Experiment: H2 McCas- cells grown in liquid medium with neomycin
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS07905 and MMP_RS07910 overlap by 4 nucleotides MMP_RS07910 and MMP_RS07915 are separated by 25 nucleotides MMP_RS07915 and MMP_RS07920 are separated by 153 nucleotides MMP_RS07920 and MMP_RS07925 are separated by 60 nucleotides
MMP_RS07905: MMP_RS07905 - DUF3343 domain-containing protein, at 1,496,007 to 1,496,567
_RS07905
MMP_RS07910: MMP_RS07910 - NAD(P)H-hydrate dehydratase, at 1,496,564 to 1,497,385
_RS07910
MMP_RS07915: MMP_RS07915 - DUF4870 domain-containing protein, at 1,497,411 to 1,497,728
_RS07915
MMP_RS07920: MMP_RS07920 - tRNA-Ile, at 1,497,882 to 1,497,956
_RS07920
MMP_RS07925: MMP_RS07925 - haloacid dehalogenase, at 1,498,017 to 1,498,607
_RS07925
Position (kb)
1497
1498 Strain fitness (log2 ratio)
-1
0
1
2 at 1496.422 kb on + strand, within MMP_RS07905 at 1496.450 kb on - strand, within MMP_RS07905 at 1496.474 kb on - strand, within MMP_RS07905 at 1496.747 kb on + strand, within MMP_RS07910 at 1496.853 kb on - strand, within MMP_RS07910 at 1497.066 kb on - strand, within MMP_RS07910 at 1497.078 kb on + strand, within MMP_RS07910 at 1497.150 kb on - strand, within MMP_RS07910 at 1497.150 kb on - strand, within MMP_RS07910 at 1497.213 kb on - strand, within MMP_RS07910 at 1497.251 kb on + strand, within MMP_RS07910 at 1497.289 kb on - strand, within MMP_RS07910 at 1497.382 kb on + strand at 1497.382 kb on + strand at 1497.382 kb on + strand at 1497.387 kb on + strand at 1497.442 kb on + strand at 1497.516 kb on - strand, within MMP_RS07915 at 1497.552 kb on + strand, within MMP_RS07915 at 1497.651 kb on - strand, within MMP_RS07915 at 1497.657 kb on - strand, within MMP_RS07915 at 1497.658 kb on + strand, within MMP_RS07915 at 1497.683 kb on + strand, within MMP_RS07915 at 1497.721 kb on + strand at 1497.757 kb on + strand at 1497.761 kb on + strand at 1497.761 kb on - strand at 1497.761 kb on - strand at 1497.761 kb on - strand at 1497.761 kb on - strand at 1497.762 kb on + strand at 1497.762 kb on + strand at 1497.783 kb on + strand at 1497.850 kb on + strand at 1498.074 kb on - strand at 1498.075 kb on + strand at 1498.075 kb on + strand at 1498.141 kb on + strand, within MMP_RS07925 at 1498.188 kb on + strand, within MMP_RS07925 at 1498.299 kb on - strand, within MMP_RS07925 at 1498.300 kb on + strand, within MMP_RS07925 at 1498.355 kb on + strand, within MMP_RS07925 at 1498.639 kb on - strand at 1498.669 kb on + strand at 1498.704 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 McCas- cells grown in liquid medium with neomycin remove 1,496,422 + MMP_RS07905 0.74 -0.0 1,496,450 - MMP_RS07905 0.79 +0.3 1,496,474 - MMP_RS07905 0.83 -0.8 1,496,747 + MMP_RS07910 0.22 +0.3 1,496,853 - MMP_RS07910 0.35 -0.7 1,497,066 - MMP_RS07910 0.61 -0.3 1,497,078 + MMP_RS07910 0.63 -1.0 1,497,150 - MMP_RS07910 0.71 +0.6 1,497,150 - MMP_RS07910 0.71 -0.2 1,497,213 - MMP_RS07910 0.79 -0.6 1,497,251 + MMP_RS07910 0.84 +0.6 1,497,289 - MMP_RS07910 0.88 +1.1 1,497,382 + -0.5 1,497,382 + +2.3 1,497,382 + +0.2 1,497,387 + +0.9 1,497,442 + -0.8 1,497,516 - MMP_RS07915 0.33 -0.2 1,497,552 + MMP_RS07915 0.44 +0.7 1,497,651 - MMP_RS07915 0.75 -0.2 1,497,657 - MMP_RS07915 0.77 -0.5 1,497,658 + MMP_RS07915 0.78 +0.5 1,497,683 + MMP_RS07915 0.86 -0.6 1,497,721 + -0.6 1,497,757 + -0.0 1,497,761 + +0.5 1,497,761 - +0.1 1,497,761 - -0.8 1,497,761 - -1.3 1,497,761 - -0.2 1,497,762 + -0.5 1,497,762 + -0.4 1,497,783 + -0.2 1,497,850 + +1.3 1,498,074 - +0.8 1,498,075 + +0.5 1,498,075 + +0.3 1,498,141 + MMP_RS07925 0.21 +1.7 1,498,188 + MMP_RS07925 0.29 -1.4 1,498,299 - MMP_RS07925 0.48 -0.9 1,498,300 + MMP_RS07925 0.48 +0.1 1,498,355 + MMP_RS07925 0.57 -0.0 1,498,639 - -0.0 1,498,669 + -0.6 1,498,704 - +1.0
Or see this region's nucleotide sequence