Experiment: L-Serine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3103 and Pf1N1B4_3104 are separated by 155 nucleotides Pf1N1B4_3104 and Pf1N1B4_3105 are separated by 171 nucleotides Pf1N1B4_3105 and Pf1N1B4_3106 are separated by 56 nucleotides
Pf1N1B4_3103 - 2-Keto-3-deoxy-D-manno-octulosonate-8-phosphate synthase (EC 2.5.1.55), at 3,490,456 to 3,491,301
_3103
Pf1N1B4_3104 - Enolase (EC 4.2.1.11), at 3,491,457 to 3,492,746
_3104
Pf1N1B4_3105 - Cell division protein DivIC (FtsB), stabilizes FtsL against RasP cleavage, at 3,492,918 to 3,493,196
_3105
Pf1N1B4_3106 - 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase (EC 2.7.7.60), at 3,493,253 to 3,493,900
_3106
Position (kb)
3491
3492
3493 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3491.322 kb on + strand at 3491.322 kb on + strand at 3492.213 kb on + strand, within Pf1N1B4_3104 at 3492.228 kb on + strand, within Pf1N1B4_3104 at 3492.228 kb on + strand, within Pf1N1B4_3104 at 3492.669 kb on + strand at 3492.669 kb on + strand at 3492.669 kb on + strand at 3492.669 kb on + strand at 3492.717 kb on + strand at 3492.717 kb on + strand at 3492.717 kb on + strand at 3492.717 kb on + strand at 3492.717 kb on + strand at 3492.717 kb on + strand at 3492.717 kb on + strand at 3492.751 kb on + strand at 3492.824 kb on + strand at 3492.824 kb on + strand at 3492.824 kb on + strand at 3493.198 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Serine (N) remove 3,491,322 + +0.2 3,491,322 + -0.1 3,492,213 + Pf1N1B4_3104 0.59 -1.4 3,492,228 + Pf1N1B4_3104 0.60 -0.6 3,492,228 + Pf1N1B4_3104 0.60 -1.8 3,492,669 + -3.0 3,492,669 + -3.2 3,492,669 + +0.4 3,492,669 + -3.1 3,492,717 + +0.5 3,492,717 + +1.6 3,492,717 + -0.1 3,492,717 + -1.0 3,492,717 + -1.0 3,492,717 + -0.3 3,492,717 + +2.0 3,492,751 + +0.8 3,492,824 + +0.4 3,492,824 + -0.3 3,492,824 + -0.5 3,493,198 + -1.1
Or see this region's nucleotide sequence