Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_252

Experiment: L-Serine (N)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_250 and Pf1N1B4_251 are separated by 256 nucleotidesPf1N1B4_251 and Pf1N1B4_252 are separated by 8 nucleotidesPf1N1B4_252 and Pf1N1B4_253 overlap by 4 nucleotidesPf1N1B4_253 and Pf1N1B4_254 are separated by 49 nucleotides Pf1N1B4_250 - response regulator, at 307,074 to 307,466 _250 Pf1N1B4_251 - DNA-binding response regulator, LuxR family, at 307,723 to 308,349 _251 Pf1N1B4_252 - Hybrid sensory histidine kinase in two-component regulatory system with EvgA, at 308,358 to 311,261 _252 Pf1N1B4_253 - Hybrid sensory histidine kinase in two-component regulatory system with EvgA, at 311,258 to 312,001 _253 Pf1N1B4_254 - Adenylate cyclase (EC 4.6.1.1), at 312,051 to 314,996 _254 Position (kb) 308 309 310 311 312Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 307.555 kb on - strandat 307.593 kb on + strandat 307.597 kb on + strandat 307.644 kb on + strandat 307.738 kb on - strandat 307.799 kb on - strand, within Pf1N1B4_251at 308.021 kb on - strand, within Pf1N1B4_251at 308.024 kb on + strand, within Pf1N1B4_251at 308.024 kb on + strandat 308.025 kb on - strandat 308.025 kb on - strand, within Pf1N1B4_251at 308.075 kb on + strand, within Pf1N1B4_251at 308.076 kb on - strandat 308.089 kb on + strand, within Pf1N1B4_251at 308.266 kb on + strand, within Pf1N1B4_251at 308.386 kb on + strandat 308.451 kb on + strandat 308.452 kb on - strandat 308.549 kb on + strandat 308.565 kb on + strandat 308.601 kb on + strandat 308.601 kb on + strandat 308.601 kb on + strandat 308.785 kb on - strand, within Pf1N1B4_252at 308.874 kb on + strand, within Pf1N1B4_252at 308.874 kb on + strand, within Pf1N1B4_252at 308.875 kb on - strand, within Pf1N1B4_252at 308.875 kb on - strand, within Pf1N1B4_252at 308.875 kb on - strand, within Pf1N1B4_252at 308.875 kb on - strand, within Pf1N1B4_252at 308.875 kb on - strand, within Pf1N1B4_252at 308.875 kb on - strand, within Pf1N1B4_252at 308.875 kb on - strand, within Pf1N1B4_252at 308.880 kb on + strand, within Pf1N1B4_252at 308.880 kb on + strand, within Pf1N1B4_252at 308.880 kb on + strand, within Pf1N1B4_252at 308.881 kb on - strand, within Pf1N1B4_252at 308.881 kb on - strand, within Pf1N1B4_252at 308.881 kb on - strand, within Pf1N1B4_252at 308.934 kb on + strand, within Pf1N1B4_252at 308.935 kb on - strand, within Pf1N1B4_252at 308.944 kb on - strand, within Pf1N1B4_252at 309.015 kb on + strand, within Pf1N1B4_252at 309.015 kb on + strand, within Pf1N1B4_252at 309.015 kb on + strand, within Pf1N1B4_252at 309.016 kb on - strand, within Pf1N1B4_252at 309.016 kb on - strand, within Pf1N1B4_252at 309.016 kb on - strand, within Pf1N1B4_252at 309.016 kb on - strand, within Pf1N1B4_252at 309.016 kb on - strand, within Pf1N1B4_252at 309.019 kb on + strand, within Pf1N1B4_252at 309.019 kb on + strand, within Pf1N1B4_252at 309.019 kb on + strand, within Pf1N1B4_252at 309.020 kb on - strand, within Pf1N1B4_252at 309.020 kb on - strand, within Pf1N1B4_252at 309.020 kb on - strand, within Pf1N1B4_252at 309.020 kb on - strand, within Pf1N1B4_252at 309.404 kb on + strand, within Pf1N1B4_252at 309.631 kb on - strand, within Pf1N1B4_252at 309.631 kb on - strandat 309.751 kb on - strand, within Pf1N1B4_252at 309.751 kb on - strand, within Pf1N1B4_252at 309.751 kb on - strand, within Pf1N1B4_252at 309.954 kb on - strand, within Pf1N1B4_252at 309.967 kb on - strand, within Pf1N1B4_252at 309.967 kb on - strand, within Pf1N1B4_252at 309.967 kb on - strand, within Pf1N1B4_252at 309.982 kb on - strand, within Pf1N1B4_252at 309.982 kb on - strand, within Pf1N1B4_252at 309.994 kb on + strandat 309.994 kb on + strand, within Pf1N1B4_252at 309.995 kb on - strand, within Pf1N1B4_252at 309.995 kb on - strand, within Pf1N1B4_252at 310.038 kb on + strand, within Pf1N1B4_252at 310.038 kb on + strand, within Pf1N1B4_252at 310.039 kb on - strand, within Pf1N1B4_252at 310.039 kb on - strand, within Pf1N1B4_252at 310.039 kb on - strand, within Pf1N1B4_252at 310.039 kb on - strand, within Pf1N1B4_252at 310.040 kb on + strand, within Pf1N1B4_252at 310.040 kb on + strand, within Pf1N1B4_252at 310.040 kb on + strand, within Pf1N1B4_252at 310.041 kb on - strand, within Pf1N1B4_252at 310.041 kb on - strand, within Pf1N1B4_252at 310.041 kb on - strand, within Pf1N1B4_252at 310.041 kb on - strand, within Pf1N1B4_252at 310.041 kb on - strand, within Pf1N1B4_252at 310.140 kb on + strand, within Pf1N1B4_252at 310.140 kb on + strand, within Pf1N1B4_252at 310.140 kb on + strand, within Pf1N1B4_252at 310.141 kb on - strand, within Pf1N1B4_252at 310.141 kb on - strand, within Pf1N1B4_252at 310.141 kb on - strand, within Pf1N1B4_252at 310.141 kb on - strand, within Pf1N1B4_252at 310.141 kb on - strand, within Pf1N1B4_252at 310.141 kb on - strand, within Pf1N1B4_252at 310.141 kb on - strand, within Pf1N1B4_252at 310.185 kb on + strand, within Pf1N1B4_252at 310.186 kb on - strand, within Pf1N1B4_252at 310.186 kb on - strand, within Pf1N1B4_252at 310.365 kb on + strandat 310.365 kb on + strand, within Pf1N1B4_252at 310.365 kb on + strand, within Pf1N1B4_252at 310.365 kb on + strand, within Pf1N1B4_252at 310.366 kb on - strand, within Pf1N1B4_252at 310.366 kb on - strand, within Pf1N1B4_252at 310.366 kb on - strand, within Pf1N1B4_252at 310.465 kb on + strand, within Pf1N1B4_252at 310.465 kb on + strand, within Pf1N1B4_252at 310.466 kb on - strand, within Pf1N1B4_252at 310.466 kb on - strand, within Pf1N1B4_252at 310.505 kb on + strand, within Pf1N1B4_252at 310.506 kb on - strand, within Pf1N1B4_252at 310.506 kb on - strand, within Pf1N1B4_252at 310.592 kb on + strand, within Pf1N1B4_252at 310.593 kb on - strand, within Pf1N1B4_252at 310.635 kb on + strand, within Pf1N1B4_252at 310.683 kb on - strand, within Pf1N1B4_252at 310.978 kb on + strandat 311.055 kb on - strandat 311.435 kb on + strand, within Pf1N1B4_253at 311.876 kb on - strand, within Pf1N1B4_253at 311.876 kb on - strandat 311.999 kb on + strandat 312.000 kb on - strandat 312.000 kb on - strandat 312.003 kb on + strandat 312.003 kb on + strandat 312.003 kb on + strandat 312.003 kb on + strandat 312.004 kb on - strandat 312.004 kb on - strandat 312.004 kb on - strandat 312.004 kb on - strandat 312.004 kb on - strandat 312.004 kb on - strandat 312.004 kb on - strandat 312.004 kb on - strandat 312.004 kb on - strandat 312.004 kb on - strandat 312.004 kb on - strandat 312.102 kb on - strandat 312.153 kb on - strandat 312.163 kb on - strandat 312.179 kb on + strandat 312.180 kb on - strandat 312.252 kb on + strandat 312.252 kb on + strandat 312.252 kb on + strandat 312.253 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Serine (N)
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307,555 - +0.2
307,593 + -0.9
307,597 + +0.4
307,644 + -0.6
307,738 - +0.4
307,799 - Pf1N1B4_251 0.12 +0.9
308,021 - Pf1N1B4_251 0.48 +0.2
308,024 + Pf1N1B4_251 0.48 -0.4
308,024 + +1.1
308,025 - -0.9
308,025 - Pf1N1B4_251 0.48 -0.4
308,075 + Pf1N1B4_251 0.56 -1.7
308,076 - +0.3
308,089 + Pf1N1B4_251 0.58 -0.2
308,266 + Pf1N1B4_251 0.87 -0.2
308,386 + +0.4
308,451 + -0.1
308,452 - +0.1
308,549 + +1.2
308,565 + +0.1
308,601 + +1.5
308,601 + +0.0
308,601 + +0.1
308,785 - Pf1N1B4_252 0.15 -0.7
308,874 + Pf1N1B4_252 0.18 -0.5
308,874 + Pf1N1B4_252 0.18 -0.2
308,875 - Pf1N1B4_252 0.18 -0.8
308,875 - Pf1N1B4_252 0.18 +1.2
308,875 - Pf1N1B4_252 0.18 -2.0
308,875 - Pf1N1B4_252 0.18 -0.2
308,875 - Pf1N1B4_252 0.18 +0.7
308,875 - Pf1N1B4_252 0.18 +0.2
308,875 - Pf1N1B4_252 0.18 -0.3
308,880 + Pf1N1B4_252 0.18 -0.4
308,880 + Pf1N1B4_252 0.18 -1.1
308,880 + Pf1N1B4_252 0.18 -0.1
308,881 - Pf1N1B4_252 0.18 -0.3
308,881 - Pf1N1B4_252 0.18 -0.5
308,881 - Pf1N1B4_252 0.18 +0.5
308,934 + Pf1N1B4_252 0.20 -0.8
308,935 - Pf1N1B4_252 0.20 -1.5
308,944 - Pf1N1B4_252 0.20 +0.6
309,015 + Pf1N1B4_252 0.23 +0.0
309,015 + Pf1N1B4_252 0.23 +1.2
309,015 + Pf1N1B4_252 0.23 +1.1
309,016 - Pf1N1B4_252 0.23 -2.5
309,016 - Pf1N1B4_252 0.23 +0.7
309,016 - Pf1N1B4_252 0.23 -1.2
309,016 - Pf1N1B4_252 0.23 +0.0
309,016 - Pf1N1B4_252 0.23 +0.2
309,019 + Pf1N1B4_252 0.23 +0.3
309,019 + Pf1N1B4_252 0.23 -0.6
309,019 + Pf1N1B4_252 0.23 -0.3
309,020 - Pf1N1B4_252 0.23 +0.3
309,020 - Pf1N1B4_252 0.23 -1.0
309,020 - Pf1N1B4_252 0.23 +0.7
309,020 - Pf1N1B4_252 0.23 -1.1
309,404 + Pf1N1B4_252 0.36 -0.5
309,631 - Pf1N1B4_252 0.44 -0.7
309,631 - +0.4
309,751 - Pf1N1B4_252 0.48 -0.1
309,751 - Pf1N1B4_252 0.48 +0.1
309,751 - Pf1N1B4_252 0.48 -0.7
309,954 - Pf1N1B4_252 0.55 -0.9
309,967 - Pf1N1B4_252 0.55 -1.3
309,967 - Pf1N1B4_252 0.55 -1.0
309,967 - Pf1N1B4_252 0.55 +0.1
309,982 - Pf1N1B4_252 0.56 -0.4
309,982 - Pf1N1B4_252 0.56 +0.7
309,994 + +0.9
309,994 + Pf1N1B4_252 0.56 -0.2
309,995 - Pf1N1B4_252 0.56 +1.2
309,995 - Pf1N1B4_252 0.56 -0.7
310,038 + Pf1N1B4_252 0.58 -1.1
310,038 + Pf1N1B4_252 0.58 +0.0
310,039 - Pf1N1B4_252 0.58 -0.2
310,039 - Pf1N1B4_252 0.58 +1.1
310,039 - Pf1N1B4_252 0.58 -0.3
310,039 - Pf1N1B4_252 0.58 +0.9
310,040 + Pf1N1B4_252 0.58 +0.9
310,040 + Pf1N1B4_252 0.58 +1.3
310,040 + Pf1N1B4_252 0.58 -0.4
310,041 - Pf1N1B4_252 0.58 +1.2
310,041 - Pf1N1B4_252 0.58 +0.5
310,041 - Pf1N1B4_252 0.58 -0.4
310,041 - Pf1N1B4_252 0.58 +0.6
310,041 - Pf1N1B4_252 0.58 +0.1
310,140 + Pf1N1B4_252 0.61 -2.5
310,140 + Pf1N1B4_252 0.61 -0.1
310,140 + Pf1N1B4_252 0.61 -0.7
310,141 - Pf1N1B4_252 0.61 +0.0
310,141 - Pf1N1B4_252 0.61 +0.3
310,141 - Pf1N1B4_252 0.61 +0.2
310,141 - Pf1N1B4_252 0.61 -0.4
310,141 - Pf1N1B4_252 0.61 +0.7
310,141 - Pf1N1B4_252 0.61 +0.5
310,141 - Pf1N1B4_252 0.61 +0.7
310,185 + Pf1N1B4_252 0.63 -1.0
310,186 - Pf1N1B4_252 0.63 -0.3
310,186 - Pf1N1B4_252 0.63 -0.5
310,365 + -0.7
310,365 + Pf1N1B4_252 0.69 -0.1
310,365 + Pf1N1B4_252 0.69 +0.6
310,365 + Pf1N1B4_252 0.69 +0.3
310,366 - Pf1N1B4_252 0.69 -0.1
310,366 - Pf1N1B4_252 0.69 +0.4
310,366 - Pf1N1B4_252 0.69 -0.1
310,465 + Pf1N1B4_252 0.73 +0.0
310,465 + Pf1N1B4_252 0.73 +0.1
310,466 - Pf1N1B4_252 0.73 +0.1
310,466 - Pf1N1B4_252 0.73 +0.3
310,505 + Pf1N1B4_252 0.74 -0.8
310,506 - Pf1N1B4_252 0.74 +0.4
310,506 - Pf1N1B4_252 0.74 -0.3
310,592 + Pf1N1B4_252 0.77 +0.3
310,593 - Pf1N1B4_252 0.77 +0.0
310,635 + Pf1N1B4_252 0.78 -1.3
310,683 - Pf1N1B4_252 0.80 -3.1
310,978 + -0.0
311,055 - -0.5
311,435 + Pf1N1B4_253 0.24 +0.4
311,876 - Pf1N1B4_253 0.83 -0.2
311,876 - -0.4
311,999 + +0.4
312,000 - -0.5
312,000 - +0.3
312,003 + -0.4
312,003 + -1.1
312,003 + +0.7
312,003 + +1.3
312,004 - -0.6
312,004 - -0.5
312,004 - -0.3
312,004 - +0.9
312,004 - +0.6
312,004 - +0.1
312,004 - +1.9
312,004 - -1.5
312,004 - +0.2
312,004 - +0.1
312,004 - +0.4
312,102 - +0.1
312,153 - +0.5
312,163 - +0.4
312,179 + +0.5
312,180 - -0.1
312,252 + -0.3
312,252 + +0.4
312,252 + -1.8
312,253 - -0.1

Or see this region's nucleotide sequence