Experiment: H2 McCas- cells grown in liquid medium with neomycin
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS08305 and MMP_RS08310 are separated by 205 nucleotides MMP_RS08310 and MMP_RS08315 are separated by 10 nucleotides MMP_RS08315 and MMP_RS08320 are separated by 140 nucleotides
MMP_RS08305: MMP_RS08305 - aspartate--tRNA(Asn) ligase, at 1,561,499 to 1,562,815
_RS08305
MMP_RS08310: MMP_RS08310 - TMEM165/GDT1 family protein, at 1,563,021 to 1,563,284
_RS08310
MMP_RS08315: MMP_RS08315 - S-methyl-5-thioribose-1-phosphate isomerase, at 1,563,295 to 1,564,260
_RS08315
MMP_RS08320: MMP_RS08320 - molybdopterin molybdotransferase MoeA, at 1,564,401 to 1,565,609
_RS08320
Position (kb)
1563
1564
1565 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1562.586 kb on + strand at 1562.586 kb on + strand at 1562.832 kb on + strand at 1562.834 kb on + strand at 1562.895 kb on - strand at 1562.895 kb on - strand at 1562.896 kb on + strand at 1562.896 kb on + strand at 1562.907 kb on - strand at 1562.908 kb on + strand at 1562.908 kb on + strand at 1562.912 kb on - strand at 1562.913 kb on + strand at 1562.913 kb on + strand at 1562.922 kb on + strand at 1562.925 kb on + strand at 1562.951 kb on + strand at 1562.961 kb on + strand at 1562.987 kb on + strand at 1563.003 kb on + strand at 1563.037 kb on + strand at 1563.058 kb on + strand, within MMP_RS08310 at 1563.265 kb on + strand at 1563.300 kb on - strand at 1563.340 kb on + strand at 1563.425 kb on + strand, within MMP_RS08315 at 1563.457 kb on + strand, within MMP_RS08315 at 1563.745 kb on - strand, within MMP_RS08315 at 1563.746 kb on + strand, within MMP_RS08315 at 1564.169 kb on - strand at 1564.210 kb on - strand at 1564.210 kb on - strand at 1564.239 kb on - strand at 1564.239 kb on - strand at 1564.240 kb on + strand at 1564.274 kb on - strand at 1564.274 kb on - strand at 1564.275 kb on + strand at 1564.275 kb on + strand at 1564.275 kb on + strand at 1564.277 kb on + strand at 1564.278 kb on - strand at 1564.278 kb on - strand at 1564.279 kb on + strand at 1564.290 kb on + strand at 1564.299 kb on + strand at 1564.316 kb on - strand at 1564.482 kb on + strand at 1564.744 kb on + strand, within MMP_RS08320 at 1564.745 kb on - strand, within MMP_RS08320 at 1564.745 kb on - strand, within MMP_RS08320 at 1564.775 kb on + strand, within MMP_RS08320 at 1564.778 kb on - strand, within MMP_RS08320 at 1564.779 kb on + strand, within MMP_RS08320 at 1564.779 kb on + strand, within MMP_RS08320 at 1565.018 kb on - strand, within MMP_RS08320 at 1565.051 kb on + strand, within MMP_RS08320
Per-strain Table
Position Strand Gene LocusTag Fraction H2 McCas- cells grown in liquid medium with neomycin remove 1,562,586 + -0.0 1,562,586 + +1.7 1,562,832 + +0.5 1,562,834 + +1.5 1,562,895 - -1.3 1,562,895 - +0.4 1,562,896 + +1.1 1,562,896 + +0.0 1,562,907 - +0.8 1,562,908 + +0.1 1,562,908 + -0.2 1,562,912 - -0.4 1,562,913 + -0.2 1,562,913 + +0.0 1,562,922 + -0.1 1,562,925 + -0.0 1,562,951 + -0.1 1,562,961 + -0.0 1,562,987 + +0.0 1,563,003 + +0.2 1,563,037 + -0.0 1,563,058 + MMP_RS08310 0.14 -0.4 1,563,265 + -0.7 1,563,300 - +2.9 1,563,340 + +0.7 1,563,425 + MMP_RS08315 0.13 -0.5 1,563,457 + MMP_RS08315 0.17 +3.3 1,563,745 - MMP_RS08315 0.47 -0.3 1,563,746 + MMP_RS08315 0.47 +0.2 1,564,169 - +0.1 1,564,210 - -0.7 1,564,210 - +0.3 1,564,239 - -1.8 1,564,239 - -0.0 1,564,240 + -0.2 1,564,274 - -1.0 1,564,274 - +0.1 1,564,275 + +0.5 1,564,275 + -1.2 1,564,275 + +0.8 1,564,277 + -1.8 1,564,278 - -0.0 1,564,278 - +0.5 1,564,279 + +1.2 1,564,290 + +0.1 1,564,299 + +0.1 1,564,316 - -0.5 1,564,482 + -0.5 1,564,744 + MMP_RS08320 0.28 -1.0 1,564,745 - MMP_RS08320 0.28 -0.1 1,564,745 - MMP_RS08320 0.28 +1.0 1,564,775 + MMP_RS08320 0.31 -1.0 1,564,778 - MMP_RS08320 0.31 +0.6 1,564,779 + MMP_RS08320 0.31 -0.9 1,564,779 + MMP_RS08320 0.31 +0.5 1,565,018 - MMP_RS08320 0.51 +0.2 1,565,051 + MMP_RS08320 0.54 +0.3
Or see this region's nucleotide sequence