Strain Fitness in Methanococcus maripaludis S2 around MMP_RS06765

Experiment: H2 McCas- cells grown in liquid medium with neomycin

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntMMP_RS06750 and MMP_RS06755 are separated by 29 nucleotidesMMP_RS06755 and MMP_RS06760 are separated by 17 nucleotidesMMP_RS06760 and MMP_RS06765 are separated by 8 nucleotidesMMP_RS06765 and MMP_RS06770 are separated by 36 nucleotides MMP_RS06750: MMP_RS06750 - IMP cyclohydrolase, at 1,296,054 to 1,296,659 _RS06750 MMP_RS06755: MMP_RS06755 - 4Fe-4S binding protein, at 1,296,689 to 1,296,847 _RS06755 MMP_RS06760: MMP_RS06760 - hypothetical protein, at 1,296,865 to 1,297,491 _RS06760 MMP_RS06765: MMP_RS06765 - flap endonuclease-1, at 1,297,500 to 1,298,474 _RS06765 MMP_RS06770: MMP_RS06770 - DHH family phosphoesterase, at 1,298,511 to 1,300,796 _RS06770 Position (kb) 1297 1298 1299Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 1296.655 kb on - strandat 1296.656 kb on + strandat 1296.729 kb on + strandat 1296.824 kb on - strandat 1296.861 kb on - strandat 1296.862 kb on + strandat 1296.919 kb on + strandat 1297.159 kb on + strand, within MMP_RS06760at 1297.263 kb on - strand, within MMP_RS06760at 1297.388 kb on + strand, within MMP_RS06760at 1297.496 kb on + strandat 1297.650 kb on - strand, within MMP_RS06765at 1297.706 kb on + strand, within MMP_RS06765at 1297.965 kb on - strand, within MMP_RS06765at 1297.966 kb on + strand, within MMP_RS06765at 1297.966 kb on + strand, within MMP_RS06765at 1297.967 kb on - strand, within MMP_RS06765at 1298.034 kb on - strand, within MMP_RS06765at 1298.034 kb on - strand, within MMP_RS06765at 1298.035 kb on + strand, within MMP_RS06765at 1298.038 kb on - strand, within MMP_RS06765at 1298.411 kb on - strandat 1298.661 kb on - strandat 1298.661 kb on - strandat 1298.661 kb on - strandat 1298.661 kb on - strandat 1298.662 kb on + strandat 1298.662 kb on + strandat 1298.662 kb on + strandat 1298.663 kb on - strandat 1299.163 kb on - strand, within MMP_RS06770at 1299.249 kb on - strand, within MMP_RS06770

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Per-strain Table

Position Strand Gene LocusTag Fraction H2 McCas- cells grown in liquid medium with neomycin
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1,296,655 - -0.8
1,296,656 + -0.6
1,296,729 + -2.1
1,296,824 - -0.7
1,296,861 - +0.9
1,296,862 + +3.3
1,296,919 + -0.8
1,297,159 + MMP_RS06760 0.47 -0.4
1,297,263 - MMP_RS06760 0.63 +0.6
1,297,388 + MMP_RS06760 0.83 +1.0
1,297,496 + +0.1
1,297,650 - MMP_RS06765 0.15 -1.1
1,297,706 + MMP_RS06765 0.21 -3.0
1,297,965 - MMP_RS06765 0.48 -0.5
1,297,966 + MMP_RS06765 0.48 +0.0
1,297,966 + MMP_RS06765 0.48 -0.2
1,297,967 - MMP_RS06765 0.48 -0.6
1,298,034 - MMP_RS06765 0.55 -0.7
1,298,034 - MMP_RS06765 0.55 -1.1
1,298,035 + MMP_RS06765 0.55 -0.3
1,298,038 - MMP_RS06765 0.55 +0.2
1,298,411 - +0.2
1,298,661 - -0.0
1,298,661 - -2.2
1,298,661 - +0.3
1,298,661 - -0.8
1,298,662 + -0.5
1,298,662 + +0.1
1,298,662 + +0.0
1,298,663 - -0.6
1,299,163 - MMP_RS06770 0.29 +0.3
1,299,249 - MMP_RS06770 0.32 +0.1

Or see this region's nucleotide sequence