Experiment: H2 McCas- cells grown in liquid medium with neomycin
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS02640 and MMP_RS02645 are separated by 131 nucleotides MMP_RS02645 and MMP_RS08945 are separated by 112 nucleotides MMP_RS08945 and MMP_RS02650 are separated by 38 nucleotides MMP_RS02650 and MMP_RS02655 are separated by 145 nucleotides
MMP_RS02640: MMP_RS02640 - DUF6198 family protein, at 502,095 to 502,658
_RS02640
MMP_RS02645: MMP_RS02645 - ArsR family transcriptional regulator, at 502,790 to 503,530
_RS02645
MMP_RS08945: MMP_RS08945 - hypothetical protein, at 503,643 to 503,786
_RS08945
MMP_RS02650: MMP_RS02650 - flavodoxin family protein, at 503,825 to 504,376
_RS02650
MMP_RS02655: MMP_RS02655 - terpene cyclase/mutase family protein, at 504,522 to 506,024
_RS02655
Position (kb) 503
504 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4
5
6
7 at 502.759 kb on - strand at 502.766 kb on - strand at 502.766 kb on - strand at 502.767 kb on + strand at 502.771 kb on - strand at 502.771 kb on - strand at 502.806 kb on - strand at 502.829 kb on - strand at 502.850 kb on + strand at 502.995 kb on + strand, within MMP_RS02645 at 503.067 kb on + strand, within MMP_RS02645 at 503.215 kb on - strand, within MMP_RS02645 at 503.219 kb on - strand, within MMP_RS02645 at 503.252 kb on - strand, within MMP_RS02645 at 503.282 kb on - strand, within MMP_RS02645 at 503.295 kb on - strand, within MMP_RS02645 at 503.332 kb on - strand, within MMP_RS02645 at 503.372 kb on + strand, within MMP_RS02645 at 503.408 kb on - strand, within MMP_RS02645 at 503.409 kb on + strand, within MMP_RS02645 at 503.411 kb on + strand, within MMP_RS02645 at 503.535 kb on + strand at 503.546 kb on + strand at 503.553 kb on - strand at 503.556 kb on + strand at 503.562 kb on - strand at 503.563 kb on + strand at 503.612 kb on - strand at 503.613 kb on + strand at 503.633 kb on + strand at 503.640 kb on + strand at 503.642 kb on - strand at 503.643 kb on + strand at 503.673 kb on + strand, within MMP_RS08945 at 503.673 kb on + strand, within MMP_RS08945 at 503.714 kb on + strand, within MMP_RS08945 at 503.720 kb on - strand, within MMP_RS08945 at 503.720 kb on - strand, within MMP_RS08945 at 503.721 kb on + strand, within MMP_RS08945 at 503.721 kb on + strand, within MMP_RS08945 at 503.732 kb on - strand, within MMP_RS08945 at 503.732 kb on - strand, within MMP_RS08945 at 503.732 kb on - strand, within MMP_RS08945 at 503.733 kb on + strand, within MMP_RS08945 at 503.733 kb on + strand, within MMP_RS08945 at 503.733 kb on + strand, within MMP_RS08945 at 503.734 kb on - strand, within MMP_RS08945 at 503.734 kb on - strand, within MMP_RS08945 at 503.735 kb on + strand, within MMP_RS08945 at 503.735 kb on + strand, within MMP_RS08945 at 503.735 kb on + strand, within MMP_RS08945 at 503.759 kb on - strand, within MMP_RS08945 at 503.778 kb on + strand at 503.785 kb on + strand at 503.785 kb on + strand at 503.812 kb on + strand at 503.812 kb on + strand at 503.833 kb on - strand at 503.928 kb on - strand, within MMP_RS02650 at 503.929 kb on + strand, within MMP_RS02650 at 503.954 kb on + strand, within MMP_RS02650 at 503.954 kb on + strand, within MMP_RS02650 at 503.983 kb on - strand, within MMP_RS02650 at 504.017 kb on + strand, within MMP_RS02650 at 504.125 kb on - strand, within MMP_RS02650 at 504.132 kb on - strand, within MMP_RS02650 at 504.186 kb on + strand, within MMP_RS02650 at 504.224 kb on - strand, within MMP_RS02650 at 504.225 kb on + strand, within MMP_RS02650 at 504.264 kb on - strand, within MMP_RS02650 at 504.424 kb on - strand at 504.425 kb on + strand at 504.427 kb on + strand at 504.457 kb on + strand at 504.506 kb on - strand at 504.528 kb on + strand at 504.568 kb on - strand at 504.568 kb on - strand at 504.569 kb on + strand at 504.569 kb on + strand at 504.569 kb on + strand at 504.572 kb on - strand at 504.725 kb on + strand, within MMP_RS02655 at 504.746 kb on + strand, within MMP_RS02655
Per-strain Table
Position Strand Gene LocusTag Fraction H2 McCas- cells grown in liquid medium with neomycin remove 502,759 - -0.5 502,766 - +0.5 502,766 - -0.2 502,767 + +0.7 502,771 - -0.4 502,771 - +0.2 502,806 - +0.4 502,829 - -0.2 502,850 + +0.6 502,995 + MMP_RS02645 0.28 +0.3 503,067 + MMP_RS02645 0.37 +1.4 503,215 - MMP_RS02645 0.57 +0.2 503,219 - MMP_RS02645 0.58 +0.4 503,252 - MMP_RS02645 0.62 +0.5 503,282 - MMP_RS02645 0.66 -0.3 503,295 - MMP_RS02645 0.68 -2.6 503,332 - MMP_RS02645 0.73 +1.3 503,372 + MMP_RS02645 0.79 +1.4 503,408 - MMP_RS02645 0.83 +0.7 503,409 + MMP_RS02645 0.84 +1.6 503,411 + MMP_RS02645 0.84 +1.0 503,535 + +7.2 503,546 + +0.7 503,553 - -0.3 503,556 + -0.8 503,562 - -1.1 503,563 + -0.8 503,612 - -0.6 503,613 + +0.7 503,633 + +1.4 503,640 + -1.7 503,642 - +0.8 503,643 + +0.2 503,673 + MMP_RS08945 0.21 -0.2 503,673 + MMP_RS08945 0.21 -0.7 503,714 + MMP_RS08945 0.49 -0.1 503,720 - MMP_RS08945 0.53 +1.2 503,720 - MMP_RS08945 0.53 +0.5 503,721 + MMP_RS08945 0.54 +0.2 503,721 + MMP_RS08945 0.54 +0.5 503,732 - MMP_RS08945 0.62 +0.1 503,732 - MMP_RS08945 0.62 -2.8 503,732 - MMP_RS08945 0.62 -0.5 503,733 + MMP_RS08945 0.62 +0.2 503,733 + MMP_RS08945 0.62 +0.2 503,733 + MMP_RS08945 0.62 +3.4 503,734 - MMP_RS08945 0.63 -0.7 503,734 - MMP_RS08945 0.63 -1.3 503,735 + MMP_RS08945 0.64 -2.2 503,735 + MMP_RS08945 0.64 -1.7 503,735 + MMP_RS08945 0.64 -0.4 503,759 - MMP_RS08945 0.81 +0.1 503,778 + -0.6 503,785 + -0.5 503,785 + -0.4 503,812 + -0.6 503,812 + +0.1 503,833 - -0.2 503,928 - MMP_RS02650 0.19 -0.6 503,929 + MMP_RS02650 0.19 +0.1 503,954 + MMP_RS02650 0.23 -0.1 503,954 + MMP_RS02650 0.23 -0.9 503,983 - MMP_RS02650 0.29 +0.5 504,017 + MMP_RS02650 0.35 +0.7 504,125 - MMP_RS02650 0.54 +1.6 504,132 - MMP_RS02650 0.56 +0.7 504,186 + MMP_RS02650 0.65 -1.0 504,224 - MMP_RS02650 0.72 -0.1 504,225 + MMP_RS02650 0.72 +1.6 504,264 - MMP_RS02650 0.80 +0.1 504,424 - -0.1 504,425 + +1.0 504,427 + -0.6 504,457 + +0.8 504,506 - +0.5 504,528 + +0.2 504,568 - -0.3 504,568 - +0.4 504,569 + +0.1 504,569 + +0.8 504,569 + -1.1 504,572 - +0.5 504,725 + MMP_RS02655 0.14 -0.1 504,746 + MMP_RS02655 0.15 -1.3
Or see this region's nucleotide sequence