Experiment: L-Serine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_252 and Pf1N1B4_253 overlap by 4 nucleotides Pf1N1B4_253 and Pf1N1B4_254 are separated by 49 nucleotides
Pf1N1B4_252 - Hybrid sensory histidine kinase in two-component regulatory system with EvgA, at 308,358 to 311,261
_252
Pf1N1B4_253 - Hybrid sensory histidine kinase in two-component regulatory system with EvgA, at 311,258 to 312,001
_253
Pf1N1B4_254 - Adenylate cyclase (EC 4.6.1.1), at 312,051 to 314,996
_254
Position (kb) 311
312
313 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 310.365 kb on + strand at 310.365 kb on + strand, within Pf1N1B4_252 at 310.365 kb on + strand, within Pf1N1B4_252 at 310.365 kb on + strand, within Pf1N1B4_252 at 310.366 kb on - strand, within Pf1N1B4_252 at 310.366 kb on - strand, within Pf1N1B4_252 at 310.366 kb on - strand, within Pf1N1B4_252 at 310.465 kb on + strand, within Pf1N1B4_252 at 310.465 kb on + strand, within Pf1N1B4_252 at 310.466 kb on - strand, within Pf1N1B4_252 at 310.466 kb on - strand, within Pf1N1B4_252 at 310.505 kb on + strand, within Pf1N1B4_252 at 310.506 kb on - strand, within Pf1N1B4_252 at 310.506 kb on - strand, within Pf1N1B4_252 at 310.592 kb on + strand, within Pf1N1B4_252 at 310.593 kb on - strand, within Pf1N1B4_252 at 310.635 kb on + strand, within Pf1N1B4_252 at 310.683 kb on - strand, within Pf1N1B4_252 at 310.978 kb on + strand at 311.055 kb on - strand at 311.435 kb on + strand, within Pf1N1B4_253 at 311.876 kb on - strand, within Pf1N1B4_253 at 311.876 kb on - strand at 311.999 kb on + strand at 312.000 kb on - strand at 312.000 kb on - strand at 312.003 kb on + strand at 312.003 kb on + strand at 312.003 kb on + strand at 312.003 kb on + strand at 312.004 kb on - strand at 312.004 kb on - strand at 312.004 kb on - strand at 312.004 kb on - strand at 312.004 kb on - strand at 312.004 kb on - strand at 312.004 kb on - strand at 312.004 kb on - strand at 312.004 kb on - strand at 312.004 kb on - strand at 312.004 kb on - strand at 312.102 kb on - strand at 312.153 kb on - strand at 312.163 kb on - strand at 312.179 kb on + strand at 312.180 kb on - strand at 312.252 kb on + strand at 312.252 kb on + strand at 312.252 kb on + strand at 312.253 kb on - strand at 312.388 kb on - strand, within Pf1N1B4_254 at 312.480 kb on + strand, within Pf1N1B4_254 at 312.481 kb on - strand, within Pf1N1B4_254 at 312.481 kb on - strand at 312.591 kb on + strand, within Pf1N1B4_254 at 312.591 kb on + strand, within Pf1N1B4_254 at 312.591 kb on + strand at 312.591 kb on + strand, within Pf1N1B4_254 at 312.591 kb on + strand, within Pf1N1B4_254 at 312.592 kb on - strand, within Pf1N1B4_254 at 312.592 kb on - strand, within Pf1N1B4_254 at 312.592 kb on - strand, within Pf1N1B4_254 at 312.661 kb on - strand, within Pf1N1B4_254
Per-strain Table
Position Strand Gene LocusTag Fraction L-Serine (N) remove 310,365 + -0.7 310,365 + Pf1N1B4_252 0.69 -0.1 310,365 + Pf1N1B4_252 0.69 +0.6 310,365 + Pf1N1B4_252 0.69 +0.3 310,366 - Pf1N1B4_252 0.69 -0.1 310,366 - Pf1N1B4_252 0.69 +0.4 310,366 - Pf1N1B4_252 0.69 -0.1 310,465 + Pf1N1B4_252 0.73 +0.0 310,465 + Pf1N1B4_252 0.73 +0.1 310,466 - Pf1N1B4_252 0.73 +0.1 310,466 - Pf1N1B4_252 0.73 +0.3 310,505 + Pf1N1B4_252 0.74 -0.8 310,506 - Pf1N1B4_252 0.74 +0.4 310,506 - Pf1N1B4_252 0.74 -0.3 310,592 + Pf1N1B4_252 0.77 +0.3 310,593 - Pf1N1B4_252 0.77 +0.0 310,635 + Pf1N1B4_252 0.78 -1.3 310,683 - Pf1N1B4_252 0.80 -3.1 310,978 + -0.0 311,055 - -0.5 311,435 + Pf1N1B4_253 0.24 +0.4 311,876 - Pf1N1B4_253 0.83 -0.2 311,876 - -0.4 311,999 + +0.4 312,000 - -0.5 312,000 - +0.3 312,003 + -0.4 312,003 + -1.1 312,003 + +0.7 312,003 + +1.3 312,004 - -0.6 312,004 - -0.5 312,004 - -0.3 312,004 - +0.9 312,004 - +0.6 312,004 - +0.1 312,004 - +1.9 312,004 - -1.5 312,004 - +0.2 312,004 - +0.1 312,004 - +0.4 312,102 - +0.1 312,153 - +0.5 312,163 - +0.4 312,179 + +0.5 312,180 - -0.1 312,252 + -0.3 312,252 + +0.4 312,252 + -1.8 312,253 - -0.1 312,388 - Pf1N1B4_254 0.11 -0.4 312,480 + Pf1N1B4_254 0.15 -0.8 312,481 - Pf1N1B4_254 0.15 +0.4 312,481 - +0.9 312,591 + Pf1N1B4_254 0.18 +0.4 312,591 + Pf1N1B4_254 0.18 -1.3 312,591 + +0.4 312,591 + Pf1N1B4_254 0.18 -0.1 312,591 + Pf1N1B4_254 0.18 -0.2 312,592 - Pf1N1B4_254 0.18 +1.9 312,592 - Pf1N1B4_254 0.18 +0.5 312,592 - Pf1N1B4_254 0.18 +0.4 312,661 - Pf1N1B4_254 0.21 -0.5
Or see this region's nucleotide sequence