Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_2235

Experiment: L-Serine (N)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_2234 and Pf1N1B4_2235 are separated by 50 nucleotidesPf1N1B4_2235 and Pf1N1B4_2236 are separated by 269 nucleotides Pf1N1B4_2234 - Aminomethyltransferase (glycine cleavage system T protein) (EC 2.1.2.10), at 2,547,299 to 2,548,132 _2234 Pf1N1B4_2235 - Glycine cleavage system H protein, at 2,548,183 to 2,548,569 _2235 Pf1N1B4_2236 - Glycine dehydrogenase [decarboxylating] (glycine cleavage system P protein) (EC 1.4.4.2), at 2,548,839 to 2,551,712 _2236 Position (kb) 2548 2549Strain fitness (log2 ratio) -2 -1 0 1 2at 2547.196 kb on + strandat 2547.252 kb on - strandat 2547.255 kb on + strandat 2547.256 kb on - strandat 2547.303 kb on - strandat 2547.303 kb on - strandat 2547.354 kb on - strandat 2547.563 kb on + strand, within Pf1N1B4_2234at 2547.564 kb on - strand, within Pf1N1B4_2234at 2547.691 kb on + strand, within Pf1N1B4_2234at 2547.692 kb on - strand, within Pf1N1B4_2234at 2547.879 kb on + strand, within Pf1N1B4_2234at 2547.879 kb on + strand, within Pf1N1B4_2234at 2547.982 kb on + strand, within Pf1N1B4_2234at 2547.983 kb on - strand, within Pf1N1B4_2234at 2547.983 kb on - strand, within Pf1N1B4_2234at 2547.983 kb on - strand, within Pf1N1B4_2234at 2547.983 kb on - strand, within Pf1N1B4_2234at 2547.994 kb on + strand, within Pf1N1B4_2234at 2547.994 kb on + strand, within Pf1N1B4_2234at 2547.994 kb on + strand, within Pf1N1B4_2234at 2548.001 kb on - strand, within Pf1N1B4_2234at 2548.027 kb on - strand, within Pf1N1B4_2234at 2548.131 kb on - strandat 2548.238 kb on + strand, within Pf1N1B4_2235at 2548.238 kb on + strand, within Pf1N1B4_2235at 2548.239 kb on - strand, within Pf1N1B4_2235at 2548.239 kb on - strand, within Pf1N1B4_2235at 2548.266 kb on + strand, within Pf1N1B4_2235at 2548.266 kb on + strand, within Pf1N1B4_2235at 2548.266 kb on + strand, within Pf1N1B4_2235at 2548.301 kb on + strand, within Pf1N1B4_2235at 2548.301 kb on + strand, within Pf1N1B4_2235at 2548.301 kb on + strand, within Pf1N1B4_2235at 2548.301 kb on + strand, within Pf1N1B4_2235at 2548.301 kb on + strand, within Pf1N1B4_2235at 2548.301 kb on + strand, within Pf1N1B4_2235at 2548.301 kb on + strand, within Pf1N1B4_2235at 2548.301 kb on + strand, within Pf1N1B4_2235at 2548.301 kb on + strand, within Pf1N1B4_2235at 2548.301 kb on + strand, within Pf1N1B4_2235at 2548.301 kb on + strand, within Pf1N1B4_2235at 2548.301 kb on + strand, within Pf1N1B4_2235at 2548.301 kb on + strand, within Pf1N1B4_2235at 2548.301 kb on + strand, within Pf1N1B4_2235at 2548.301 kb on + strand, within Pf1N1B4_2235at 2548.301 kb on + strand, within Pf1N1B4_2235at 2548.301 kb on + strand, within Pf1N1B4_2235at 2548.301 kb on + strand, within Pf1N1B4_2235at 2548.301 kb on + strand, within Pf1N1B4_2235at 2548.301 kb on + strand, within Pf1N1B4_2235at 2548.301 kb on + strand, within Pf1N1B4_2235at 2548.301 kb on + strand, within Pf1N1B4_2235at 2548.301 kb on + strand, within Pf1N1B4_2235at 2548.301 kb on + strand, within Pf1N1B4_2235at 2548.301 kb on + strand, within Pf1N1B4_2235at 2548.301 kb on + strand, within Pf1N1B4_2235at 2548.301 kb on + strand, within Pf1N1B4_2235at 2548.302 kb on - strand, within Pf1N1B4_2235at 2548.302 kb on - strand, within Pf1N1B4_2235at 2548.302 kb on - strand, within Pf1N1B4_2235at 2548.302 kb on - strand, within Pf1N1B4_2235at 2548.302 kb on - strand, within Pf1N1B4_2235at 2548.302 kb on - strand, within Pf1N1B4_2235at 2548.302 kb on - strand, within Pf1N1B4_2235at 2548.393 kb on + strand, within Pf1N1B4_2235at 2548.393 kb on + strand, within Pf1N1B4_2235at 2548.393 kb on + strand, within Pf1N1B4_2235at 2548.393 kb on + strand, within Pf1N1B4_2235at 2548.393 kb on + strand, within Pf1N1B4_2235at 2548.393 kb on + strand, within Pf1N1B4_2235at 2548.393 kb on + strand, within Pf1N1B4_2235at 2548.393 kb on + strand, within Pf1N1B4_2235at 2548.393 kb on + strand, within Pf1N1B4_2235at 2548.393 kb on + strand, within Pf1N1B4_2235at 2548.393 kb on + strand, within Pf1N1B4_2235at 2548.393 kb on + strand, within Pf1N1B4_2235at 2548.394 kb on - strand, within Pf1N1B4_2235at 2548.394 kb on - strand, within Pf1N1B4_2235at 2548.394 kb on - strand, within Pf1N1B4_2235at 2548.471 kb on + strand, within Pf1N1B4_2235at 2548.471 kb on + strand, within Pf1N1B4_2235at 2548.471 kb on + strand, within Pf1N1B4_2235at 2548.471 kb on + strand, within Pf1N1B4_2235at 2548.471 kb on + strand, within Pf1N1B4_2235at 2548.546 kb on + strandat 2548.546 kb on + strandat 2548.546 kb on + strandat 2548.546 kb on + strandat 2548.546 kb on + strandat 2548.546 kb on + strandat 2548.546 kb on + strandat 2548.546 kb on + strandat 2548.546 kb on + strandat 2548.547 kb on - strandat 2548.547 kb on - strandat 2548.547 kb on - strandat 2548.567 kb on + strandat 2548.568 kb on - strandat 2548.582 kb on - strandat 2548.642 kb on + strandat 2548.642 kb on + strandat 2548.643 kb on - strandat 2548.643 kb on - strandat 2548.689 kb on + strandat 2548.689 kb on + strandat 2548.689 kb on + strandat 2548.690 kb on - strandat 2548.691 kb on + strandat 2548.691 kb on + strandat 2548.692 kb on - strandat 2548.692 kb on - strandat 2548.739 kb on + strandat 2548.739 kb on + strandat 2548.739 kb on + strandat 2548.739 kb on + strandat 2548.739 kb on + strandat 2548.782 kb on + strandat 2548.783 kb on - strandat 2548.880 kb on + strandat 2548.880 kb on + strandat 2549.170 kb on - strand, within Pf1N1B4_2236at 2549.170 kb on - strand, within Pf1N1B4_2236at 2549.170 kb on - strand, within Pf1N1B4_2236at 2549.178 kb on + strand, within Pf1N1B4_2236at 2549.178 kb on + strand, within Pf1N1B4_2236at 2549.179 kb on - strand, within Pf1N1B4_2236at 2549.179 kb on - strand, within Pf1N1B4_2236at 2549.352 kb on - strand, within Pf1N1B4_2236at 2549.352 kb on - strand, within Pf1N1B4_2236at 2549.352 kb on - strand, within Pf1N1B4_2236at 2549.352 kb on - strand, within Pf1N1B4_2236at 2549.494 kb on - strand, within Pf1N1B4_2236

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Serine (N)
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2,547,196 + +0.6
2,547,252 - +0.1
2,547,255 + -0.1
2,547,256 - -1.5
2,547,303 - +0.2
2,547,303 - -0.4
2,547,354 - +1.1
2,547,563 + Pf1N1B4_2234 0.32 -1.1
2,547,564 - Pf1N1B4_2234 0.32 -0.2
2,547,691 + Pf1N1B4_2234 0.47 -2.0
2,547,692 - Pf1N1B4_2234 0.47 -0.2
2,547,879 + Pf1N1B4_2234 0.70 -0.5
2,547,879 + Pf1N1B4_2234 0.70 -1.5
2,547,982 + Pf1N1B4_2234 0.82 +0.0
2,547,983 - Pf1N1B4_2234 0.82 +0.3
2,547,983 - Pf1N1B4_2234 0.82 +0.3
2,547,983 - Pf1N1B4_2234 0.82 +0.1
2,547,983 - Pf1N1B4_2234 0.82 -0.0
2,547,994 + Pf1N1B4_2234 0.83 -0.5
2,547,994 + Pf1N1B4_2234 0.83 +0.6
2,547,994 + Pf1N1B4_2234 0.83 -1.1
2,548,001 - Pf1N1B4_2234 0.84 +0.9
2,548,027 - Pf1N1B4_2234 0.87 -0.9
2,548,131 - +0.4
2,548,238 + Pf1N1B4_2235 0.14 +1.0
2,548,238 + Pf1N1B4_2235 0.14 -0.4
2,548,239 - Pf1N1B4_2235 0.14 -0.4
2,548,239 - Pf1N1B4_2235 0.14 +0.3
2,548,266 + Pf1N1B4_2235 0.21 +0.9
2,548,266 + Pf1N1B4_2235 0.21 +0.7
2,548,266 + Pf1N1B4_2235 0.21 +0.8
2,548,301 + Pf1N1B4_2235 0.30 +2.0
2,548,301 + Pf1N1B4_2235 0.30 +0.6
2,548,301 + Pf1N1B4_2235 0.30 -1.2
2,548,301 + Pf1N1B4_2235 0.30 +0.0
2,548,301 + Pf1N1B4_2235 0.30 +0.8
2,548,301 + Pf1N1B4_2235 0.30 +0.6
2,548,301 + Pf1N1B4_2235 0.30 -0.1
2,548,301 + Pf1N1B4_2235 0.30 +0.3
2,548,301 + Pf1N1B4_2235 0.30 -0.6
2,548,301 + Pf1N1B4_2235 0.30 +0.3
2,548,301 + Pf1N1B4_2235 0.30 +0.3
2,548,301 + Pf1N1B4_2235 0.30 -0.3
2,548,301 + Pf1N1B4_2235 0.30 +1.3
2,548,301 + Pf1N1B4_2235 0.30 +1.3
2,548,301 + Pf1N1B4_2235 0.30 +0.8
2,548,301 + Pf1N1B4_2235 0.30 -0.4
2,548,301 + Pf1N1B4_2235 0.30 +0.2
2,548,301 + Pf1N1B4_2235 0.30 -0.0
2,548,301 + Pf1N1B4_2235 0.30 +1.1
2,548,301 + Pf1N1B4_2235 0.30 +0.5
2,548,301 + Pf1N1B4_2235 0.30 -0.2
2,548,301 + Pf1N1B4_2235 0.30 +1.2
2,548,301 + Pf1N1B4_2235 0.30 +0.5
2,548,301 + Pf1N1B4_2235 0.30 +0.9
2,548,301 + Pf1N1B4_2235 0.30 +1.7
2,548,301 + Pf1N1B4_2235 0.30 +0.3
2,548,301 + Pf1N1B4_2235 0.30 +1.2
2,548,302 - Pf1N1B4_2235 0.31 +0.5
2,548,302 - Pf1N1B4_2235 0.31 +1.7
2,548,302 - Pf1N1B4_2235 0.31 -0.1
2,548,302 - Pf1N1B4_2235 0.31 +0.5
2,548,302 - Pf1N1B4_2235 0.31 -0.2
2,548,302 - Pf1N1B4_2235 0.31 +0.3
2,548,302 - Pf1N1B4_2235 0.31 +1.9
2,548,393 + Pf1N1B4_2235 0.54 +0.9
2,548,393 + Pf1N1B4_2235 0.54 +0.1
2,548,393 + Pf1N1B4_2235 0.54 +0.3
2,548,393 + Pf1N1B4_2235 0.54 +0.8
2,548,393 + Pf1N1B4_2235 0.54 -0.0
2,548,393 + Pf1N1B4_2235 0.54 +0.0
2,548,393 + Pf1N1B4_2235 0.54 +0.8
2,548,393 + Pf1N1B4_2235 0.54 +0.6
2,548,393 + Pf1N1B4_2235 0.54 +1.2
2,548,393 + Pf1N1B4_2235 0.54 +1.9
2,548,393 + Pf1N1B4_2235 0.54 +0.3
2,548,393 + Pf1N1B4_2235 0.54 +0.3
2,548,394 - Pf1N1B4_2235 0.55 -0.0
2,548,394 - Pf1N1B4_2235 0.55 -0.8
2,548,394 - Pf1N1B4_2235 0.55 +0.5
2,548,471 + Pf1N1B4_2235 0.74 +0.4
2,548,471 + Pf1N1B4_2235 0.74 +0.6
2,548,471 + Pf1N1B4_2235 0.74 +0.6
2,548,471 + Pf1N1B4_2235 0.74 +0.3
2,548,471 + Pf1N1B4_2235 0.74 +1.0
2,548,546 + +1.2
2,548,546 + -0.7
2,548,546 + +0.4
2,548,546 + +0.3
2,548,546 + -0.5
2,548,546 + +0.2
2,548,546 + +1.9
2,548,546 + +0.7
2,548,546 + +1.1
2,548,547 - +0.3
2,548,547 - +0.6
2,548,547 - +0.2
2,548,567 + +0.8
2,548,568 - -0.3
2,548,582 - +0.5
2,548,642 + -1.1
2,548,642 + +1.5
2,548,643 - -0.9
2,548,643 - +0.3
2,548,689 + +0.5
2,548,689 + +0.9
2,548,689 + +0.6
2,548,690 - +0.2
2,548,691 + +2.1
2,548,691 + -1.2
2,548,692 - -0.2
2,548,692 - +1.2
2,548,739 + +0.2
2,548,739 + -0.8
2,548,739 + -0.8
2,548,739 + +0.8
2,548,739 + -0.6
2,548,782 + -0.1
2,548,783 - +0.9
2,548,880 + -0.5
2,548,880 + +1.3
2,549,170 - Pf1N1B4_2236 0.12 +1.3
2,549,170 - Pf1N1B4_2236 0.12 -0.1
2,549,170 - Pf1N1B4_2236 0.12 -0.0
2,549,178 + Pf1N1B4_2236 0.12 +1.3
2,549,178 + Pf1N1B4_2236 0.12 +0.0
2,549,179 - Pf1N1B4_2236 0.12 +0.9
2,549,179 - Pf1N1B4_2236 0.12 -0.5
2,549,352 - Pf1N1B4_2236 0.18 +1.0
2,549,352 - Pf1N1B4_2236 0.18 +0.3
2,549,352 - Pf1N1B4_2236 0.18 -0.8
2,549,352 - Pf1N1B4_2236 0.18 -0.0
2,549,494 - Pf1N1B4_2236 0.23 -0.6

Or see this region's nucleotide sequence