Experiment: H2 McCas- cells grown in liquid medium with neomycin
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS05995 and MMP_RS06000 are separated by 24 nucleotides MMP_RS06000 and MMP_RS06005 are separated by 127 nucleotides MMP_RS06005 and MMP_RS06010 are separated by 15 nucleotides
MMP_RS05995: MMP_RS05995 - FprA family A-type flavoprotein, at 1,146,467 to 1,147,630
_RS05995
MMP_RS06000: MMP_RS06000 - divalent-cation tolerance protein CutA, at 1,147,655 to 1,147,969
_RS06000
MMP_RS06005: MMP_RS06005 - heavy-metal-associated domain-containing protein, at 1,148,097 to 1,148,315
_RS06005
MMP_RS06010: MMP_RS06010 - heavy metal translocating P-type ATPase, at 1,148,331 to 1,150,502
_RS06010
Position (kb)
1148
1149 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1147.107 kb on - strand, within MMP_RS05995 at 1147.110 kb on - strand, within MMP_RS05995 at 1147.110 kb on - strand, within MMP_RS05995 at 1147.110 kb on - strand, within MMP_RS05995 at 1147.111 kb on + strand, within MMP_RS05995 at 1147.111 kb on + strand, within MMP_RS05995 at 1147.226 kb on - strand, within MMP_RS05995 at 1147.321 kb on + strand, within MMP_RS05995 at 1147.392 kb on - strand, within MMP_RS05995 at 1147.421 kb on - strand, within MMP_RS05995 at 1147.492 kb on - strand, within MMP_RS05995 at 1147.504 kb on - strand, within MMP_RS05995 at 1147.510 kb on - strand, within MMP_RS05995 at 1147.511 kb on + strand, within MMP_RS05995 at 1147.555 kb on + strand at 1147.621 kb on - strand at 1147.621 kb on - strand at 1147.622 kb on + strand at 1147.676 kb on - strand at 1147.712 kb on + strand, within MMP_RS06000 at 1147.716 kb on - strand, within MMP_RS06000 at 1147.717 kb on + strand, within MMP_RS06000 at 1147.717 kb on + strand, within MMP_RS06000 at 1147.778 kb on - strand, within MMP_RS06000 at 1147.778 kb on - strand, within MMP_RS06000 at 1147.779 kb on + strand, within MMP_RS06000 at 1147.808 kb on + strand, within MMP_RS06000 at 1147.968 kb on + strand at 1147.989 kb on - strand at 1148.019 kb on - strand at 1148.020 kb on + strand at 1148.029 kb on + strand at 1148.029 kb on + strand at 1148.066 kb on - strand at 1148.066 kb on - strand at 1148.066 kb on - strand at 1148.228 kb on + strand, within MMP_RS06005 at 1148.287 kb on - strand, within MMP_RS06005 at 1148.288 kb on + strand, within MMP_RS06005 at 1148.288 kb on + strand, within MMP_RS06005 at 1148.636 kb on - strand, within MMP_RS06010 at 1148.643 kb on - strand, within MMP_RS06010 at 1148.643 kb on - strand, within MMP_RS06010 at 1148.643 kb on - strand, within MMP_RS06010 at 1148.643 kb on - strand, within MMP_RS06010 at 1148.660 kb on - strand, within MMP_RS06010 at 1148.661 kb on + strand, within MMP_RS06010 at 1148.667 kb on - strand, within MMP_RS06010 at 1148.703 kb on - strand, within MMP_RS06010 at 1148.703 kb on - strand, within MMP_RS06010 at 1148.751 kb on + strand, within MMP_RS06010 at 1148.771 kb on - strand, within MMP_RS06010 at 1148.799 kb on - strand, within MMP_RS06010 at 1148.800 kb on + strand, within MMP_RS06010 at 1148.801 kb on - strand, within MMP_RS06010 at 1148.902 kb on - strand, within MMP_RS06010 at 1149.179 kb on - strand, within MMP_RS06010 at 1149.200 kb on + strand, within MMP_RS06010 at 1149.229 kb on - strand, within MMP_RS06010 at 1149.241 kb on - strand, within MMP_RS06010
Per-strain Table
Position Strand Gene LocusTag Fraction H2 McCas- cells grown in liquid medium with neomycin remove 1,147,107 - MMP_RS05995 0.55 -0.5 1,147,110 - MMP_RS05995 0.55 -0.2 1,147,110 - MMP_RS05995 0.55 -0.1 1,147,110 - MMP_RS05995 0.55 -0.1 1,147,111 + MMP_RS05995 0.55 -0.3 1,147,111 + MMP_RS05995 0.55 -0.0 1,147,226 - MMP_RS05995 0.65 -0.5 1,147,321 + MMP_RS05995 0.73 -0.1 1,147,392 - MMP_RS05995 0.79 -1.1 1,147,421 - MMP_RS05995 0.82 +0.5 1,147,492 - MMP_RS05995 0.88 +0.1 1,147,504 - MMP_RS05995 0.89 -1.1 1,147,510 - MMP_RS05995 0.90 -0.2 1,147,511 + MMP_RS05995 0.90 +0.2 1,147,555 + -0.6 1,147,621 - +0.2 1,147,621 - -0.3 1,147,622 + +0.5 1,147,676 - -2.0 1,147,712 + MMP_RS06000 0.18 -0.0 1,147,716 - MMP_RS06000 0.19 -0.1 1,147,717 + MMP_RS06000 0.20 -0.6 1,147,717 + MMP_RS06000 0.20 +3.4 1,147,778 - MMP_RS06000 0.39 +0.1 1,147,778 - MMP_RS06000 0.39 +0.4 1,147,779 + MMP_RS06000 0.39 -0.8 1,147,808 + MMP_RS06000 0.49 +0.2 1,147,968 + +0.3 1,147,989 - +0.5 1,148,019 - +0.6 1,148,020 + +0.5 1,148,029 + +0.3 1,148,029 + +0.2 1,148,066 - -0.7 1,148,066 - -1.6 1,148,066 - +0.2 1,148,228 + MMP_RS06005 0.60 -0.3 1,148,287 - MMP_RS06005 0.87 -0.3 1,148,288 + MMP_RS06005 0.87 -0.4 1,148,288 + MMP_RS06005 0.87 -0.3 1,148,636 - MMP_RS06010 0.14 -0.1 1,148,643 - MMP_RS06010 0.14 +1.5 1,148,643 - MMP_RS06010 0.14 -0.8 1,148,643 - MMP_RS06010 0.14 +0.3 1,148,643 - MMP_RS06010 0.14 +0.2 1,148,660 - MMP_RS06010 0.15 -0.7 1,148,661 + MMP_RS06010 0.15 -0.6 1,148,667 - MMP_RS06010 0.15 +1.4 1,148,703 - MMP_RS06010 0.17 +0.4 1,148,703 - MMP_RS06010 0.17 -0.3 1,148,751 + MMP_RS06010 0.19 +0.0 1,148,771 - MMP_RS06010 0.20 -1.0 1,148,799 - MMP_RS06010 0.22 +0.8 1,148,800 + MMP_RS06010 0.22 +0.1 1,148,801 - MMP_RS06010 0.22 +0.6 1,148,902 - MMP_RS06010 0.26 -0.1 1,149,179 - MMP_RS06010 0.39 +0.1 1,149,200 + MMP_RS06010 0.40 +0.0 1,149,229 - MMP_RS06010 0.41 -0.1 1,149,241 - MMP_RS06010 0.42 -0.0
Or see this region's nucleotide sequence