Strain Fitness in Methanococcus maripaludis S2 around MMP_RS08315

Experiment: H2 McCas- cells grown in liquid medium with neomycin

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntMMP_RS08305 and MMP_RS08310 are separated by 205 nucleotidesMMP_RS08310 and MMP_RS08315 are separated by 10 nucleotidesMMP_RS08315 and MMP_RS08320 are separated by 140 nucleotides MMP_RS08305: MMP_RS08305 - aspartate--tRNA(Asn) ligase, at 1,561,499 to 1,562,815 _RS08305 MMP_RS08310: MMP_RS08310 - TMEM165/GDT1 family protein, at 1,563,021 to 1,563,284 _RS08310 MMP_RS08315: MMP_RS08315 - S-methyl-5-thioribose-1-phosphate isomerase, at 1,563,295 to 1,564,260 _RS08315 MMP_RS08320: MMP_RS08320 - molybdopterin molybdotransferase MoeA, at 1,564,401 to 1,565,609 _RS08320 Position (kb) 1563 1564 1565Strain fitness (log2 ratio) -2 -1 0 1 2 3at 1562.586 kb on + strandat 1562.586 kb on + strandat 1562.832 kb on + strandat 1562.834 kb on + strandat 1562.895 kb on - strandat 1562.895 kb on - strandat 1562.896 kb on + strandat 1562.896 kb on + strandat 1562.907 kb on - strandat 1562.908 kb on + strandat 1562.908 kb on + strandat 1562.912 kb on - strandat 1562.913 kb on + strandat 1562.913 kb on + strandat 1562.922 kb on + strandat 1562.925 kb on + strandat 1562.951 kb on + strandat 1562.961 kb on + strandat 1562.987 kb on + strandat 1563.003 kb on + strandat 1563.037 kb on + strandat 1563.058 kb on + strand, within MMP_RS08310at 1563.265 kb on + strandat 1563.300 kb on - strandat 1563.340 kb on + strandat 1563.425 kb on + strand, within MMP_RS08315at 1563.457 kb on + strand, within MMP_RS08315at 1563.745 kb on - strand, within MMP_RS08315at 1563.746 kb on + strand, within MMP_RS08315at 1564.169 kb on - strandat 1564.210 kb on - strandat 1564.210 kb on - strandat 1564.239 kb on - strandat 1564.239 kb on - strandat 1564.240 kb on + strandat 1564.274 kb on - strandat 1564.274 kb on - strandat 1564.275 kb on + strandat 1564.275 kb on + strandat 1564.275 kb on + strandat 1564.277 kb on + strandat 1564.278 kb on - strandat 1564.278 kb on - strandat 1564.279 kb on + strandat 1564.290 kb on + strandat 1564.299 kb on + strandat 1564.316 kb on - strandat 1564.482 kb on + strandat 1564.744 kb on + strand, within MMP_RS08320at 1564.745 kb on - strand, within MMP_RS08320at 1564.745 kb on - strand, within MMP_RS08320at 1564.775 kb on + strand, within MMP_RS08320at 1564.778 kb on - strand, within MMP_RS08320at 1564.779 kb on + strand, within MMP_RS08320at 1564.779 kb on + strand, within MMP_RS08320at 1565.018 kb on - strand, within MMP_RS08320at 1565.051 kb on + strand, within MMP_RS08320

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Per-strain Table

Position Strand Gene LocusTag Fraction H2 McCas- cells grown in liquid medium with neomycin
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1,562,586 + -0.0
1,562,586 + +1.7
1,562,832 + +0.5
1,562,834 + +1.5
1,562,895 - -1.3
1,562,895 - +0.4
1,562,896 + +1.1
1,562,896 + +0.0
1,562,907 - +0.8
1,562,908 + +0.1
1,562,908 + -0.2
1,562,912 - -0.4
1,562,913 + -0.2
1,562,913 + +0.0
1,562,922 + -0.1
1,562,925 + -0.0
1,562,951 + -0.1
1,562,961 + -0.0
1,562,987 + +0.0
1,563,003 + +0.2
1,563,037 + -0.0
1,563,058 + MMP_RS08310 0.14 -0.4
1,563,265 + -0.7
1,563,300 - +2.9
1,563,340 + +0.7
1,563,425 + MMP_RS08315 0.13 -0.5
1,563,457 + MMP_RS08315 0.17 +3.3
1,563,745 - MMP_RS08315 0.47 -0.3
1,563,746 + MMP_RS08315 0.47 +0.2
1,564,169 - +0.1
1,564,210 - -0.7
1,564,210 - +0.3
1,564,239 - -1.8
1,564,239 - -0.0
1,564,240 + -0.2
1,564,274 - -1.0
1,564,274 - +0.1
1,564,275 + +0.5
1,564,275 + -1.2
1,564,275 + +0.8
1,564,277 + -1.8
1,564,278 - -0.0
1,564,278 - +0.5
1,564,279 + +1.2
1,564,290 + +0.1
1,564,299 + +0.1
1,564,316 - -0.5
1,564,482 + -0.5
1,564,744 + MMP_RS08320 0.28 -1.0
1,564,745 - MMP_RS08320 0.28 -0.1
1,564,745 - MMP_RS08320 0.28 +1.0
1,564,775 + MMP_RS08320 0.31 -1.0
1,564,778 - MMP_RS08320 0.31 +0.6
1,564,779 + MMP_RS08320 0.31 -0.9
1,564,779 + MMP_RS08320 0.31 +0.5
1,565,018 - MMP_RS08320 0.51 +0.2
1,565,051 + MMP_RS08320 0.54 +0.3

Or see this region's nucleotide sequence