Strain Fitness in Methanococcus maripaludis S2 around MMP_RS06385

Experiment: H2 McCas- cells grown in liquid medium with neomycin

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntMMP_RS06380 and MMP_RS06385 are separated by 5 nucleotidesMMP_RS06385 and MMP_RS06390 are separated by 10 nucleotidesMMP_RS06390 and MMP_RS06395 are separated by 52 nucleotides MMP_RS06380: MMP_RS06380 - O-phospho-L-seryl-tRNA:Cys-tRNA synthase, at 1,225,667 to 1,226,809 _RS06380 MMP_RS06385: MMP_RS06385 - hypothetical protein, at 1,226,815 to 1,227,174 _RS06385 MMP_RS06390: MMP_RS06390 - quinolinate synthase NadA, at 1,227,185 to 1,228,102 _RS06390 MMP_RS06395: MMP_RS06395 - ThiF family adenylyltransferase, at 1,228,155 to 1,228,769 _RS06395 Position (kb) 1226 1227 1228Strain fitness (log2 ratio) -1 0 1 2at 1225.902 kb on - strandat 1226.409 kb on - strandat 1226.873 kb on + strand, within MMP_RS06385at 1226.978 kb on - strand, within MMP_RS06385at 1226.990 kb on + strand, within MMP_RS06385at 1227.074 kb on - strand, within MMP_RS06385at 1227.075 kb on + strand, within MMP_RS06385at 1227.080 kb on - strand, within MMP_RS06385at 1227.173 kb on + strandat 1227.710 kb on + strandat 1227.766 kb on - strandat 1227.771 kb on + strandat 1227.779 kb on - strandat 1227.821 kb on + strandat 1228.036 kb on + strandat 1228.094 kb on - strandat 1228.094 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction H2 McCas- cells grown in liquid medium with neomycin
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1,225,902 - +1.8
1,226,409 - +1.6
1,226,873 + MMP_RS06385 0.16 -0.8
1,226,978 - MMP_RS06385 0.45 -0.7
1,226,990 + MMP_RS06385 0.49 -0.5
1,227,074 - MMP_RS06385 0.72 -0.4
1,227,075 + MMP_RS06385 0.72 +1.2
1,227,080 - MMP_RS06385 0.74 -0.7
1,227,173 + -0.3
1,227,710 + +1.8
1,227,766 - +1.8
1,227,771 + +1.0
1,227,779 - +1.8
1,227,821 + -0.1
1,228,036 + +0.2
1,228,094 - +1.4
1,228,094 - -0.1

Or see this region's nucleotide sequence