Experiment: L-Serine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_113 and Pf1N1B4_114 are separated by 112 nucleotides Pf1N1B4_114 and Pf1N1B4_115 are separated by 169 nucleotides
Pf1N1B4_113 - Cytosine deaminase (EC 3.5.4.1), at 117,512 to 118,756
_113
Pf1N1B4_114 - Diaminopimelate epimerase (EC 5.1.1.7), at 118,869 to 119,858
_114
Pf1N1B4_115 - Membrane protein mosC, at 120,028 to 121,179
_115
Position (kb)
118
119
120 Strain fitness (log2 ratio)
-1
0
1 at 118.059 kb on - strand, within Pf1N1B4_113 at 118.653 kb on - strand at 118.864 kb on - strand at 118.864 kb on - strand at 119.164 kb on + strand, within Pf1N1B4_114 at 119.164 kb on + strand, within Pf1N1B4_114 at 119.165 kb on - strand, within Pf1N1B4_114 at 119.165 kb on - strand, within Pf1N1B4_114 at 119.165 kb on - strand, within Pf1N1B4_114 at 119.693 kb on + strand, within Pf1N1B4_114 at 119.694 kb on - strand, within Pf1N1B4_114 at 119.824 kb on + strand at 119.825 kb on - strand at 119.825 kb on - strand at 119.825 kb on - strand at 119.828 kb on + strand at 119.829 kb on - strand at 119.829 kb on - strand at 119.829 kb on - strand at 119.829 kb on - strand at 119.845 kb on + strand at 119.846 kb on - strand at 119.846 kb on - strand at 119.872 kb on - strand at 119.872 kb on - strand at 119.872 kb on - strand at 120.027 kb on + strand at 120.145 kb on + strand, within Pf1N1B4_115 at 120.330 kb on + strand, within Pf1N1B4_115 at 120.843 kb on + strand, within Pf1N1B4_115
Per-strain Table
Position Strand Gene LocusTag Fraction L-Serine (N) remove 118,059 - Pf1N1B4_113 0.44 -0.2 118,653 - -0.7 118,864 - -0.8 118,864 - -0.9 119,164 + Pf1N1B4_114 0.30 +0.7 119,164 + Pf1N1B4_114 0.30 -0.5 119,165 - Pf1N1B4_114 0.30 +0.2 119,165 - Pf1N1B4_114 0.30 +0.5 119,165 - Pf1N1B4_114 0.30 +1.1 119,693 + Pf1N1B4_114 0.83 -0.2 119,694 - Pf1N1B4_114 0.83 +0.5 119,824 + +0.0 119,825 - -0.9 119,825 - -0.0 119,825 - -1.0 119,828 + -1.4 119,829 - +0.1 119,829 - -0.4 119,829 - -0.8 119,829 - -0.4 119,845 + +0.7 119,846 - +0.4 119,846 - +0.2 119,872 - +0.6 119,872 - -0.0 119,872 - +0.4 120,027 + +0.4 120,145 + Pf1N1B4_115 0.10 -0.4 120,330 + Pf1N1B4_115 0.26 -0.2 120,843 + Pf1N1B4_115 0.71 +0.2
Or see this region's nucleotide sequence