Experiment: H2 McCas- cells grown in liquid medium with neomycin
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS06750 and MMP_RS06755 are separated by 29 nucleotides MMP_RS06755 and MMP_RS06760 are separated by 17 nucleotides MMP_RS06760 and MMP_RS06765 are separated by 8 nucleotides MMP_RS06765 and MMP_RS06770 are separated by 36 nucleotides
MMP_RS06750: MMP_RS06750 - IMP cyclohydrolase, at 1,296,054 to 1,296,659
_RS06750
MMP_RS06755: MMP_RS06755 - 4Fe-4S binding protein, at 1,296,689 to 1,296,847
_RS06755
MMP_RS06760: MMP_RS06760 - hypothetical protein, at 1,296,865 to 1,297,491
_RS06760
MMP_RS06765: MMP_RS06765 - flap endonuclease-1, at 1,297,500 to 1,298,474
_RS06765
MMP_RS06770: MMP_RS06770 - DHH family phosphoesterase, at 1,298,511 to 1,300,796
_RS06770
Position (kb)
1297
1298
1299 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1296.655 kb on - strand at 1296.656 kb on + strand at 1296.729 kb on + strand at 1296.824 kb on - strand at 1296.861 kb on - strand at 1296.862 kb on + strand at 1296.919 kb on + strand at 1297.159 kb on + strand, within MMP_RS06760 at 1297.263 kb on - strand, within MMP_RS06760 at 1297.388 kb on + strand, within MMP_RS06760 at 1297.496 kb on + strand at 1297.650 kb on - strand, within MMP_RS06765 at 1297.706 kb on + strand, within MMP_RS06765 at 1297.965 kb on - strand, within MMP_RS06765 at 1297.966 kb on + strand, within MMP_RS06765 at 1297.966 kb on + strand, within MMP_RS06765 at 1297.967 kb on - strand, within MMP_RS06765 at 1298.034 kb on - strand, within MMP_RS06765 at 1298.034 kb on - strand, within MMP_RS06765 at 1298.035 kb on + strand, within MMP_RS06765 at 1298.038 kb on - strand, within MMP_RS06765 at 1298.411 kb on - strand at 1298.661 kb on - strand at 1298.661 kb on - strand at 1298.661 kb on - strand at 1298.661 kb on - strand at 1298.662 kb on + strand at 1298.662 kb on + strand at 1298.662 kb on + strand at 1298.663 kb on - strand at 1299.163 kb on - strand, within MMP_RS06770 at 1299.249 kb on - strand, within MMP_RS06770
Per-strain Table
Position Strand Gene LocusTag Fraction H2 McCas- cells grown in liquid medium with neomycin remove 1,296,655 - -0.8 1,296,656 + -0.6 1,296,729 + -2.1 1,296,824 - -0.7 1,296,861 - +0.9 1,296,862 + +3.3 1,296,919 + -0.8 1,297,159 + MMP_RS06760 0.47 -0.4 1,297,263 - MMP_RS06760 0.63 +0.6 1,297,388 + MMP_RS06760 0.83 +1.0 1,297,496 + +0.1 1,297,650 - MMP_RS06765 0.15 -1.1 1,297,706 + MMP_RS06765 0.21 -3.0 1,297,965 - MMP_RS06765 0.48 -0.5 1,297,966 + MMP_RS06765 0.48 +0.0 1,297,966 + MMP_RS06765 0.48 -0.2 1,297,967 - MMP_RS06765 0.48 -0.6 1,298,034 - MMP_RS06765 0.55 -0.7 1,298,034 - MMP_RS06765 0.55 -1.1 1,298,035 + MMP_RS06765 0.55 -0.3 1,298,038 - MMP_RS06765 0.55 +0.2 1,298,411 - +0.2 1,298,661 - -0.0 1,298,661 - -2.2 1,298,661 - +0.3 1,298,661 - -0.8 1,298,662 + -0.5 1,298,662 + +0.1 1,298,662 + +0.0 1,298,663 - -0.6 1,299,163 - MMP_RS06770 0.29 +0.3 1,299,249 - MMP_RS06770 0.32 +0.1
Or see this region's nucleotide sequence