pf1N1B4_3592 at 4004.423 kb on + strand, within Pf1N1B4_3592 at 4004.423 kb on + strand at 4004.424 kb on - strand, within Pf1N1B4_3592 at 4004.424 kb on - strand, within Pf1N1B4_3592 at 4004.424 kb on - strand, within Pf1N1B4_3592 at 4004.424 kb on - strand, within Pf1N1B4_3592 at 4004.424 kb on - strand, within Pf1N1B4_3592 at 4004.424 kb on - strand, within Pf1N1B4_3592 at 4004.424 kb on - strand at 4004.424 kb on - strand, within Pf1N1B4_3592 at 4004.424 kb on - strand, within Pf1N1B4_3592 at 4004.424 kb on - strand, within Pf1N1B4_3592 at 4004.424 kb on - strand, within Pf1N1B4_3592 at 4004.424 kb on - strand, within Pf1N1B4_3592 at 4004.424 kb on - strand, within Pf1N1B4_3592 at 4004.424 kb on - strand, within Pf1N1B4_3592 at 4004.424 kb on - strand, within Pf1N1B4_3592 at 4004.424 kb on - strand, within Pf1N1B4_3592 at 4004.424 kb on - strand, within Pf1N1B4_3592 at 4004.424 kb on - strand, within Pf1N1B4_3592 at 4004.424 kb on - strand, within Pf1N1B4_3592 at 4004.424 kb on - strand, within Pf1N1B4_3592 at 4004.427 kb on + strand, within Pf1N1B4_3592 at 4004.427 kb on + strand at 4004.428 kb on - strand at 4004.428 kb on - strand, within Pf1N1B4_3592 at 4004.512 kb on + strand, within Pf1N1B4_3592 at 4004.513 kb on - strand at 4004.513 kb on - strand, within Pf1N1B4_3592 at 4004.513 kb on - strand, within Pf1N1B4_3592 at 4004.513 kb on - strand, within Pf1N1B4_3592 at 4004.513 kb on - strand, within Pf1N1B4_3592 at 4004.526 kb on - strand, within Pf1N1B4_3592 at 4004.526 kb on - strand, within Pf1N1B4_3592 at 4004.584 kb on + strand, within Pf1N1B4_3592 at 4004.584 kb on + strand, within Pf1N1B4_3592 at 4004.585 kb on - strand, within Pf1N1B4_3592 at 4004.669 kb on + strand, within Pf1N1B4_3592 at 4004.669 kb on + strand, within Pf1N1B4_3592 at 4004.669 kb on + strand, within Pf1N1B4_3592 at 4004.670 kb on - strand at 4004.919 kb on + strand, within Pf1N1B4_3592 at 4004.919 kb on + strand, within Pf1N1B4_3592 at 4004.919 kb on + strand, within Pf1N1B4_3592 at 4004.958 kb on + strand, within Pf1N1B4_3592 at 4004.958 kb on + strand, within Pf1N1B4_3592 at 4004.958 kb on + strand, within Pf1N1B4_3592 at 4004.958 kb on + strand, within Pf1N1B4_3592 at 4004.971 kb on + strand, within Pf1N1B4_3592 at 4004.971 kb on + strand, within Pf1N1B4_3592 at 4004.971 kb on + strand, within Pf1N1B4_3592 at 4004.971 kb on + strand, within Pf1N1B4_3592 at 4004.972 kb on - strand, within Pf1N1B4_3592 at 4004.972 kb on - strand, within Pf1N1B4_3592 at 4004.972 kb on - strand, within Pf1N1B4_3592 at 4004.972 kb on - strand at 4004.972 kb on - strand, within Pf1N1B4_3592 at 4004.972 kb on - strand, within Pf1N1B4_3592 at 4004.972 kb on - strand, within Pf1N1B4_3592 at 4004.972 kb on - strand, within Pf1N1B4_3592 at 4004.974 kb on - strand, within Pf1N1B4_3592 at 4005.022 kb on + strand, within Pf1N1B4_3592 at 4005.022 kb on + strand, within Pf1N1B4_3592 at 4005.022 kb on + strand, within Pf1N1B4_3592 at 4005.022 kb on + strand, within Pf1N1B4_3592 at 4005.023 kb on - strand, within Pf1N1B4_3592  
 
 
 
Per-strain Table 
Position  Strand  Gene  LocusTag  Fraction   L-Serine (N)             remove   4,002,225   +    Pf1N1B4_3589   0.37  +0.2   4,002,226   -    Pf1N1B4_3589   0.37  +0.4   4,002,226   -    Pf1N1B4_3589   0.37  +0.4   4,002,226   -    Pf1N1B4_3589   0.37  -0.1   4,002,256   +    Pf1N1B4_3589   0.40  -0.3   4,002,257   -    Pf1N1B4_3589   0.40  -1.2   4,002,402   +    Pf1N1B4_3589   0.54  -0.1   4,002,704   -    Pf1N1B4_3589   0.83  +0.7   4,002,721   -    Pf1N1B4_3589   0.84  +0.6   4,002,721   -    Pf1N1B4_3589   0.84  +0.7   4,002,721   -    Pf1N1B4_3589   0.84  +0.8   4,002,721   -    Pf1N1B4_3589   0.84  +0.7   4,002,721   -    Pf1N1B4_3589   0.84  +0.3   4,002,721   -    Pf1N1B4_3589   0.84  +0.8   4,002,721   -    Pf1N1B4_3589   0.84  +1.9   4,002,721   -    Pf1N1B4_3589   0.84  +0.5   4,002,721   -    Pf1N1B4_3589   0.84  +1.0   4,002,721   -    Pf1N1B4_3589   0.84  +0.6   4,002,721   -    Pf1N1B4_3589   0.84  +0.3   4,002,721   -    Pf1N1B4_3589   0.84  -0.1   4,002,915   -        +0.7   4,002,934   -        +0.5   4,002,934   -        +0.9   4,002,951   +        -0.2   4,002,952   -        +0.6   4,002,952   -        +0.5   4,002,952   -        +0.8   4,002,952   -        +0.6   4,002,952   -        +0.8   4,002,952   -        +0.8   4,002,957   -        +0.8   4,002,957   -        +0.8   4,002,957   -        +0.9   4,002,957   -        +0.4   4,002,957   -        +0.6   4,002,957   -        +0.9   4,002,957   -        -0.3   4,002,957   -        +1.0   4,002,959   -        +0.6   4,003,009   -        +1.0   4,003,009   -        +1.0   4,003,009   -        +1.0   4,003,045   -        -0.1   4,003,089   -    Pf1N1B4_3590   0.33  +0.4   4,003,089   -    Pf1N1B4_3590   0.33  +0.0   4,003,101   -    Pf1N1B4_3590   0.42  +0.5   4,003,101   -    Pf1N1B4_3590   0.42  +1.0   4,003,128   +    Pf1N1B4_3590   0.62  +0.6   4,003,129   -    Pf1N1B4_3590   0.62  +0.6   4,003,145   +    Pf1N1B4_3590   0.74  +0.5   4,003,145   +    Pf1N1B4_3590   0.74  +0.3   4,003,145   +    Pf1N1B4_3590   0.74  +0.1   4,003,145   +    Pf1N1B4_3590   0.74  +0.4   4,003,145   +    Pf1N1B4_3590   0.74  +0.9   4,003,146   -    Pf1N1B4_3590   0.75  +1.2   4,003,146   -    Pf1N1B4_3590   0.75  +0.5   4,003,146   -    Pf1N1B4_3590   0.75  +0.0   4,003,146   -    Pf1N1B4_3590   0.75  +0.9   4,003,172   +        +1.3   4,003,172   +        -1.2   4,003,172   +        +0.1   4,003,172   +        +0.3   4,003,173   -        +0.8   4,003,173   -        +0.7   4,003,173   -        +0.1   4,003,173   -        +1.0   4,003,173   -        -0.1   4,003,173   -        +0.3   4,003,173   -        +0.4   4,003,173   -        +0.5   4,003,173   -        +0.9   4,003,444   +    Pf1N1B4_3591   0.32  +0.6   4,003,445   -    Pf1N1B4_3591   0.32  +0.4   4,003,466   +    Pf1N1B4_3591   0.34  +0.3   4,003,466   +    Pf1N1B4_3591   0.34  +0.6   4,003,467   -    Pf1N1B4_3591   0.34  -0.6   4,003,467   -    Pf1N1B4_3591   0.34  +0.9   4,003,481   +    Pf1N1B4_3591   0.36  +0.5   4,003,481   +    Pf1N1B4_3591   0.36  +0.7   4,003,481   +    Pf1N1B4_3591   0.36  +1.0   4,003,481   +    Pf1N1B4_3591   0.36  +0.8   4,003,481   +    Pf1N1B4_3591   0.36  +0.3   4,003,482   -    Pf1N1B4_3591   0.36  +0.6   4,003,482   -    Pf1N1B4_3591   0.36  +0.6   4,003,482   -    Pf1N1B4_3591   0.36  +0.3   4,003,482   -    Pf1N1B4_3591   0.36  -0.1   4,003,547   +    Pf1N1B4_3591   0.44  +0.8   4,003,918   +    Pf1N1B4_3591   0.86  +0.7   4,003,918   +    Pf1N1B4_3591   0.86  -0.1   4,004,033   +        +0.3   4,004,033   +        +0.7   4,004,033   +        +0.9   4,004,033   +        -0.6   4,004,033   +        +0.3   4,004,033   +        +0.1   4,004,034   -        +0.4   4,004,034   -        +0.2   4,004,034   -        +0.6   4,004,034   -        +0.6   4,004,034   -        +0.8   4,004,034   -        +0.5   4,004,034   -        +0.7   4,004,034   -        +1.1   4,004,034   -        +0.9   4,004,034   -        +0.3   4,004,034   -        +1.3   4,004,050   +        +0.6   4,004,050   +        -0.7   4,004,050   +        +1.4   4,004,051   -        +0.4   4,004,051   -        +0.7   4,004,083   +        +0.9   4,004,097   -        -0.2   4,004,163   -        -1.1   4,004,163   -        -1.1   4,004,417   +    Pf1N1B4_3592   0.22  +1.1   4,004,417   +    Pf1N1B4_3592   0.22  +0.4   4,004,418   -    Pf1N1B4_3592   0.22  +1.5   4,004,418   -    Pf1N1B4_3592   0.22  +0.2   4,004,418   -    Pf1N1B4_3592   0.22  -1.1   4,004,418   -        -0.1   4,004,423   +    Pf1N1B4_3592   0.23  +0.6   4,004,423   +    Pf1N1B4_3592   0.23  +0.4   4,004,423   +    Pf1N1B4_3592   0.23  +0.7   4,004,423   +    Pf1N1B4_3592   0.23  +1.2   4,004,423   +    Pf1N1B4_3592   0.23  +0.1   4,004,423   +    Pf1N1B4_3592   0.23  +0.7   4,004,423   +    Pf1N1B4_3592   0.23  -0.2   4,004,423   +    Pf1N1B4_3592   0.23  -0.6   4,004,423   +        +0.4   4,004,424   -    Pf1N1B4_3592   0.23  -0.2   4,004,424   -    Pf1N1B4_3592   0.23  -0.3   4,004,424   -    Pf1N1B4_3592   0.23  +0.9   4,004,424   -    Pf1N1B4_3592   0.23  +0.6   4,004,424   -    Pf1N1B4_3592   0.23  -0.2   4,004,424   -    Pf1N1B4_3592   0.23  -0.3   4,004,424   -        +0.7   4,004,424   -    Pf1N1B4_3592   0.23  +1.8   4,004,424   -    Pf1N1B4_3592   0.23  +0.1   4,004,424   -    Pf1N1B4_3592   0.23  +1.1   4,004,424   -    Pf1N1B4_3592   0.23  +1.1   4,004,424   -    Pf1N1B4_3592   0.23  +1.1   4,004,424   -    Pf1N1B4_3592   0.23  -0.2   4,004,424   -    Pf1N1B4_3592   0.23  +0.4   4,004,424   -    Pf1N1B4_3592   0.23  +0.2   4,004,424   -    Pf1N1B4_3592   0.23  +0.7   4,004,424   -    Pf1N1B4_3592   0.23  -0.2   4,004,424   -    Pf1N1B4_3592   0.23  -0.9   4,004,424   -    Pf1N1B4_3592   0.23  +0.9   4,004,424   -    Pf1N1B4_3592   0.23  -0.1   4,004,427   +    Pf1N1B4_3592   0.23  +0.7   4,004,427   +        -0.4   4,004,428   -        +0.7   4,004,428   -    Pf1N1B4_3592   0.23  +0.4   4,004,512   +    Pf1N1B4_3592   0.30  +0.0   4,004,513   -        -0.5   4,004,513   -    Pf1N1B4_3592   0.30  -1.4   4,004,513   -    Pf1N1B4_3592   0.30  -1.1   4,004,513   -    Pf1N1B4_3592   0.30  -0.6   4,004,513   -    Pf1N1B4_3592   0.30  -0.5   4,004,526   -    Pf1N1B4_3592   0.31  -0.1   4,004,526   -    Pf1N1B4_3592   0.31  -0.4   4,004,584   +    Pf1N1B4_3592   0.35  -0.1   4,004,584   +    Pf1N1B4_3592   0.35  -0.4   4,004,585   -    Pf1N1B4_3592   0.35  +0.2   4,004,669   +    Pf1N1B4_3592   0.42  +0.7   4,004,669   +    Pf1N1B4_3592   0.42  -0.2   4,004,669   +    Pf1N1B4_3592   0.42  +0.7   4,004,670   -        +0.9   4,004,919   +    Pf1N1B4_3592   0.61  +0.2   4,004,919   +    Pf1N1B4_3592   0.61  -1.1   4,004,919   +    Pf1N1B4_3592   0.61  +0.4   4,004,958   +    Pf1N1B4_3592   0.64  +0.2   4,004,958   +    Pf1N1B4_3592   0.64  -0.4   4,004,958   +    Pf1N1B4_3592   0.64  +0.8   4,004,958   +    Pf1N1B4_3592   0.64  -0.5   4,004,971   +    Pf1N1B4_3592   0.65  +0.6   4,004,971   +    Pf1N1B4_3592   0.65  -0.1   4,004,971   +    Pf1N1B4_3592   0.65  +1.4   4,004,971   +    Pf1N1B4_3592   0.65  +0.7   4,004,972   -    Pf1N1B4_3592   0.65  -0.9   4,004,972   -    Pf1N1B4_3592   0.65  +0.0   4,004,972   -    Pf1N1B4_3592   0.65  -0.8   4,004,972   -        +0.4   4,004,972   -    Pf1N1B4_3592   0.65  +0.0   4,004,972   -    Pf1N1B4_3592   0.65  +0.3   4,004,972   -    Pf1N1B4_3592   0.65  +0.9   4,004,972   -    Pf1N1B4_3592   0.65  +0.4   4,004,974   -    Pf1N1B4_3592   0.65  -0.0   4,005,022   +    Pf1N1B4_3592   0.69  -0.4   4,005,022   +    Pf1N1B4_3592   0.69  +1.3   4,005,022   +    Pf1N1B4_3592   0.69  +0.4   4,005,022   +    Pf1N1B4_3592   0.69  -0.0   4,005,023   -    Pf1N1B4_3592   0.69  +0.4 
 
Or see this region's nucleotide sequence