Experiment: L-Serine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1831 and Pf1N1B4_1832 are separated by 112 nucleotides Pf1N1B4_1832 and Pf1N1B4_1833 overlap by 4 nucleotides Pf1N1B4_1833 and Pf1N1B4_1834 are separated by 154 nucleotides Pf1N1B4_1834 and Pf1N1B4_1835 are separated by 130 nucleotides
Pf1N1B4_1831 - Tryptophan synthase beta chain (EC 4.2.1.20), at 2,107,311 to 2,108,540
_1831
Pf1N1B4_1832 - Glycine cleavage system transcriptional activator, at 2,108,653 to 2,109,546
_1832
Pf1N1B4_1833 - FIG00953718: hypothetical protein, at 2,109,543 to 2,109,869
_1833
Pf1N1B4_1834 - Dodecin (COG3360) Flavin-binding, at 2,110,024 to 2,110,239
_1834
Pf1N1B4_1835 - hypothetical protein, at 2,110,370 to 2,110,594
_1835
Position (kb)
2108
2109
2110 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3 at 2107.961 kb on - strand, within Pf1N1B4_1831 at 2107.961 kb on - strand, within Pf1N1B4_1831 at 2107.961 kb on - strand, within Pf1N1B4_1831 at 2107.961 kb on - strand, within Pf1N1B4_1831 at 2108.019 kb on - strand, within Pf1N1B4_1831 at 2108.019 kb on - strand, within Pf1N1B4_1831 at 2108.120 kb on - strand, within Pf1N1B4_1831 at 2108.120 kb on - strand, within Pf1N1B4_1831 at 2108.120 kb on - strand, within Pf1N1B4_1831 at 2108.120 kb on - strand, within Pf1N1B4_1831 at 2108.120 kb on - strand, within Pf1N1B4_1831 at 2108.120 kb on - strand, within Pf1N1B4_1831 at 2108.120 kb on - strand, within Pf1N1B4_1831 at 2108.120 kb on - strand, within Pf1N1B4_1831 at 2108.120 kb on - strand, within Pf1N1B4_1831 at 2108.120 kb on - strand, within Pf1N1B4_1831 at 2108.120 kb on - strand, within Pf1N1B4_1831 at 2108.120 kb on - strand, within Pf1N1B4_1831 at 2108.120 kb on - strand, within Pf1N1B4_1831 at 2108.120 kb on - strand, within Pf1N1B4_1831 at 2108.120 kb on - strand, within Pf1N1B4_1831 at 2108.120 kb on - strand, within Pf1N1B4_1831 at 2108.120 kb on - strand, within Pf1N1B4_1831 at 2108.120 kb on - strand, within Pf1N1B4_1831 at 2108.120 kb on - strand, within Pf1N1B4_1831 at 2108.120 kb on - strand, within Pf1N1B4_1831 at 2108.120 kb on - strand, within Pf1N1B4_1831 at 2108.120 kb on - strand, within Pf1N1B4_1831 at 2108.120 kb on - strand, within Pf1N1B4_1831 at 2108.120 kb on - strand, within Pf1N1B4_1831 at 2108.120 kb on - strand, within Pf1N1B4_1831 at 2108.120 kb on - strand, within Pf1N1B4_1831 at 2108.120 kb on - strand, within Pf1N1B4_1831 at 2108.120 kb on - strand, within Pf1N1B4_1831 at 2108.120 kb on - strand, within Pf1N1B4_1831 at 2108.120 kb on - strand, within Pf1N1B4_1831 at 2108.120 kb on - strand, within Pf1N1B4_1831 at 2108.120 kb on - strand, within Pf1N1B4_1831 at 2108.297 kb on - strand, within Pf1N1B4_1831 at 2108.297 kb on - strand, within Pf1N1B4_1831 at 2108.297 kb on - strand, within Pf1N1B4_1831 at 2108.297 kb on - strand, within Pf1N1B4_1831 at 2108.297 kb on - strand, within Pf1N1B4_1831 at 2108.342 kb on - strand, within Pf1N1B4_1831 at 2108.342 kb on - strand, within Pf1N1B4_1831 at 2108.342 kb on - strand, within Pf1N1B4_1831 at 2108.342 kb on - strand, within Pf1N1B4_1831 at 2108.342 kb on - strand, within Pf1N1B4_1831 at 2108.342 kb on - strand at 2108.342 kb on - strand, within Pf1N1B4_1831 at 2108.342 kb on - strand, within Pf1N1B4_1831 at 2108.342 kb on - strand, within Pf1N1B4_1831 at 2108.342 kb on - strand, within Pf1N1B4_1831 at 2108.366 kb on - strand, within Pf1N1B4_1831 at 2108.366 kb on - strand, within Pf1N1B4_1831 at 2108.366 kb on - strand, within Pf1N1B4_1831 at 2108.366 kb on - strand, within Pf1N1B4_1831 at 2108.366 kb on - strand, within Pf1N1B4_1831 at 2108.366 kb on - strand, within Pf1N1B4_1831 at 2108.366 kb on - strand, within Pf1N1B4_1831 at 2108.366 kb on - strand, within Pf1N1B4_1831 at 2108.366 kb on - strand, within Pf1N1B4_1831 at 2108.381 kb on - strand, within Pf1N1B4_1831 at 2108.381 kb on - strand, within Pf1N1B4_1831 at 2108.381 kb on - strand, within Pf1N1B4_1831 at 2108.426 kb on - strand at 2108.529 kb on - strand at 2108.529 kb on - strand at 2108.556 kb on + strand at 2108.556 kb on + strand at 2108.556 kb on + strand at 2108.556 kb on + strand at 2108.556 kb on + strand at 2108.557 kb on - strand at 2108.557 kb on - strand at 2108.557 kb on - strand at 2108.585 kb on + strand at 2109.089 kb on + strand, within Pf1N1B4_1832 at 2109.089 kb on + strand, within Pf1N1B4_1832 at 2109.090 kb on - strand, within Pf1N1B4_1832 at 2109.090 kb on - strand, within Pf1N1B4_1832 at 2109.127 kb on + strand, within Pf1N1B4_1832 at 2109.127 kb on + strand, within Pf1N1B4_1832 at 2109.128 kb on - strand, within Pf1N1B4_1832 at 2109.307 kb on + strand, within Pf1N1B4_1832 at 2109.307 kb on + strand, within Pf1N1B4_1832 at 2109.307 kb on + strand, within Pf1N1B4_1832 at 2109.307 kb on + strand, within Pf1N1B4_1832 at 2109.308 kb on - strand, within Pf1N1B4_1832 at 2109.308 kb on - strand, within Pf1N1B4_1832 at 2109.308 kb on - strand, within Pf1N1B4_1832 at 2109.308 kb on - strand, within Pf1N1B4_1832 at 2109.308 kb on - strand, within Pf1N1B4_1832 at 2109.308 kb on - strand, within Pf1N1B4_1832 at 2109.308 kb on - strand, within Pf1N1B4_1832 at 2109.308 kb on - strand, within Pf1N1B4_1832 at 2109.308 kb on - strand, within Pf1N1B4_1832 at 2109.332 kb on - strand, within Pf1N1B4_1832 at 2109.332 kb on - strand at 2109.332 kb on - strand, within Pf1N1B4_1832 at 2109.332 kb on - strand, within Pf1N1B4_1832 at 2109.334 kb on + strand, within Pf1N1B4_1832 at 2109.334 kb on + strand, within Pf1N1B4_1832 at 2109.334 kb on + strand, within Pf1N1B4_1832 at 2109.335 kb on - strand, within Pf1N1B4_1832 at 2109.519 kb on + strand at 2109.544 kb on + strand at 2109.544 kb on + strand at 2109.544 kb on + strand at 2109.544 kb on + strand at 2109.544 kb on + strand at 2109.544 kb on + strand at 2109.544 kb on + strand at 2109.544 kb on + strand at 2109.544 kb on + strand at 2109.544 kb on + strand at 2109.544 kb on + strand at 2109.544 kb on + strand at 2109.545 kb on - strand at 2109.545 kb on - strand at 2109.545 kb on - strand at 2109.545 kb on - strand at 2109.545 kb on - strand at 2109.545 kb on - strand at 2109.548 kb on + strand at 2109.548 kb on + strand at 2109.548 kb on + strand at 2109.568 kb on + strand at 2109.569 kb on - strand at 2109.634 kb on + strand, within Pf1N1B4_1833 at 2109.634 kb on + strand, within Pf1N1B4_1833 at 2109.635 kb on - strand, within Pf1N1B4_1833 at 2109.635 kb on - strand, within Pf1N1B4_1833 at 2109.635 kb on - strand, within Pf1N1B4_1833 at 2109.686 kb on - strand, within Pf1N1B4_1833 at 2109.686 kb on - strand, within Pf1N1B4_1833 at 2109.759 kb on - strand, within Pf1N1B4_1833 at 2109.848 kb on + strand at 2109.848 kb on + strand at 2109.848 kb on + strand at 2109.848 kb on + strand at 2109.849 kb on - strand at 2109.849 kb on - strand at 2109.849 kb on - strand at 2109.852 kb on + strand at 2109.853 kb on - strand at 2109.853 kb on - strand at 2109.937 kb on + strand at 2109.937 kb on + strand at 2109.937 kb on + strand at 2109.938 kb on - strand at 2109.938 kb on - strand at 2109.976 kb on + strand at 2109.977 kb on - strand at 2109.977 kb on - strand at 2109.992 kb on + strand at 2109.992 kb on + strand at 2109.992 kb on + strand at 2109.992 kb on + strand at 2109.993 kb on - strand at 2109.993 kb on - strand at 2109.993 kb on - strand at 2109.993 kb on - strand at 2109.999 kb on + strand at 2109.999 kb on + strand at 2110.000 kb on - strand at 2110.000 kb on - strand at 2110.000 kb on - strand at 2110.012 kb on + strand at 2110.012 kb on + strand at 2110.042 kb on + strand at 2110.042 kb on + strand at 2110.042 kb on + strand at 2110.042 kb on + strand at 2110.042 kb on + strand at 2110.043 kb on - strand at 2110.043 kb on - strand at 2110.043 kb on - strand at 2110.132 kb on + strand, within Pf1N1B4_1834 at 2110.132 kb on + strand, within Pf1N1B4_1834 at 2110.132 kb on + strand, within Pf1N1B4_1834 at 2110.132 kb on + strand, within Pf1N1B4_1834 at 2110.132 kb on + strand, within Pf1N1B4_1834 at 2110.132 kb on + strand, within Pf1N1B4_1834 at 2110.132 kb on + strand, within Pf1N1B4_1834 at 2110.132 kb on + strand, within Pf1N1B4_1834 at 2110.132 kb on + strand, within Pf1N1B4_1834 at 2110.132 kb on + strand, within Pf1N1B4_1834 at 2110.132 kb on + strand, within Pf1N1B4_1834 at 2110.132 kb on + strand, within Pf1N1B4_1834 at 2110.133 kb on - strand, within Pf1N1B4_1834 at 2110.133 kb on - strand, within Pf1N1B4_1834 at 2110.133 kb on - strand, within Pf1N1B4_1834 at 2110.133 kb on - strand, within Pf1N1B4_1834 at 2110.233 kb on + strand at 2110.234 kb on - strand at 2110.234 kb on - strand at 2110.234 kb on - strand at 2110.244 kb on + strand at 2110.245 kb on - strand at 2110.248 kb on + strand at 2110.248 kb on + strand at 2110.248 kb on + strand at 2110.248 kb on + strand at 2110.393 kb on - strand, within Pf1N1B4_1835 at 2110.496 kb on + strand, within Pf1N1B4_1835 at 2110.496 kb on + strand at 2110.497 kb on - strand, within Pf1N1B4_1835 at 2110.497 kb on - strand, within Pf1N1B4_1835
Per-strain Table
Position Strand Gene LocusTag Fraction L-Serine (N) remove 2,107,961 - Pf1N1B4_1831 0.53 +0.4 2,107,961 - Pf1N1B4_1831 0.53 -2.2 2,107,961 - Pf1N1B4_1831 0.53 -3.0 2,107,961 - Pf1N1B4_1831 0.53 +0.8 2,108,019 - Pf1N1B4_1831 0.58 -0.6 2,108,019 - Pf1N1B4_1831 0.58 -4.3 2,108,120 - Pf1N1B4_1831 0.66 -3.8 2,108,120 - Pf1N1B4_1831 0.66 -3.2 2,108,120 - Pf1N1B4_1831 0.66 -2.1 2,108,120 - Pf1N1B4_1831 0.66 -3.7 2,108,120 - Pf1N1B4_1831 0.66 -3.6 2,108,120 - Pf1N1B4_1831 0.66 -3.0 2,108,120 - Pf1N1B4_1831 0.66 -4.0 2,108,120 - Pf1N1B4_1831 0.66 -0.3 2,108,120 - Pf1N1B4_1831 0.66 -1.6 2,108,120 - Pf1N1B4_1831 0.66 -2.0 2,108,120 - Pf1N1B4_1831 0.66 -2.7 2,108,120 - Pf1N1B4_1831 0.66 -2.8 2,108,120 - Pf1N1B4_1831 0.66 -1.8 2,108,120 - Pf1N1B4_1831 0.66 -2.6 2,108,120 - Pf1N1B4_1831 0.66 -1.7 2,108,120 - Pf1N1B4_1831 0.66 -2.2 2,108,120 - Pf1N1B4_1831 0.66 -5.1 2,108,120 - Pf1N1B4_1831 0.66 -2.8 2,108,120 - Pf1N1B4_1831 0.66 -3.8 2,108,120 - Pf1N1B4_1831 0.66 -2.5 2,108,120 - Pf1N1B4_1831 0.66 -2.8 2,108,120 - Pf1N1B4_1831 0.66 -2.5 2,108,120 - Pf1N1B4_1831 0.66 -3.2 2,108,120 - Pf1N1B4_1831 0.66 -3.2 2,108,120 - Pf1N1B4_1831 0.66 -3.2 2,108,120 - Pf1N1B4_1831 0.66 -2.6 2,108,120 - Pf1N1B4_1831 0.66 -3.1 2,108,120 - Pf1N1B4_1831 0.66 -3.3 2,108,120 - Pf1N1B4_1831 0.66 -3.7 2,108,120 - Pf1N1B4_1831 0.66 -2.1 2,108,120 - Pf1N1B4_1831 0.66 -1.6 2,108,120 - Pf1N1B4_1831 0.66 -1.2 2,108,297 - Pf1N1B4_1831 0.80 -2.0 2,108,297 - Pf1N1B4_1831 0.80 -2.0 2,108,297 - Pf1N1B4_1831 0.80 -2.2 2,108,297 - Pf1N1B4_1831 0.80 -3.2 2,108,297 - Pf1N1B4_1831 0.80 -5.0 2,108,342 - Pf1N1B4_1831 0.84 -2.1 2,108,342 - Pf1N1B4_1831 0.84 -4.0 2,108,342 - Pf1N1B4_1831 0.84 -3.2 2,108,342 - Pf1N1B4_1831 0.84 -3.0 2,108,342 - Pf1N1B4_1831 0.84 -0.8 2,108,342 - +1.4 2,108,342 - Pf1N1B4_1831 0.84 -3.2 2,108,342 - Pf1N1B4_1831 0.84 -2.2 2,108,342 - Pf1N1B4_1831 0.84 -2.5 2,108,342 - Pf1N1B4_1831 0.84 -4.2 2,108,366 - Pf1N1B4_1831 0.86 -3.2 2,108,366 - Pf1N1B4_1831 0.86 -3.5 2,108,366 - Pf1N1B4_1831 0.86 -1.0 2,108,366 - Pf1N1B4_1831 0.86 -2.2 2,108,366 - Pf1N1B4_1831 0.86 -2.4 2,108,366 - Pf1N1B4_1831 0.86 -1.0 2,108,366 - Pf1N1B4_1831 0.86 -1.8 2,108,366 - Pf1N1B4_1831 0.86 -1.8 2,108,366 - Pf1N1B4_1831 0.86 -2.0 2,108,381 - Pf1N1B4_1831 0.87 -1.8 2,108,381 - Pf1N1B4_1831 0.87 -2.7 2,108,381 - Pf1N1B4_1831 0.87 -3.1 2,108,426 - -1.9 2,108,529 - -1.4 2,108,529 - -1.2 2,108,556 + -1.2 2,108,556 + -1.0 2,108,556 + -1.2 2,108,556 + -1.6 2,108,556 + -2.4 2,108,557 - -0.4 2,108,557 - -0.5 2,108,557 - +0.0 2,108,585 + -2.0 2,109,089 + Pf1N1B4_1832 0.49 -0.2 2,109,089 + Pf1N1B4_1832 0.49 +0.4 2,109,090 - Pf1N1B4_1832 0.49 -0.2 2,109,090 - Pf1N1B4_1832 0.49 -0.5 2,109,127 + Pf1N1B4_1832 0.53 +1.1 2,109,127 + Pf1N1B4_1832 0.53 -1.0 2,109,128 - Pf1N1B4_1832 0.53 +0.2 2,109,307 + Pf1N1B4_1832 0.73 -0.8 2,109,307 + Pf1N1B4_1832 0.73 -0.4 2,109,307 + Pf1N1B4_1832 0.73 -0.2 2,109,307 + Pf1N1B4_1832 0.73 -0.2 2,109,308 - Pf1N1B4_1832 0.73 -0.8 2,109,308 - Pf1N1B4_1832 0.73 -0.3 2,109,308 - Pf1N1B4_1832 0.73 +1.1 2,109,308 - Pf1N1B4_1832 0.73 -0.5 2,109,308 - Pf1N1B4_1832 0.73 -0.3 2,109,308 - Pf1N1B4_1832 0.73 +0.1 2,109,308 - Pf1N1B4_1832 0.73 -1.6 2,109,308 - Pf1N1B4_1832 0.73 +0.3 2,109,308 - Pf1N1B4_1832 0.73 +0.8 2,109,332 - Pf1N1B4_1832 0.76 +0.2 2,109,332 - +1.9 2,109,332 - Pf1N1B4_1832 0.76 +0.1 2,109,332 - Pf1N1B4_1832 0.76 -0.8 2,109,334 + Pf1N1B4_1832 0.76 +1.6 2,109,334 + Pf1N1B4_1832 0.76 -1.8 2,109,334 + Pf1N1B4_1832 0.76 +0.6 2,109,335 - Pf1N1B4_1832 0.76 -0.6 2,109,519 + -0.4 2,109,544 + +0.0 2,109,544 + +0.7 2,109,544 + +1.5 2,109,544 + -0.4 2,109,544 + -0.4 2,109,544 + +0.7 2,109,544 + +1.9 2,109,544 + -0.0 2,109,544 + +0.1 2,109,544 + -0.2 2,109,544 + -0.2 2,109,544 + +0.6 2,109,545 - -0.2 2,109,545 - -0.3 2,109,545 - -0.3 2,109,545 - +1.6 2,109,545 - -0.4 2,109,545 - -0.6 2,109,548 + -0.6 2,109,548 + -0.1 2,109,548 + -0.4 2,109,568 + -0.6 2,109,569 - -0.4 2,109,634 + Pf1N1B4_1833 0.28 -0.2 2,109,634 + Pf1N1B4_1833 0.28 +0.2 2,109,635 - Pf1N1B4_1833 0.28 +0.0 2,109,635 - Pf1N1B4_1833 0.28 +0.4 2,109,635 - Pf1N1B4_1833 0.28 +1.1 2,109,686 - Pf1N1B4_1833 0.44 +0.2 2,109,686 - Pf1N1B4_1833 0.44 +0.5 2,109,759 - Pf1N1B4_1833 0.66 -0.8 2,109,848 + +1.1 2,109,848 + +0.9 2,109,848 + -0.6 2,109,848 + -0.4 2,109,849 - +0.8 2,109,849 - -0.3 2,109,849 - +0.1 2,109,852 + +1.4 2,109,853 - +0.2 2,109,853 - +0.6 2,109,937 + -1.6 2,109,937 + -0.6 2,109,937 + -0.6 2,109,938 - -0.8 2,109,938 - -0.0 2,109,976 + +0.4 2,109,977 - -0.6 2,109,977 - +0.4 2,109,992 + -0.4 2,109,992 + -0.6 2,109,992 + +1.0 2,109,992 + +0.3 2,109,993 - -1.3 2,109,993 - +0.5 2,109,993 - -1.2 2,109,993 - +0.2 2,109,999 + -1.0 2,109,999 + +0.2 2,110,000 - +0.5 2,110,000 - +1.8 2,110,000 - +1.5 2,110,012 + +3.4 2,110,012 + -0.4 2,110,042 + +0.4 2,110,042 + -0.4 2,110,042 + -0.2 2,110,042 + -0.3 2,110,042 + +0.1 2,110,043 - -0.2 2,110,043 - -0.6 2,110,043 - -0.6 2,110,132 + Pf1N1B4_1834 0.50 -0.9 2,110,132 + Pf1N1B4_1834 0.50 +0.4 2,110,132 + Pf1N1B4_1834 0.50 +0.7 2,110,132 + Pf1N1B4_1834 0.50 +0.1 2,110,132 + Pf1N1B4_1834 0.50 -0.4 2,110,132 + Pf1N1B4_1834 0.50 -1.6 2,110,132 + Pf1N1B4_1834 0.50 +1.2 2,110,132 + Pf1N1B4_1834 0.50 -0.2 2,110,132 + Pf1N1B4_1834 0.50 +0.1 2,110,132 + Pf1N1B4_1834 0.50 -1.3 2,110,132 + Pf1N1B4_1834 0.50 +0.9 2,110,132 + Pf1N1B4_1834 0.50 +0.0 2,110,133 - Pf1N1B4_1834 0.50 -2.3 2,110,133 - Pf1N1B4_1834 0.50 -0.5 2,110,133 - Pf1N1B4_1834 0.50 +0.9 2,110,133 - Pf1N1B4_1834 0.50 -0.0 2,110,233 + +0.4 2,110,234 - -0.6 2,110,234 - -0.7 2,110,234 - -0.1 2,110,244 + -0.1 2,110,245 - -0.5 2,110,248 + -0.6 2,110,248 + -0.1 2,110,248 + -0.2 2,110,248 + +1.1 2,110,393 - Pf1N1B4_1835 0.10 -0.2 2,110,496 + Pf1N1B4_1835 0.56 -1.1 2,110,496 + +0.4 2,110,497 - Pf1N1B4_1835 0.56 -1.3 2,110,497 - Pf1N1B4_1835 0.56 -0.5
Or see this region's nucleotide sequence