Experiment: L-Serine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2306 and Pf1N1B4_2307 are separated by 623 nucleotides Pf1N1B4_2307 and Pf1N1B4_2308 are separated by 331 nucleotides
Pf1N1B4_2306 - FAD-dependent pyridine nucleotide-disulphide oxidoreductase, at 2,626,434 to 2,627,327
_2306
Pf1N1B4_2307 - LSU m5C1962 methyltransferase RlmI, at 2,627,951 to 2,629,147
_2307
Pf1N1B4_2308 - Predicted signal transduction protein, at 2,629,479 to 2,630,345
_2308
Position (kb)
2627
2628
2629
2630 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2626.984 kb on + strand, within Pf1N1B4_2306 at 2626.984 kb on + strand at 2626.985 kb on - strand, within Pf1N1B4_2306 at 2626.999 kb on + strand, within Pf1N1B4_2306 at 2626.999 kb on + strand, within Pf1N1B4_2306 at 2626.999 kb on + strand, within Pf1N1B4_2306 at 2626.999 kb on + strand, within Pf1N1B4_2306 at 2626.999 kb on + strand, within Pf1N1B4_2306 at 2626.999 kb on + strand, within Pf1N1B4_2306 at 2626.999 kb on + strand, within Pf1N1B4_2306 at 2627.000 kb on - strand, within Pf1N1B4_2306 at 2627.000 kb on - strand, within Pf1N1B4_2306 at 2627.000 kb on - strand, within Pf1N1B4_2306 at 2627.000 kb on - strand, within Pf1N1B4_2306 at 2627.022 kb on + strand, within Pf1N1B4_2306 at 2627.179 kb on - strand, within Pf1N1B4_2306 at 2627.201 kb on - strand, within Pf1N1B4_2306 at 2627.201 kb on - strand, within Pf1N1B4_2306 at 2627.272 kb on - strand at 2627.374 kb on + strand at 2627.393 kb on + strand at 2627.393 kb on + strand at 2627.400 kb on + strand at 2627.453 kb on - strand at 2627.464 kb on - strand at 2627.488 kb on - strand at 2627.488 kb on - strand at 2627.497 kb on + strand at 2627.498 kb on - strand at 2627.505 kb on + strand at 2627.505 kb on + strand at 2627.505 kb on + strand at 2627.536 kb on + strand at 2627.536 kb on + strand at 2627.536 kb on + strand at 2627.536 kb on + strand at 2627.536 kb on + strand at 2627.536 kb on + strand at 2627.536 kb on + strand at 2627.536 kb on + strand at 2627.536 kb on + strand at 2627.536 kb on + strand at 2627.536 kb on + strand at 2627.536 kb on + strand at 2627.536 kb on + strand at 2627.536 kb on + strand at 2627.536 kb on + strand at 2627.536 kb on + strand at 2627.536 kb on + strand at 2627.536 kb on + strand at 2627.536 kb on + strand at 2627.536 kb on + strand at 2627.536 kb on + strand at 2627.536 kb on + strand at 2627.536 kb on + strand at 2627.536 kb on + strand at 2627.536 kb on + strand at 2627.536 kb on + strand at 2627.536 kb on + strand at 2627.536 kb on + strand at 2627.536 kb on + strand at 2627.536 kb on + strand at 2627.536 kb on + strand at 2627.536 kb on + strand at 2627.536 kb on + strand at 2627.536 kb on + strand at 2627.536 kb on + strand at 2627.536 kb on + strand at 2627.537 kb on - strand at 2627.537 kb on - strand at 2627.537 kb on - strand at 2627.537 kb on - strand at 2627.537 kb on - strand at 2627.537 kb on - strand at 2627.537 kb on - strand at 2627.537 kb on - strand at 2627.537 kb on - strand at 2627.537 kb on - strand at 2627.537 kb on - strand at 2627.537 kb on - strand at 2627.537 kb on - strand at 2627.537 kb on - strand at 2627.537 kb on - strand at 2627.537 kb on - strand at 2627.537 kb on - strand at 2627.537 kb on - strand at 2627.537 kb on - strand at 2627.537 kb on - strand at 2627.537 kb on - strand at 2627.537 kb on - strand at 2627.537 kb on - strand at 2627.537 kb on - strand at 2627.537 kb on - strand at 2627.537 kb on - strand at 2627.537 kb on - strand at 2627.537 kb on - strand at 2627.537 kb on - strand at 2627.537 kb on - strand at 2627.537 kb on - strand at 2627.537 kb on - strand at 2627.537 kb on - strand at 2627.537 kb on - strand at 2627.537 kb on - strand at 2627.537 kb on - strand at 2627.537 kb on - strand at 2627.537 kb on - strand at 2627.537 kb on - strand at 2627.540 kb on + strand at 2627.541 kb on - strand at 2627.542 kb on + strand at 2627.582 kb on + strand at 2627.582 kb on + strand at 2627.583 kb on - strand at 2627.586 kb on + strand at 2627.644 kb on + strand at 2627.645 kb on - strand at 2627.645 kb on - strand at 2627.714 kb on - strand at 2627.745 kb on + strand at 2627.745 kb on + strand at 2627.745 kb on + strand at 2627.746 kb on - strand at 2627.746 kb on - strand at 2627.807 kb on + strand at 2627.810 kb on - strand at 2627.867 kb on + strand at 2627.868 kb on - strand at 2627.948 kb on + strand at 2627.949 kb on - strand at 2627.949 kb on - strand at 2627.949 kb on - strand at 2627.992 kb on - strand at 2627.992 kb on - strand at 2628.459 kb on + strand, within Pf1N1B4_2307 at 2628.459 kb on + strand, within Pf1N1B4_2307 at 2628.459 kb on + strand, within Pf1N1B4_2307 at 2628.460 kb on - strand, within Pf1N1B4_2307 at 2628.465 kb on + strand, within Pf1N1B4_2307 at 2628.465 kb on + strand, within Pf1N1B4_2307 at 2628.466 kb on - strand, within Pf1N1B4_2307 at 2628.466 kb on - strand, within Pf1N1B4_2307 at 2628.466 kb on - strand at 2628.477 kb on - strand, within Pf1N1B4_2307 at 2628.496 kb on - strand, within Pf1N1B4_2307 at 2628.496 kb on - strand, within Pf1N1B4_2307 at 2628.531 kb on + strand, within Pf1N1B4_2307 at 2628.531 kb on + strand, within Pf1N1B4_2307 at 2628.532 kb on - strand, within Pf1N1B4_2307 at 2628.623 kb on + strand, within Pf1N1B4_2307 at 2628.637 kb on - strand, within Pf1N1B4_2307 at 2628.841 kb on - strand, within Pf1N1B4_2307 at 2629.058 kb on + strand at 2629.059 kb on - strand at 2629.077 kb on + strand at 2629.077 kb on + strand at 2629.077 kb on + strand at 2629.078 kb on - strand at 2629.176 kb on + strand at 2629.176 kb on + strand at 2629.176 kb on + strand at 2629.176 kb on + strand at 2629.176 kb on + strand at 2629.176 kb on + strand at 2629.176 kb on + strand at 2629.177 kb on - strand at 2629.178 kb on + strand at 2629.178 kb on + strand at 2629.178 kb on + strand at 2629.178 kb on + strand at 2629.178 kb on + strand at 2629.261 kb on - strand at 2629.447 kb on + strand at 2629.448 kb on - strand at 2629.448 kb on - strand at 2629.448 kb on - strand at 2629.474 kb on + strand at 2629.474 kb on + strand at 2629.474 kb on + strand at 2629.474 kb on + strand at 2629.474 kb on + strand at 2629.474 kb on + strand at 2629.474 kb on + strand at 2629.475 kb on - strand at 2629.475 kb on - strand at 2629.475 kb on - strand at 2629.475 kb on - strand at 2629.475 kb on - strand at 2629.475 kb on - strand at 2629.475 kb on - strand at 2629.475 kb on - strand at 2629.478 kb on + strand at 2629.478 kb on + strand at 2629.478 kb on + strand at 2629.478 kb on + strand at 2629.478 kb on + strand at 2629.478 kb on + strand at 2629.478 kb on + strand at 2629.478 kb on + strand at 2629.478 kb on + strand at 2629.478 kb on + strand at 2629.478 kb on + strand at 2629.478 kb on + strand at 2629.478 kb on + strand at 2629.479 kb on - strand at 2629.479 kb on - strand at 2629.479 kb on - strand at 2629.479 kb on - strand at 2629.479 kb on - strand at 2629.479 kb on - strand at 2629.479 kb on - strand at 2629.479 kb on - strand at 2629.479 kb on - strand at 2629.479 kb on - strand at 2629.479 kb on - strand at 2629.479 kb on - strand at 2629.479 kb on - strand at 2629.479 kb on - strand at 2629.479 kb on - strand at 2629.479 kb on - strand at 2629.479 kb on - strand at 2629.479 kb on - strand at 2629.479 kb on - strand at 2629.479 kb on - strand at 2629.479 kb on - strand at 2629.578 kb on + strand, within Pf1N1B4_2308 at 2629.578 kb on + strand, within Pf1N1B4_2308 at 2629.578 kb on + strand, within Pf1N1B4_2308 at 2629.578 kb on + strand, within Pf1N1B4_2308 at 2629.578 kb on + strand, within Pf1N1B4_2308 at 2629.578 kb on + strand, within Pf1N1B4_2308 at 2629.578 kb on + strand, within Pf1N1B4_2308 at 2629.578 kb on + strand, within Pf1N1B4_2308 at 2629.578 kb on + strand, within Pf1N1B4_2308 at 2629.578 kb on + strand, within Pf1N1B4_2308 at 2629.578 kb on + strand, within Pf1N1B4_2308 at 2629.579 kb on - strand, within Pf1N1B4_2308 at 2629.579 kb on - strand, within Pf1N1B4_2308 at 2629.579 kb on - strand, within Pf1N1B4_2308 at 2629.579 kb on - strand, within Pf1N1B4_2308 at 2629.579 kb on - strand, within Pf1N1B4_2308 at 2629.579 kb on - strand, within Pf1N1B4_2308 at 2629.579 kb on - strand, within Pf1N1B4_2308 at 2629.579 kb on - strand, within Pf1N1B4_2308 at 2629.579 kb on - strand, within Pf1N1B4_2308 at 2629.675 kb on - strand, within Pf1N1B4_2308 at 2629.675 kb on - strand, within Pf1N1B4_2308 at 2629.685 kb on + strand, within Pf1N1B4_2308 at 2629.685 kb on + strand, within Pf1N1B4_2308 at 2629.686 kb on - strand, within Pf1N1B4_2308 at 2629.797 kb on - strand, within Pf1N1B4_2308 at 2629.797 kb on - strand, within Pf1N1B4_2308 at 2629.890 kb on + strand, within Pf1N1B4_2308 at 2629.891 kb on - strand, within Pf1N1B4_2308 at 2629.891 kb on - strand, within Pf1N1B4_2308 at 2629.891 kb on - strand, within Pf1N1B4_2308 at 2629.950 kb on + strand, within Pf1N1B4_2308 at 2629.950 kb on + strand, within Pf1N1B4_2308 at 2629.950 kb on + strand, within Pf1N1B4_2308 at 2629.950 kb on + strand, within Pf1N1B4_2308 at 2629.951 kb on - strand, within Pf1N1B4_2308 at 2629.951 kb on - strand, within Pf1N1B4_2308 at 2629.951 kb on - strand, within Pf1N1B4_2308 at 2629.974 kb on + strand, within Pf1N1B4_2308 at 2629.975 kb on - strand, within Pf1N1B4_2308 at 2629.975 kb on - strand, within Pf1N1B4_2308 at 2630.026 kb on - strand, within Pf1N1B4_2308 at 2630.055 kb on + strand, within Pf1N1B4_2308 at 2630.055 kb on + strand, within Pf1N1B4_2308 at 2630.055 kb on + strand, within Pf1N1B4_2308 at 2630.055 kb on + strand, within Pf1N1B4_2308 at 2630.055 kb on + strand, within Pf1N1B4_2308 at 2630.055 kb on + strand, within Pf1N1B4_2308 at 2630.056 kb on - strand, within Pf1N1B4_2308 at 2630.056 kb on - strand, within Pf1N1B4_2308 at 2630.056 kb on - strand, within Pf1N1B4_2308 at 2630.056 kb on - strand, within Pf1N1B4_2308 at 2630.056 kb on - strand, within Pf1N1B4_2308 at 2630.056 kb on - strand, within Pf1N1B4_2308 at 2630.056 kb on - strand, within Pf1N1B4_2308
Per-strain Table
Position Strand Gene LocusTag Fraction L-Serine (N) remove 2,626,984 + Pf1N1B4_2306 0.62 -0.5 2,626,984 + +1.3 2,626,985 - Pf1N1B4_2306 0.62 +1.3 2,626,999 + Pf1N1B4_2306 0.63 +0.6 2,626,999 + Pf1N1B4_2306 0.63 +1.5 2,626,999 + Pf1N1B4_2306 0.63 -0.3 2,626,999 + Pf1N1B4_2306 0.63 -0.2 2,626,999 + Pf1N1B4_2306 0.63 +0.9 2,626,999 + Pf1N1B4_2306 0.63 -0.4 2,626,999 + Pf1N1B4_2306 0.63 -1.0 2,627,000 - Pf1N1B4_2306 0.63 -0.2 2,627,000 - Pf1N1B4_2306 0.63 -0.0 2,627,000 - Pf1N1B4_2306 0.63 -0.3 2,627,000 - Pf1N1B4_2306 0.63 -1.4 2,627,022 + Pf1N1B4_2306 0.66 +0.8 2,627,179 - Pf1N1B4_2306 0.83 -0.6 2,627,201 - Pf1N1B4_2306 0.86 -0.1 2,627,201 - Pf1N1B4_2306 0.86 -0.0 2,627,272 - +0.4 2,627,374 + -0.2 2,627,393 + -0.6 2,627,393 + +0.7 2,627,400 + -0.5 2,627,453 - -0.1 2,627,464 - +1.1 2,627,488 - +1.9 2,627,488 - -0.4 2,627,497 + -0.5 2,627,498 - +0.9 2,627,505 + +1.3 2,627,505 + +0.1 2,627,505 + -1.4 2,627,536 + +1.3 2,627,536 + +0.1 2,627,536 + +0.2 2,627,536 + -0.6 2,627,536 + +1.3 2,627,536 + -0.3 2,627,536 + +1.3 2,627,536 + -0.7 2,627,536 + -0.7 2,627,536 + +0.6 2,627,536 + +0.6 2,627,536 + +0.6 2,627,536 + +0.4 2,627,536 + -0.6 2,627,536 + -1.0 2,627,536 + +0.1 2,627,536 + +1.1 2,627,536 + -0.2 2,627,536 + -0.0 2,627,536 + +0.6 2,627,536 + -1.6 2,627,536 + -2.3 2,627,536 + +0.5 2,627,536 + +0.2 2,627,536 + -0.5 2,627,536 + -1.5 2,627,536 + -2.0 2,627,536 + -0.4 2,627,536 + +0.2 2,627,536 + +0.8 2,627,536 + -0.3 2,627,536 + +0.7 2,627,536 + -1.0 2,627,536 + +0.2 2,627,536 + -0.0 2,627,536 + -0.3 2,627,537 - +0.3 2,627,537 - +0.8 2,627,537 - -2.4 2,627,537 - +0.3 2,627,537 - +0.3 2,627,537 - -1.2 2,627,537 - +0.5 2,627,537 - -0.5 2,627,537 - -0.1 2,627,537 - +0.1 2,627,537 - +0.3 2,627,537 - +0.5 2,627,537 - -0.1 2,627,537 - +1.7 2,627,537 - +0.2 2,627,537 - +0.5 2,627,537 - -2.0 2,627,537 - -1.0 2,627,537 - +0.1 2,627,537 - +0.2 2,627,537 - +0.7 2,627,537 - -0.0 2,627,537 - -1.1 2,627,537 - -1.0 2,627,537 - -2.0 2,627,537 - +0.8 2,627,537 - -1.4 2,627,537 - +0.3 2,627,537 - +0.4 2,627,537 - -0.8 2,627,537 - -0.9 2,627,537 - +1.1 2,627,537 - -0.1 2,627,537 - -0.0 2,627,537 - -0.4 2,627,537 - -1.0 2,627,537 - +1.1 2,627,537 - -0.0 2,627,537 - +0.7 2,627,540 + +0.9 2,627,541 - +1.0 2,627,542 + +0.5 2,627,582 + -0.8 2,627,582 + +1.5 2,627,583 - -0.1 2,627,586 + -0.0 2,627,644 + +0.3 2,627,645 - -0.7 2,627,645 - +0.8 2,627,714 - -0.0 2,627,745 + -0.7 2,627,745 + -0.4 2,627,745 + -0.2 2,627,746 - +0.7 2,627,746 - +0.4 2,627,807 + -1.0 2,627,810 - +0.3 2,627,867 + -0.7 2,627,868 - -0.1 2,627,948 + -1.0 2,627,949 - -0.8 2,627,949 - -0.3 2,627,949 - -0.6 2,627,992 - -1.0 2,627,992 - +0.3 2,628,459 + Pf1N1B4_2307 0.42 +1.0 2,628,459 + Pf1N1B4_2307 0.42 +1.1 2,628,459 + Pf1N1B4_2307 0.42 +0.5 2,628,460 - Pf1N1B4_2307 0.43 +0.9 2,628,465 + Pf1N1B4_2307 0.43 +0.1 2,628,465 + Pf1N1B4_2307 0.43 -1.5 2,628,466 - Pf1N1B4_2307 0.43 +0.8 2,628,466 - Pf1N1B4_2307 0.43 +0.5 2,628,466 - +0.6 2,628,477 - Pf1N1B4_2307 0.44 +0.7 2,628,496 - Pf1N1B4_2307 0.46 +1.3 2,628,496 - Pf1N1B4_2307 0.46 -0.7 2,628,531 + Pf1N1B4_2307 0.48 +0.3 2,628,531 + Pf1N1B4_2307 0.48 +0.0 2,628,532 - Pf1N1B4_2307 0.49 +1.1 2,628,623 + Pf1N1B4_2307 0.56 +1.0 2,628,637 - Pf1N1B4_2307 0.57 -0.7 2,628,841 - Pf1N1B4_2307 0.74 +0.5 2,629,058 + -0.1 2,629,059 - -0.9 2,629,077 + +1.6 2,629,077 + -0.5 2,629,077 + +0.2 2,629,078 - -0.7 2,629,176 + +1.1 2,629,176 + +1.0 2,629,176 + -0.2 2,629,176 + +0.6 2,629,176 + +1.3 2,629,176 + +0.1 2,629,176 + -0.6 2,629,177 - +0.7 2,629,178 + -1.8 2,629,178 + -1.1 2,629,178 + +0.8 2,629,178 + +0.6 2,629,178 + +0.3 2,629,261 - +0.0 2,629,447 + -0.3 2,629,448 - +0.5 2,629,448 - -1.0 2,629,448 - +1.0 2,629,474 + -0.0 2,629,474 + -0.4 2,629,474 + -0.1 2,629,474 + +0.6 2,629,474 + -0.3 2,629,474 + +2.9 2,629,474 + -0.6 2,629,475 - +0.2 2,629,475 - +0.2 2,629,475 - -0.0 2,629,475 - -0.4 2,629,475 - +0.7 2,629,475 - -0.1 2,629,475 - -1.0 2,629,475 - -1.3 2,629,478 + +1.0 2,629,478 + -0.3 2,629,478 + +0.6 2,629,478 + -0.6 2,629,478 + +2.3 2,629,478 + -0.8 2,629,478 + +1.3 2,629,478 + +1.3 2,629,478 + +0.1 2,629,478 + -0.1 2,629,478 + -0.7 2,629,478 + -1.0 2,629,478 + +0.6 2,629,479 - +0.4 2,629,479 - -0.1 2,629,479 - +0.2 2,629,479 - -3.1 2,629,479 - +0.5 2,629,479 - -0.5 2,629,479 - +0.3 2,629,479 - +0.7 2,629,479 - -2.3 2,629,479 - +1.7 2,629,479 - -1.1 2,629,479 - -0.6 2,629,479 - -0.8 2,629,479 - +0.7 2,629,479 - -0.7 2,629,479 - -0.1 2,629,479 - +1.1 2,629,479 - -0.1 2,629,479 - +0.1 2,629,479 - +1.3 2,629,479 - +0.8 2,629,578 + Pf1N1B4_2308 0.11 -0.5 2,629,578 + Pf1N1B4_2308 0.11 +0.8 2,629,578 + Pf1N1B4_2308 0.11 +0.2 2,629,578 + Pf1N1B4_2308 0.11 -0.6 2,629,578 + Pf1N1B4_2308 0.11 +0.3 2,629,578 + Pf1N1B4_2308 0.11 +0.4 2,629,578 + Pf1N1B4_2308 0.11 +0.4 2,629,578 + Pf1N1B4_2308 0.11 +1.0 2,629,578 + Pf1N1B4_2308 0.11 -0.0 2,629,578 + Pf1N1B4_2308 0.11 -0.7 2,629,578 + Pf1N1B4_2308 0.11 -0.9 2,629,579 - Pf1N1B4_2308 0.12 -0.3 2,629,579 - Pf1N1B4_2308 0.12 -0.1 2,629,579 - Pf1N1B4_2308 0.12 -0.3 2,629,579 - Pf1N1B4_2308 0.12 -0.8 2,629,579 - Pf1N1B4_2308 0.12 +0.7 2,629,579 - Pf1N1B4_2308 0.12 -0.1 2,629,579 - Pf1N1B4_2308 0.12 -0.5 2,629,579 - Pf1N1B4_2308 0.12 +0.3 2,629,579 - Pf1N1B4_2308 0.12 -0.2 2,629,675 - Pf1N1B4_2308 0.23 -0.3 2,629,675 - Pf1N1B4_2308 0.23 -0.6 2,629,685 + Pf1N1B4_2308 0.24 -0.7 2,629,685 + Pf1N1B4_2308 0.24 +0.7 2,629,686 - Pf1N1B4_2308 0.24 -1.8 2,629,797 - Pf1N1B4_2308 0.37 -0.0 2,629,797 - Pf1N1B4_2308 0.37 -0.0 2,629,890 + Pf1N1B4_2308 0.47 +0.3 2,629,891 - Pf1N1B4_2308 0.48 +1.3 2,629,891 - Pf1N1B4_2308 0.48 +1.3 2,629,891 - Pf1N1B4_2308 0.48 -1.7 2,629,950 + Pf1N1B4_2308 0.54 -1.0 2,629,950 + Pf1N1B4_2308 0.54 +1.2 2,629,950 + Pf1N1B4_2308 0.54 -1.1 2,629,950 + Pf1N1B4_2308 0.54 +0.3 2,629,951 - Pf1N1B4_2308 0.54 +0.0 2,629,951 - Pf1N1B4_2308 0.54 -0.4 2,629,951 - Pf1N1B4_2308 0.54 -0.2 2,629,974 + Pf1N1B4_2308 0.57 -1.1 2,629,975 - Pf1N1B4_2308 0.57 -0.6 2,629,975 - Pf1N1B4_2308 0.57 -0.1 2,630,026 - Pf1N1B4_2308 0.63 -0.7 2,630,055 + Pf1N1B4_2308 0.66 -0.3 2,630,055 + Pf1N1B4_2308 0.66 +0.7 2,630,055 + Pf1N1B4_2308 0.66 +0.3 2,630,055 + Pf1N1B4_2308 0.66 +1.5 2,630,055 + Pf1N1B4_2308 0.66 -0.4 2,630,055 + Pf1N1B4_2308 0.66 +0.7 2,630,056 - Pf1N1B4_2308 0.67 +1.3 2,630,056 - Pf1N1B4_2308 0.67 -0.0 2,630,056 - Pf1N1B4_2308 0.67 +1.1 2,630,056 - Pf1N1B4_2308 0.67 -0.9 2,630,056 - Pf1N1B4_2308 0.67 -1.0 2,630,056 - Pf1N1B4_2308 0.67 -0.4 2,630,056 - Pf1N1B4_2308 0.67 -0.7
Or see this region's nucleotide sequence