Experiment: L-Serine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1042 and Pf1N1B4_1043 are separated by 96 nucleotides Pf1N1B4_1043 and Pf1N1B4_1044 overlap by 8 nucleotides Pf1N1B4_1044 and Pf1N1B4_1045 are separated by 194 nucleotides Pf1N1B4_1045 and Pf1N1B4_1046 are separated by 181 nucleotides
Pf1N1B4_1042 - Dihydroneopterin triphosphate epimerase, at 1,180,111 to 1,180,482
_1042
Pf1N1B4_1043 - FIG00953380: hypothetical protein, at 1,180,579 to 1,180,875
_1043
Pf1N1B4_1044 - Type III effector HopPmaJ, at 1,180,868 to 1,181,206
_1044
Pf1N1B4_1045 - Gamma-aminobutyrate:alpha-ketoglutarate aminotransferase (EC 2.6.1.19), at 1,181,401 to 1,182,651
_1045
Pf1N1B4_1046 - Thioredoxin reductase (EC 1.8.1.9), at 1,182,833 to 1,183,789
_1046
Position (kb)
1181
1182
1183 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1180.402 kb on + strand, within Pf1N1B4_1042 at 1180.402 kb on + strand, within Pf1N1B4_1042 at 1180.507 kb on + strand at 1180.508 kb on - strand at 1180.508 kb on - strand at 1180.508 kb on - strand at 1180.717 kb on + strand, within Pf1N1B4_1043 at 1180.717 kb on + strand, within Pf1N1B4_1043 at 1180.717 kb on + strand, within Pf1N1B4_1043 at 1180.717 kb on + strand, within Pf1N1B4_1043 at 1180.717 kb on + strand, within Pf1N1B4_1043 at 1180.717 kb on + strand, within Pf1N1B4_1043 at 1180.717 kb on + strand, within Pf1N1B4_1043 at 1180.717 kb on + strand, within Pf1N1B4_1043 at 1180.717 kb on + strand, within Pf1N1B4_1043 at 1180.717 kb on + strand, within Pf1N1B4_1043 at 1180.717 kb on + strand, within Pf1N1B4_1043 at 1180.717 kb on + strand, within Pf1N1B4_1043 at 1180.718 kb on - strand, within Pf1N1B4_1043 at 1180.718 kb on - strand, within Pf1N1B4_1043 at 1180.718 kb on - strand, within Pf1N1B4_1043 at 1180.718 kb on - strand at 1180.718 kb on - strand, within Pf1N1B4_1043 at 1180.718 kb on - strand at 1180.719 kb on + strand, within Pf1N1B4_1043 at 1180.719 kb on + strand, within Pf1N1B4_1043 at 1180.720 kb on - strand, within Pf1N1B4_1043 at 1180.720 kb on - strand, within Pf1N1B4_1043 at 1180.720 kb on - strand, within Pf1N1B4_1043 at 1180.720 kb on - strand, within Pf1N1B4_1043 at 1180.780 kb on + strand, within Pf1N1B4_1043 at 1180.792 kb on + strand, within Pf1N1B4_1043 at 1180.883 kb on - strand at 1180.928 kb on - strand, within Pf1N1B4_1044 at 1180.953 kb on - strand, within Pf1N1B4_1044 at 1181.279 kb on + strand at 1181.285 kb on + strand at 1181.285 kb on + strand at 1181.285 kb on + strand at 1181.285 kb on + strand at 1181.286 kb on - strand at 1181.286 kb on - strand at 1181.286 kb on - strand at 1181.286 kb on - strand at 1181.286 kb on - strand at 1181.308 kb on + strand at 1181.309 kb on - strand at 1181.309 kb on - strand at 1181.309 kb on - strand at 1181.328 kb on + strand at 1181.328 kb on + strand at 1181.329 kb on - strand at 1181.361 kb on + strand at 1181.361 kb on + strand at 1181.361 kb on + strand at 1181.361 kb on + strand at 1181.362 kb on - strand at 1181.362 kb on - strand at 1181.366 kb on - strand at 1181.366 kb on - strand at 1181.500 kb on + strand at 1181.500 kb on + strand at 1181.500 kb on + strand at 1181.500 kb on + strand at 1181.500 kb on + strand at 1181.501 kb on - strand at 1181.630 kb on - strand, within Pf1N1B4_1045 at 1181.630 kb on - strand, within Pf1N1B4_1045 at 1181.694 kb on + strand, within Pf1N1B4_1045 at 1181.695 kb on - strand, within Pf1N1B4_1045 at 1181.836 kb on + strand, within Pf1N1B4_1045 at 1181.836 kb on + strand, within Pf1N1B4_1045 at 1181.837 kb on - strand, within Pf1N1B4_1045 at 1181.983 kb on - strand, within Pf1N1B4_1045 at 1181.983 kb on - strand, within Pf1N1B4_1045 at 1182.265 kb on + strand, within Pf1N1B4_1045 at 1182.266 kb on - strand, within Pf1N1B4_1045 at 1182.266 kb on - strand, within Pf1N1B4_1045 at 1182.266 kb on - strand, within Pf1N1B4_1045 at 1182.266 kb on - strand, within Pf1N1B4_1045 at 1182.266 kb on - strand, within Pf1N1B4_1045 at 1182.266 kb on - strand, within Pf1N1B4_1045 at 1182.409 kb on + strand, within Pf1N1B4_1045 at 1182.410 kb on - strand, within Pf1N1B4_1045 at 1182.410 kb on - strand, within Pf1N1B4_1045 at 1182.654 kb on + strand at 1182.654 kb on + strand at 1182.704 kb on + strand at 1182.704 kb on + strand at 1182.705 kb on - strand at 1182.705 kb on - strand at 1182.717 kb on + strand at 1182.717 kb on + strand at 1182.733 kb on - strand at 1182.767 kb on + strand at 1182.768 kb on - strand at 1182.768 kb on - strand at 1182.786 kb on + strand at 1182.787 kb on - strand at 1182.820 kb on + strand at 1182.820 kb on + strand at 1182.821 kb on - strand at 1182.821 kb on - strand at 1182.821 kb on - strand at 1182.821 kb on - strand at 1182.821 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Serine (N) remove 1,180,402 + Pf1N1B4_1042 0.78 +1.9 1,180,402 + Pf1N1B4_1042 0.78 -0.8 1,180,507 + +0.0 1,180,508 - +0.2 1,180,508 - +0.1 1,180,508 - -0.5 1,180,717 + Pf1N1B4_1043 0.46 -0.2 1,180,717 + Pf1N1B4_1043 0.46 +1.7 1,180,717 + Pf1N1B4_1043 0.46 -0.5 1,180,717 + Pf1N1B4_1043 0.46 -0.4 1,180,717 + Pf1N1B4_1043 0.46 -0.7 1,180,717 + Pf1N1B4_1043 0.46 +0.5 1,180,717 + Pf1N1B4_1043 0.46 +0.4 1,180,717 + Pf1N1B4_1043 0.46 -0.3 1,180,717 + Pf1N1B4_1043 0.46 +0.5 1,180,717 + Pf1N1B4_1043 0.46 -1.0 1,180,717 + Pf1N1B4_1043 0.46 +3.0 1,180,717 + Pf1N1B4_1043 0.46 -1.3 1,180,718 - Pf1N1B4_1043 0.47 +0.7 1,180,718 - Pf1N1B4_1043 0.47 +1.5 1,180,718 - Pf1N1B4_1043 0.47 +1.0 1,180,718 - +0.7 1,180,718 - Pf1N1B4_1043 0.47 -0.7 1,180,718 - +1.6 1,180,719 + Pf1N1B4_1043 0.47 +0.5 1,180,719 + Pf1N1B4_1043 0.47 -0.4 1,180,720 - Pf1N1B4_1043 0.47 +1.3 1,180,720 - Pf1N1B4_1043 0.47 -0.1 1,180,720 - Pf1N1B4_1043 0.47 +0.5 1,180,720 - Pf1N1B4_1043 0.47 +0.3 1,180,780 + Pf1N1B4_1043 0.68 -0.0 1,180,792 + Pf1N1B4_1043 0.72 -0.2 1,180,883 - -0.6 1,180,928 - Pf1N1B4_1044 0.18 -0.3 1,180,953 - Pf1N1B4_1044 0.25 +0.3 1,181,279 + +0.4 1,181,285 + -0.3 1,181,285 + -0.6 1,181,285 + +1.1 1,181,285 + +0.1 1,181,286 - +0.3 1,181,286 - +0.6 1,181,286 - -0.2 1,181,286 - +0.3 1,181,286 - +0.4 1,181,308 + -0.4 1,181,309 - -0.3 1,181,309 - -0.4 1,181,309 - +0.1 1,181,328 + -0.7 1,181,328 + +0.5 1,181,329 - +1.0 1,181,361 + +0.0 1,181,361 + +0.9 1,181,361 + -2.4 1,181,361 + +0.6 1,181,362 - -1.0 1,181,362 - -0.2 1,181,366 - -0.3 1,181,366 - +0.1 1,181,500 + +1.1 1,181,500 + -0.4 1,181,500 + +0.8 1,181,500 + +0.6 1,181,500 + +1.4 1,181,501 - +1.7 1,181,630 - Pf1N1B4_1045 0.18 -0.1 1,181,630 - Pf1N1B4_1045 0.18 -0.2 1,181,694 + Pf1N1B4_1045 0.23 -0.1 1,181,695 - Pf1N1B4_1045 0.24 -0.5 1,181,836 + Pf1N1B4_1045 0.35 +1.7 1,181,836 + Pf1N1B4_1045 0.35 +0.3 1,181,837 - Pf1N1B4_1045 0.35 +0.9 1,181,983 - Pf1N1B4_1045 0.47 -0.2 1,181,983 - Pf1N1B4_1045 0.47 +0.1 1,182,265 + Pf1N1B4_1045 0.69 +0.3 1,182,266 - Pf1N1B4_1045 0.69 -1.9 1,182,266 - Pf1N1B4_1045 0.69 +0.8 1,182,266 - Pf1N1B4_1045 0.69 +0.6 1,182,266 - Pf1N1B4_1045 0.69 +0.0 1,182,266 - Pf1N1B4_1045 0.69 -1.0 1,182,266 - Pf1N1B4_1045 0.69 -1.2 1,182,409 + Pf1N1B4_1045 0.81 +0.6 1,182,410 - Pf1N1B4_1045 0.81 -0.2 1,182,410 - Pf1N1B4_1045 0.81 +0.1 1,182,654 + -0.4 1,182,654 + +1.7 1,182,704 + +0.8 1,182,704 + +0.9 1,182,705 - +0.2 1,182,705 - +0.3 1,182,717 + +0.1 1,182,717 + +0.8 1,182,733 - -0.0 1,182,767 + +0.0 1,182,768 - +0.5 1,182,768 - +1.1 1,182,786 + -2.2 1,182,787 - -0.1 1,182,820 + -0.5 1,182,820 + -1.7 1,182,821 - +0.7 1,182,821 - -1.7 1,182,821 - -0.1 1,182,821 - +0.6 1,182,821 - +0.6
Or see this region's nucleotide sequence