Strain Fitness in Methanococcus maripaludis S2 around MMP_RS06840

Experiment: H2 McCas- cells grown in liquid medium with neomycin

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntMMP_RS06825 and MMP_RS06830 are separated by 20 nucleotidesMMP_RS06830 and MMP_RS06835 are separated by 13 nucleotidesMMP_RS06835 and MMP_RS06840 are separated by 38 nucleotidesMMP_RS06840 and MMP_RS06845 are separated by 37 nucleotidesMMP_RS06845 and MMP_RS06850 are separated by 37 nucleotidesMMP_RS06850 and MMP_RS06855 are separated by 31 nucleotides MMP_RS06825: MMP_RS06825 - 50S ribosomal protein L18e, at 1,307,955 to 1,308,317 _RS06825 MMP_RS06830: MMP_RS06830 - 50S ribosomal protein L13, at 1,308,338 to 1,308,751 _RS06830 MMP_RS06835: MMP_RS06835 - 30S ribosomal protein S9, at 1,308,765 to 1,309,169 _RS06835 MMP_RS06840: MMP_RS06840 - DNA-directed RNA polymerase subunit N, at 1,309,208 to 1,309,414 _RS06840 MMP_RS06845: MMP_RS06845 - tRNA-Pro, at 1,309,452 to 1,309,525 _RS06845 MMP_RS06850: MMP_RS06850 - DNA-directed RNA polymerase subunit K, at 1,309,563 to 1,309,736 _RS06850 MMP_RS06855: MMP_RS06855 - hypothetical protein, at 1,309,768 to 1,310,703 _RS06855 Position (kb) 1309 1310Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 1309.168 kb on + strandat 1309.387 kb on + strand, within MMP_RS06840at 1309.407 kb on + strandat 1309.425 kb on + strandat 1309.432 kb on - strandat 1309.436 kb on + strandat 1309.540 kb on + strandat 1309.672 kb on - strandat 1309.742 kb on - strandat 1309.836 kb on + strandat 1309.846 kb on + strandat 1309.870 kb on - strand, within MMP_RS06855at 1309.925 kb on - strand, within MMP_RS06855at 1309.957 kb on - strand, within MMP_RS06855at 1309.985 kb on - strand, within MMP_RS06855at 1310.195 kb on - strand, within MMP_RS06855at 1310.199 kb on - strand, within MMP_RS06855at 1310.199 kb on - strand, within MMP_RS06855at 1310.204 kb on + strand, within MMP_RS06855at 1310.266 kb on + strand, within MMP_RS06855at 1310.319 kb on - strand, within MMP_RS06855at 1310.319 kb on - strand, within MMP_RS06855at 1310.374 kb on + strand, within MMP_RS06855at 1310.402 kb on - strand, within MMP_RS06855

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Per-strain Table

Position Strand Gene LocusTag Fraction H2 McCas- cells grown in liquid medium with neomycin
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1,309,168 + -0.1
1,309,387 + MMP_RS06840 0.86 -1.4
1,309,407 + -1.0
1,309,425 + -3.0
1,309,432 - +1.0
1,309,436 + -1.4
1,309,540 + +0.7
1,309,672 - +3.3
1,309,742 - -0.5
1,309,836 + -0.1
1,309,846 + +0.1
1,309,870 - MMP_RS06855 0.11 +1.0
1,309,925 - MMP_RS06855 0.17 +0.1
1,309,957 - MMP_RS06855 0.20 -0.1
1,309,985 - MMP_RS06855 0.23 +0.3
1,310,195 - MMP_RS06855 0.46 -0.1
1,310,199 - MMP_RS06855 0.46 -0.8
1,310,199 - MMP_RS06855 0.46 +0.9
1,310,204 + MMP_RS06855 0.47 +0.8
1,310,266 + MMP_RS06855 0.53 +1.8
1,310,319 - MMP_RS06855 0.59 +0.4
1,310,319 - MMP_RS06855 0.59 -0.0
1,310,374 + MMP_RS06855 0.65 -1.1
1,310,402 - MMP_RS06855 0.68 +0.6

Or see this region's nucleotide sequence